BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0022.p (264 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspiri... 442 e-125 gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 134 6e-33 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 121 5e-29 gi|300312380|ref|YP_003776472.1| enoyl-CoA hydratase/carnithine ... 118 4e-28 gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 102 2e-23 >gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 442 bits (1137), Expect = e-125, Method: Compositional matrix adjust. Identities = 216/260 (83%), Positives = 229/260 (88%) Query: 1 MQDASQDLLLRHDAQGITTLTLNRPAQFNALSEAMLAALQAQLDALAQDPQVRCVILAAN 60 M+D D+L R D QGITTLTLNRP FNALSE ML ALQAQLD + D VRCVILAAN Sbjct: 1 MKDHHPDILEREDQQGITTLTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAAN 60 Query: 61 GRAFCAGHDLREMRATPEQAYYQQLFDRCSRLMQSLRALPVPVIAKVQGLATAAGCQLVA 120 G+AFCAGHDLR+M+ATP+Q YY+ LF RCS LMQSLR LPVPVIAKVQGLATAAGCQLVA Sbjct: 61 GKAFCAGHDLRQMQATPQQTYYEALFHRCSHLMQSLRELPVPVIAKVQGLATAAGCQLVA 120 Query: 121 ACDLAVASEGARFAVSGINVGLFCSTPAVALTRNLPIKRAFEMLVTGKFIDADTAAQWGL 180 CDLA+A+E ARFAVSGINVGLFCSTPAVALTRNLPIKRAFEMLVTGKFIDA TAA WGL Sbjct: 121 TCDLAIAAESARFAVSGINVGLFCSTPAVALTRNLPIKRAFEMLVTGKFIDAATAAAWGL 180 Query: 181 INQAVRADELDAATEALAREICSKPAISIARGKAMVYRQQMMALPDAYAHAAEVMACNIM 240 INQAV ELD+AT+ALAREICSKPAISIARGKAMVYRQQMMALPDAYAHAAEVMACNIM Sbjct: 181 INQAVPEQELDSATQALAREICSKPAISIARGKAMVYRQQMMALPDAYAHAAEVMACNIM 240 Query: 241 DAEAMEGIQAFLEKRPPNWA 260 D EA EGI AFL+KR PNWA Sbjct: 241 DPEAQEGITAFLDKRKPNWA 260 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 134 bits (338), Expect = 6e-33, Method: Compositional matrix adjust. Identities = 89/258 (34%), Positives = 127/258 (49%), Gaps = 4/258 (1%) Query: 9 LLRHDAQGITTLTLNRPAQFNALSEAMLAALQAQLDALAQDPQVRCVILAANGRAFCAGH 68 +L + GI +TLNRP + N+ + AM L+ + L DP VR ++L GR FCAG Sbjct: 6 ILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRGFCAGQ 65 Query: 69 DL--REMRATPEQAYYQQLFDRC-SRLMQSLRALPVPVIAKVQGLATAAGCQLVAACDLA 125 DL R ++ + + D+ L++SLRALP PVI V G+A AG L ACD+ Sbjct: 66 DLGDRAVKPGDDGVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPLACDIV 125 Query: 126 VASEGARFAVSGINVGLFCSTPAVA-LTRNLPIKRAFEMLVTGKFIDADTAAQWGLINQA 184 +A+ A F +GL T L R + RA M + G+ I A+ A +WGLI + Sbjct: 126 LAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWGLIWKC 185 Query: 185 VRADELDAATEALAREICSKPAISIARGKAMVYRQQMMALPDAYAHAAEVMACNIMDAEA 244 V D+L A+A P +A KA +Y LP+ + M + Sbjct: 186 VDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALGRSRDY 245 Query: 245 MEGIQAFLEKRPPNWAGR 262 EG+ AFLEKR P + G+ Sbjct: 246 REGVTAFLEKRAPQFTGQ 263 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 121 bits (304), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 90/265 (33%), Positives = 132/265 (49%), Gaps = 6/265 (2%) Query: 1 MQD--ASQDLLLRHDAQGITTLTLNRPAQFNALSEAMLAALQAQLDALAQDPQVRCVILA 58 MQD A +DLL + + LTLNRPA NAL L + A L +D VR V+++ Sbjct: 1 MQDTVAYEDLLAARHGEHVLHLTLNRPALRNALRNQSLREIVAALARAEEDDSVRVVVIS 60 Query: 59 ANGRAFCAGHDLREMRATPEQAYYQQLFDRCSRLMQSLRALPVPVIAKVQGLATAAGCQL 118 N +AF AG DL EM + A QL D ++ +++ P P++A V G A AGC+L Sbjct: 61 GNEKAFAAGADLNEM--IHKDAIATQL-DVRAQYWRAIARFPKPILAAVNGYALGAGCEL 117 Query: 119 VAACDLAVASEGARFAVSGINVGLFCSTPAVA-LTRNLPIKRAFEMLVTGKFIDADTAAQ 177 + D+A+A+ GA+ INVG L R + A +M+++G+FI AD A Q Sbjct: 118 LMHADIAIAARGAKIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQ 177 Query: 178 WGLINQAVRADELDAATEALAREICSKPAISIARGKAMVYRQQMMALPDAYAHAAEVMAC 237 GL+ + V D T ALA I K +++ K + + + L + + Sbjct: 178 AGLVAEVVDDDATLERTLALAHSIAQKSPLAVRLAKEAMLQSFELGLEAGLLFERKSFSL 237 Query: 238 NIMDAEAMEGIQAFLEKRPPNWAGR 262 A+ EGI AF EKR ++GR Sbjct: 238 MAASADRQEGIAAFQEKRAAVFSGR 262 >gi|300312380|ref|YP_003776472.1| enoyl-CoA hydratase/carnithine racemase [Herbaspirillum seropedicae SmR1] Length = 269 Score = 118 bits (296), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 79/248 (31%), Positives = 128/248 (51%), Gaps = 3/248 (1%) Query: 15 QGITTLTLNRPAQFNALSEAMLAALQAQLDALAQDPQVRCVILAANGRAFCAGHDLREMR 74 +G+ T+TLNRP NA + ++A L L D + ++LA+ G++F AG DL MR Sbjct: 12 RGLATITLNRPEIHNAFDDQLIAHLIDLLTQARDDTRTCLLLLASTGKSFSAGADLAWMR 71 Query: 75 ATPEQAYYQQLFD--RCSRLMQSLRALPVPVIAKVQGLATAAGCQLVAACDLAVASEGAR 132 + + L D + + LM++L + P P +AKVQG G LV+ CD+ VAS+ A Sbjct: 72 RMADASREDNLRDARKLALLMETLNSFPTPTLAKVQGAVMGGGVGLVSCCDIVVASDAAF 131 Query: 133 FAVSGINVGLFCSTPAVALTRNLPIKRAFEMLVTGKFIDADTAAQWGLINQAVRADELDA 192 FA+S + +GL + + + + + +A V+ + A AA GL+++ DELDA Sbjct: 132 FALSEVKLGLAPAAISPYVIAKMGVSQARRYFVSAERFPAARAAAMGLVHEVAPPDELDA 191 Query: 193 ATEALAREICSKPAISIARGKAMV-YRQQMMALPDAYAHAAEVMACNIMDAEAMEGIQAF 251 T L + + ++ K +V + P+ + A V+A AE EG++AF Sbjct: 192 RTAQLIDTLLANGPQAMRAAKQLVQVANPLQVTPELSEYTAGVIADLRASAEGREGLRAF 251 Query: 252 LEKRPPNW 259 L+K P W Sbjct: 252 LDKDAPAW 259 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 102 bits (255), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 74/250 (29%), Positives = 120/250 (48%), Gaps = 11/250 (4%) Query: 12 HDAQGITTLTLNRPAQFNALSEAMLAALQAQLDALAQDPQVRCVILAANGRAFCAGHDLR 71 H+ G+ + L+RP NALS+ ++ L L A ++ C+I+ + +AF AG D+ Sbjct: 11 HEKVGL--IRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGSEKAFAAGADIS 68 Query: 72 EMRATPEQAYYQQLFDRCSRLMQSLRALPVPVIAKVQGLATAAGCQLVAACDLAVASEGA 131 M ++ F +R ++LR + PVIA V G A GC+L CD +A++ A Sbjct: 69 AMAGYDYMDVFKGEF--ITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDFIIAADNA 126 Query: 132 RFAVSGINVGLFCSTPAVALTRNLP----IKRAFEMLVTGKFIDADTAAQWGLINQAVRA 187 +F I +G+ P T+ LP +A ++ +TG+ +DA+ A + GL+++ V A Sbjct: 127 KFGQPEIKLGI---VPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAERAGLVSRIVAA 183 Query: 188 DELDAATEALAREICSKPAISIARGKAMVYRQQMMALPDAYAHAAEVMACNIMDAEAMEG 247 D+L A A I P K V R L + + + + EG Sbjct: 184 DKLLEEAMAAAIIIAEMPRQVAMMVKDSVNRAYETTLSEGMKYERALFYSCFATEDQKEG 243 Query: 248 IQAFLEKRPP 257 ++AFLEKR P Sbjct: 244 MKAFLEKRQP 253 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.132 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 682,547 Number of extensions: 22738 Number of successful extensions: 153 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 139 Number of HSP's successfully gapped: 10 Length of query: 264 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 181 Effective length of database: 1,226,865 Effective search space: 222062565 Effective search space used: 222062565 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)