BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0258.p (379 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309898|ref|YP_003773990.1| major facilitator superfamily p... 106 4e-24 gi|300309383|ref|YP_003773475.1| permease [Herbaspirillum serope... 93 3e-20 gi|300309392|ref|YP_003773484.1| major facilitator superfamily (... 60 3e-10 >gi|300309898|ref|YP_003773990.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 392 Score = 106 bits (264), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 109/363 (30%), Positives = 167/363 (46%), Gaps = 6/363 (1%) Query: 1 MLAVAAGSAIASNYAMQPALPEIAADFGVEVASLTTVISGAIAGYLIGLVLLVPLVDRLS 60 + A+A+G ++A+ Y QP L ++ DF + A++ V++ G + L+LLVPL D++ Sbjct: 15 LFAIASGLSVANVYYAQPLLDALSDDFKISQAAVGGVVTATQLGCALALLLLVPLGDKVP 74 Query: 61 PRVLIPGQFLALALSLALAARAASPDALIGCFVLIGATTTVAAQS-SAIVGKLADASRRA 119 ++L+ Q LAL +L A S L+ + +G T Q A A R Sbjct: 75 RKMLMGIQLLALVCALVTVGLARSSYVLLAGMLAVGLLGTAMTQGLIAYAASAAGEDERG 134 Query: 120 LSMAAISAGISAGILLSRFIGGLLVQWLGWRGALLVLAALTAVCGLCVFPLLPAQRPVGH 179 + A +G+ G+LLSR G + GWRG L A + ++ LPA PV Sbjct: 135 RVVGAAQSGVFIGLLLSRVFSGAVSDVTGWRGVYLCAAIMMLCLAWPLWKRLPAI-PVQA 193 Query: 180 IG--YFSTLRALPSLLRQSRELRLRICAGMLWFFAFNLIWVGLVVRLAAPPYHLNAASIG 237 G Y + ++++ LLR + L++R ML F AFN+ W LV+ L+A PYH + IG Sbjct: 194 DGLRYLTLIKSMWCLLRDDKVLQVRGVLAMLMFAAFNVFWSALVLPLSASPYHFSHTVIG 253 Query: 238 LYSLAGVTGLAMTRIAGGLADRFGNCSVMVCGLLCASLAASTLGVVLDHVPATLVALAIF 297 + L GV G AG +D+ L LA L ++ + A + + + Sbjct: 254 AFGLVGVIGALAASKAGRWSDQGHAQRTSAVSLAVLLLAWWPLSLMESSLWALVAGIILL 313 Query: 298 DAGCFAAQVANQAGVVAIDPLRSGVLNSAYLFFY-YGAGAAGTAIAGSLALRGSWQTTVL 356 D G A V NQ+ + P L Y+ FY G+G + A RG WQ Sbjct: 314 DLGGQALHVTNQSLIFRSSPQAHSRLVGVYMLFYAVGSGLGAISTTAIYAARG-WQGVCF 372 Query: 357 LAA 359 L A Sbjct: 373 LGA 375 >gi|300309383|ref|YP_003773475.1| permease [Herbaspirillum seropedicae SmR1] Length = 412 Score = 93.2 bits (230), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 114/348 (32%), Positives = 160/348 (45%), Gaps = 8/348 (2%) Query: 6 AGSAIASNYAMQPALPEIAADFGVEVASLTTVISGAIAGYLIGLVLLVPLVDRLSPRVLI 65 AG A+AS Y QP L +A D G ++ V GY +G++LL PL DR R +I Sbjct: 35 AGLAVASLYYSQPMLGVLADDIGASERAVGMVPMSTQLGYALGILLLAPLGDRYDRRRII 94 Query: 66 PGQFLALALSLALAARAASPDALIGCFVLIGATTTVAAQSSAIVGKLADASRRALSMAAI 125 + L +L LA A+ AL+ + +G T T+A LA R + + Sbjct: 95 LIKAAILTAALLLAGAASGVGALLAASLAVGLTATLAQDIVPAAASLAPEQSRGRVVGTV 154 Query: 126 SAGISAGILLSRFIGGLLVQWLGWRGALLVLAALTAVCGLCVFPLLPAQRPVGHIGYFST 185 G+ GILLSR + G + + GWR + A A+ GL + LP P H+ Y + Sbjct: 155 MTGLLLGILLSRVVSGFVAEHFGWRAMFVAAAVSIALIGLAAWRGLPRFHPTTHLSYGAL 214 Query: 186 LRALPSLLRQSRELRLRICAGMLWFFAFNLIWVGLVVRLAAPPYHLNAASIGLYSLAGVT 245 + +L +L ++ LR A L F+ W L V L P+HL +A+ G + LAG Sbjct: 215 MASLATLFKKHGALRRAALAQGLLSVGFSAFWSTLAVMLHGAPFHLGSAAAGAFGLAGAA 274 Query: 246 GLAMTRIAGGLADRFGN--CSVMVCGLLCASLAASTLGVVLDHVPATLVALAI----FDA 299 G +AG ++D+ G + + GL S AA L LV LA+ FD Sbjct: 275 GALAAPLAGRISDKKGPELVTRLGVGLAIVSFAAMALSPWFS-TQGQLVLLAVAAVGFDL 333 Query: 300 GCFAAQVANQAGVVAIDPLRSGVLNSAYLFFYYGAGAAGTAIAGSLAL 347 G A VA+Q V +I+P LN A LF G A A GSL L Sbjct: 334 GVQATLVAHQTIVYSIEPGARSRLN-AILFVGMFIGMAAGAALGSLVL 380 >gi|300309392|ref|YP_003773484.1| major facilitator superfamily (MFS) transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 423 Score = 60.1 bits (144), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 100/356 (28%), Positives = 153/356 (42%), Gaps = 33/356 (9%) Query: 14 YAMQPALPEIAADFGVEVASLTTVISGAIAGYLIGLVLLVPLVDRLSPRVLIPGQFLALA 73 Y +QP LP A +FG+ AS + +S + + +VL L +R R L+ LA A Sbjct: 58 YCVQPLLPIFAQEFGIGAASASLALSLSTGLLAVSIVLAGMLSERYGRRELMFAS-LATA 116 Query: 74 LSLALAARAASPDA--LIGCFVLIG-ATTTVAAQSSAIVGKLADASRRALSMAAISAGIS 130 LAA A PD L+ L G A V A + A + + DA+ +M G + Sbjct: 117 ACCNLAAGVA-PDWHLLLLARTLEGIALGGVPAVAMAYLAEEIDAAGLGFAMGLYVGGNA 175 Query: 131 AGILLSRFIGGLLVQWLGWRGALLVLAALTAVCGLCVFPLLPAQRPVGHIGYFSTLRALP 190 G + R +L + LGWR +L++ AL + +LPA R H S++R Sbjct: 176 FGGMAGRVAMSVLAEHLGWRAGMLIIGALDLALAVAFVMMLPASR---HFVRNSSMRLAD 232 Query: 191 --SLLR-QSRELRLRICAGMLWFFAFNLIWVGLVV--------RLAAPPYHLNAASIGLY 239 +L R R RL +C FA + +G+ V RL +PP+ L+A GL Sbjct: 233 HFALWRGHLRHARLPLC------FAIAALNMGVFVTSYNYAGFRLMSPPFSLSATVTGLI 286 Query: 240 SLAGVTGLAMTRIAGGLADRFGNCSVMVCGLLCASLAASTLGVVLDHVPATLV---ALAI 296 A + G+ + AG LADR+G L+ A + S LG++L + ++ + + Sbjct: 287 FSAYLFGMLSSSWAGALADRYGRAP-----LVAAGIGVSCLGLLLTCTSSLVLVIGGIVL 341 Query: 297 FDAGCFAAQVANQAGVVAIDPLRSGVLNSAYLFFYYGAGAAGTAIAGSLALRGSWQ 352 G F A + V + G S YL YY G+ ++ G W Sbjct: 342 LTIGFFTTHSAASSWVGRMAREGKGHAASLYLLAYYLGGSILGSVGGWFWEHAGWN 397 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.138 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 828,881 Number of extensions: 27862 Number of successful extensions: 240 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 234 Number of HSP's successfully gapped: 8 Length of query: 379 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 292 Effective length of database: 1,207,925 Effective search space: 352714100 Effective search space used: 352714100 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)