BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0090.p (693 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311919|ref|YP_003776011.1| non-hemolytic phospholipase C (... 583 e-167 gi|300310916|ref|YP_003775008.1| phospholipase C protein [Herbas... 89 2e-18 >gi|300311919|ref|YP_003776011.1| non-hemolytic phospholipase C (phosphatidylcholine choline phospho hydrolase) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 696 Score = 583 bits (1502), Expect = e-167, Method: Compositional matrix adjust. Identities = 320/680 (47%), Positives = 419/680 (61%), Gaps = 34/680 (5%) Query: 18 SISKALAIAPQVRTGSIEDVEHIVVFMQENRSFDHYLGHLSGVRGYNDRFPVTLPNGRPV 77 SI KALA+AP TG+I+DV+H+V+ MQENRSFDHY G L GVRG+ D P LP+G PV Sbjct: 31 SIRKALAVAPASPTGTIKDVKHVVILMQENRSFDHYFGCLRGVRGFADPIPRPLPDGSPV 90 Query: 78 WFQPRQEDPAAVIAPFRYDTTDPAMNAQCVGSLPHTWATTQGAINGGRADQWAQ-QKGNL 136 + Q ++ ++ P+R + + + G+LPH W A N G D+W + +K + Sbjct: 91 FHQ--RDGQGRLVLPYRMEMATTSA-GRAGGNLPHAWKDQHAAWNRGDYDKWIEAKKDTV 147 Query: 137 CMGYHVREDIPFHYALADAFTVCDQYFCSIPGNTHPNRMYLMTGMIDPLGSGGGPLLDNT 196 M Y RED+PFHYALADAFT+CD Y CS+ G+T+PNR +LM+G ID G GGP+ Sbjct: 148 TMAYFTREDLPFHYALADAFTLCDAYHCSVLGSTNPNRFHLMSGGIDLQGRAGGPMT--- 204 Query: 197 DYIDNQFSSTKLP--------PFTWTTYPERLEQAGISWQVYQQGTDFDKFSGNYGTN-- 246 + S + +P F WTTYPERLEQ G+SW+VYQ + K G+Y ++ Sbjct: 205 -FQPRTGSCSGVPGERRSGADSFGWTTYPERLEQHGVSWKVYQGTEELHKDHGDYPSDFN 263 Query: 247 -MLASFENFVHAPIGSSLHARGMSTRSVAQLRADVEQGKLPQVSWLLPPAVYSEHPDYTP 305 + F + +A S L + S ++A L DV GKLPQVSWL+PP + EHPD TP Sbjct: 264 VLQYFFAQYQNAAPDSPLWQKACSKFTLADLARDVNSGKLPQVSWLMPPKLSCEHPDRTP 323 Query: 306 LYGARYLSDILDALTANPEVWSKTVLFIMYDENDGFFDHVVPPQAPTVPGSGKSTVDIAL 365 YGA Y+S +LD LTANP +WS TVL + YDENDGFFDHVVPP P G G STV+ + Sbjct: 324 GYGAHYISQVLDILTANPALWSSTVLLVNYDENDGFFDHVVPPTPPLHAGQGLSTVETSA 383 Query: 366 ERHAVVAASQQGKFT--ADQLPYGLGPRVPMFVVSPWSRGGRVNSQVFDHTSVLQFIERR 423 E H ++G + AD LP GLG RVPM V+SPWS+GG V S+VFDHTSV++F+E+ Sbjct: 384 EFH------RKGDYVNPADDLPAGLGARVPMMVISPWSKGGYVCSEVFDHTSVIRFLEQV 437 Query: 424 FGVIEPNISPWRRAVCGDLSSTLDFSRRDTSAPRLPDTTHYLADSDLQCKRPVAISAPAP 483 F V EPNISPWRRAVCGDL+S DFSRRD ++P LPDT+ Y A SD Q + P ++ +P Sbjct: 438 FDVHEPNISPWRRAVCGDLTSAFDFSRRDAASPVLPDTSAYRALSDAQARLPAPVAPSSP 497 Query: 484 GAALSIAPQEAGVRTACALPYALHVHGSREGDRYILRFD--NVGRQGAHFQVHDDVLGSA 541 ++ QE G+R A ALPY L V D+ L+ D N G+ G +QV+ Sbjct: 498 ----AMPQQERGMRPARALPYVLAVDDRVVPDQDALQLDFLNPGKAGVVYQVYARQESER 553 Query: 542 PRGYTVEAGKQLGDSWP-LAAGGRYQFSVWGPNGYFRRFAGQAGSTSQPEPELRSHYDAA 600 + +TV+ GK L W + G Y V+GPNG+ R G EP +++ +D Sbjct: 554 YQTFTVQPGKSLTAQWRGVGPVGGYDLKVYGPNGFLRELRGSLERGGGVEPVVQARHDGE 613 Query: 601 RGVLNLSLLNPGPAPLIATVTDLAYGHTPRVLHVPAGQSVSSAWDLAASHHWYDLQWQLQ 660 RG L L+LLNPG L + D A+G R + + GQS + W LA+SHHWYDL Q + Sbjct: 614 RGELVLALLNPGRQALHLVLRDQAHGQATRTVTLAPGQSATERWPLASSHHWYDLLVQAE 673 Query: 661 DRPHWLRRLAGHLETGRPSM 680 DRP + RRLAGH+ETGRPS Sbjct: 674 DRPAFARRLAGHVETGRPSF 693 >gi|300310916|ref|YP_003775008.1| phospholipase C protein [Herbaspirillum seropedicae SmR1] Length = 558 Score = 88.6 bits (218), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 122/505 (24%), Positives = 185/505 (36%), Gaps = 152/505 (30%) Query: 35 EDVEHIVVFMQENRSFDHYLGHLSGVRG-----------YNDRFPVTLPNGRPVWF---- 79 E V+ IVV ENRSF++ G+ DR L P W Sbjct: 61 EQVKTIVVIYAENRSFNNLFADFPGLEKPLSALKPSDYQQRDRDGSLLKQLPPAWSGVVN 120 Query: 80 QPRQEDPAAVIAPFRYDTTDP----AMNAQCVGSLP---------HTWATTQGAINGGRA 126 Q + + A +Y T P A+ +LP H + Q INGG+ Sbjct: 121 QEQTVEGVTYEAGKQYQTNLPNAPYALKGPQGEALPLGLVTRDLWHVFYQNQMQINGGKN 180 Query: 127 D---QWAQQKGNLCMGYHVREDIPFHYALADA---FTVCDQYFCSIPGNT---------- 170 D WA G L MG++ D +H L D F +CD +F G + Sbjct: 181 DGFVAWAD-SGGLTMGHYA--DTRYHLRLWDVAREFVLCDNFFQGAFGGSFLNHQYLICA 237 Query: 171 ----HPNRMYLM-------TGMIDPL--------GS-----GGGPLLDNTDYIDNQFSST 206 +PN M + T DPL GS G P + + ++ Sbjct: 238 TAPYYPNVMNSVSRTQVAKTKNNDPLSPELVPAEGSPTSALQGPPKFGPSALTPDGYAVN 297 Query: 207 KLPPFTWTTYP--------------------------ERLEQAGISWQVYQ---QGTDFD 237 + P W T+ ++L + GI W Y Q T D Sbjct: 298 TMAPPYWPTWSRDRNNPAYAEPNQPSVLVPQTHEHIGDKLNKKGIDWAWYAGAWQAT-LD 356 Query: 238 KFSGNYGTNMLASFENFVHAPIG--------------SSLHARGMSTRS-VAQLRADVEQ 282 +F + G + +F+ + H P L G+ + + ADVE Sbjct: 357 EFKDSKGIPKIPNFQ-YHHQPFNYFKNLGPENPAERNKRLRDGGLGDEARTNRFLADVEA 415 Query: 283 GKLPQVSWLLPPAVYSEHPDYTPL-YGARYLSDILDALTANPEVWSKTVLFIMYDENDGF 341 G+LP V++ P + H Y + G R+++ I+ +L A+P+ W V+ + +DEN G+ Sbjct: 416 GRLPPVTFYKPQGNLNLHAGYADIASGDRHIAHIIKSLQASPQ-WKNMVVVVTFDENGGW 474 Query: 342 FDHVVPPQAPTVPGSGKSTVDIALERHAVVAASQQGKFTADQLPYGLGPRVPMFVVSPWS 401 +DHV PP+ +G G RVP VVSP++ Sbjct: 475 WDHVAPPKGDR---------------------------------WGPGTRVPALVVSPFA 501 Query: 402 RGGRVNSQVFDHTSVLQFIERRFGV 426 R G V+ V+D S+L+ I R F + Sbjct: 502 RKGTVDHTVYDTASILRLITRTFDL 526 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,427,556 Number of extensions: 113279 Number of successful extensions: 298 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 287 Number of HSP's successfully gapped: 2 Length of query: 693 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 601 Effective length of database: 1,184,250 Effective search space: 711734250 Effective search space used: 711734250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)