BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0080.p (266 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogena... 521 e-149 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 137 1e-33 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 130 1e-31 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 130 1e-31 gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 127 1e-30 >gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 266 Score = 521 bits (1342), Expect = e-149, Method: Compositional matrix adjust. Identities = 252/266 (94%), Positives = 261/266 (98%) Query: 1 MDKLMQDKAIIVTGAGSVGEGWGNGKAAAVLYAREGGRVLAVDRSIEAALETQEIIRREG 60 MDKLMQ KAIIVTGAGSVGEGWGNGKAAAVLYAREGGRVLAVDRSIEAAL+TQ+IIR EG Sbjct: 1 MDKLMQGKAIIVTGAGSVGEGWGNGKAAAVLYAREGGRVLAVDRSIEAALQTQDIIRSEG 60 Query: 61 GVCEVLAADVSRSADVKAIAEAAMSHFGRVDVLHNNVGIAEVGGPVETSEESWNRLVAVN 120 GVCEVLAADVSR+ +VKAIAEAAM HFGRVDVLHNNVGIA+VGGPVETSEESWNRLVAVN Sbjct: 61 GVCEVLAADVSRNTEVKAIAEAAMDHFGRVDVLHNNVGIADVGGPVETSEESWNRLVAVN 120 Query: 121 QTSLFLTHKYVLPIMEKQRKGAIVNISSMAALRWIGFPYLGYTATKAAILAFTKNVAMQY 180 QTSLFLTHK+VLPIMEKQRKGAIVNISS+AALRWIGFPYLGYTATKAAILAFTKNVAMQY Sbjct: 121 QTSLFLTHKHVLPIMEKQRKGAIVNISSLAALRWIGFPYLGYTATKAAILAFTKNVAMQY 180 Query: 181 APMGIRANCVLPGMMDTPMIREPLKASYGGDIEEMRAKRNAQCPMGFMGDAWDVAHASLF 240 APMGIRANCVLPGMMDTPMIREPLKASYGGDIE+MRAKRNAQCPMGFMGDAWDVAHASLF Sbjct: 181 APMGIRANCVLPGMMDTPMIREPLKASYGGDIEQMRAKRNAQCPMGFMGDAWDVAHASLF 240 Query: 241 LASDNARYITGLDMIVDGGLSLRCVG 266 LASD+ARYITGLDMIVDGGLSLRC G Sbjct: 241 LASDHARYITGLDMIVDGGLSLRCAG 266 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 137 bits (344), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 95/265 (35%), Positives = 138/265 (52%), Gaps = 19/265 (7%) Query: 5 MQDKAIIVTGAGSVGEGWGNGKAAAVLYAREGGRVLAVDRSIEAALETQEIIRREGGVCE 64 +QDK IVTG GS + A YAREG ++ D L E I+ GG Sbjct: 3 LQDKVAIVTGGGSGFG-----EGIAKAYAREGAAIVVADIGEAGGLRVVEEIKAAGGRAV 57 Query: 65 VLAADVSRSADVKAIAEAAMSHFGRVDVLHNNVGIAEVGGP-VETSEESWNRLVAVNQTS 123 ADVS+ AD+ + A+ HFG++D++ NN G P +E E+ ++R+ AVN S Sbjct: 58 FAKADVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVKS 117 Query: 124 LFLTHKYVLPIMEKQRKGAIVNISSMAALRWIGFPYLG---YTATKAAILAFTKNVAMQY 180 +FL+ K +P + GA +NI+S A +R P G Y +K A++ +K++A + Sbjct: 118 IFLSAKTFVPYFRQVGGGAFINIASTAGIR----PRPGLTWYNGSKGAVITTSKSMAAEL 173 Query: 181 APMGIRANCVLPGMMDTPMIREPLKASYGGDIEEMRAKRNAQCPMGFMGDAWDVAHASLF 240 P IR NCV P + T ++ E + D E R K A PMG D+A+A L+ Sbjct: 174 GPDKIRVNCVNPVISATGLLSEFMGVP---DTPENRKKFVATIPMGRFSTPEDIANACLY 230 Query: 241 LASDNARYITGLDMIVDGGLSLRCV 265 L SD A ++TG+ + VDGG RCV Sbjct: 231 LGSDEAEFVTGVCIEVDGG---RCV 252 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 130 bits (327), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 84/260 (32%), Positives = 137/260 (52%), Gaps = 18/260 (6%) Query: 4 LMQDKAIIVTGAGSVGEGWGNGKAAAVLYAREGGRVLAVDRSIEAALETQEIIRREGGVC 63 L++DK ++TG + G G A A L A +G RV+ +D + R G Sbjct: 2 LLKDKVAVITGGAGIN---GLGYATARLMAEQGARVVVLDLAQADPAAAAA---RIGSQH 55 Query: 64 EVLAADVSRSADVKAIAEAAMSHFGRVDVLHNNVGIAEVGGPVETSEESWNRLVAVNQTS 123 L ADV+ A + A A + FGR+D+L NN GI + +E + ++R++ V+ Sbjct: 56 LGLVADVTDKASCETAAAAILQQFGRIDILVNNAGITQPVKTLEITGADYDRILDVSLRG 115 Query: 124 LFLTHKYVLPIMEKQRKGAIVNISSMAALR---WIGFPYLGYTATKAAILAFTKNVAMQY 180 + VLP M Q+ G+I+ ISS++A R +G P+ Y+A KA +L + +A ++ Sbjct: 116 TLYMSQAVLPAMRAQQNGSIICISSVSAQRGGGILGGPH--YSAAKAGVLGLARAMAREF 173 Query: 181 APMGIRANCVLPGMMDTPMIREPLKASYGGDIEEMRAKRNAQCPMGFMGDAWDVAHASLF 240 P GIR N + PG++ T +I+ L EE +A+ P+ +G A D+ A +F Sbjct: 174 GPEGIRVNSITPGLIGTDIIKGKLT-------EEKKAEIAETIPLARLGRADDIGGACVF 226 Query: 241 LASDNARYITGLDMIVDGGL 260 L SD ++Y TG+ + V+GG+ Sbjct: 227 LGSDLSQYCTGITLDVNGGM 246 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 130 bits (326), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 91/261 (34%), Positives = 146/261 (55%), Gaps = 20/261 (7%) Query: 4 LMQDK-AIIVTGAGSVGEGWGNGKAAAVLYAREGGRVLAVDRSIEAALETQEIIRREGGV 62 L++DK AII GAG G G+ + A L A +G R++ +D +E A E R G Sbjct: 2 LLKDKVAIITGGAGQNGLGF----STARLMAAQGARIVILD--LERA-EPAGAAARLGAQ 54 Query: 63 CEVLAADVSRSADVKAIAEAAMSHFGRVDVLHNNVGIAEVGGPVETSEESWNRLVAVNQT 122 L ADV+ A +A A A + FGR+D+L NN G+ + +E + + ++R++ V+ Sbjct: 55 HLGLVADVTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLR 114 Query: 123 SLFLTHKYVLPIMEKQRKGAIVNISSMAALR---WIGFPYLGYTATKAAILAFTKNVAMQ 179 + VLP M+ Q+ G+IV ISS++A R +G P+ Y+A KA +L + +A + Sbjct: 115 GTLYMSQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPH--YSAAKAGVLGLARAMARE 172 Query: 180 YAPMGIRANCVLPGMMDTPMIREPLKASYGGDIEEMRAKRNAQCPMGFMGDAWDVAHASL 239 + P GIR NC+ PG++ T + ++ L A E +A+ P+G +G DV + Sbjct: 173 FGPQGIRVNCITPGLISTDINKDILSA-------EKKAEIAQTIPLGRLGGPDDVGGVCV 225 Query: 240 FLASDNARYITGLDMIVDGGL 260 FLASD ++Y TG+ + V+GG+ Sbjct: 226 FLASDLSQYCTGVTLDVNGGM 246 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 127 bits (318), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 88/257 (34%), Positives = 137/257 (53%), Gaps = 17/257 (6%) Query: 8 KAIIVTGAGSVGEGWGNGKAAAVLYAREGGRVLAVDRSIEAALETQEIIRREGGVCEVLA 67 K +++T A G G+A+ L+AREG RV+A D S E I GV E Sbjct: 11 KTVLITAAAQ-----GIGRASTELFAREGARVIATDISKPHLDELAGI----AGV-ETHL 60 Query: 68 ADVSRSADVKAIAEAAMSHFGRVDVLHNNVGIAEVGGPVETSEESWNRLVAVNQTSLFLT 127 DV+ A +KA+ ++ G +DVL N G G +E +++W+ +N ++F T Sbjct: 61 LDVTDDAAIKAL----VAKIGTIDVLFNCAGYVAAGNILECDDKAWDFSFNLNAKAMFHT 116 Query: 128 HKYVLPIMEKQRKGAIVNISSMAALRWIGFPYLGYTATKAAILAFTKNVAMQYAPMGIRA 187 + VLP M ++ G+IVNI+S A+ Y A+KAA++ TK+VA + GIR Sbjct: 117 IRAVLPGMLAKKAGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVAQGIRC 176 Query: 188 NCVLPGMMDTPMIREPLKAS---YGGDIEEMRAKRNAQCPMGFMGDAWDVAHASLFLASD 244 N + PG +++P + + + G EE+RA + PMG +G A +VA +L+LASD Sbjct: 177 NAICPGTIESPSLNQRISTQAKETGKSEEEVRAAFVGRQPMGRIGKAEEVAALALYLASD 236 Query: 245 NARYITGLDMIVDGGLS 261 + + TG ++DGG S Sbjct: 237 ESNFTTGSIHMIDGGWS 253 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 834,488 Number of extensions: 33955 Number of successful extensions: 255 Number of sequences better than 1.0e-08: 46 Number of HSP's gapped: 169 Number of HSP's successfully gapped: 46 Length of query: 266 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 182 Effective length of database: 1,222,130 Effective search space: 222427660 Effective search space used: 222427660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)