BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0005.p (465 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313056|ref|YP_003777148.1| major facilitator superfamily p... 891 0.0 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 192 6e-50 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 192 7e-50 gi|300311543|ref|YP_003775635.1| major facilitator superfamily p... 166 5e-42 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 165 8e-42 >gi|300313056|ref|YP_003777148.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 465 Score = 891 bits (2302), Expect = 0.0, Method: Compositional matrix adjust. Identities = 439/465 (94%), Positives = 455/465 (97%) Query: 1 MQSSHSSDHSQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASL 60 MQS+ S+ H QQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASL Sbjct: 1 MQSTQSNAHHQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASL 60 Query: 61 ATYGVGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPAL 120 ATYGVGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPAL Sbjct: 61 ATYGVGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPAL 120 Query: 121 LVIMRLAQGFAVAGEISGASSMILEHSPFGRRGYYSSFTLQGVQAGQILAAAVFLPLAHY 180 LVIMRLAQGFAVAGEISGASSMILEHSPFGRRGY+SSFTLQGVQAGQILAAAVFLPLAHY Sbjct: 121 LVIMRLAQGFAVAGEISGASSMILEHSPFGRRGYFSSFTLQGVQAGQILAAAVFLPLAHY 180 Query: 181 MPAEQFNVWGWRIPFLLSFLVIIAGWIIRRTVEETPLFKAADKNELKRRSPVVEAFRDSS 240 MPAEQFNVWGWRIPFLLSFLVIIAGW+IRRTV+ETP+FKAADK ELKRRSPVV+AFRD S Sbjct: 181 MPAEQFNVWGWRIPFLLSFLVIIAGWVIRRTVDETPVFKAADKEELKRRSPVVDAFRDCS 240 Query: 241 PNMLRVVCMALMNVIPVVATIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIVAVLVIPYVG 300 PNMLRVVCMALMNVIPVVATIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIVAVLVIPYVG Sbjct: 241 PNMLRVVCMALMNVIPVVATIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIVAVLVIPYVG 300 Query: 301 KLSDRIGRRPPIIVGSLLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSF 360 KLSDRIGRRPPIIVGSLLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSF Sbjct: 301 KLSDRIGRRPPIIVGSLLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSF 360 Query: 361 YPELFPARSRVSAMAIAQNVGTAITAMLPALFTAVAPPGSSNIWLTVGSIAFGITIVSAL 420 YPELFPAR+RVSAMAIAQN+GTAITAMLPALFTAVAPPGSSNIWLTVGSIAF ITIVSAL Sbjct: 361 YPELFPARARVSAMAIAQNLGTAITAMLPALFTAVAPPGSSNIWLTVGSIAFAITIVSAL 420 Query: 421 AALSARETYRIPLEKLGERDAQPMPLNEYERLRAQADLEGRYAKS 465 AALSARETYRIPLEKL + +AQP+P NEY+RLRA+A++ GRYAKS Sbjct: 421 AALSARETYRIPLEKLDDPNAQPLPRNEYDRLRAEAEVAGRYAKS 465 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 192 bits (487), Expect = 6e-50, Method: Compositional matrix adjust. Identities = 131/424 (30%), Positives = 216/424 (50%), Gaps = 17/424 (4%) Query: 12 QSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLATYGVGYVARP 71 Q+++A S IG+ALE++D IY A +I Q FFP GD + +++ + AT+GV + RP Sbjct: 16 QARRAIISSSIGNALEWFDILIYGAFAVVIAKQ-FFPTGDDSVSLLLTFATFGVSFFMRP 74 Query: 72 IGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLVIMRLAQGFA 131 +GA VLG D GRK L+ + LM T + +P+Y +GL APA + + ++ QGF+ Sbjct: 75 LGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMIQGFS 134 Query: 132 VAGEISGASSMILEHSPFGRRGYYSSFTLQGVQAGQILAAAVFLPLAHYMPAEQFNVWGW 191 GE +++ ++EH+P RRG++SS+ + +LAA L + + EQ WGW Sbjct: 135 AGGEFGSSTAFLVEHAPH-RRGFFSSWQVASQGISLLLAALFGAVLNNMLTPEQLASWGW 193 Query: 192 RIPFLLSFLVIIAGWIIRRTVEETPLFKAADKNELKRRSPVVEAFRDSSPNMLRVVCMAL 251 R+PF+ L+ AG IRR ++E P FK + + K +P+ + F +L + Sbjct: 194 RVPFIFGLLIAPAGIYIRRHLDEAPEFKESSE---KTNAPLRDTFAHQKMRLLIGAGSVI 250 Query: 252 MNVIPVVATIFGAAYAVQ----PAYGIGFAKDVYLWIPVVGNIVAVLVIPYVGKLSDRIG 307 M + V +++ YAV+ PA+ A V I +G+ P VG LSD+IG Sbjct: 251 MATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGS-------PLVGALSDKIG 303 Query: 308 RRPPIIVGSLLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSFYPELFPA 367 R P +I S L + + ++ L ++ + GV+ Y A P+ ++FP Sbjct: 304 RTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPV 363 Query: 368 RSRVSAMAIAQNVGTAITAMLPALFTAVAPPGSSNIWLTVGSIAFGITIVSALAALSARE 427 +R + M++A N+ I L + + + FG ++S +A+LSAR Sbjct: 364 ATRGTGMSLAYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFG-AVLSLIASLSARL 422 Query: 428 TYRI 431 R+ Sbjct: 423 VLRL 426 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 192 bits (487), Expect = 7e-50, Method: Compositional matrix adjust. Identities = 128/401 (31%), Positives = 214/401 (53%), Gaps = 16/401 (3%) Query: 7 SDHSQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLATYGVG 66 S +QQ ++A + IG+ LE++DF +Y+ A +I +LFFP G+ T+++ ++AT+GVG Sbjct: 13 SVSNQQRRRAIVATVIGNGLEWFDFTVYSFFA-VIIAKLFFPTGNDLTSLLLAVATFGVG 71 Query: 67 YVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLVIMRL 126 + RP+G +LG D GRK L + LM T +GL PTY+ +GL+AP ++V+ RL Sbjct: 72 FFMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARL 131 Query: 127 AQGFAVAGEISGASSMILEHSPFGRRGYYSSFTLQGVQAGQILAAAVFLPLAHYMPAEQF 186 QGF+ GE+ A++ + E++P R YYSS+ + +L AAV + + A Sbjct: 132 LQGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAAL 191 Query: 187 NVWGWRIPFLLSFLVIIAGWIIRRTVEETPLF-KAADKNELKRRSPVVEAFRDSSPNMLR 245 WGWR+PFLL ++ G+ IR +EETP F + DK + + + R++S + Sbjct: 192 ASWGWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTDSPLKEVIRNFPRETSASFSM 251 Query: 246 VVCMALMNVIPVVATIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIVAVLVIPYVGKLSDR 305 V+ L V V + Y+V+ + + +VG + +++ P VG+L+DR Sbjct: 252 VI---LWTVCTYVLLFYMPTYSVKV---LKLPQTTGFIAGMVGGLCIMVLAPIVGRLADR 305 Query: 306 IGRRPPIIVGSLLAGLFA---FAYLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSFYP 362 IGRRP + + L + A FAY+ + + L F L++G + Y + + Sbjct: 306 IGRRPLLSGSAALILMLAYPMFAYINSAPSLSTLLIFQ---LVFGTLIAAYTGPILAAFS 362 Query: 363 ELFPARSRVSAMAIAQNVGTAITAMLPALFTA--VAPPGSS 401 ELFPA+ + +++A N+ I + F +A GS+ Sbjct: 363 ELFPAKVLSTGLSVAYNLAVTIFGGFASFFITWLIAATGST 403 >gi|300311543|ref|YP_003775635.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 553 Score = 166 bits (419), Expect = 5e-42, Method: Compositional matrix adjust. Identities = 110/320 (34%), Positives = 171/320 (53%), Gaps = 14/320 (4%) Query: 12 QSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLATYGVGYVARP 71 + KK + +G+ E+YDF++Y + A++I Q FF DP TA I +L + G++ RP Sbjct: 16 EEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQ-FFAGTDPNTAFIFALLAFAAGFIVRP 74 Query: 72 IGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLVIMRLAQGFA 131 GA V G LGD GRK L + +MG ST VG+LP Y+ +G+ AP +L+I+R+ QG A Sbjct: 75 FGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITAPIILIILRILQGLA 134 Query: 132 VAGEISGASSMILEHSPFGRRGYYSSFTLQGVQAGQILAAAVFLPLAHYMPAEQFNVWGW 191 + GE GA++ + EH+P GRRG ++++ G L+ V L + + F WGW Sbjct: 135 LGGEYGGAATYVAEHAPEGRRGSFTAWIQTTATMGLFLSLLVILGVRTAVGEAAFADWGW 194 Query: 192 RIPFLLSFLVIIAGWIIRRTVEETPLFKAADKNELKRRSPVVEAFRDSSPNMLRVVCMAL 251 RIPFL+S L++ IR ++ E+P F ++P+ EAF L++V +AL Sbjct: 195 RIPFLVSILLLAISVWIRMSMNESPAFLKMKAEGKTSKAPLTEAF--GQWKNLKLVILAL 252 Query: 252 MNVIPVVATIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIVAVLVIP-------YVGKLSD 304 + + A ++ Y Q Y + F NI+ + + G LSD Sbjct: 253 VGLTAGQAVVW---YTGQ-FYALFFLTQTLKVDGPTANILIAAALLLATPFFLFFGSLSD 308 Query: 305 RIGRRPPIIVGSLLAGLFAF 324 +IGR+P I+ G L+A + F Sbjct: 309 KIGRKPIILGGCLIAAVTYF 328 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 165 bits (417), Expect = 8e-42, Method: Compositional matrix adjust. Identities = 110/374 (29%), Positives = 192/374 (51%), Gaps = 13/374 (3%) Query: 12 QSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLATYGVGYVARP 71 ++ + +G+ALE+YDF +Y +++I +LFFP + T+++ +LAT+G+G+ RP Sbjct: 6 NKQRQVIAAIVGNALEWYDFIVYGFFSAII-ARLFFPADNEYTSLLVALATFGIGFFMRP 64 Query: 72 IGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLVIMRLAQGFA 131 +G +LG D GRK + + LM + + PTY +G+ AP ++V+ R+ QGFA Sbjct: 65 VGGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFA 124 Query: 132 VAGEISGASSMILEHSPFGRRGYYSSFTLQGVQAGQILAAAVFLPLAHYMPAEQFNVWGW 191 GE S +++ ++E +P +RG Y S+ L G A + + + E + WGW Sbjct: 125 TGGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDSWGW 184 Query: 192 RIPFLLSFLVIIAGWIIRRTVEETPLF-KAADKNELKRRSPVVEAFRDSSPNMLRVVCMA 250 R+PF+L L+ G IRR + ET F ++A+K +P+ D LR V ++ Sbjct: 185 RLPFVLGLLIGPVGLWIRRHMHETDDFLESANK---PNAAPI--KLLDVVKTNLRGVLVS 239 Query: 251 LMNVIPVVATIFGAAYAVQPAYG---IGFAKDVYLWIPVVGNIVAVLVIPYVGKLSDRIG 307 + VI F P + +G D + ++ + VIP+ G LSDR+G Sbjct: 240 MGQVINGTVA-FYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLG 298 Query: 308 RRPPIIVGSLLAGLFAFA-YLYAISIRNVELAFVMSILMWGVVYQGYNAVFPSFYPELFP 366 RR ++ SL L + +++ + ++ VM +L+ V+ + + P+ E FP Sbjct: 299 RRTVLLYSSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPM-PTALSEQFP 357 Query: 367 ARSRVSAMAIAQNV 380 R R + +A+A N+ Sbjct: 358 TRVRSTGLAVAYNL 371 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.139 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,407,996 Number of extensions: 59892 Number of successful extensions: 347 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 313 Number of HSP's successfully gapped: 14 Length of query: 465 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 376 Effective length of database: 1,198,455 Effective search space: 450619080 Effective search space used: 450619080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)