BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0111.p (131 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Her... 107 3e-25 gi|300312286|ref|YP_003776378.1| signal-transducing histidine ki... 73 8e-15 gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [He... 72 2e-14 gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [He... 64 6e-12 gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbas... 57 5e-10 >gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 107 bits (268), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 54/71 (76%), Positives = 61/71 (85%) Query: 3 GVEQWSETRLAELIFEPGFSTQSQVSALAGRGIGMDVVRAEVAALGGRMTVHSRPGQGVL 62 G+ + S+ RLA+LIF+PG ST QVSALAGRGIGMDVVR+EVAALGG M VHS+PGQGV Sbjct: 528 GLAEASQARLADLIFQPGLSTSPQVSALAGRGIGMDVVRSEVAALGGWMRVHSQPGQGVQ 587 Query: 63 FTLYLPLSLAT 73 FTLYLPLSLA Sbjct: 588 FTLYLPLSLAV 598 >gi|300312286|ref|YP_003776378.1| signal-transducing histidine kinase CheA protein [Herbaspirillum seropedicae SmR1] Length = 697 Score = 73.2 bits (178), Expect = 8e-15, Method: Composition-based stats. Identities = 34/82 (41%), Positives = 53/82 (64%) Query: 1 LPGVEQWSETRLAELIFEPGFSTQSQVSALAGRGIGMDVVRAEVAALGGRMTVHSRPGQG 60 +P + S+ + +LIFEPGFST QV+ ++GRG+GMDVV+ + A+GG + + S G G Sbjct: 482 MPVTDNLSDAEVWQLIFEPGFSTAEQVTDVSGRGVGMDVVKRNILAMGGAVDIRSSKGFG 541 Query: 61 VLFTLYLPLSLATVXGKKKKKG 82 ++ LPL+LA + G + G Sbjct: 542 TTISISLPLTLAILDGMSIRIG 563 >gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 725 Score = 71.6 bits (174), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 32/71 (45%), Positives = 48/71 (67%) Query: 6 QWSETRLAELIFEPGFSTQSQVSALAGRGIGMDVVRAEVAALGGRMTVHSRPGQGVLFTL 65 + S+ + +FEPGFST QV+ L+GRG+GMDVVR + AL G +++ + G+G + L Sbjct: 512 ELSDAEIYRFVFEPGFSTARQVTELSGRGVGMDVVRRNIEALHGEVSIDTGRGRGTVVRL 571 Query: 66 YLPLSLATVXG 76 LPL+LA + G Sbjct: 572 RLPLTLAIISG 582 >gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 837 Score = 63.5 bits (153), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 31/70 (44%), Positives = 43/70 (61%) Query: 4 VEQWSETRLAELIFEPGFSTQSQVSALAGRGIGMDVVRAEVAALGGRMTVHSRPGQGVLF 63 +E+ S+ L+F GFST VS L+GRG+GMDVVR V + G + +HS G+G Sbjct: 637 LERISDQDAVNLVFSSGFSTAEVVSDLSGRGVGMDVVRNAVEKVNGSLALHSVSGKGTRL 696 Query: 64 TLYLPLSLAT 73 + LPLS+A Sbjct: 697 QISLPLSMAV 706 >gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbaspirillum seropedicae SmR1] Length = 638 Score = 57.0 bits (136), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 25/61 (40%), Positives = 41/61 (67%) Query: 14 ELIFEPGFSTQSQVSALAGRGIGMDVVRAEVAALGGRMTVHSRPGQGVLFTLYLPLSLAT 73 +L+F PGFST+ S+++GRG+GMDVV+ V G + + S G+G F++ LP+ L+ Sbjct: 446 QLVFLPGFSTKEVASSVSGRGVGMDVVKTAVEKHRGTIRIESTIGKGTKFSIRLPIELSI 505 Query: 74 V 74 + Sbjct: 506 I 506 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.132 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 303,495 Number of extensions: 9092 Number of successful extensions: 85 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 86 Number of HSP's successfully gapped: 6 Length of query: 131 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 56 Effective length of database: 1,264,745 Effective search space: 70825720 Effective search space used: 70825720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 125 (52.8 bits)