BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0163.p (254 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 498 e-142 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 128 4e-31 gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogena... 127 6e-31 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 117 6e-28 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 117 7e-28 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 498 bits (1282), Expect = e-142, Method: Compositional matrix adjust. Identities = 245/254 (96%), Positives = 250/254 (98%) Query: 1 MSASTGRLAGKTVLITAAAQGIGRASTELFAREGARVIATDISKTHLEELASIAGVETHL 60 MSASTGRLAGKTVLITAAAQGIGRASTELFAREGARVIATDISK HL+ELA IAGVETHL Sbjct: 1 MSASTGRLAGKTVLITAAAQGIGRASTELFAREGARVIATDISKPHLDELAGIAGVETHL 60 Query: 61 LDVTDDDAIKALVAKVGTVDVLFNCAGYVAAGNILECDDKAWDFSFNLNAKAMFHTIRAV 120 LDVTDD AIKALVAK+GT+DVLFNCAGYVAAGNILECDDKAWDFSFNLNAKAMFHTIRAV Sbjct: 61 LDVTDDAAIKALVAKIGTIDVLFNCAGYVAAGNILECDDKAWDFSFNLNAKAMFHTIRAV 120 Query: 121 LPGMLAKKAGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVSQGIRCNAIC 180 LPGMLAKKAGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFV+QGIRCNAIC Sbjct: 121 LPGMLAKKAGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVAQGIRCNAIC 180 Query: 181 PGTIESPSLNQRISTQAKETGKSEDEVRAAFVARQPMGRIGKAEEVAALALYLASDESNF 240 PGTIESPSLNQRISTQAKETGKSE+EVRAAFV RQPMGRIGKAEEVAALALYLASDESNF Sbjct: 181 PGTIESPSLNQRISTQAKETGKSEEEVRAAFVGRQPMGRIGKAEEVAALALYLASDESNF 240 Query: 241 TTGSIHMIDGGWSN 254 TTGSIHMIDGGWSN Sbjct: 241 TTGSIHMIDGGWSN 254 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 128 bits (322), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 90/254 (35%), Positives = 132/254 (51%), Gaps = 23/254 (9%) Query: 8 LAGKTVLITAAAQGIGRASTELFAREGARVIATDISKTHLEELASIAGVETHLLDV---- 63 L GK LIT A+ GIGRA+ LFAR GA +I T + LE++A++ + V Sbjct: 4 LQGKVALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRASVVAGD 63 Query: 64 -----TDDDAIKALVAKVGTVDVLFNCAGYVAAGNIL-ECDDKAWDFSFNLNAKAMFHTI 117 T + + + + G +D+ N AG V A L E + W + + N + F Sbjct: 64 VGEAATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTSAFLGA 123 Query: 118 RAVLPGMLAKKAGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVSQGIRCN 177 R +P MLA+ AG+IV +S S G+ AYGA+KA ++GL K +AAD+ QGIR N Sbjct: 124 RCQIPAMLARGAGAIVFTSSFVGSSAGLPGMAAYGAAKAGLMGLVKGIAADYAVQGIRAN 183 Query: 178 AICPGTIESPSLNQRISTQAKETGKSEDEVRAAFVARQPMGRIGKAEEVAALALYLASDE 237 A+ PG +++ G + + AA + M RI + EE+AA AL+LAS Sbjct: 184 ALLPGGVDT-----------DMGGDPQQKQWAAGL--HAMKRIAQPEEIAAAALFLASPM 230 Query: 238 SNFTTGSIHMIDGG 251 ++F TG+ DGG Sbjct: 231 ASFVTGTALYADGG 244 >gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 266 Score = 127 bits (320), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 88/261 (33%), Positives = 142/261 (54%), Gaps = 19/261 (7%) Query: 8 LAGKTVLITAAAQ-----GIGRASTELFAREGARVIATDIS-----KTHLEELASIAGV- 56 + GK +++T A G G+A+ L+AREG RV+A D S +T + + S GV Sbjct: 5 MQGKAIIVTGAGSVGEGWGNGKAAAVLYAREGGRVLAVDRSIEAALQTQ-DIIRSEGGVC 63 Query: 57 ETHLLDVTDDDAIKAL----VAKVGTVDVLFNCAGYVAAGNILECDDKAWDFSFNLNAKA 112 E DV+ + +KA+ + G VDVL N G G +E +++W+ +N + Sbjct: 64 EVLAADVSRNTEVKAIAEAAMDHFGRVDVLHNNVGIADVGGPVETSEESWNRLVAVNQTS 123 Query: 113 MFHTIRAVLPGMLAKKAGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVSQ 172 +F T + VLP M ++ G+IVNI+S A+ Y A+KAA++ TK+VA + Sbjct: 124 LFLTHKHVLPIMEKQRKGAIVNISSLAALRWIGFPYLGYTATKAAILAFTKNVAMQYAPM 183 Query: 173 GIRCNAICPGTIESPSLNQRISTQAKETGKSEDEVRAAFVARQPMGRIGKAEEVAALALY 232 GIR N + PG +++P + + + G +++RA A+ PMG +G A +VA +L+ Sbjct: 184 GIRANCVLPGMMDTPMIREPLKA---SYGGDIEQMRAKRNAQCPMGFMGDAWDVAHASLF 240 Query: 233 LASDESNFTTGSIHMIDGGWS 253 LASD + + TG ++DGG S Sbjct: 241 LASDHARYITGLDMIVDGGLS 261 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 117 bits (294), Expect = 6e-28, Method: Compositional matrix adjust. Identities = 93/257 (36%), Positives = 131/257 (50%), Gaps = 24/257 (9%) Query: 1 MSASTGRLAGKTVLITAAAQGIGRASTELFAREGARVIATDISKTHLEELASIAGVETHL 60 MS L GKT L+T QGIG A GARVIA + EE A G E L Sbjct: 1 MSFIPDLLKGKTALVTGGTQGIGAAVARQLVALGARVIAAGLPPFAHEEFA---GSEVTL 57 Query: 61 --LDVTDDDAIKALVAKVGTVDVLFNCAGYVAAGNILECDDKAWDFSFNLNAKAMFHTIR 118 ++V +++++A +A + +D++ NCAG + G LE + + NL T+R Sbjct: 58 VEMNVASEESVRAALAGLTQLDIVVNCAGIIKRGAELETEVFEQVIAVNLTG-----TMR 112 Query: 119 --AVLPGMLAKKAGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVSQGIRC 176 A +L + G IVN AS S G Y ASK V LTKS+A + + GIR Sbjct: 113 VCAAARELLRQSRGCIVNTASMLSFFGG-GLVPGYSASKGGVAQLTKSLAIAYAADGIRV 171 Query: 177 NAICPGTIESPSLNQRISTQAKETGKSEDEVRA-AFVARQPMGRIGKAEEVAALALYLAS 235 NA+ PG I +P TQA + +D VRA +AR P+GR G+ E+V + +L S Sbjct: 172 NAVAPGWIATP------LTQALQ----DDPVRAGPILARTPLGRWGQPEDVGQVVAFLCS 221 Query: 236 DESNFTTGSIHMIDGGW 252 ++F TG+I +DGG+ Sbjct: 222 PAASFMTGAIVPVDGGY 238 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 117 bits (294), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 88/265 (33%), Positives = 137/265 (51%), Gaps = 32/265 (12%) Query: 1 MSASTGRLAGKTVLITAAAQGIGRASTELFAREGARVIATDISKT-------HLEELASI 53 MSA L G+ L+T A++GIGRA AR+GA+VI T S++ +L L Sbjct: 1 MSAQN--LQGQVALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPE 58 Query: 54 AGVETHLLDVTDDDAIKALVAKV----GTVDVLFNCAGYVAAGNILECDDKAWDFSFNLN 109 AG +L+V D +A ALV +V G++ +L N AG + D+ WD N Sbjct: 59 AG-RGAVLNVNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATN 117 Query: 110 AKAMFHTIRAVLPGMLAKKAGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADF 169 A+ RAVL GM+ + G I+NI S G + Y A+KA V G+++++A + Sbjct: 118 LTAVGRLSRAVLRGMMKARTGRIINITSVVGEA-GNPGQMNYAAAKAGVAGMSRALAREI 176 Query: 170 VSQGIRCNAICPGTIESP---SLNQRISTQAKETGKSEDEVRAAFVARQPMGRIGKAEEV 226 S+ I N + PG I++ +LN++ S AA + + P+GR+G AEE+ Sbjct: 177 GSRNITVNCVAPGFIDTDMTRALNEQQS--------------AAILQQIPLGRLGAAEEI 222 Query: 227 AALALYLASDESNFTTGSIHMIDGG 251 A +LAS ++ + TG+ ++GG Sbjct: 223 AYAVAFLASPQAAYITGTTLNVNGG 247 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.129 0.359 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 677,453 Number of extensions: 24289 Number of successful extensions: 253 Number of sequences better than 1.0e-08: 49 Number of HSP's gapped: 162 Number of HSP's successfully gapped: 49 Length of query: 254 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 171 Effective length of database: 1,226,865 Effective search space: 209793915 Effective search space used: 209793915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)