BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0081.p (256 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 499 e-143 gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herb... 157 5e-40 gi|300310680|ref|YP_003774772.1| 3-oxoacyl-[acyl-carrier-protein... 149 2e-37 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 145 3e-36 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 139 2e-34 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 499 bits (1286), Expect = e-143, Method: Compositional matrix adjust. Identities = 246/256 (96%), Positives = 250/256 (97%) Query: 1 MNRNDSGARVALVTGGAMGIGASISAQLAAKGHTVLVADINLEAASKTAHALVAAGHRAA 60 MNRNDSGARVALVTGGAMGIGASIS QLAAKGHTVLVADINL+AASKTA ALVAAGHRAA Sbjct: 1 MNRNDSGARVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAA 60 Query: 61 ALQMDLGSPEAIAQAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLDNWLQTMNVNVTGV 120 LQMDLGSPE+IA AFAHIEKDYGRCDVLVNNAGVARTYSFLDYPL+NWLQTMNVNVTGV Sbjct: 61 PLQMDLGSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGV 120 Query: 121 LLAGQHAARLMVSKGWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGI 180 LLAGQHAARLMV + WGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGI Sbjct: 121 LLAGQHAARLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGI 180 Query: 181 TVNSVAPGPVDTPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGH 240 TVNSVAPGPVDTPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGH Sbjct: 181 TVNSVAPGPVDTPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGH 240 Query: 241 VIPVDGGFVAGGVLEI 256 VIPVDGGFVAGGVLEI Sbjct: 241 VIPVDGGFVAGGVLEI 256 >gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 157 bits (398), Expect = 5e-40, Method: Compositional matrix adjust. Identities = 107/248 (43%), Positives = 143/248 (57%), Gaps = 12/248 (4%) Query: 8 ARVALVTGGAMGIGASISAQLAAKGHTVLVADINLEAASKTAHALV----AAGHRAAALQ 63 ++VA V GG+ GIGA+I +LAA+G TV ++ + A ALV A G RA A+Q Sbjct: 17 SKVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVS---SPDKAQALVSSVQAKGGRALAIQ 73 Query: 64 MDLGSPEAIAQAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLDNWLQTMNVNVTGVLLA 123 D A+ QA + +GR D+LVNNAGV + L++ +T+ VNV V +A Sbjct: 74 ADSADAAALQQAIRLAAETFGRLDILVNNAGVLAMGPLDQFKLEDLDRTLAVNVRSVFVA 133 Query: 124 GQHAARLMVSKGWGRIVNISSISGIR-AGAGRTAYGTSKAAVIGLTRQMAIELAQYGITV 182 Q AAR M GR+++I S + R AG Y SK+A++GLT+ +A +L GITV Sbjct: 134 TQEAARYMGE--GGRVISIGSTNADRIPFAGGAVYAMSKSALVGLTKGLARDLGPRGITV 191 Query: 183 NSVAPGPVDTPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGHVI 242 N+V PGPVDT M A S S L+ + RYG +EIAS V YLA ES YITG + Sbjct: 192 NNVQPGPVDTDMNPA--SGDFAASLTGLMALPRYGKAEEIASFVAYLAGPESGYITGANL 249 Query: 243 PVDGGFVA 250 +DGGF A Sbjct: 250 MIDGGFSA 257 >gi|300310680|ref|YP_003774772.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 247 Score = 149 bits (376), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 87/241 (36%), Positives = 136/241 (56%), Gaps = 1/241 (0%) Query: 9 RVALVTGGAMGIGASISAQLAAKGHTVLVADINLEAASKTAHALVAAGHRAAALQMDLGS 68 ++ALVTG A G+G SI+ ++ A G+ V +ADI+LE S A +L A+G A L +D+ Sbjct: 4 KIALVTGAARGLGESIARKMHAAGYKVALADIDLEQVSAVAASLDASGATAMPLALDVRR 63 Query: 69 PEAIAQAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLDNWLQTMNVNVTGVLLAGQHAA 128 A IE +G VLVNNAG +R + ++ + W + +N+ G LA Q Sbjct: 64 KPDFIAACDAIELRWGGAAVLVNNAGRSRVAALMEITPEEWDEVTAINMKGTFLACQVFG 123 Query: 129 RLMVSKGWGRIVNISSISGIRAGAGRTA-YGTSKAAVIGLTRQMAIELAQYGITVNSVAP 187 + +G+GRI+NI+S++G G A Y +K AV+ LT+ A ELA G+TVN+++P Sbjct: 124 KRFADRGYGRIINIASLAGQNGGTATGAHYAAAKGAVLTLTKVFARELAGAGVTVNAISP 183 Query: 188 GPVDTPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGHVIPVDGG 247 GP+D P+ + + + +P++ G D IA AV LA+ E+ TG + ++GG Sbjct: 184 GPLDLPIVREIVPADKLAATIATIPVKALGEPDFIADAVLLLASPEAGSATGACLDINGG 243 Query: 248 F 248 Sbjct: 244 L 244 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 145 bits (367), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 84/244 (34%), Positives = 130/244 (53%), Gaps = 2/244 (0%) Query: 9 RVALVTGGAMGIGASISAQLAAKGHTVLV-ADINLEAASKTAHALVAAGHRAAALQMDLG 67 R+A VTGG GIG +I +L G+TV+ N K + G A + ++ Sbjct: 4 RIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQGFDIHASEGNVS 63 Query: 68 SPEAIAQAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLDNWLQTMNVNVTGVLLAGQHA 127 + E+ AF + + G DVLVNNAG+ R F D+W ++ N+ + + Sbjct: 64 NWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNSLFNVTKQV 123 Query: 128 ARLMVSKGWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSVAP 187 M +G+GRI+NISS++G + G+T Y T+KA + G T +A E+A G+TVN+V+P Sbjct: 124 IEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTVSP 183 Query: 188 GPVDTPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGHVIPVDGG 247 G + T M +A+ E E +P++R G +EIAS V ++A+ E Y TG ++GG Sbjct: 184 GYIGTDMVRAIRPE-VLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFSLNGG 242 Query: 248 FVAG 251 G Sbjct: 243 LYMG 246 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 139 bits (351), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 86/244 (35%), Positives = 134/244 (54%), Gaps = 2/244 (0%) Query: 9 RVALVTGGAMGIGASISAQLAAKGHTVLVADINLEAASKTAHALVAAGHRAA-ALQMDLG 67 +VALVTG + GIG +I+ +LA +G V+ + A+ L A G A +++ Sbjct: 9 QVALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPEAGRGAVLNVN 68 Query: 68 SPEAIAQAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLDNWLQTMNVNVTGVLLAGQHA 127 EA ++KD+G +LVNNAG+ + + + W + N+T V + Sbjct: 69 DAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRLSRAV 128 Query: 128 ARLMVSKGWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSVAP 187 R M+ GRI+NI+S+ G G+ Y +KA V G++R +A E+ ITVN VAP Sbjct: 129 LRGMMKARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNITVNCVAP 188 Query: 188 GPVDTPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGHVIPVDGG 247 G +DT MT+A++ EQ + + +P+ R G +EIA AV +LA+ ++AYITG + V+GG Sbjct: 189 GFIDTDMTRALN-EQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGTTLNVNGG 247 Query: 248 FVAG 251 G Sbjct: 248 MYMG 251 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.131 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 706,580 Number of extensions: 27105 Number of successful extensions: 248 Number of sequences better than 1.0e-08: 52 Number of HSP's gapped: 169 Number of HSP's successfully gapped: 52 Length of query: 256 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 173 Effective length of database: 1,226,865 Effective search space: 212247645 Effective search space used: 212247645 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)