BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0006.p (133 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310508|ref|YP_003774600.1| guanyl-specific ribonuclease Sa... 250 3e-68 >gi|300310508|ref|YP_003774600.1| guanyl-specific ribonuclease Sa protein [Herbaspirillum seropedicae SmR1] Length = 131 Score = 250 bits (638), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 121/131 (92%), Positives = 124/131 (94%) Query: 3 MRARFISSFFARYWLVLLTALMLSVPVQAKGPVPGNLPEVNLDQLPREAQQTMALIRQGG 62 MRARFI S F+RYWLVLLTALMLS PVQA+GP PG LPEVNLDQLPREAQQTMALIR GG Sbjct: 1 MRARFIFSIFSRYWLVLLTALMLSAPVQARGPAPGGLPEVNLDQLPREAQQTMALIRAGG 60 Query: 63 PFPYEKDGAVFGNYEGVLPKQKRGYYREYTVKTPRARNRGARRIVSGGEPATSGEYYYTS 122 PFPYEKDGAVFGNYEGVLP+QKRGYYREYTVKTPRARNRGARRIVSGGEP TSGEYYYTS Sbjct: 61 PFPYEKDGAVFGNYEGVLPRQKRGYYREYTVKTPRARNRGARRIVSGGEPKTSGEYYYTS 120 Query: 123 DHYKTFRRIRD 133 DHYKTFRRIRD Sbjct: 121 DHYKTFRRIRD 131 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 459,281 Number of extensions: 19038 Number of successful extensions: 40 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 40 Number of HSP's successfully gapped: 1 Length of query: 133 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 58 Effective length of database: 1,264,745 Effective search space: 73355210 Effective search space used: 73355210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 125 (52.8 bits)