BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0146.p (208 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313160|ref|YP_003777252.1| threonine efflux protein [Herba... 217 6e-58 >gi|300313160|ref|YP_003777252.1| threonine efflux protein [Herbaspirillum seropedicae SmR1] Length = 207 Score = 217 bits (552), Expect = 6e-58, Method: Compositional matrix adjust. Identities = 134/189 (70%), Positives = 148/189 (78%), Gaps = 1/189 (0%) Query: 2 MLSSEFFLLAGAHLLALASPGPDFLLLLRGSLRHGRGYGLAAALGIALANGLYIALAIGG 61 M SSEF LLA AHLLALASPGPDFLLL+RG+LRHGRGYGLAA+ GIA ANGLYIALAI G Sbjct: 1 MFSSEFLLLASAHLLALASPGPDFLLLVRGALRHGRGYGLAASAGIAAANGLYIALAIAG 60 Query: 62 FSLLQRAPLLLAAMQWLAACYLAWLGWVFWRAGRHPAVLPAAPVSAARVAPWAALGTGFL 121 FSLLQ +P LL AMQWLA+ YLAWLGW+F RA PA P + A A W +G GFL Sbjct: 61 FSLLQHSPALLVAMQWLASLYLAWLGWMFMRASLEPAPWPGSAGRQA-AAAWRGVGAGFL 119 Query: 122 SAALNPKNGVFYFGLFTVIVSAQTGASTKLFYGVWMFGAVLAWDAGLVYFIAQQRVMRAL 181 SA LNPKNG+FYFGLFT++V A TG KLFYGVWMFGAVLAWDAGLV +AQ RV L Sbjct: 120 SALLNPKNGLFYFGLFTLMVEAHTGLPRKLFYGVWMFGAVLAWDAGLVCLMAQPRVAALL 179 Query: 182 TRVLPLIER 190 R LP++ER Sbjct: 180 DRALPMVER 188 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.140 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 429,031 Number of extensions: 12524 Number of successful extensions: 90 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 89 Number of HSP's successfully gapped: 3 Length of query: 208 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 127 Effective length of database: 1,236,335 Effective search space: 157014545 Effective search space used: 157014545 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 128 (53.9 bits)