BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0180.p (584 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309367|ref|YP_003773459.1| hypothetical protein Hsero_0021... 987 0.0 gi|300309370|ref|YP_003773462.1| hypothetical protein Hsero_0024... 765 0.0 gi|300313607|ref|YP_003777699.1| hypothetical protein Hsero_4322... 263 4e-71 gi|300309372|ref|YP_003773464.1| hypothetical protein Hsero_0026... 208 7e-55 gi|300309373|ref|YP_003773465.1| hypothetical protein Hsero_0027... 92 8e-20 >gi|300309367|ref|YP_003773459.1| hypothetical protein Hsero_0021 [Herbaspirillum seropedicae SmR1] Length = 576 Score = 987 bits (2552), Expect = 0.0, Method: Compositional matrix adjust. Identities = 466/573 (81%), Positives = 512/573 (89%), Gaps = 2/573 (0%) Query: 12 EPTRLVGTAWDEDGNDVAQSVLTGENQKVRALCLTTPEVVVPILFVPGIMGTRLRVSKRD 71 EPTRLVGT WD+DGNDVAQSVLTG+N KVRALCLTTP+VVVPILFVPGIMGTRL+V+ RD Sbjct: 5 EPTRLVGTTWDDDGNDVAQSVLTGQNMKVRALCLTTPDVVVPILFVPGIMGTRLKVTGRD 64 Query: 72 QGPAWLPPENTWETITLGLTHLVRTAADRQRLLNPETTEVDDDGPAFPDDTSKTLLSLAP 131 +GPAW PPE+TWE++ L HLVRT ADRQRLLNP+TTEVD+DGPA PDDTSKTLLSLAP Sbjct: 65 KGPAWFPPESTWESLVLAFKHLVRTPADRQRLLNPDTTEVDEDGPASPDDTSKTLLSLAP 124 Query: 132 GQTDAERIKWRGWGQLHADSYLGILSLLETSMAMIFDPASQGARLTSHWKELVMDRQDAA 191 G TDAERIKWRGWGQLHADSYLGILS+LETSMAMIFDPASQG LTS WKELVMDRQDAA Sbjct: 125 GNTDAERIKWRGWGQLHADSYLGILSMLETSMAMIFDPASQGTLLTSRWKELVMDRQDAA 184 Query: 192 KLGAQKPFVAISDEDLRDAAEVLYPVHAVGYNWLQSNLVSAQRLAEEIERITAYYRSKGK 251 KLGAQKPFV + ++ LRDAAE+LYPVHAVGYNWLQSN VS QRLA EIERITAYYRSKGK Sbjct: 185 KLGAQKPFVPLEEDHLRDAAELLYPVHAVGYNWLQSNKVSGQRLAGEIERITAYYRSKGK 244 Query: 252 RCEGVILITHSMGGLVARACARLPGMTERILGVIHGVMPAIGAPATYKRIRAGFEGMAQV 311 CE VIL+THSMGGLVARACA+LPGM ERILGV+HGVMPAIGAPATYKRIRAGFEGMAQV Sbjct: 245 SCEQVILVTHSMGGLVARACAQLPGMAERILGVVHGVMPAIGAPATYKRIRAGFEGMAQV 304 Query: 312 VLGRDAADCTAVMANAPGPLELLPTAQYKTWTNQGERHWLRASYRAIGQRGMPEEMDSFL 371 VLGR+AADCTAVMANAPGPLELLPTAQYKTWTNQGERHWLR SYRAIGQRGMPEEMDSFL Sbjct: 305 VLGRNAADCTAVMANAPGPLELLPTAQYKTWTNQGERHWLRTSYRAIGQRGMPEEMDSFL 364 Query: 372 GEGDPYAQIYLNNTSDWWKLVREDLIDPAGREDRERAESEGKLLVRTAEETSDFDRYRKK 431 GEGDPYA+IYLNNT DWWKLVRE+LIDPAG+ED+ERA SE K+L R ++SDF+++ K+ Sbjct: 365 GEGDPYAEIYLNNTLDWWKLVREELIDPAGKEDKERARSENKVLAREDAKSSDFEQFAKR 424 Query: 432 MEVAQLFHQHIKDSYHPNTYAYYAADPQQPAWNEINWKCNRMVSGDPLKARLDADDLNGT 491 M+ A HQ I+D YHPNTYAYYAADPQQPAWNEINWKC MV GDP KA+L DDLNG Sbjct: 425 MDKALTLHQQIEDQYHPNTYAYYAADPQQPAWNEINWKCGPMVPGDPAKAQLAEDDLNGM 484 Query: 492 VTLSFDQYHSHNQFFILQRGTGPGDGTVPAESGRSPQPHVVQLFKHEGKLKSHESYDHQF 551 + L F ++ H +FF LQ GTGPGDGTVPAESG +P+PHVVQLFKHEGK KSH+SYDHQF Sbjct: 485 LELRFGEH--HRRFFTLQDGTGPGDGTVPAESGGAPKPHVVQLFKHEGKQKSHDSYDHQF 542 Query: 552 SFNAKVAQATTFYSIIRIVNTSEILKTLSGEKC 584 S+NAK+AQA T YSIIRIV++S KT SG+KC Sbjct: 543 SYNAKIAQAVTLYSIIRIVSSSTHFKTPSGDKC 575 >gi|300309370|ref|YP_003773462.1| hypothetical protein Hsero_0024 [Herbaspirillum seropedicae SmR1] Length = 534 Score = 765 bits (1976), Expect = 0.0, Method: Compositional matrix adjust. Identities = 377/528 (71%), Positives = 433/528 (82%), Gaps = 3/528 (0%) Query: 7 PEQAAEPTRLVGTAWDEDGNDVAQSVLTGENQKVRALCLTTPEVVVPILFVPGIMGTRLR 66 P AA P RLV TAWDE GNDV QSVLTG++ KVRALCLTTP+VV+PI+FVPGIMGTRL+ Sbjct: 4 PAAAAGPMRLVPTAWDEVGNDVMQSVLTGKSMKVRALCLTTPDVVIPIVFVPGIMGTRLK 63 Query: 67 VSKRDQGPAWLPPENTWETITLGLTHLVRTAADRQRLLNPETTEVDDDGPAFPDDTSKTL 126 V R + AW PP+ WE++ L L H+ RTAADRQRLLNP TTEVDDDGPAFPDD SKTL Sbjct: 64 VKGRIKDAAWFPPDGGWESLVLALRHIGRTAADRQRLLNPATTEVDDDGPAFPDDISKTL 123 Query: 127 LSLAPGQTDAERIKWRGWGQLHADSYLGILSLLETSMAMIFDPASQGARLTSHWKELVMD 186 L APG+TDAERIKWRGWGQLHA SYL ILSLLE+SMA+IFDPASQG +LTSHW++LVM Sbjct: 124 LGFAPGKTDAERIKWRGWGQLHASSYLSILSLLESSMALIFDPASQGKKLTSHWQDLVMS 183 Query: 187 RQDAAKLGAQKPFVAISDEDLRDAAEVLYPVHAVGYNWLQSNLVSAQRLAEEIERITAYY 246 RQDAAKLGAQKPF + + L+DAAE LYPVHAVGYNWLQSN VSAQRLA EIERITAYY Sbjct: 184 RQDAAKLGAQKPFKPLQERQLQDAAEFLYPVHAVGYNWLQSNKVSAQRLASEIERITAYY 243 Query: 247 RSKGKRCEGVILITHSMGGLVARACARLPGMTERILGVIHGVMPAIGAPATYKRIRAGFE 306 RSKG+ CE VI++THSMGGLVARACA+LP M ERILGV+HGVMPAIGAPATYKRIRAGFE Sbjct: 244 RSKGRSCEEVIVVTHSMGGLVARACAQLPEMAERILGVVHGVMPAIGAPATYKRIRAGFE 303 Query: 307 GMAQVVLGRDAADCTAVMANAPGPLELLPTAQYKTWTNQGERHWLRASYRAIGQRGMPEE 366 G+ QVVLGR+ A+CTAVMANAPGPLELLPTAQYKTWTNQGERHWLR SY IGQRG+PEE Sbjct: 304 GIGQVVLGRNGAECTAVMANAPGPLELLPTAQYKTWTNQGERHWLRVSYLGIGQRGLPEE 363 Query: 367 MDSFLGEGDPYAQIYLNNTSDWWKLVREDLIDPAGREDRERAESEGKLLVRTAEETSDFD 426 M+SFLGEGDPYA+IYLNNT+DWWKL+REDL++PAGREDRER + L DFD Sbjct: 364 MESFLGEGDPYAKIYLNNTADWWKLIREDLVNPAGREDRERNTRTERALHGNLRR-PDFD 422 Query: 427 RYRKKMEVAQLFHQHIKDSYHPNTYAYYAADPQQPAWNEINWKCNRMVSGDPLKARLDAD 486 RY + ++ A H+ I YHP TYAYYAADPQ+P+WNE+ WKC + D A+ D Sbjct: 423 RYAQNLQKASELHKLIHQRYHPRTYAYYAADPQRPSWNEVQWKCPVSLPDDIKTAKHRDD 482 Query: 487 DLNGTVTLSFDQYHSHNQFFILQRGTGPGDGTVPAESGRSPQPHVVQL 534 +LNG + L+F + + F+LQ +GPGDGTVPAESG +P P+V+Q+ Sbjct: 483 NLNGEIFLAFKKDIARR--FVLQDPSGPGDGTVPAESGAAPTPYVLQI 528 >gi|300313607|ref|YP_003777699.1| hypothetical protein Hsero_4322 [Herbaspirillum seropedicae SmR1] Length = 534 Score = 263 bits (671), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 197/578 (34%), Positives = 291/578 (50%), Gaps = 84/578 (14%) Query: 15 RLVGTAWDEDGNDVAQSVLTGENQKVRALCLTTPEVVVPILFVPGIMGTRLRVSKRDQGP 74 RL+ +D+ GN +S L+ + A+C P+ V+P++FVPG+MG+ L+ G Sbjct: 8 RLLTATFDKKGNPEWKSTLSSPDDSY-AVCHMIPDRVIPVIFVPGVMGSNLKGIGDADGV 66 Query: 75 AW-LPPENTWETITLGLTHLVRTAADRQRLLNPETTEVDDDGPAFPDDTSKTLLSLAPGQ 133 W L E + L L R A R+R L P+ VDDDG P T++ Sbjct: 67 RWRLDSEFSM------LMWLTRGPAKRKRYLTPKKMVVDDDGTR-PSGTAQ--------- 110 Query: 134 TDAERIKWRGWGQLHADSYLGILSLLETSMAMIFDPASQGARLTSHWKELVMDRQDAAKL 193 E +K RGWG++ A SY L LE ++ F G R +L++ R L Sbjct: 111 -HGEELKRRGWGEVGAMSYGDFLVWLENALN-DFTNTKGGHR------DLLIGRT----L 158 Query: 194 GAQKPFVAISDEDLRDAAEVLYPVHAVGYNWLQSNLVSAQRLAEEIERITAYYRSKGKRC 253 G+ K A+ ++ + +PVHA GYNWL SN SA++L E I + A Y+ + KRC Sbjct: 159 GSMKSDEALLKAEVALSYRYRFPVHAFGYNWLDSNDASAKQLRERINSVIARYKKEKKRC 218 Query: 254 EGVILITHSMGGLVARACARLPGMTERILGVIHGVMPAIGAPATYKRIRAGFEG--MAQV 311 E VIL+THSMGGLVAR C+ + GM+++ILG++HGVMPAIGA A Y+R ++G EG +A Sbjct: 219 EKVILVTHSMGGLVARYCSEVLGMSDKILGIVHGVMPAIGAAAVYRRFKSGTEGGWLATK 278 Query: 312 VLGRDAADCTAVMANAPGPLELLPTAQYKTWTNQGERHWLRASYRAIGQRGMPEEMDSFL 371 VLG DAA+ TAV+++APGPL+LLPT +Y WLR + + S Sbjct: 279 VLGEDAAEMTAVLSSAPGPLQLLPTPEYGN-------GWLRIDGGST--------VVSLP 323 Query: 372 GEGDPYAQIYLNNTSDWWKLVREDLIDPAGREDRERAESEGKLLVRTAEETSDF-DRYRK 430 DPY +IY WW + + LI P R A + ++ + +DF D Sbjct: 324 RNNDPYYEIY-TVRGRWWSMCEDHLITP--RTTMLSASQKREI----DADWADFSDVINS 376 Query: 431 KMEVAQLFHQHIKDSYHPNTYAYYAADPQQPAWNEINWKCN---------RMVSGDPLKA 481 K++ FHQ I YHPNTYA++ +D A+ + W+ + D A Sbjct: 377 KVKT---FHQEITGEYHPNTYAFFGSDRHFKAYGTVQWRTTFQSPRRPPIPRPNKDVFDA 433 Query: 482 RLD-ADDLNGTVTLSFDQYHSH-----NQFFILQRGTGPGDGTVPAESGRSPQPHVVQLF 535 +L+ +L T T + S N+ F + GDGTVP SG +P+ H Sbjct: 434 KLNFYGELGETRTALYPHGMSAGQIAPNEVFRISEPDEDGDGTVPHRSGIAPKEHP---- 489 Query: 536 KHEGKLKS--HESYDHQFSF-NAKVAQATTFYSIIRIV 570 +KS S H+ ++ ++ +A+ T +I++IV Sbjct: 490 ----GMKSLLRISVGHESAYKDSDIAREFTLRAIVQIV 523 >gi|300309372|ref|YP_003773464.1| hypothetical protein Hsero_0026 [Herbaspirillum seropedicae SmR1] Length = 161 Score = 208 bits (530), Expect = 7e-55, Method: Compositional matrix adjust. Identities = 95/134 (70%), Positives = 112/134 (83%) Query: 13 PTRLVGTAWDEDGNDVAQSVLTGENQKVRALCLTTPEVVVPILFVPGIMGTRLRVSKRDQ 72 PTRLV TAWDE GNDVAQSVLTG+ KVRALCLT+P++V+P++FVP IMGTRL++ R + Sbjct: 10 PTRLVPTAWDEQGNDVAQSVLTGQRMKVRALCLTSPDIVIPVVFVPSIMGTRLKLKGRGK 69 Query: 73 GPAWLPPENTWETITLGLTHLVRTAADRQRLLNPETTEVDDDGPAFPDDTSKTLLSLAPG 132 GPAW PPE WET+ L L H+ RTAADRQRLLNP+ TE D+DG A+PDD SKTLL+ APG Sbjct: 70 GPAWFPPEGKWETLALALKHVGRTAADRQRLLNPDNTEADEDGSAYPDDISKTLLAFAPG 129 Query: 133 QTDAERIKWRGWGQ 146 +TDAERIKW GWG Sbjct: 130 KTDAERIKWWGWGN 143 >gi|300309373|ref|YP_003773465.1| hypothetical protein Hsero_0027 [Herbaspirillum seropedicae SmR1] Length = 144 Score = 92.4 bits (228), Expect = 8e-20, Method: Compositional matrix adjust. Identities = 41/58 (70%), Positives = 50/58 (86%) Query: 163 MAMIFDPASQGARLTSHWKELVMDRQDAAKLGAQKPFVAISDEDLRDAAEVLYPVHAV 220 MA+IFDPAS+G +LTSHW+ELVM RQDAA+LGAQKPF + ++ L+ AAE LYPVHAV Sbjct: 1 MALIFDPASRGRKLTSHWQELVMARQDAARLGAQKPFTPLQEKQLQQAAEALYPVHAV 58 Score = 87.8 bits (216), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 37/53 (69%), Positives = 47/53 (88%) Query: 524 GRSPQPHVVQLFKHEGKLKSHESYDHQFSFNAKVAQATTFYSIIRIVNTSEIL 576 G +P+PHVVQLFKHEGK K HESYDHQFS+NA ++QA T YSIIRIV++S+++ Sbjct: 83 GAAPKPHVVQLFKHEGKRKGHESYDHQFSYNAPISQAVTLYSIIRIVSSSKLV 135 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,947,098 Number of extensions: 85914 Number of successful extensions: 271 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 262 Number of HSP's successfully gapped: 6 Length of query: 584 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 494 Effective length of database: 1,193,720 Effective search space: 589697680 Effective search space used: 589697680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)