BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0049.p (342 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313078|ref|YP_003777170.1| NADP-dependent oxidoreductase [... 608 e-175 gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [H... 68 1e-12 gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [H... 57 3e-09 >gi|300313078|ref|YP_003777170.1| NADP-dependent oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 343 Score = 608 bits (1569), Expect = e-175, Method: Compositional matrix adjust. Identities = 304/338 (89%), Positives = 322/338 (95%) Query: 5 PKTNRRIVLASRPAAAPTAENFRLEEVAIPEVQEGQMLLRGEWLSLDPYMRGRMSDAPSY 64 P+TNRRIVLASRP APTA+NFRLEEVA+P ++EGQ+LLRG+WLSLDPYMRGRMSDAPSY Sbjct: 6 PQTNRRIVLASRPTGAPTADNFRLEEVAVPALEEGQLLLRGQWLSLDPYMRGRMSDAPSY 65 Query: 65 APPVEIDGVMTGGVVSRVVSSRHPDFKEGDLVMAYTGWQEYAISDGSGANKLPADFAHPS 124 APPVEI GVMTGGVVSRV +SRHP+FKEGDLVMAYTGWQEYA+SDG+G NKLPA FAHPS Sbjct: 66 APPVEIGGVMTGGVVSRVEASRHPEFKEGDLVMAYTGWQEYAVSDGTGVNKLPAGFAHPS 125 Query: 125 HALGVLGMPGYTAYAGLIDIGQPRAGETVVVAAATGAVGSLVGQIAKQRGCHVVGIAGGA 184 HALGVLGMPGYTAYAGLIDIGQPR GETVVVAAATGAVGSLVGQIAK RGCHVVGIAGGA Sbjct: 126 HALGVLGMPGYTAYAGLIDIGQPRPGETVVVAAATGAVGSLVGQIAKLRGCHVVGIAGGA 185 Query: 185 QKCESAVKELGFDACIDHRAPDFAEQLKAACPRGIDVYFENVGGAVLDAVIPLLNIHARV 244 QKCE+AVKELGFDACIDHRA DF EQLKAACPRGIDVYFENVGGAVLDAV PLLNIHARV Sbjct: 186 QKCEAAVKELGFDACIDHRAADFPEQLKAACPRGIDVYFENVGGAVLDAVFPLLNIHARV 245 Query: 245 PVCGLIAHYNGGGLATESSAILRRLLTQRIRVQGFIIFDYYVNRPDLYAAFQKEVGTWLK 304 PVCGLIAHYNGG LAT+S+AILRR LTQR+RVQGFIIFDYYVNRPDL+AAFQ+EVG WLK Sbjct: 246 PVCGLIAHYNGGTLATDSAAILRRTLTQRMRVQGFIIFDYYVNRPDLHAAFQQEVGNWLK 305 Query: 305 EGKLHYREDVVQGLEKAPEAFMGLLQGKNFGKLVVKLD 342 +GKLHYREDVVQGLE APEAFMGLLQGKNFGKLVVKLD Sbjct: 306 QGKLHYREDVVQGLENAPEAFMGLLQGKNFGKLVVKLD 343 >gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 67.8 bits (164), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 85/329 (25%), Positives = 147/329 (44%), Gaps = 39/329 (11%) Query: 30 EVAIPEVQEGQMLLRGEWLSLD-PYMRGRMSDAP-----SYAPPVEIDGVMTGGVVSRVV 83 E +PE++ G++L++ ++ P + R + P S P +E+ G + G + Sbjct: 19 ERPVPELKAGEVLIKVHAAGVNRPDVFQRTGNYPVPPGASDLPGLEVAGEIVAGDLG--- 75 Query: 84 SSRHPDFKEGDLVMAYT---GWQEYAISDGSGANKLPADFAHPSHALGVLGMPG--YTAY 138 FK GDLV A G+ E+ + + +P + AL +P +T + Sbjct: 76 ---DSGFKLGDLVCALVQGGGYAEFCAAPLAQCLPVPKGLS----ALEAAALPENYFTVW 128 Query: 139 AGLIDIGQPRAGETVVVAAATGAVGSLVGQIAKQRGCHVVGIAGGAQKCESAVKELGFDA 198 + + D GQ ET++V + +G QIA+ G V AG A+KC A +ELG + Sbjct: 129 SNVFDRGQLSGEETLLVQGGSSGIGVTAIQIARALGHRVFATAGSAEKCR-ACEELGAER 187 Query: 199 CIDHRAPDFAEQLKAAC-PRGIDVYFENVGGAVLDAVIPLLNIHARVPVCGLIAHYNGGG 257 I+++ DF +K RG+DV + VGG L I L R+ IA G Sbjct: 188 AINYKTEDFEAVVKELTGGRGVDVILDMVGGDYLPREIKALADDGRLV---FIAQLGGAK 244 Query: 258 LATESSAILRRLLTQRIRVQGFIIFDYYVN-RPDLYAAFQKEVGTWLKEGK----LHYRE 312 + ++RR LT + G + V + + A ++ V ++ GK +H+R Sbjct: 245 GQLDMGQVMRRRLT----ITGSTLRPRPVAFKAAIAAKLREHVWPLIEAGKIRPVIHHRF 300 Query: 313 DVVQGLEKAPEAFMGLLQGKNFGKLVVKL 341 LE+A +A + + GK++++L Sbjct: 301 P----LEQAAQAHAMMEGSTHIGKIMLEL 325 >gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 56.6 bits (135), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 67/235 (28%), Positives = 99/235 (42%), Gaps = 38/235 (16%) Query: 24 ENFRLEEVAIPEVQEGQMLLRGEWLSLDP---YMR-GRMSDAPSYAPPVE---IDGVMTG 76 E R E+ +PE+Q G++L+R + L+P Y+R G P + P V I G Sbjct: 18 EVLRYEDAPVPELQPGEVLVRVHAVGLNPPDWYLREGYKMLPPEWQPTVAFPLILGTDIS 77 Query: 77 GVVSRVVSSRHPDFKEGDLVMAYT--------GWQEYAISDGSGANKLPADFAHPSHALG 128 GVV V DF GD V + G + YA A+++ SHA Sbjct: 78 GVVEAVADDVK-DFAVGDEVYSMVRFPSGLAGGSRAYAQYVSVAASEIALKPQRISHAQA 136 Query: 129 VLG-MPGYTAYAGLIDIGQ--------------PRAGETVVVAAATGAVGSLVGQIAKQR 173 M TA+ +I++G P +TVVV A G VG Q+AK + Sbjct: 137 AAAPMSLLTAWQFMIELGHDIQNPLQPHRHEPVPLKDKTVVVNGAAGGVGHFALQLAKWK 196 Query: 174 GCHVVGIAGGAQKCESAVKELGFDACIDHRAPDFAEQLKAACPRGIDVYFENVGG 228 G V+ I G E+ +++LG D ID+ + L +D+ + VGG Sbjct: 197 GARVIAIGSGGH--EAFLRDLGADEFIDYTRSAAEDVLS-----DVDLVIDAVGG 244 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.138 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,105,954 Number of extensions: 46354 Number of successful extensions: 152 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 151 Number of HSP's successfully gapped: 3 Length of query: 342 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 256 Effective length of database: 1,212,660 Effective search space: 310440960 Effective search space used: 310440960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)