BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0229.p (297 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309876|ref|YP_003773968.1| major facilitator superfamily p... 484 e-138 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 187 1e-48 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 165 3e-42 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 151 6e-38 gi|300313342|ref|YP_003777434.1| major facilitator superfamily p... 150 1e-37 >gi|300309876|ref|YP_003773968.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 449 Score = 484 bits (1247), Expect = e-138, Method: Compositional matrix adjust. Identities = 267/287 (93%), Positives = 277/287 (96%) Query: 1 VMAIFFAGVAVAGVLGGLVSGWIMRDMAGVMGLYGWQWMFAIEGAPAVVLGVLVIFLLAD 60 VMAIFFAGVAVAGVLGGLVSGWIMRDMAGVMGLYGWQWMFAIEGAPAVVLG+L IFLLAD Sbjct: 156 VMAIFFAGVAVAGVLGGLVSGWIMRDMAGVMGLYGWQWMFAIEGAPAVVLGLLAIFLLAD 215 Query: 61 GPAQATWLTPEERSFLQNRQSQEALRTHHVSAHSLTALKQALSHRPTYLFAFIYFALTCG 120 GPAQA WL+PEERSFLQ R QEALRTHHVS HSL AL+QALSHR TYLFAFIYFALTCG Sbjct: 216 GPAQAKWLSPEERSFLQARHQQEALRTHHVSGHSLAALRQALSHRTTYLFAFIYFALTCG 275 Query: 121 SLTLNFWMPLMIRDFGIKDVVQVSLYTVIPNAIGAIGLILIARRSDRRNERHGHFLVCTT 180 SLTLNFWMPLMIRDFGIKDVV+VSLY+VIPNAIGA+GLILIARRSDRRNERHGHF VCTT Sbjct: 276 SLTLNFWMPLMIRDFGIKDVVEVSLYSVIPNAIGAVGLILIARRSDRRNERHGHFAVCTT 335 Query: 181 GGALALAALTLHPSSFAVSLGLLSVAAVLIFAALPIFWSLPPGLLPSQTIAPGIAFISSV 240 GGALALAALTLHPSSFAVSLGLLSVAAVLIFAALPIFWSLPPGLLP+QTIAPGIAFISSV Sbjct: 336 GGALALAALTLHPSSFAVSLGLLSVAAVLIFAALPIFWSLPPGLLPAQTIAPGIAFISSV 395 Query: 241 GITSGIVSPWLIGLVKTRTGNIDIALYILAGLLLLSGLTMWTMIPRK 287 GITSGIVSPWLIGLVKTRTGN+DIALYILAGLLLLSGL MW++IPRK Sbjct: 396 GITSGIVSPWLIGLVKTRTGNMDIALYILAGLLLLSGLAMWSLIPRK 442 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 187 bits (474), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 108/287 (37%), Positives = 163/287 (56%), Gaps = 7/287 (2%) Query: 1 VMAIFFAGVAVAGVLGGLVSGWIMRDMAGVMGLYGWQWMFAIEGAPAVVLGVLVIFLLAD 60 + A+F G+A++GV+GG +SGWIM M GV G GWQWMF +E P+++LGV+VI + D Sbjct: 152 ITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAIPSLILGVMVILYMKD 211 Query: 61 GPAQATWLTPEERSFLQNRQSQEALRTHHVSAHSLTALKQALSHRPTYLFAFIYFALTCG 120 A WL+ EE+ L+ S E + H+S L ++ +L A IYF G Sbjct: 212 RIRDADWLSEEEKRLLEANISHEESQKEHLS------LGAMFANGRVWLGALIYFCFVMG 265 Query: 121 SLTLNFWMPLMIRDFGIKDVVQVSLYTVIPNAIGAIGLILIARRSDRRNERHGHFLVCTT 180 ++FW+P +I+ G+ D + V L T IP A A ++LI R +D R ER H + Sbjct: 266 LYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARRERRWHVAIPAF 325 Query: 181 GGALALAALTLHPSSFAVSLGLLSVAAVLIFAALPIFWSLPPGLLPSQTIAPGIAFISSV 240 G + L T + + +++ L++A+V I LP+FWSLP L A GIA I+S+ Sbjct: 326 IGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGTAAAAGIALINSL 385 Query: 241 GITSGIVSPWLIGLVKTRTGNIDIALYILAGLLLLSGLTMWTMIPRK 287 G +G VSP+L+G +K T + + +Y+LA L++ GL T+ P K Sbjct: 386 GNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVIGGLLTLTL-PAK 431 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 165 bits (418), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 102/278 (36%), Positives = 147/278 (52%), Gaps = 5/278 (1%) Query: 1 VMAIFFAGVAVAGVLGGLVSGWIMRDMAGVMGLYGWQWMFAIEGAPAVVLGVLVIFLLAD 60 V+A+F +G+ VAG+LG +SGWIM GL GWQWMF IE PAV++GV + L + Sbjct: 153 VIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLIEAIPAVLIGVATVLYLDN 212 Query: 61 GPAQATWLTPEERSFLQNRQSQEALRTHHVSAHSLTALKQALSHRPTYLFAFIYFALTCG 120 A WL EE++ LQ +A S H A+ + +L IYF+ G Sbjct: 213 DVKSAKWLNDEEKASLQADIDGDAKGKE--SKHGFGAI---VKDARVWLMCLIYFSFVMG 267 Query: 121 SLTLNFWMPLMIRDFGIKDVVQVSLYTVIPNAIGAIGLILIARRSDRRNERHGHFLVCTT 180 L WMP +++ G+K +++ L + IP I + LI R +DR ER H ++ Sbjct: 268 QYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRSADRMRERRWHLVIPAL 327 Query: 181 GGALALAALTLHPSSFAVSLGLLSVAAVLIFAALPIFWSLPPGLLPSQTIAPGIAFISSV 240 G + L + AVS+ LS+AA + P+FWSLP L A GIA I+SV Sbjct: 328 MGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTAFLSGAAAAVGIAAINSV 387 Query: 241 GITSGIVSPWLIGLVKTRTGNIDIALYILAGLLLLSGL 278 G +G VSP+LIG +K T N +Y+LA +L++ + Sbjct: 388 GNLAGFVSPYLIGYLKDLTHNNATGMYMLAVMLVVGSI 425 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 151 bits (382), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 95/287 (33%), Positives = 157/287 (54%), Gaps = 7/287 (2%) Query: 1 VMAIFFAGVAVAGVLGGLVSGWIMRDMAGVMGLYGWQWMFAIEGAPAVVLGVLVIFLLAD 60 V +F A+AG +GG ++G IM M GV L WQW+F +EG P+++ G V+ L + Sbjct: 156 VNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGIPSILAGFAVLAWLPE 215 Query: 61 GPAQATWLTPEERSFL-QNRQSQEALRTHHVSAHSLTALKQALSHRPTYLFAFIYFALTC 119 PA A WL+ E++ + Q ++ + H S K A ++ ++ A +YF + Sbjct: 216 RPANAKWLSEAEKNAVTQVLAAENSAPGKHFS------FKDACTNYRVWICAAVYFCVVS 269 Query: 120 GSLTLNFWMPLMIRDFGIKDVVQVSLYTVIPNAIGAIGLILIARRSDRRNERHGHFLVCT 179 G+ T+ FW P +I++ G+K +Q+ L + IP G + +I SD+ ER H + T Sbjct: 270 GNATIAFWAPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHSDKTGERRLHSAIAT 329 Query: 180 TGGALALAALTLHPSSFAVSLGLLSVAAVLIFAALPIFWSLPPGLLPSQTIAPGIAFISS 239 + L A + + ++L L++AA+ I AA P+FWS+P L A GIA I+S Sbjct: 330 LIACIGLVATGMTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFLAGTAAAGGIALINS 389 Query: 240 VGITSGIVSPWLIGLVKTRTGNIDIALYILAGLLLLSGLTMWTMIPR 286 +G +G V+P++IG +KT TG++ LY +A L L++ + I + Sbjct: 390 IGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALELVASFLVVVFIRK 436 >gi|300313342|ref|YP_003777434.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 150 bits (378), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 94/278 (33%), Positives = 149/278 (53%), Gaps = 6/278 (2%) Query: 1 VMAIFFAGVAVAGVLGGLVSGWIMRDMAGVMGLYGWQWMFAIEGAPAVVLGVLVIFLLAD 60 VMA+ G + +LGG +SG +M+ M L GWQWMF IE PA +LG++++F+L++ Sbjct: 156 VMAVVLLGNPFSYILGGPISGALMQFMGSGDSLAGWQWMFVIEALPACLLGIVILFVLSN 215 Query: 61 GPAQATWLTPEERSFLQNRQSQEALRTHHVSAHSLTALKQALSHRPTYLFAFIYFALTCG 120 A A WL PEE++ L R + E +V SL AL + A +Y + G Sbjct: 216 DIASAKWLAPEEKAELARRLAAENKEKVNVPLKSLYALPI------LWFLALVYLLMMIG 269 Query: 121 SLTLNFWMPLMIRDFGIKDVVQVSLYTVIPNAIGAIGLILIARRSDRRNERHGHFLVCTT 180 S +NFW P +IRD G+K V+ + + + IP +GA ++ I R +++ + + + + Sbjct: 270 SYGINFWAPTIIRDAGVKSVLDIGMISAIPYILGATAMVFITRHAEKAGKHRSYSVAVSM 329 Query: 181 GGALALAALTLHPSSFAVSLGLLSVAAVLIFAALPIFWSLPPGLLPSQTIAPGIAFISSV 240 LAL TL P + ++L +++A ++ +FW P T A GIA I+SV Sbjct: 330 VAGLALVLCTLFPHNIYITLAGIAIAVGGSLTSIALFWGFPGAYFTGATAAAGIAIINSV 389 Query: 241 GITSGIVSPWLIGLVKTRTGNIDIALYILAGLLLLSGL 278 G G V P+L+G + T G+ L +L G L L+G+ Sbjct: 390 GNLGGFVGPYLLGALTTAFGSPRYGLMMLGGALFLAGV 427 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.141 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 741,858 Number of extensions: 25160 Number of successful extensions: 162 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 152 Number of HSP's successfully gapped: 9 Length of query: 297 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 212 Effective length of database: 1,217,395 Effective search space: 258087740 Effective search space used: 258087740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 130 (54.7 bits)