BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0147.p (397 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310161|ref|YP_003774253.1| aminohydrolase [Herbaspirillum ... 456 e-129 gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoac... 375 e-105 gi|300310472|ref|YP_003774564.1| hippurate hydrolase [Herbaspiri... 369 e-103 >gi|300310161|ref|YP_003774253.1| aminohydrolase [Herbaspirillum seropedicae SmR1] Length = 402 Score = 456 bits (1173), Expect = e-129, Method: Compositional matrix adjust. Identities = 221/385 (57%), Positives = 270/385 (70%), Gaps = 5/385 (1%) Query: 13 DLQKIRRDIHAHPELCYEEVRTADVVAQKLTEWGIPVVRGLGVTGVVGIIKKGESPRAIG 72 DL IRR +HAHPEL +EE RTA VA L +G+ V +G GVV ++KG+S RAI Sbjct: 16 DLVSIRRHLHAHPELRFEEKRTASFVADMLRSYGLTVAENIGGYGVVATLRKGKSTRAIA 75 Query: 73 LRADMDALPMSEINTFDHASRHPGKMHACGHDGHTAMLLGAARYLAQHGDFDGTVYVIFQ 132 LRADMDALPMSE N F H S GKMHACGHDGHT MLLGAAR L++ +FDGTV+ +FQ Sbjct: 76 LRADMDALPMSEQNDFSHISTCAGKMHACGHDGHTTMLLGAARRLSREVEFDGTVHFVFQ 135 Query: 133 PAEEGGRGAERMIQDGLFEKYPMDAVFGMHNWPGIPAGHFGVTPGPQMASSNEFHVTVKG 192 PAEEGG GA MI+DGLFE++P DA+FG+HNWPG+PAG FG+ PGP MASSN F T+ G Sbjct: 136 PAEEGGAGARLMIEDGLFERFPADAIFGVHNWPGLPAGSFGLRPGPLMASSNTFSATLFG 195 Query: 193 RGSHAAQPHKAADPVMTAVHIAQAWQSIVARNVNPNDPAVVSITQIHTGSATNVIPDEAM 252 RG+H AQPH++ DPV+ A + AWQSIV RN+NPN AV+S+TQ+HTG+A NVIP++A Sbjct: 196 RGAHGAQPHRSIDPVLAAAQLTLAWQSIVTRNINPNHRAVISVTQLHTGTADNVIPEQAT 255 Query: 253 MVGTVRTFSLPVLDLIERRMQEIAEHTAAAFDATVDFRFNRNYPPLINHAKETAFAVEVL 312 + GTVR+F LDLIE RM IAE FD V F F R YP ++NH + T A++ + Sbjct: 256 LSGTVRSFDAETLDLIEHRMAAIAEAIGQMFDLRVQFHFERLYPAVVNHPEATQQAMQAM 315 Query: 313 TEQFGADHVDAKTEPTMGAEDFAFMLQHKPGCYVFLGNG---DGGHRDQGHGLGPCNLHN 369 GA+ VD TEP +EDFAF LQHKPGCY FLGNG DG + G LH+ Sbjct: 316 MAAVGAEQVDGDTEPAFASEDFAFYLQHKPGCYAFLGNGVIADG--KQAGSQATSRELHS 373 Query: 370 PSYDFNDDLLPIGATYWVRLAEKFL 394 P YDFNDD++ G YWV L ++L Sbjct: 374 PFYDFNDDIIDAGVAYWVSLVRQYL 398 >gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoacylase/carboxypeptidase [Herbaspirillum seropedicae SmR1] Length = 399 Score = 375 bits (963), Expect = e-105, Method: Compositional matrix adjust. Identities = 186/384 (48%), Positives = 254/384 (66%), Gaps = 12/384 (3%) Query: 13 DLQKIRRDIHAHPELCYEEVRTADVVAQKLTEWGIPVVRGLGVTGVVGIIKKGESPRAIG 72 ++ IRR +H HPEL +EEV TA +VAQ+L +WG V R +G G+V ++ G S R+IG Sbjct: 21 EITAIRRHLHQHPELSFEEVDTAALVAQRLEQWGYAVTRHIGGNGLVATLRVGSSARSIG 80 Query: 73 LRADMDALPMSEINTFDHASRHPGKMHACGHDGHTAMLLGAARYLAQHGDFDGTVYVIFQ 132 LRADMDALP+ E D AS PG MHACGHDGHTAMLLGAAR+LA+ FDGT+ +IFQ Sbjct: 81 LRADMDALPIQEETGLDWASVKPGAMHACGHDGHTAMLLGAARHLARTRRFDGTLNLIFQ 140 Query: 133 PAEEGG--RGAERMIQDGLFEKYPMDAVFGMHNWPGIPAGHFGVTPGPQMASSNEFHVTV 190 PAEE G GA++M+ DGLFE++P +AVFG+HN PG+ AG F GP MA+ + + + Sbjct: 141 PAEEAGFDSGAQKMLADGLFERFPCEAVFGIHNHPGVEAGTFMFRSGPFMAACDTVKIRI 200 Query: 191 KGRGSHAAQPHKAADPVMTAVHIAQAWQSIVARNVNPNDPAVVSITQIHTGSATNVIPDE 250 GRGSHAA+PH + DPV+ A + A Q++V+RN++P D AVV++ +H G A+NVIP+ Sbjct: 201 TGRGSHAARPHLSVDPVVVAASLVMALQTVVSRNIDPMDSAVVTVGSLHAGKASNVIPEF 260 Query: 251 AMMVGTVRTFSLPVLDLIERRMQEIAEHTAAAFDATVDFRFNRNYPPLINHAKETAFAVE 310 A M +VR+F V +L+E+R++ + A ++ A + + R YP L+N ET FA Sbjct: 261 ATMELSVRSFKPEVRELLEQRIRALVSTHAQSYGAQAEIDYLRGYPVLVNSDAETDFARS 320 Query: 311 VLTEQFGADHVDAKTEPTMGAEDFAFMLQHKPGCYVFLGNGDGGHRDQGHGLGPCNLHNP 370 V E G + V A P G+EDFA+ L+ +PGC++ +GNG G LHN Sbjct: 321 VAEELVGPEKVIAPFGPIAGSEDFAYFLRQRPGCFLRVGNGQ----------GKPMLHNA 370 Query: 371 SYDFNDDLLPIGATYWVRLAEKFL 394 YDFND +PIGA YW RL E+++ Sbjct: 371 GYDFNDANIPIGAAYWTRLVERWM 394 >gi|300310472|ref|YP_003774564.1| hippurate hydrolase [Herbaspirillum seropedicae SmR1] Length = 403 Score = 369 bits (946), Expect = e-103, Method: Compositional matrix adjust. Identities = 185/390 (47%), Positives = 241/390 (61%), Gaps = 7/390 (1%) Query: 5 DPIVQFHADLQKIRRDIHAHPELCYEEVRTADVVAQKLTEWGIPVVRGLGVTGVVGIIKK 64 D + Q A +RRD+HAHPEL +EE RTADVV ++L G V RGLG TGVV + Sbjct: 9 DTLHQHRAHWAGLRRDLHAHPELRFEEHRTADVVVRELQSLGYTVTRGLGGTGVVASLAG 68 Query: 65 GESPRAIGLRADMDALPMSEINTFDHASRHPGKMHACGHDGHTAMLLGAARYLAQHGDFD 124 + R I LRAD+DALP+ E N F HAS G MHACGHDGHT MLLGAAR L Q Sbjct: 69 ADPQRGIVLRADLDALPIVEANDFAHASCTHGVMHACGHDGHTVMLLGAARLLKQGPMLP 128 Query: 125 GTVYVIFQPAEEGGRGAERMIQDGLFEKYPMDAVFGMHNWPGIPAGHFGVTPGPQMASSN 184 G+V+ +FQP EEGG GA +MI DGLFE++P +AVFGMHNWPG+PAG FG+ PGP MA+ + Sbjct: 129 GSVHFVFQPGEEGGAGARKMIDDGLFEQFPTEAVFGMHNWPGLPAGQFGLRPGPIMAAGS 188 Query: 185 EFHVTVKGRGSHAAQPHKAADPVMTAVHIAQAWQSIVARNVNPNDPAVVSITQIHTGSAT 244 +T+ G+G+HAAQPH DP+ A + Q+I AR+ +P DPAV+S+ H G Sbjct: 189 RLKITITGKGAHAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVCMFHAGDTD 248 Query: 245 NVIPDEAMMVGTVRTFSLPVLDLIERRMQEIAEHTAAAFDATVDFRFNRNYPPLINHAKE 304 NVIPD A + GT+RT S + ++ ++ + A A+ A V+ F + YP IN E Sbjct: 249 NVIPDRAELRGTIRTLSSTLQQKLQEDIRRMCHALAEAYGAKVEVEFFQYYPATINTPAE 308 Query: 305 TAFAVEVLTEQFGADHVDAKTEPTMGAEDFAFMLQHKPGCYVFLGNGDGGHRDQGHGLGP 364 T F V+ + FG D + M +EDF FML+ +PG YV +GN Sbjct: 309 TDFCERVIRQTFGDDRIRTGIPANMTSEDFGFMLEERPGAYVLIGNAPQDRASH------ 362 Query: 365 CNLHNPSYDFNDDLLPIGATYWVRLAEKFL 394 +LH+P YDFNDD++ G YWV LA+ + Sbjct: 363 -SLHHPHYDFNDDIIEAGVRYWVALAQGYF 391 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,340,486 Number of extensions: 58746 Number of successful extensions: 126 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 122 Number of HSP's successfully gapped: 3 Length of query: 397 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 310 Effective length of database: 1,207,925 Effective search space: 374456750 Effective search space used: 374456750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)