BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0003.0105.p
         (300 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313129|ref|YP_003777221.1| drug/metabolite transporter (DM...   522   e-149
gi|300311553|ref|YP_003775645.1| drug/metabolite transporter (DM...    65   8e-12

>gi|300313129|ref|YP_003777221.1| drug/metabolite transporter (DMT)
           superfamily permease [Herbaspirillum seropedicae SmR1]
          Length = 305

 Score =  522 bits (1344), Expect = e-149,   Method: Compositional matrix adjust.
 Identities = 255/300 (85%), Positives = 277/300 (92%)

Query: 1   MLVASFLFAVMGVCVKLASQYYTTSEIVLCRGVIGVLFILCLIRLRGGTLRTPFPRDHFI 60
           MLVASFLF++MGVCVKLAS YY+TSEIVLCRG+IG+  I  L+R+RGGT RTPFPRDH I
Sbjct: 6   MLVASFLFSIMGVCVKLASDYYSTSEIVLCRGLIGISVIAALVRVRGGTFRTPFPRDHLI 65

Query: 61  RGGVGVVSLWMWFYSFSLLPIATATTLNYMSSIWIAVMLFVAGWWQGHKKFEWGLAGTVA 120
           RGGVGV+SLW+WFYSFSLLP+ATATTLNYMSSIWIAVMLF A WWQG+KKFEWGLA TV 
Sbjct: 66  RGGVGVISLWLWFYSFSLLPVATATTLNYMSSIWIAVMLFAAAWWQGNKKFEWGLAATVV 125

Query: 121 LSFLGVALLMRPSLNAEQLLGGAISLASGVLSALVYLQVRKLGLLGEPEYRVVFYFSATG 180
           LSF+GVALLMRPSL+A+QLLGGAISL SGVLSALVYLQVRKLGLLGEPEYRVVFYFS TG
Sbjct: 126 LSFIGVALLMRPSLDADQLLGGAISLFSGVLSALVYLQVRKLGLLGEPEYRVVFYFSLTG 185

Query: 181 VLAGLVGSVVTGRVPLWHSHSWIGALLVLMIGLTATTAQIAMTRAYRLGNTLVTANLQYT 240
           VLAGLVGSV TGRVPLWHSHSW GA LV+MIGLTAT AQIAMTRAYRLGNTLVTANLQYT
Sbjct: 186 VLAGLVGSVATGRVPLWHSHSWQGAALVIMIGLTATLAQIAMTRAYRLGNTLVTANLQYT 245

Query: 241 GIVFSSLFGVLIWDDAPGWIGWLGMAIILASGMLATYRNQRKSQAVAKNVPKVDPIATEV 300
           GIVFSSLFGVL+W+DAPGW+GWLGM +ILASG+LATY N R SQ  A+N+PKVDPIATEV
Sbjct: 246 GIVFSSLFGVLVWNDAPGWLGWLGMGVILASGLLATYHNHRSSQIAARNLPKVDPIATEV 305


>gi|300311553|ref|YP_003775645.1| drug/metabolite transporter (DMT)
           superfamily permease [Herbaspirillum seropedicae SmR1]
          Length = 314

 Score = 64.7 bits (156), Expect = 8e-12,   Method: Compositional matrix adjust.
 Identities = 67/244 (27%), Positives = 114/244 (46%), Gaps = 25/244 (10%)

Query: 61  RGGVGVVSLWMWFYSFSLLPIATATTLNYMSSIWIAVMLFVAGWWQGHKKF--EWGLAGT 118
           RG + + S  ++F    ++P+A  T +N+ + +   ++L ++ W  G + +   W     
Sbjct: 89  RGALLLGSTLIFFSVLKIVPLAEGTAMNFCAPL---IVLAISPWLLGERSYLSRWI---A 142

Query: 119 VALSFLGVALLMRPSLNAEQLLGGAISLASGVLSALVYLQVRKLGLLGEPEYRVVFYFSA 178
           V + F G+ +++RPS +     G A+ L S +  AL+ +  RK      P   V  ++ A
Sbjct: 143 VLVGFTGMLIVIRPSGDIPPY-GVALGLISALTQALLSILNRKASQADNP--MVTLFYGA 199

Query: 179 TGVLAGLVGSVV-TGRVPL-WHSHSWIGALLVLMI--GLTATTAQIAMTRAYRLGNTLVT 234
                 LVGSVV T  VP  W SH+     L +++  G+T+T        AYR     V 
Sbjct: 200 ------LVGSVVSTLLVPFFWTSHTPTMVELAILVSTGITSTIGHFLQNSAYRHAEASVL 253

Query: 235 ANLQYTGIVFSSLFGVLIWDDAPGWIGWLGMAIILASGMLATYRNQRKSQAVAKNVPKVD 294
               Y  I+ +   G L++   P  +  LG+AII ASG+   Y   ++    A  +P  D
Sbjct: 254 TPFFYAQIISACGMGWLVFGQFPDRVTVLGIAIICASGIGIAYIEHKR----AHPLPPAD 309

Query: 295 PIAT 298
           P+ +
Sbjct: 310 PMES 313


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.327    0.140    0.442 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 964,070
Number of extensions: 42060
Number of successful extensions: 198
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 197
Number of HSP's successfully gapped: 2
Length of query: 300
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 215
Effective length of database: 1,217,395
Effective search space: 261739925
Effective search space used: 261739925
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 130 (54.7 bits)