BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0232.p (586 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hy... 907 0.0 gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hy... 593 e-170 gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate ... 91 3e-19 >gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 589 Score = 907 bits (2343), Expect = 0.0, Method: Compositional matrix adjust. Identities = 447/558 (80%), Positives = 478/558 (85%), Gaps = 7/558 (1%) Query: 1 MNPTPAIDYQQQRFMYQPCLEQTRPADAALHPVVVIGAGPVGLVTAIDLARQGIRVVLVD 60 MN PAIDYQQQRF + PC EQ P A +HPVV+IGAGPVGL AIDLARQGI VVLVD Sbjct: 1 MNQNPAIDYQQQRFAWTPCAEQQSPGHAPVHPVVIIGAGPVGLACAIDLARQGIAVVLVD 60 Query: 61 DDDTLSTGSRAICFAKRTLDIFDRIGCGQRMVDKGVGWNRGKVFLREEAIYEFDLLPECG 120 DDDTLSTGSRAICFAKRTLDIFDRIGCGQRMVDKGVGWNRGKVFLR++A+YEFDLLPE G Sbjct: 61 DDDTLSTGSRAICFAKRTLDIFDRIGCGQRMVDKGVGWNRGKVFLRDQAVYEFDLLPESG 120 Query: 121 HARPAFINLQQYYVEGYLLEQARQHSLIELRWKSRVSAIAHHADHEALTVETPEGSYLLK 180 HARPAFINLQQYYVEGYLLEQA+Q IE+RWK++VSAIAHH DHEALTV+TP+GSY LK Sbjct: 121 HARPAFINLQQYYVEGYLLEQAQQWPHIEIRWKNKVSAIAHHEDHEALTVDTPQGSYTLK 180 Query: 181 ARYVVAADGSRSPLRKMMGLESVGHTFRDRFLIADVKMAAPFPAERWFWFDPPFHPNQSV 240 ARYVVAADGSRSPLRKMMGLES GHTFRDRFLIADVKM APFPAERWFWFDPPFHP QSV Sbjct: 181 ARYVVAADGSRSPLRKMMGLESQGHTFRDRFLIADVKMDAPFPAERWFWFDPPFHPQQSV 240 Query: 241 LLHRQPDDVWRIDFQLGWDADPVLERTPERVIPRVQALLGKDAQFTLEWVSVYTFSCVRM 300 LLHRQPDDVWRIDFQLGWDADPVLE+TPERVIPRV+ALLG++ F+LEWVSVYTFSCVRM Sbjct: 241 LLHRQPDDVWRIDFQLGWDADPVLEKTPERVIPRVRALLGEEVSFSLEWVSVYTFSCVRM 300 Query: 301 DRFRHGRVLFVGDAAHCVSPFGARGANSGIQDAENLAWKLAFVLQGKAPDSLLDSYASER 360 +RFRHGRVLFVGDAAHCVSPFGARGANSGIQDAENLAWKLA VLQGKAPDSLLDSYASER Sbjct: 301 ERFRHGRVLFVGDAAHCVSPFGARGANSGIQDAENLAWKLAHVLQGKAPDSLLDSYASER 360 Query: 361 EFAADENLLNSTRSTDFITPKSAVSRCFRDAVLALAREHAFARRLVNSGRLSLPATLHGS 420 E+AADENLLNSTRSTDFITPKSAVSR FRDAVL LAREHAFARRLVNSGRLSLPATL S Sbjct: 361 EYAADENLLNSTRSTDFITPKSAVSRAFRDAVLGLAREHAFARRLVNSGRLSLPATLDQS 420 Query: 421 PLSSADSA------PFHGAMQAGAPCTDAPVLTDTIPGWLLPHLGDQFVAMIFAPPASLS 474 PL++ D A F G M+ GAPC DAP+L P WLLP LGD+FV MIFA PA L Sbjct: 421 PLNTPDQAAAPEAVAFTGPMRPGAPCCDAPLLQAGQPAWLLPQLGDRFVLMIFAAPAQLP 480 Query: 475 AALLPALVRLTEGSLPLRLLFVSGAQASAEDHTVLSALGDGAVQVQDIEGLVVQRYDGQP 534 AA AL L + LRLLF+S A+ ED VL L A+QVQDI+ L QRYD P Sbjct: 481 AATRQALAALAQPDA-LRLLFISAQAATQEDERVLQTLAGQAIQVQDIDTLAAQRYDAHP 539 Query: 535 HTCYLIRPDQHVCARWRT 552 +CYLIRPDQHV ARWR+ Sbjct: 540 GSCYLIRPDQHVSARWRS 557 >gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 547 Score = 593 bits (1530), Expect = e-170, Method: Compositional matrix adjust. Identities = 305/559 (54%), Positives = 375/559 (67%), Gaps = 29/559 (5%) Query: 9 YQQQRFMYQPCLEQTRPADAALHPVVVIGAGPVGLVTAIDLARQGIRVVLVDDDDTLSTG 68 Y RF Y+ EQ R A + HPVVVIGAGP+GL A++ A +G+ VV++DD+DT+S G Sbjct: 5 YTYPRFAYRQSDEQ-REARPSRHPVVVIGAGPIGLTAALECAARGLPVVVLDDNDTVSIG 63 Query: 69 SRAICFAKRTLDIFDRIGCGQRMVDKGVGWNRGKVFLREEAIYEFDLLPECGHARPAFIN 128 SRA+C+AKR L+I+DR+G RMV+KGV W GKVF E +Y FDLLPE H PA IN Sbjct: 64 SRAVCYAKRPLEIWDRLGVAPRMVEKGVSWKLGKVFFEEREVYRFDLLPEHAHKMPAMIN 123 Query: 129 LQQYYVEGYLLEQARQHSLIELRWKSRVSAIAHHADHEALTVETPEGSYLLKARYVVAAD 188 LQQYY+E YL+E + LI+LRWK ++ ++ H L VETP+G + ++A +V+A D Sbjct: 124 LQQYYLEQYLVEACQAQPLIDLRWKHKMISLHQDDTHATLDVETPDGIFRMEADWVIACD 183 Query: 189 GSRSPLRKMMGLESVGHTFRDRFLIADVKMAAPFPAERWFWFDPPFHPNQSVLLHRQPDD 248 G+ S RKM+G E G F+DRFLIADV M A FPAERWFWFDPPFH QSVLLH+Q D+ Sbjct: 184 GANSDTRKMVGAEFSGQFFQDRFLIADVIMKADFPAERWFWFDPPFHRGQSVLLHKQCDN 243 Query: 249 VWRIDFQLGWDADPVLERTPERVIPRVQALLGKDAQFTLEWVSVYTFSCVRMDRFRHGRV 308 VWRIDFQLGW+ADP E+ PE+VIPR+QA+LGK+ +F LEWVSVY F+C R+D+ R+ RV Sbjct: 244 VWRIDFQLGWEADPEEEKKPEKVIPRIQAMLGKEVEFELEWVSVYQFACRRIDKMRYQRV 303 Query: 309 LFVGDAAHCVSPFGARGANSGIQDAENLAWKLAFVLQGKAPDSLLDSYASEREFAADENL 368 LF GDAAH VSPFGARGAN+G+QD +NL WKL V+ GKAP S +DSY ER +AAD+NL Sbjct: 304 LFAGDAAHQVSPFGARGANTGVQDIDNLVWKLKLVIDGKAPPSFIDSYHEERAWAADDNL 363 Query: 369 LNSTRSTDFITPKSAVSRCFRDAVLALAREHAFARRLVNSGRLSLPATLHGSPLSSADSA 428 STRSTDFITPKS S+ RDAVL LA FAR LVNSGRLS P + SPL++AD+A Sbjct: 364 RQSTRSTDFITPKSRASKALRDAVLGLAETEEFARTLVNSGRLSTPTSYVHSPLNTADAA 423 Query: 429 PFHGAMQAGAPCTDAPVLTDTIPGWLLPHLGDQFVAMIF---APPASLSAALLPALVRLT 485 F G M G C DAPVL + PGWLL LG FV + F AP A + AA + A V Sbjct: 424 LFGGRMAPGTVCADAPVLREGQPGWLLESLGAGFVVLFFGASAPQAVIEAAGISARV--- 480 Query: 486 EGSLPLRLLFVSGAQASAEDHTVLSALGDGAVQVQDIEGLVVQRYDGQPHTCYLIRPDQH 545 + A G GA ++ D EGL RY YL+RPDQH Sbjct: 481 ---------------------VTVGAPGSGA-ELIDREGLAALRYAASEGCVYLVRPDQH 518 Query: 546 VCARWRTVDAAALAQALQR 564 V ARW + +A AL R Sbjct: 519 VAARWNRFHSDDIAAALAR 537 >gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate hydroxylase [Herbaspirillum seropedicae SmR1] Length = 544 Score = 90.5 bits (223), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 93/346 (26%), Positives = 151/346 (43%), Gaps = 22/346 (6%) Query: 25 PADAALHPVVVIGAGPVGLVTAIDLARQGIRVVLVDDDDTLSTGSRAICFAKRTLDIFDR 84 PA V+++GAGPVG+ L GI +L++ + + RA+ L F Sbjct: 11 PAQVMQAQVLIVGAGPVGVTLGNLLGTYGISTLLIERNLDVLDYPRAVGIDDEALRTFQS 70 Query: 85 IGCGQRMVDKGVGWNRGKVFLREEAIYEFDLLP---ECGHARPAFINLQQYYVEGYLLEQ 141 G +V + +++ + + ++ P E G R + Q E L Sbjct: 71 AGMADAIVRDVIQNVPMRMYTADRRCFA-EIQPTTREFGWYRRNLFS--QPLGEATLRLG 127 Query: 142 ARQHSLIELRWKSRV-SAIAHHADHEALTVETPEGSYLLKARYVVAADGSRSPLR-KMMG 199 R+ + LR + + A +A ++ L+A+YVV DG RS +R K++ Sbjct: 128 LRRFEHVSLRSGVELLDLVQDEAGVQASIIDEHGAQATLRAQYVVGCDGGRSTVREKILK 187 Query: 200 LESVGHTFRDRFLIADVK---MAAPFPAERWFWFDPPFHPNQSVLLHRQPDDVWRIDFQL 256 L G T ++++ + + AP+ A P + + P V R +F L Sbjct: 188 LPYEGKTHPAKWVVIECDGDPLDAPYTALH-------CEPQRPYVCLALPYGVRRWEFML 240 Query: 257 --GWDADPVLERTPERVIPRVQALLGKDAQFTLEWVSVYTFSCVRMDRFRHGRVLFVGDA 314 G D + +L PE+V ++ + + VYT + GR+ GDA Sbjct: 241 FPGEDGEQML--APEKVRELLREHVRDVDALNIIRARVYTHNSRVAGSLVCGRICLAGDA 298 Query: 315 AHCVSPFGARGANSGIQDAENLAWKLAFVLQGKAPDSLLDSYASER 360 AH P+ +G N+G++DA NLAWKLA+VLQG+ LL SY ER Sbjct: 299 AHITPPWIGQGLNAGLRDAFNLAWKLAWVLQGRMKPELLASYHEER 344 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,833,498 Number of extensions: 78722 Number of successful extensions: 265 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 261 Number of HSP's successfully gapped: 3 Length of query: 586 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 496 Effective length of database: 1,193,720 Effective search space: 592085120 Effective search space used: 592085120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)