BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0153.p (314 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313164|ref|YP_003777256.1| LysR family transcription regul... 594 e-171 gi|300309487|ref|YP_003773579.1| LysR family transcription regul... 264 6e-72 gi|300310701|ref|YP_003774793.1| LysR family transcription regul... 253 2e-68 gi|300310375|ref|YP_003774467.1| LysR family transcription regul... 172 3e-44 gi|300313740|ref|YP_003777832.1| LysR family transcription regul... 86 4e-18 >gi|300313164|ref|YP_003777256.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 324 Score = 594 bits (1532), Expect = e-171, Method: Compositional matrix adjust. Identities = 298/327 (91%), Positives = 305/327 (93%), Gaps = 16/327 (4%) Query: 1 MSNELNSSGDNRLGEEGAAGDALPSVGSIVSRLRFRQVALLTALDEQGSLHKAAEVMHMT 60 MSNELNSSGDNRLGE GA+ LPSVGSIVSRLRFRQVALLTALDEQGSLHKAAEVMHMT Sbjct: 1 MSNELNSSGDNRLGE-GAS--ELPSVGSIVSRLRFRQVALLTALDEQGSLHKAAEVMHMT 57 Query: 61 QPAATKALHEMEDALGVTLFDRSPRGIEATELGRCVIRYARLKKK-------------KN 107 QPAATKALHEMEDALGVTLFDRSPRGIEATELGRCVIRYARL + Sbjct: 58 QPAATKALHEMEDALGVTLFDRSPRGIEATELGRCVIRYARLIQSDVANLRDELQNMISG 117 Query: 108 PGGRLSIGTIMGAAPFVTRALSRLREIQPDVSIEISEDTSARQLLLLDQGRIDLMIGRSS 167 GGRL+IGTIMGAAPFVTRALSRLREIQP+VSIEISEDTSARQLLLLDQGRIDLMIGRSS Sbjct: 118 RGGRLAIGTIMGAAPFVTRALSRLREIQPEVSIEISEDTSARQLLLLDQGRIDLMIGRSS 177 Query: 168 VSSQPNLYHYEMLRGEPMCIVSGIDHPLATAQKVRLQELADASWILYSSNMPMRIWIEHE 227 VSSQPNLYHYEMLRGEPMCIVSGIDHPLATAQKVRLQELA+ASWILYSSNMPMRIWIEHE Sbjct: 178 VSSQPNLYHYEMLRGEPMCIVSGIDHPLATAQKVRLQELAEASWILYSSNMPMRIWIEHE 237 Query: 228 FKLEGVKVPGNVIETASPFVTITLLAQSNMVAVMPLDIAHFFAARQMLGILPVNLKTRIE 287 FKLEGVKVPGNVIETASPFVTITLLAQSNMVAVMPLDIAHFFAA+QMLGILPVNLKTRIE Sbjct: 238 FKLEGVKVPGNVIETASPFVTITLLAQSNMVAVMPLDIAHFFAAKQMLGILPVNLKTRIE 297 Query: 288 HYGIVTRKNSTLSSLAKMFIQILRQQN 314 HYGIVTRKN+TLSSLAKMFIQILRQQN Sbjct: 298 HYGIVTRKNTTLSSLAKMFIQILRQQN 324 >gi|300309487|ref|YP_003773579.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 314 Score = 264 bits (675), Expect = 6e-72, Method: Compositional matrix adjust. Identities = 145/301 (48%), Positives = 204/301 (67%), Gaps = 14/301 (4%) Query: 21 DALPSVGSIVSRLRFRQVALLTALDEQGSLHKAAEVMHMTQPAATKALHEMEDALGVTLF 80 + LPS+ SI SRL RQ+ LL AL + GSL KAAE + +TQP A+KAL E+E LGV LF Sbjct: 3 NTLPSLASITSRLHLRQLRLLIALSDHGSLLKAAEQVALTQPGASKALQEIESTLGVQLF 62 Query: 81 DRSPRGIEATELGRCVIRYARLKKK-------------KNPGGRLSIGTIMGAAPFVTRA 127 R+ RG+E TELG CVIRYARL + + GGRL++GTIMGA P +T A Sbjct: 63 TRTNRGLEPTELGHCVIRYARLIQTDLTHLREEMLAIMQGHGGRLAVGTIMGAVPLLTDA 122 Query: 128 LSRLREIQPDVSIEISEDTSARQLLLLDQGRIDLMIGRSSVSSQPNLYHYEMLRGEPMCI 187 L+RL E P +S+EI EDTSAR L LLDQGR+DL I R+S+S +P+LY + E + + Sbjct: 123 LTRLLEAHPALSVEIVEDTSARLLRLLDQGRLDLAICRTSISQRPDLYDAFNIHEEELAV 182 Query: 188 VSGIDHPLATAQKVRLQELADASWILYSSNMPMRIWIEHEFKLEGVKVPGNVIETASPFV 247 V+ + HPLA A ++L +LAD+ W++YS+NMPMR+ +E EF ++ P +++ET S F Sbjct: 183 VASVQHPLADAPSLQLADLADSRWVVYSANMPMRLTLEREFHDADLRFPLHLMETTSAFT 242 Query: 248 TITLLAQS-NMVAVMPLDIAHFFAARQMLGILPVNLKTRIEHYGIVTRKNSTLSSLAKMF 306 T++LL ++ + VA++ +D+A F M ILP+ L TR E Y +VTR+ + LS +A++F Sbjct: 243 TLSLLQKNPHFVALLSVDVAQFCTRFGMTRILPLRLHTRSEPYQLVTRRGTMLSPVARLF 302 Query: 307 I 307 + Sbjct: 303 L 303 >gi|300310701|ref|YP_003774793.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 321 Score = 253 bits (645), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 134/303 (44%), Positives = 188/303 (62%), Gaps = 15/303 (4%) Query: 25 SVGSIVSRLRFRQVALLTALDEQGSLHKAAEVMHMTQPAATKALHEMEDALGVTLFDRSP 84 S+ S++SRLR +Q+ LL ALDE SLHKA+ + MTQ AA+K+L E+E L LF+R+ Sbjct: 16 SLNSLLSRLRMKQLQLLIALDEHKSLHKASSALAMTQSAASKSLAELESMLDAQLFERTK 75 Query: 85 RGIEATELGRCVIRYARL-------------KKKKNPGGRLSIGTIMGAAP-FVTRALSR 130 G+ A G CVIRYARL + + GGRL+IG +MGA P + A+ Sbjct: 76 SGLIANPFGHCVIRYARLMATDLGSLCEEMAQIRSGQGGRLAIGAVMGAVPALIVPAVDA 135 Query: 131 LREIQPDVSIEISEDTSARQLLLLDQGRIDLMIGRSSVSSQPNLYHYEMLRGEPMCIVSG 190 L+ P +SIEI EDTS L LLD+G +DL++ R+SVS+ P YHY+ L EP+ +V Sbjct: 136 LQRRHPALSIEIVEDTSLHMLDLLDEGHLDLLVARASVSAHPGKYHYQSLSEEPLSVVVS 195 Query: 191 IDHPLATAQKVRLQELADASWILYSSNMPMRIWIEHEFKLEGVKVPGNVIETASPFVTIT 250 +HP ++++L LA WI Y S+MP+ +E E L G+ +P N I TAS FVT+ Sbjct: 196 AEHPRPRGKELQLAALAGYRWITYPSHMPLHAVLEREMDLAGLSMPANTIATASTFVTVA 255 Query: 251 LLAQ-SNMVAVMPLDIAHFFAARQMLGILPVNLKTRIEHYGIVTRKNSTLSSLAKMFIQI 309 LL Q ++MV+++P +A F R ML ILPV L++ + GIVTRK LS + F+Q+ Sbjct: 256 LLQQGADMVSILPTAVAEMFVQRGMLRILPVKLRSMSQTIGIVTRKGGQLSGAGQAFVQL 315 Query: 310 LRQ 312 LR+ Sbjct: 316 LRE 318 >gi|300310375|ref|YP_003774467.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 313 Score = 172 bits (437), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 96/296 (32%), Positives = 165/296 (55%), Gaps = 14/296 (4%) Query: 31 SRLRFRQVALLTALDEQGSLHKAAEVMHMTQPAATKALHEMEDALGVTLFDRSPRGIEAT 90 +RL+ RQ+ LL ALD+ ++H+AA ++MTQPAA+K L ++ED LGV LF+R PRG+ T Sbjct: 12 ARLKTRQLLLLIALDDTRNIHRAASELNMTQPAASKQLKDLEDMLGVALFERLPRGMRPT 71 Query: 91 ELGRCVIRYARLKK-------------KKNPGGRLSIGTIMGAA-PFVTRALSRLREIQP 136 G +IR+AR+ K G++ +G I+ A + A++R++E P Sbjct: 72 IYGEAMIRHARMALTSLSKAHEDIVALKSGLSGQVDVGGILSPAMALLPPAIARVKEQAP 131 Query: 137 DVSIEISEDTSARQLLLLDQGRIDLMIGRSSVSSQPNLYHYEMLRGEPMCIVSGIDHPLA 196 + I + ++S L L G +D +IGR YE L EP+C V+ + HP Sbjct: 132 MLRIGVEVESSNILLSRLLHGELDFIIGRILDDEARGGLLYEELADEPICAVARVGHPFH 191 Query: 197 TAQKVRLQELADASWILYSSNMPMRIWIEHEFKLEGVKVPGNVIETASPFVTITLLAQSN 256 + L +LA +WI+ +R + F+ +G+ P NV++T + +LL Q++ Sbjct: 192 ARTDLSLADLASEAWIMAPKGSILRARCDQMFREQGLAPPSNVVDTTAILFITSLLQQTD 251 Query: 257 MVAVMPLDIAHFFAARQMLGILPVNLKTRIEHYGIVTRKNSTLSSLAKMFIQILRQ 312 + MP ++A ++ +++ ILP+ L R+ +GI+TR+++ LS A + +Q +R+ Sbjct: 252 FIYAMPTEVARYYTQARLMDILPIELPCRMAGFGIITRQDNLLSPGANLVLQAIRE 307 >gi|300313740|ref|YP_003777832.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 307 Score = 85.9 bits (211), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 75/294 (25%), Positives = 141/294 (47%), Gaps = 23/294 (7%) Query: 35 FRQVALLTALDEQGSLHKAAEVMHMTQPAATKALHEMEDALGVTLFDRSPRGIEATELGR 94 FR + T + E+G + +AAE ++ TQPA TK + +E+ LG LF+R RGI T +G Sbjct: 3 FRDLKYFTVIAEEGHVGRAAERLYKTQPALTKCIDRLEEQLGAPLFERLGRGIRLTAVGE 62 Query: 95 CVIRYARL------KKKKNPG-------GRLSIGTIMGAAPFVTRALSR--LREIQPDVS 139 ++ AR + + G G + +G + A V + + L E Q V+ Sbjct: 63 ALLNRARQISLMMDETAREIGDYAHGREGNIRLGCVPTLAEHVLPGVCQQLLGEAQ-KVT 121 Query: 140 IEISEDTSARQLLLLDQGRIDLMIGRSSVSSQPNLYHYEMLRGEPMCIVSGIDHPLATAQ 199 I++ + L L G +D+++G + L+H E + + M +++ HP+ + Sbjct: 122 IDLKVSMNDALLAGLKAGELDIVLG--PMIQDDELFHTEEIMQDQMVVMASPQHPIFD-K 178 Query: 200 KVRLQELADASWILYSSNMPMRIWIEHEFKLEGVKVPGNVIETASPFVTITLLAQSNMVA 259 K+RL+ L D W+L + ++ R W+++ F + P I + + L+ +SN++ Sbjct: 179 KIRLRHLLDYQWVLPAQSVLSRQWLDNVFDRHHLPRPKVQITPTVLNMILPLIERSNLLG 238 Query: 260 VM-PLDIAHFFAARQMLGILPVNLKTRIEHYGIVTRKNSTLSSLAKMFIQILRQ 312 L+++ R L + + T + G+ R++ LS A+ I+IL + Sbjct: 239 FASKLNLSE---GRGHLREVVLKETTMLRRMGLTYRRDMYLSPAAQRLIRILHE 289 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 913,955 Number of extensions: 35824 Number of successful extensions: 242 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 228 Number of HSP's successfully gapped: 21 Length of query: 314 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 229 Effective length of database: 1,217,395 Effective search space: 278783455 Effective search space used: 278783455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)