BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0032.p (498 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313072|ref|YP_003777164.1| high-affinity K+ transport syst... 898 0.0 gi|300311691|ref|YP_003775783.1| cation transport ATPase [Herbas... 108 8e-25 gi|300310937|ref|YP_003775029.1| heavy metal translocating P-typ... 102 9e-23 gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPas... 100 3e-22 gi|300311699|ref|YP_003775791.1| cation transport P-type ATPase ... 77 3e-15 >gi|300313072|ref|YP_003777164.1| high-affinity K+ transport system ATPase [Herbaspirillum seropedicae SmR1] Length = 730 Score = 898 bits (2320), Expect = 0.0, Method: Compositional matrix adjust. Identities = 478/498 (95%), Positives = 490/498 (98%) Query: 1 MIAMVEGARRQKTPNELALTLLLVALTLVFLLVTVTLLPFSIFSVTAAGAGSPVTVTALI 60 MIAMVEGARRQKTPNELALTLLLVALTLVFLLVTVTLLPFSIFSV AAG+GSPV+VTALI Sbjct: 233 MIAMVEGARRQKTPNELALTLLLVALTLVFLLVTVTLLPFSIFSVNAAGSGSPVSVTALI 292 Query: 61 ALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGTITLGN 120 ALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGTITLGN Sbjct: 293 ALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGTITLGN 352 Query: 121 RQAAAFMPAPGVAERELADVAQLASLADETPEGRSIVVLAKNRFNLREREMANLGAEFIP 180 RQAAAFMPAPGV ERELADVAQLASLADETPEGRSIVVLAKNRFNLREREMANLGAEFIP Sbjct: 353 RQAAAFMPAPGVTERELADVAQLASLADETPEGRSIVVLAKNRFNLREREMANLGAEFIP 412 Query: 181 FSAQTRVSGIDIGNRRIRKGAADAIRKLVRSADQRLPAQVESDVEAIARRGGTPLVVIEW 240 FSAQTRVSGID+G+RRIRKGAADAIRKL R+ADQRLPAQVESD+E+IARRGGTPLVVIEW Sbjct: 413 FSAQTRVSGIDLGHRRIRKGAADAIRKLTRAADQRLPAQVESDIESIARRGGTPLVVIEW 472 Query: 241 NNEAAVINQGAPIRILGTVELKDIVKGGIKERFGELRRMGIKTIMITGDNRLTAAAIAAE 300 + EAA+INQGAPIRILGTVELKDIVKGGIKERFGELRRMGIKT+MITGDNRLTAAAIAAE Sbjct: 473 HAEAAMINQGAPIRILGTVELKDIVKGGIKERFGELRRMGIKTVMITGDNRLTAAAIAAE 532 Query: 301 AGVDDFLAEATPEQKLALIRQHQAEGKLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKE 360 AGVDDFLAEATPEQKLALIRQHQAEGKLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKE Sbjct: 533 AGVDDFLAEATPEQKLALIRQHQAEGKLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKE 592 Query: 361 AGNMVDLDSNPTKLIEIVEIGKQMLMTRGSLTTFSIANDVAKYFAIIPAMFVANYPQLGA 420 AGNMVDLDSNPTKLIEIVEIGKQMLMTRGSLTTFSIANDVAKYFAIIPAMFV +YPQLGA Sbjct: 593 AGNMVDLDSNPTKLIEIVEIGKQMLMTRGSLTTFSIANDVAKYFAIIPAMFVGSYPQLGA 652 Query: 421 LNVMRLTSPDSAIMSAVIFNALVIVGLIPLALRGVRYRALGAAVLLRRNLLIYGVGGLLV 480 LNVM L SPDSAIMSAVIFNALVIV LIPLALRGVRYRALGAAVLLRRNLLIYGVGGLLV Sbjct: 653 LNVMHLASPDSAIMSAVIFNALVIVALIPLALRGVRYRALGAAVLLRRNLLIYGVGGLLV 712 Query: 481 PFVGIKLIDLLLTAFGLV 498 PFVGIKLIDLLL+AFGLV Sbjct: 713 PFVGIKLIDLLLSAFGLV 730 >gi|300311691|ref|YP_003775783.1| cation transport ATPase [Herbaspirillum seropedicae SmR1] Length = 853 Score = 108 bits (271), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 115/407 (28%), Positives = 178/407 (43%), Gaps = 62/407 (15%) Query: 60 IALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGTITLG 119 IAL + L+P + +L+ G RM +ANV+ A+E G + VL DKTGT+T Sbjct: 267 IALSMSLLPEELPIILTIFPAIGAWRMARANVLTRRLSAIETLGSISVLCTDKTGTLTEN 326 Query: 120 NRQAAAFMPAP----------GVAERELADVAQLASLADET----PEGRSIVVLA---KN 162 + M A G+ E + + A+LA P ++ LA Sbjct: 327 RMRVVQGMIAGRHMYFDESDIGITSPEWRTLLETAALASSPRPTDPMEKAFHQLAGESAK 386 Query: 163 RFNLREREMANLGAEFIPFSAQTRV-----SGIDIGNRRI-RKGAADAIRKLVRSADQRL 216 RF + L E+ PF+ R G D + KGA +AI L Sbjct: 387 RFA--PPALTQLLQEY-PFTPALRAMTMVWKGDDDAPLLVATKGAPEAIASLCGLT---- 439 Query: 217 PAQVESDVEAIARRGGTPLVVI-----EWNNEAAVINQGAPIRILGTVELKDIVKGGIKE 271 PA+V+ I G L VI W+ +R LG + L D ++ G+ + Sbjct: 440 PAEVQQMQNDIMHLGSHGLRVIAVAACNWSGALPASQAQFGLRYLGLLALADPLRPGVPD 499 Query: 272 RFGELRRMGIKTIMITGDNRLTAAAIAAEAGVD-------------------------DF 306 + I+ IMITGD+ TA IA +AG+ + Sbjct: 500 AIQRCHQAEIRVIMITGDHPSTAQNIARQAGISADQVLLGDEVQAMAEHELARRLRTTNV 559 Query: 307 LAEATPEQKLALIRQHQAEGKLVAMTGDGTNDAPALAQADVAVAMNS-GTQAAKEAGNMV 365 A TP QKL ++ + +G+++AMTGDG NDAPAL A V +AM + GT A+EAG+++ Sbjct: 560 CARITPLQKLRIVAALKDDGEIIAMTGDGVNDAPALKAAHVGIAMGARGTDVAREAGSLI 619 Query: 366 DLDSNPTKLIEIVEIGKQML-MTRGSLTTFSIANDVAKYFAIIPAMF 411 LD N ++E + +G+++ RG++ + A++P +F Sbjct: 620 LLDDNFASIVEGILMGRRIFSRMRGAMIYVLAMHMPIAGMALLPVVF 666 >gi|300310937|ref|YP_003775029.1| heavy metal translocating P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 102 bits (253), Expect = 9e-23, Method: Compositional matrix adjust. Identities = 91/333 (27%), Positives = 155/333 (46%), Gaps = 37/333 (11%) Query: 60 IALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGTITLG 119 +A++V P +G +AG + ++ A+E A V+ + DKTGT+TLG Sbjct: 376 VAVMVIACPCALGLATPTAIMAGTGVAARHGILIKDAEALEIAHRVNAVAFDKTGTLTLG 435 Query: 120 NRQAAAFMPAPGVAERELADVAQLASLADETPEGRSIVVLAKNRFNLRE-----REMANL 174 + + P EL +A E P GR++ A R +A L Sbjct: 436 HPRLLELEATPSTQPDELLALAAALQAHSEHPLGRAVREAAAQSAQAARQAEQVRSVAGL 495 Query: 175 GAEFIPFSAQTRVSGIDIGNRRIRKGAADAIRKLVRSADQRLPAQVESDVEAIARRGGTP 234 G E + + R++ G++ +++L A PA +++ E + +G T Sbjct: 496 GIEGV------------VEGRQLMLGSSRWMQQLQAHA----PA-LQARAEQLQAQGRT- 537 Query: 235 LVVIEW---NNEAAVINQGAPIRILGTVELKDIVKGGIKERFGELRRMGIKTIMITGDNR 291 + W N++ V +LG + D +K G + L RMG+++ M++GDN Sbjct: 538 ---LSWLARRNDSGV-------EVLGLLAFGDQIKPGAQAAIATLHRMGVRSAMLSGDNL 587 Query: 292 LTAAAIAAEAGVDDFLAEATPEQKL-ALIRQHQAEGKLVAMTGDGTNDAPALAQADVAVA 350 A +A+E G+ + AE P K A+ Q ++VAM GDG NDAPALA ADV +A Sbjct: 588 GAAQQVASELGIQEVRAEVLPADKAEAVTALRQGGAQVVAMVGDGVNDAPALAAADVGMA 647 Query: 351 MNSGTQAAKEAGNMVDLDSNPTKLIEIVEIGKQ 383 M++GT A +A + + +P + + ++I ++ Sbjct: 648 MSTGTDVAMQAAGVTLMHGDPGLVADAIDISRR 680 >gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 778 Score = 100 bits (249), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 100/335 (29%), Positives = 158/335 (47%), Gaps = 45/335 (13%) Query: 56 VTALIALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGT 115 V A LV P TI V+G++ + ++ G +E + L LDKTGT Sbjct: 420 VIACPCALVISTPVTI--------VSGLASAARHGILVKGGVYLEQGRQLRALALDKTGT 471 Query: 116 ITLGNRQAAAFMPAPGVAERELADVAQLA-SLADETPEGRSIVVLAKNRFNLRE-REMAN 173 +T G + + A+++ + QLA SLA + S + A+ E E A Sbjct: 472 LTFGKPRVTDVVSLQAQADQQA--LLQLAVSLAARSDHPVSRAIAAQEEIATVEVSEFAA 529 Query: 174 LGAEFIPFSAQTRVSG--IDIGNRRIRKGAADAIRKLVRSADQRLPAQVESDVEAIARRG 231 L + Q R++G + +GN R LV Q P ++E+ ++A ++G Sbjct: 530 LAGRGV----QGRINGQLLHLGNHR-----------LVHELGQCSP-ELEARLQAFEKQG 573 Query: 232 GTPLVVIEWNNEAAVINQGAPIRILGTVELKDIVKGGIKERFGELRRMGIKTIMITGDNR 291 T V+ G P + V + D V+ ++ ELR +G++ IM+TGDN Sbjct: 574 KTTTVLCL---------DGQPQLL---VAVADTVRPSSRQAVAELRALGVEVIMLTGDNS 621 Query: 292 LTAAAIAAEAGVDDFLAEATPEQKLALIRQ-HQAEG-KLVAMTGDGTNDAPALAQADVAV 349 TA AIAA+ G+ D + PE KL I + Q G + V M GDG NDAPALA+AD+ Sbjct: 622 HTAQAIAAQTGISDARGDQLPEDKLKAIEELSQRHGVRGVGMVGDGINDAPALARADIGF 681 Query: 350 AMN-SGTQAAKEAGNMVDLDSNPTKLIEIVEIGKQ 383 AM +GT A E ++ +D + K+ + + + ++ Sbjct: 682 AMGAAGTDTALETADVALMDDDLRKIPDFIRLSRK 716 >gi|300311699|ref|YP_003775791.1| cation transport P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 734 Score = 77.0 bits (188), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 89/300 (29%), Positives = 125/300 (41%), Gaps = 44/300 (14%) Query: 54 VTVTALIALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKT 113 V V A L+ +P I V MSR Q V+ G A+E V L DKT Sbjct: 231 VVVVATPCPLILAVPVAI--------VCAMSRCAQRGVLIKHGGALEKLAQVRTLFFDKT 282 Query: 114 GTITLGNRQAAAFMPAPGVAERELADVAQLASLADETPE--GRSIVVLAKNR---FNLRE 168 GT+T G + + P + EL +A ASL + +++V A R ++ + Sbjct: 283 GTLTAGKARLMNMVTTPSIQADELLRLA--ASLEQMSNHVIAQAVVSAAIERALVLSIPD 340 Query: 169 REMANLGAEFIPFSAQTRVSGIDIGNRRIRKGAADAIRKLVRSADQRLPAQVESDVEAIA 228 + GA RV G + +R GA + P + + Sbjct: 341 QVQEQPGAGL-----SGRVDG-----KLVRVGAMQYV-----IGPSAAPPWTSGLRDRLK 385 Query: 229 RRGGTPLVVIEWNNEAAVINQGAPIRILGTVELKDIVKGGIKERFGELRRMGIKTI-MIT 287 GG + V AV Q + G + L D ++ +LR +G K I M+T Sbjct: 386 YEGGAGVCV-------AVDGQ-----LCGVLYLADDIRLDTPRALRQLRALGAKRIVMLT 433 Query: 288 GDNRLTAAAIAAEAGVDDFLAEATPEQKLALIRQHQAEGKLVAMTGDGTNDAPALAQADV 347 GD R A I + G DD + E +P KLA I+Q EG + M GDG NDAPALA AD+ Sbjct: 434 GDRRDVAMTIGSSLGFDDVMPEQSPASKLAAIKQASREG-VTMMVGDGINDAPALAAADI 492 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,363,636 Number of extensions: 55052 Number of successful extensions: 318 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 306 Number of HSP's successfully gapped: 7 Length of query: 498 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 409 Effective length of database: 1,198,455 Effective search space: 490168095 Effective search space used: 490168095 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)