BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0173.p (464 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309362|ref|YP_003773454.1| transmembrane sensory transduct... 736 0.0 gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance ... 268 9e-73 gi|300311800|ref|YP_003775892.1| two component heavy metal senso... 222 6e-59 gi|300312679|ref|YP_003776771.1| periplasmic sensor signal trans... 139 4e-34 gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 127 2e-30 >gi|300309362|ref|YP_003773454.1| transmembrane sensory transduction histidine kinase for cobalt/zinc/ cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 467 Score = 736 bits (1901), Expect = 0.0, Method: Compositional matrix adjust. Identities = 370/454 (81%), Positives = 411/454 (90%) Query: 1 MSLRLVVLFGLLALVLFTAVGSYLSHSLHMQLEARDDEELAGKCRSINHLVEHAASLEDI 60 +SLRLV LFGLLA+V+ A+G+YLSHSLH+QLE RDDEELAGKCRSINHLVEHAASLEDI Sbjct: 9 LSLRLVALFGLLAMVILAALGTYLSHSLHVQLERRDDEELAGKCRSINHLVEHAASLEDI 68 Query: 61 LARRHLVLDTLSGHDQLLVRIARADGSTLLESHDPTRTSPDVDLSTVKIRSWDHIEALRM 120 LARRHLVLDTL+GHDQLLVRIARADGSTLLESHDPTRT P +DLS V IR+WD I++ ++ Sbjct: 69 LARRHLVLDTLTGHDQLLVRIARADGSTLLESHDPTRTPPGIDLSQVDIRAWDSIQSWKL 128 Query: 121 GDLRARYIASMPVTRSGEKAIVLVVRTASDRMLLLADYRADVWWAAAIGTVLATLLSYFL 180 GDLRARY+AS PVTRSGE+A+VLVVRTASDRMLLLA+YR+DVWWAA +G +LA L+SYFL Sbjct: 129 GDLRARYVASKPVTRSGEQAVVLVVRTASDRMLLLAEYRSDVWWAAGVGALLAILMSYFL 188 Query: 181 VRSGLRPLRMMAAQTRAISANKLDTRLDLQAAPRELHEIVQSFNDMLDRLHRSFQQLSQF 240 VRSGLRPLR MAAQTRA+SAN+LDTRLDLQAAPRELHEIV+SFN+MLDRLHRSFQQLSQF Sbjct: 189 VRSGLRPLRTMAAQTRAVSANRLDTRLDLQAAPRELHEIVESFNEMLDRLHRSFQQLSQF 248 Query: 241 SADLAHDVRTPLNNLMVQTQVALGKPRSLDEYQALLSSNIEEYERLSRMMESMLFLARAD 300 SADLAHDVRTPLNNLMVQTQVALGKPR +DEYQALLSSNIEEYERLSRMMESMLFLARA+ Sbjct: 249 SADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQALLSSNIEEYERLSRMMESMLFLARAE 308 Query: 301 NAHVVLQIQPLDVAEELNRIADYFEGIAEEAQVRIAVAGSGTLYADATLLRRAVGNLVAN 360 NAHVVLQ Q LDVA+EL RIADYFEGIA+EAQVR+ V G+G L ADATLLRRAVGNLVAN Sbjct: 309 NAHVVLQWQELDVAQELTRIADYFEGIADEAQVRLVVQGAGRLQADATLLRRAVGNLVAN 368 Query: 361 AIRYTPPQGIISLEATVVAGATLIGVANPGPGIPPDQIERIFDRFYRADPSRSSASGSSG 420 AIRYTP G+I L+ I V+NPG GIP +Q+ER+FDRFYRADPSRSSA GSSG Sbjct: 369 AIRYTPADGVIRLQVDAAGDGMEISVSNPGAGIPAEQLERLFDRFYRADPSRSSAGGSSG 428 Query: 421 LGLAIVRTIMALHGGQASASSEPGRRTVFTLRFP 454 LGLAIVR+IM LHGGQAS +S PG+ TVF+LRFP Sbjct: 429 LGLAIVRSIMMLHGGQASVTSSPGQLTVFSLRFP 462 >gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 268 bits (684), Expect = 9e-73, Method: Compositional matrix adjust. Identities = 175/466 (37%), Positives = 264/466 (56%), Gaps = 16/466 (3%) Query: 1 MSLRLVVLFGLLALVLFTAVGSYLSHSLHMQLEARDDEELAGKCRSINHLVEHAASLEDI 60 ++ R+ LF L+ ++ T +G YL S L R D+ L G+ +L+ ++E Sbjct: 13 LTTRVTALFALMTSLVVTGLGFYLDRSAGQALSQRADQALIGRVEHFRNLMHDLYNVEQ- 71 Query: 61 LARRHLVLDTLSGHDQLLVRIARADGSTLLESHDPTRTSPDV--------DLSTVKIRSW 112 + +R + +++ G++Q VRI R G +P P +S+ +R+ Sbjct: 72 MEQRPALFESMMGNEQD-VRIFRRQGEAPFIQSNPDHLVPPAMTPLPVGSPVSSASLRT- 129 Query: 113 DHIEALRMGDLRARYIASMP-VTRSGEKAIVLVVRTASDRMLLLADYRADVWWAAAIGTV 171 + R +R R+++++ V + G+ ++ ++ YR + AAA V Sbjct: 130 ----SQRADGVRVRWVSALADVGKGGDTVEIVAAYVMVQEARMMRSYRWRIAGAAAAAVV 185 Query: 172 LATLLSYFLVRSGLRPLRMMAAQTRAISANKLDTRLDLQAAPRELHEIVQSFNDMLDRLH 231 L LL + L+R GL+PL M+ + I+ + L RLD + P EL + SFN MLDRL Sbjct: 186 LTALLGFLLLRRGLQPLEAMSQRAAQITPDHLSARLDQEGMPAELRRVALSFNAMLDRLE 245 Query: 232 RSFQQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRSLDEYQALLSSNIEEYERLSRMME 291 + L+QFS DLAH++RTP+N LM Q+QVALG+ RS +EY+ +L SN+EE +RLSR++E Sbjct: 246 AGYAHLAQFSGDLAHEIRTPINVLMGQSQVALGQRRSPEEYEQVLESNVEELQRLSRIVE 305 Query: 292 SMLFLARADNAHVVLQIQPLDVAEELNRIADYFEGIAEEAQVRIAVAGSGTLYADATLLR 351 ++LFLA+AD+A + ++ LD+AEEL RI DYFEGIA+E +R V +G A+ + R Sbjct: 306 NILFLAQADHAALAVERNALDLAEELGRITDYFEGIADERGMRFDVQAAGQCQANLIMWR 365 Query: 352 RAVGNLVANAIRYTPPQGIISLEATVVAGATLIGVANPGPGIPPDQIERIFDRFYRADPS 411 R V NLV NA+RY + I + A + I V N + + R+FDRFYR D S Sbjct: 366 RVVSNLVINAVRYGRAESTIRILAESDGEGSRIMVENRSEDVTAQAMARMFDRFYRGDRS 425 Query: 412 RSSASGSSGLGLAIVRTIMALHGGQASASSEPGRRTVFTLRFPEAA 457 RS+ + S+GLGLAIV+ IMALHGG A+ F+LRFP AA Sbjct: 426 RSAFTESNGLGLAIVKAIMALHGGTAAVECPAPGWIRFSLRFPSAA 471 >gi|300311800|ref|YP_003775892.1| two component heavy metal sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 467 Score = 222 bits (565), Expect = 6e-59, Method: Compositional matrix adjust. Identities = 115/289 (39%), Positives = 183/289 (63%), Gaps = 5/289 (1%) Query: 168 IGTVLATLLSYFLVRSGLRPLRMMAAQTRAISANKLDTRLDLQAAPRELHEIVQSFNDML 227 +G VLA + SY++ R+GL PLR M+ + ++ S KL R+ ++A P+EL + +FN L Sbjct: 176 LGIVLAVISSYWIARAGLAPLRQMSDRAQSFSPRKLSARMPVEAMPQELSVLALAFNGAL 235 Query: 228 DRLHRSFQQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRSLDEYQALLSSNIEEYERLS 287 DR+ ++ QL F+AD+AH++RTPL NL+ +TQVAL + RS E++ +L SN+EE +R+ Sbjct: 236 DRIEDAYTQLEAFNADVAHELRTPLANLIGETQVALARERSAGEFKDVLQSNLEELDRIR 295 Query: 288 RMMESMLFLARADNAHVVLQIQPLDVAEELNRIADYFEGIAEEAQVRIAVAG--SGTLYA 345 ++ MLFLAR+D P VA+E+ + D+FE + +++ +RI VAG T Sbjct: 296 SIVNDMLFLARSDQGEAATARVPARVADEVQKTVDFFEFVLDDSGMRIEVAGDTEATAMM 355 Query: 346 DATLLRRAVGNLVANAIRYTPPQGIISLEATVVAGATLIGVANPGPGIPPDQIERIFDRF 405 + L +RA+ NL+ NAI+++P ++ ++ + V+NPG I + R+FDRF Sbjct: 356 ETALFKRALTNLIQNAIQHSPEGALLRIDIACQEETVRVAVSNPGKPIDEHHLPRLFDRF 415 Query: 406 YRADPSRSSASGS--SGLGLAIVRTIMALHGGQASASSEPGRRTV-FTL 451 YR D +R +GS GLGLAIV+ + +HGG+A ASS+ G ++ FT+ Sbjct: 416 YRVDSARQDINGSHGHGLGLAIVKAVARMHGGRAFASSKEGVNSIGFTI 464 >gi|300312679|ref|YP_003776771.1| periplasmic sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 139 bits (350), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 96/281 (34%), Positives = 145/281 (51%), Gaps = 12/281 (4%) Query: 181 VRSGLRPLRMMAAQTRAISANKLDTRLDLQAAPRELHEIVQSFNDMLDRLHRSFQQLSQF 240 VR GLRPL+ + KL +D + AP EL ++ + N+++ R+++ Q F Sbjct: 182 VRRGLRPLKSLQRNLEQRDHGKLGA-IDGRGAPVELQPLIVAMNELMARINKGEQAQQNF 240 Query: 241 SADLAHDVRTPLNNLMVQTQVALGKPRSLDEYQALLSSNIEEYERLSRMMESMLFLARAD 300 AD+AH +RTPL L +Q ++ K + + L+ ER+ R +L LAR++ Sbjct: 241 MADVAHQLRTPLTGLKLQLELLHDKHQDQPDTARSLAMMNSSVERMIRQSRQLLALARSE 300 Query: 301 NA-HVVLQIQPLDVAEELNRIADYFEGIAEEAQVRIAVAGS---GTLYADATLLRRAVGN 356 +++ L + + + +F I E + RI + L D LL + N Sbjct: 301 PGLFESRKLEELSLDKLVEESVQHF--IEEADKKRIDLGFDLRPARLRGDRFLLSDLIDN 358 Query: 357 LVANAIRYTPPQGIISLEATVVAGATLIGVANPGPGIPPDQIERIFDRFYRADPSRSSAS 416 L+ NA+RY+PP G +++ AT++ V + GPGI P+ E IFDRFYRA+ Sbjct: 359 LIDNAVRYSPPHGTVTVRCLEEQDATVLSVEDSGPGIAPEHRELIFDRFYRAN----DKV 414 Query: 417 GSSGLGLAIVRTIMALHGGQASA-SSEPGRRTVFTLRFPEA 456 SGLGLAIVR I HGG S E G+ T+FT+RFP A Sbjct: 415 AGSGLGLAIVREIAHDHGGVISVDCKEQGQGTIFTVRFPLA 455 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 127 bits (318), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 92/272 (33%), Positives = 140/272 (51%), Gaps = 19/272 (6%) Query: 175 LLSYFLVRSGLRPLRMMAAQTRAISANKLDTRLDLQAAPRELHEIVQSFNDMLDRLHRSF 234 +LS+ V GL P+R AA+ A N L D+ P+E+ + + ND+L RL + Sbjct: 155 VLSWVAVSRGLAPVRKAAAEVAARDMNSLQPLADV-GMPQEIQPLTHALNDLLARLKHAT 213 Query: 235 QQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRSLDEYQ--ALLSSNIEEYERLSRMMES 292 + F AD AH++RTPL L +QTQ+A R++DE + A + ER + ++ Sbjct: 214 ETQRAFVADAAHELRTPLTALRLQTQLA---ERAVDEEERRAAFADLKNGLERTTHLVHQ 270 Query: 293 MLFLARADNAHVVLQIQPLDVAEELNRIADYFEGIAEEAQVRIAVAGSGT------LYAD 346 +L LAR + + +D+ L + +AE + + ++ G + + Sbjct: 271 LLTLARQEPD--AMSFTQVDLRALLGSVVGDLSSVAEHGGIDLGLSDQGDDGRPLRVTGN 328 Query: 347 ATLLRRAVGNLVANAIRYTPPQGIISLEATV-VAGATLIGVANPGPGIPPDQIERIFDRF 405 A LR NLV NA+RYTP G++ + + AG + V + GPGI ++ R+FDRF Sbjct: 329 ADALRILFNNLVDNALRYTPAGGVVDVGVDLSTAGYCTVVVQDSGPGIAESELPRVFDRF 388 Query: 406 YRADPSR----SSASGSSGLGLAIVRTIMALH 433 YRA S+ S A+ SGLGLAIVR I LH Sbjct: 389 YRAQHSQEGSGSRAAFGSGLGLAIVRQIADLH 420 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,298,343 Number of extensions: 50912 Number of successful extensions: 346 Number of sequences better than 1.0e-08: 40 Number of HSP's gapped: 294 Number of HSP's successfully gapped: 40 Length of query: 464 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 375 Effective length of database: 1,198,455 Effective search space: 449420625 Effective search space used: 449420625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)