BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0230.p (69 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309876|ref|YP_003773968.1| major facilitator superfamily p... 111 9e-27 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 88 1e-19 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 81 2e-17 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 74 3e-15 gi|300310476|ref|YP_003774568.1| major facilitator superfamily t... 69 7e-14 >gi|300309876|ref|YP_003773968.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 449 Score = 111 bits (278), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 54/61 (88%), Positives = 56/61 (91%) Query: 3 SFSRIMVLWGLASVGMMAVSDATHFYVLRFLLGVFEAGFFPGIVLYLSYWFPARRRVATA 62 +FSRIMVLWGLASVGMMAV+DA HFY LRFLLGVFEAGFFPGIVLYLSYWFPARRR A Sbjct: 98 TFSRIMVLWGLASVGMMAVTDAAHFYTLRFLLGVFEAGFFPGIVLYLSYWFPARRRAAVM 157 Query: 63 A 63 A Sbjct: 158 A 158 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 87.8 bits (216), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 38/56 (67%), Positives = 48/56 (85%) Query: 3 SFSRIMVLWGLASVGMMAVSDATHFYVLRFLLGVFEAGFFPGIVLYLSYWFPARRR 58 + +RI +LWGLAS+ M V+ T FY+LRFLLG+FEAGFFPG+VLYL+YWFPA+RR Sbjct: 98 TLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGVVLYLTYWFPAQRR 153 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 80.9 bits (198), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 35/59 (59%), Positives = 45/59 (76%) Query: 5 SRIMVLWGLASVGMMAVSDATHFYVLRFLLGVFEAGFFPGIVLYLSYWFPARRRVATAA 63 +RIM+ WG+ S M V +AT FY+LRFLLG+ EAGF+PGI+LYL+YWFP+ RR A Sbjct: 97 ARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAGFYPGIILYLTYWFPSHRRAKVIA 155 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 73.6 bits (179), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 32/54 (59%), Positives = 42/54 (77%) Query: 5 SRIMVLWGLASVGMMAVSDATHFYVLRFLLGVFEAGFFPGIVLYLSYWFPARRR 58 +RIM+ WG+ S M V+ FY++RFLLGV EAGFFPG++LYL+YW+PA RR Sbjct: 96 ARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGVILYLTYWYPAARR 149 >gi|300310476|ref|YP_003774568.1| major facilitator superfamily transporter protein [Herbaspirillum seropedicae SmR1] Length = 449 Score = 68.9 bits (167), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 31/54 (57%), Positives = 37/54 (68%) Query: 5 SRIMVLWGLASVGMMAVSDATHFYVLRFLLGVFEAGFFPGIVLYLSYWFPARRR 58 +RIM WGLA+ M V+ T FYVLRFLLG EAGFFPG++ Y + W PA R Sbjct: 105 TRIMATWGLAATAMAFVNSETTFYVLRFLLGAAEAGFFPGVIYYFTQWLPASER 158 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.336 0.144 0.462 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 161,477 Number of extensions: 4723 Number of successful extensions: 46 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 45 Number of HSP's successfully gapped: 9 Length of query: 69 Length of database: 1,619,870 Length adjustment: 41 Effective length of query: 28 Effective length of database: 1,425,735 Effective search space: 39920580 Effective search space used: 39920580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.7 bits) S2: 123 (52.0 bits)