BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0003.0048.p
         (366 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313077|ref|YP_003777169.1| coenzyme F420-dependent N5,N10-...   645   0.0  
gi|300312181|ref|YP_003776273.1| coenzyme F420-dependent N5,N10-...    66   6e-12

>gi|300313077|ref|YP_003777169.1| coenzyme F420-dependent
           N5,N10-methylene tetrahydromethanopterin reductase
           [Herbaspirillum seropedicae SmR1]
          Length = 336

 Score =  645 bits (1664), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 310/332 (93%), Positives = 326/332 (98%)

Query: 1   MIPFSVLDLSPINQGSTAAQAFNNSKELAQRAEALGYKRYWLAEHHNMPGIASAATSLVI 60
           MIPFSVLDLSPINQGSTAAQAFNNSKELAQRAEALGY RYWLAEHHNMPGIASAAT+LVI
Sbjct: 1   MIPFSVLDLSPINQGSTAAQAFNNSKELAQRAEALGYHRYWLAEHHNMPGIASAATALVI 60

Query: 61  SHVAANTKTIRVGSGGIMLPNHSPLVIAEQFGTLESLYPGRIDLGLGRAPGSDQRTARAL 120
           SHVAANT++IRVGSGGIMLPNHSPLVIAEQFGTLESLYPGRIDLGLGRAPGSDQRTARAL
Sbjct: 61  SHVAANTRSIRVGSGGIMLPNHSPLVIAEQFGTLESLYPGRIDLGLGRAPGSDQRTARAL 120

Query: 121 RRHMGGDTADNFPQDVIELQEYFDEPSDYQNLRAVPGSGLKVPIWLLGSSMFSAQLAAEL 180
           RRHMGGDTAD FPQDV ELQ+YFD+P+DYQ LRAVPG+GLKVPIWLLGSSMFSAQLAAEL
Sbjct: 121 RRHMGGDTADTFPQDVQELQDYFDDPADYQTLRAVPGAGLKVPIWLLGSSMFSAQLAAEL 180

Query: 181 GLPFAFASHFAPGFMKSAVQIYRERFKPSATLKKPYVMLALNVFAADTQREAERLFTSLQ 240
           GLPFAFASHFAPGFMKSAVQIYRERFKPSATLKKPYVMLALNVFAADTQREAERLFTSLQ
Sbjct: 181 GLPFAFASHFAPGFMKSAVQIYRERFKPSATLKKPYVMLALNVFAADTQREAERLFTSLQ 240

Query: 241 QQFLNLIRGTPGQLRPPVDDIDALWQPGEQAHVERSLSCAVVGDRETVEEGLQAFIDEHA 300
           QQF+NLIRGTPGQLRPPVDDIDALWQPGEQAHV RSL+CAVVGDR+ V++GLQAF+DEHA
Sbjct: 241 QQFMNLIRGTPGQLRPPVDDIDALWQPGEQAHVARSLACAVVGDRQAVQDGLQAFMDEHA 300

Query: 301 PDELIITAQIFDHAARLHSFEIAAQAREALKE 332
           PDELIITAQIFDHAARLHSFEIAA+AR+AL+E
Sbjct: 301 PDELIITAQIFDHAARLHSFEIAAEARQALRE 332


>gi|300312181|ref|YP_003776273.1| coenzyme F420-dependent
           N5,N10-methylene tetrahydromethanopterin reductase
           [Herbaspirillum seropedicae SmR1]
          Length = 333

 Score = 65.9 bits (159), Expect = 6e-12,   Method: Compositional matrix adjust.
 Identities = 44/127 (34%), Positives = 64/127 (50%), Gaps = 7/127 (5%)

Query: 13  NQGSTAAQAFNNSKELAQRAEALGYKRYWLAEHHNMPGIASAATSLVISHVAANTKTIRV 72
           N    A  A   + + A  AEALGY   W+AEHH       +A  ++++ +AA T  IR+
Sbjct: 16  NPHGNARDALLQTVDHAVVAEALGYHDVWVAEHHYSSFAIGSALMVLLAQIAARTSRIRL 75

Query: 73  GSGGIMLPNHSPLVIAEQFGTLESLYPGRIDLGLGR-APGSDQRTARALRRHMGGDTADN 131
           G+G  +L  + PL +AE   TL+ L  GR++ G+ R  P  +Q       RH G   AD 
Sbjct: 76  GTGASLLALNDPLRVAEDVATLDLLSNGRMEFGVARGGPFPEQ------YRHAGLSGADQ 129

Query: 132 FPQDVIE 138
               +IE
Sbjct: 130 ARARMIE 136


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.318    0.134    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,134,394
Number of extensions: 46987
Number of successful extensions: 134
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 134
Number of HSP's successfully gapped: 2
Length of query: 366
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 280
Effective length of database: 1,212,660
Effective search space: 339544800
Effective search space used: 339544800
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 131 (55.1 bits)