BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0172.p (223 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309361|ref|YP_003773453.1| response regulator for cobalt/z... 425 e-120 gi|300312356|ref|YP_003776448.1| cobalt/zinc/cadmium resistance ... 294 4e-81 gi|300311799|ref|YP_003775891.1| two component heavy metal respo... 273 1e-74 gi|300313680|ref|YP_003777772.1| two component response regulato... 173 8e-45 gi|300313896|ref|YP_003777988.1| two component transcription res... 159 2e-40 >gi|300309361|ref|YP_003773453.1| response regulator for cobalt/zinc/cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 235 Score = 425 bits (1092), Expect = e-120, Method: Compositional matrix adjust. Identities = 211/219 (96%), Positives = 218/219 (99%) Query: 1 MHILLVEDEPKSGEYLRRGLTESGFVVDWVQTGVDGLHNARTLDYKLIILDVMLPGMNGW 60 MHILLVEDEPKSG+YLRRGLTESGFVVDWVQTGVDGLHNARTLDYKLIILDVMLPGMNGW Sbjct: 1 MHILLVEDEPKSGDYLRRGLTESGFVVDWVQTGVDGLHNARTLDYKLIILDVMLPGMNGW 60 Query: 61 DILRELRRTHDTPVLFLTARDEVEDRVKGLELGADDYLVKPFAFTELLARVRTLLRRGPS 120 DILRELRRTHDTPVLFLTARDEVEDRVKGLELGADDYLVKPFA+TELLARVRTLLRRGPS Sbjct: 61 DILRELRRTHDTPVLFLTARDEVEDRVKGLELGADDYLVKPFAYTELLARVRTLLRRGPS 120 Query: 121 READLIEYEDVVVDVIRRKVTRAGQRIDLTAKEFALLHMLIRRPGEVLSRPQIASQVWDM 180 RE DLIEY+DVV+DV+RRKVTRAGQRIDLTAKEFALLHMLIRRPGEVLSRPQIASQVWDM Sbjct: 121 REVDLIEYDDVVLDVLRRKVTRAGQRIDLTAKEFALLHMLIRRPGEVLSRPQIASQVWDM 180 Query: 181 NFDSDTNVVDVAVRRLRAKLDEGFDQKLLHTVWGMGYVF 219 NFDSDTNVVDVAVRRLRAKLDEGF++KLLHTVWGMGYVF Sbjct: 181 NFDSDTNVVDVAVRRLRAKLDEGFEKKLLHTVWGMGYVF 219 >gi|300312356|ref|YP_003776448.1| cobalt/zinc/cadmium resistance two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 223 Score = 294 bits (752), Expect = 4e-81, Method: Compositional matrix adjust. Identities = 140/220 (63%), Positives = 177/220 (80%) Query: 1 MHILLVEDEPKSGEYLRRGLTESGFVVDWVQTGVDGLHNARTLDYKLIILDVMLPGMNGW 60 M IL++ED+PK+G+YLR+GL ESG+ VD + G DGLH A DY L++LDVMLPG++GW Sbjct: 1 MAILVIEDDPKTGDYLRKGLRESGYAVDLARNGADGLHMALEQDYDLVVLDVMLPGLDGW 60 Query: 61 DILRELRRTHDTPVLFLTARDEVEDRVKGLELGADDYLVKPFAFTELLARVRTLLRRGPS 120 ++ LR D PVLFLTARD V+DR++GL+LGADDYLVKPF+FTEL+ R+RTLLRRG + Sbjct: 61 QVMSALRSKRDLPVLFLTARDHVDDRIRGLQLGADDYLVKPFSFTELVLRIRTLLRRGVT 120 Query: 121 READLIEYEDVVVDVIRRKVTRAGQRIDLTAKEFALLHMLIRRPGEVLSRPQIASQVWDM 180 REA++ E D+ +D +R KVTR G I LT KEF LLH+LI+R GE LSR IASQ+WDM Sbjct: 121 REAEVYEIADLQLDHVRHKVTRQGVNIALTNKEFMLLHLLIKRQGEALSRSVIASQIWDM 180 Query: 181 NFDSDTNVVDVAVRRLRAKLDEGFDQKLLHTVWGMGYVFE 220 NFDSDTNVVDVA++RLRAK+D FD+KL+HTV +GY+F Sbjct: 181 NFDSDTNVVDVAIKRLRAKIDAPFDKKLIHTVRSVGYMFS 220 >gi|300311799|ref|YP_003775891.1| two component heavy metal response transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 230 Score = 273 bits (697), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 135/222 (60%), Positives = 168/222 (75%) Query: 1 MHILLVEDEPKSGEYLRRGLTESGFVVDWVQTGVDGLHNARTLDYKLIILDVMLPGMNGW 60 M IL+VEDE K EYL +GLTE G VD GVDG H A DY +I+LDVMLPG++G+ Sbjct: 1 MKILIVEDEQKMSEYLCKGLTEQGCAVDLAANGVDGQHLAIEHDYDVIVLDVMLPGLDGF 60 Query: 61 DILRELRRTHDTPVLFLTARDEVEDRVKGLELGADDYLVKPFAFTELLARVRTLLRRGPS 120 +LR LR+ +TPV+ LTARD VEDR+KGL GADDYL+KPF+F ELLAR++ L RRG + Sbjct: 61 SVLRALRKVKETPVIMLTARDSVEDRIKGLHDGADDYLIKPFSFLELLARLQALTRRGRA 120 Query: 121 READLIEYEDVVVDVIRRKVTRAGQRIDLTAKEFALLHMLIRRPGEVLSRPQIASQVWDM 180 +E + D+ +D++ RK R G RIDLTAKEFALL ++ RR GE+LS+ IA VWDM Sbjct: 121 QEPAQLRIGDLQIDLLSRKAFRGGLRIDLTAKEFALLAVMARRQGEILSKTAIAELVWDM 180 Query: 181 NFDSDTNVVDVAVRRLRAKLDEGFDQKLLHTVWGMGYVFEVR 222 NFDS+TNVV+VA++RLRAK+D F+QKLLHTV GMGYV E R Sbjct: 181 NFDSNTNVVEVAIKRLRAKIDAPFEQKLLHTVRGMGYVLEPR 222 >gi|300313680|ref|YP_003777772.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 218 Score = 173 bits (439), Expect = 8e-45, Method: Compositional matrix adjust. Identities = 94/219 (42%), Positives = 137/219 (62%), Gaps = 5/219 (2%) Query: 1 MHILLVEDEPKSGEYLRRGLTESGFVVDWVQTGVDGLHNARTLDYKLIILDVMLPGMNGW 60 M +LLVED+P GE +R+GL + GF +DWVQ G RT DY +++LD+ LP +G Sbjct: 1 MRVLLVEDDPMVGEAVRKGLRQDGFAIDWVQDGKSADVALRTEDYAMLLLDLGLPQKDGL 60 Query: 61 DILRELR-RTHDTPVLFLTARDEVEDRVKGLELGADDYLVKPFAFTELLARVRTLLRRGP 119 +LR LR R + PVL TARD V DRV GL+ GADDYL+KPF EL AR+R L RR Sbjct: 61 AVLRTLRERGNAIPVLITTARDAVADRVAGLDAGADDYLIKPFDLEELSARMRALSRRQA 120 Query: 120 SREADLIEYEDVVVDVIRRKVTRAGQRIDLTAKEFALLHMLIRRPGEVLSRPQIASQVWD 179 R L++ +V+++ +VT +G+ ++L+A+EF LL + RPG VLSR Q+ +++ Sbjct: 121 GRAESLVQVREVILNPATHEVTVSGKPVNLSAREFTLLQAFMDRPGVVLSRAQLEEKLYG 180 Query: 180 MNFDSDTNVVDVAVRRLRAKLDEGFDQKLLHTVWGMGYV 218 + ++N V+V + LR K+ F + V G+GY+ Sbjct: 181 WDDSIESNAVEVHIHALRKKVGSDF----IKNVRGVGYL 215 >gi|300313896|ref|YP_003777988.1| two component transcription response regulator protein [Herbaspirillum seropedicae SmR1] Length = 244 Score = 159 bits (402), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 92/224 (41%), Positives = 131/224 (58%), Gaps = 5/224 (2%) Query: 3 ILLVEDEPKSGEYLRRGLTESGFVVDWVQTGVDGLHNARTLDYKLIILDVMLPGMNGWDI 62 IL+VEDEPK E L++ L +G+ V G L R LI+LD+MLPGM+GW+I Sbjct: 18 ILIVEDEPKLAELLQKYLALAGYSARHVARGDAALDAVRAQRPDLILLDIMLPGMDGWEI 77 Query: 63 LRELRRTHDTPVLFLTARDEVEDRVKGLELGADDYLVK-PFAFTELLARVRTLLRRGP-- 119 R+LR D PVL LTAR E EDR++GLELGADDY+ K PF+ E++ARV+++LRR Sbjct: 78 CRQLRTFSDVPVLMLTARAEEEDRLRGLELGADDYIGKTPFSPREIVARVKSMLRRNALV 137 Query: 120 -SREADLIEYEDVVVDVIRRKVTRAGQRIDLTAKEFALLHMLIRRPGEVLSRPQIASQVW 178 R A + +D +R + + G+ ++LT E LL PG+V SR Q+ + + Sbjct: 138 QQRTAQAPARSLLHIDDVRHQASVRGEALNLTPLELRLLKTFASAPGQVFSREQLLNHLH 197 Query: 179 DMNFDSDTNVVDVAVRRLRAKLDEGFD-QKLLHTVWGMGYVFEV 221 D + VD ++ LR KL+ F + V+G+GY FE+ Sbjct: 198 DDDRSVTDRAVDTHIKNLRRKLEPCFPGHNAIQAVYGVGYCFEL 241 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.142 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 711,842 Number of extensions: 29315 Number of successful extensions: 213 Number of sequences better than 1.0e-08: 38 Number of HSP's gapped: 194 Number of HSP's successfully gapped: 38 Length of query: 223 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 141 Effective length of database: 1,231,600 Effective search space: 173655600 Effective search space used: 173655600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 129 (54.3 bits)