BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0227.p (500 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309874|ref|YP_003773966.1| nitrate/sulfonate/bicarbonate A... 871 0.0 gi|300309717|ref|YP_003773809.1| nitrate/sulfonate/bicarbonate A... 176 3e-45 gi|300312685|ref|YP_003776777.1| nitrate/sulfonate/bicarbonate A... 170 2e-43 gi|300309802|ref|YP_003773894.1| nitrate/sulfonate/bicarbonate A... 163 3e-41 gi|300310542|ref|YP_003774634.1| nitrate transporter ATPase [Her... 159 3e-40 >gi|300309874|ref|YP_003773966.1| nitrate/sulfonate/bicarbonate ABC transporter ATPase/permease [Herbaspirillum seropedicae SmR1] Length = 447 Score = 871 bits (2250), Expect = 0.0, Method: Compositional matrix adjust. Identities = 427/436 (97%), Positives = 432/436 (99%) Query: 1 MTMTSPQQRPAVAELCAVSKSYNTAGGHELKVLDNIDLAVHEGELLALLGQSGSGKSTIL 60 MTMTSPQQRPAVAELCAVSKSYNTAGGHELKVLDNIDLAVHEGELLALLGQSGSGKSTIL Sbjct: 1 MTMTSPQQRPAVAELCAVSKSYNTAGGHELKVLDNIDLAVHEGELLALLGQSGSGKSTIL 60 Query: 61 RCLTGLVEPSSGHTMAAGKQLNGVNRDASVVFQTFALYPWLTVEQNVAVGLMSHNPTREE 120 RCLTGLVEPSSGHTMAAGK L GVNRDASVVFQTFALYPWLTVEQNVAVGLMSHNPTREE Sbjct: 61 RCLTGLVEPSSGHTMAAGKPLYGVNRDASVVFQTFALYPWLTVEQNVAVGLMSHNPTREE 120 Query: 121 RDAAVDRAIELIGLGNYHSAYPRELSGGMRQRVGIARALVSKPKLLCLDEAFSALDVLTA 180 RDAAVDRAIELIGLGNYHSAYPRELSGGMRQRVGIARALVSKPKLLCLDEAFSALDVLTA Sbjct: 121 RDAAVDRAIELIGLGNYHSAYPRELSGGMRQRVGIARALVSKPKLLCLDEAFSALDVLTA 180 Query: 181 ENLRQELISLWRAPDSGLTSIFMVTHNIEEAVEMATRIVVVFPRPGRLGLVLENPLPYPR 240 ENLRQELISLWRAPDSGLTSIFMVTHNIEEAVEMATRIVVVFPRPGRLGLVL+NPLPYPR Sbjct: 181 ENLRQELISLWRAPDSGLTSIFMVTHNIEEAVEMATRIVVVFPRPGRLGLVLDNPLPYPR 240 Query: 241 DSKSPEFQRLVNIIHEAITTLSLPDLPPEVHVPGEPISRGRKRMESIPLIPVGQILGLMS 300 D+KSPEFQRLVNIIHEAITTL+LPDLPPEVHVPGEPISRGRKRMESIPLIPVGQILGLMS Sbjct: 241 DTKSPEFQRLVNIIHEAITTLTLPDLPPEVHVPGEPISRGRKRMESIPLIPVGQILGLMS 300 Query: 301 LLHDEPELNNIYDISDEIGKEFGETIGTVKAAEILELVDTPKQEVYLTDLGRRFVAADRK 360 LLHDEPELNNIYDISDEIGKEFGETI TVKAAEILELVDTPKQEVYLTDLGRRFVAADR Sbjct: 301 LLHDEPELNNIYDISDEIGKEFGETIATVKAAEILELVDTPKQEVYLTDLGRRFVAADRL 360 Query: 361 GQRAIFAEQVYKLRLFHIILAVLGEAEEIEAERVLKDIGSALPYDNPEKTFETMIAWGRY 420 GQRAIFAEQVYKLR+FHIILA+LGEAEEIEAERVLKDIGSALPYDNPEKTFETMIAWGRY Sbjct: 361 GQRAIFAEQVYKLRIFHIILALLGEAEEIEAERVLKDIGSALPYDNPEKTFETMIAWGRY 420 Query: 421 AGLMDYNAKTGMVFVP 436 AGLMDYNAKTGMVFVP Sbjct: 421 AGLMDYNAKTGMVFVP 436 >gi|300309717|ref|YP_003773809.1| nitrate/sulfonate/bicarbonate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 269 Score = 176 bits (447), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 101/249 (40%), Positives = 144/249 (57%), Gaps = 9/249 (3%) Query: 2 TMTSPQQRPAVAELCAVSKSYNTAGGHELKVLDNIDLAVHEGELLALLGQSGSGKSTILR 61 T SP+ R + V K + T E+ L +I+L + +G+ + LLG SG GKST+L Sbjct: 4 TNASPRIR-----IQGVHKVFKT-DDREVVALKDINLDIPDGQFVCLLGPSGCGKSTLLN 57 Query: 62 CLTGLVEPSSGHTMAAGKQLNGVNRDASVVFQTFALYPWLTVEQNVAVGLMSHNPTREER 121 + G PSSG + K ++ D +VFQ +AL+PW+TVEQNVA GL + E Sbjct: 58 AIAGFALPSSGQILTDDKVVSEPGPDRGMVFQEYALFPWMTVEQNVAFGLEIKGTPKAEI 117 Query: 122 DAAVDRAIELIGLGNYHSAYPRELSGGMRQRVGIARALVSKPKLLCLDEAFSALDVLTAE 181 V + ++ +GL ++ + +P++LSGGMRQRV IAR L ++ +DE F ALD LT Sbjct: 118 HQRVTQLLDKLGLIDFRTRFPKDLSGGMRQRVAIARVLALDSPIMLMDEPFGALDALTRR 177 Query: 182 NLRQELISLWRAPDSGLTSIFMVTHNIEEAVEMATRIVVVFPRPGRLGLVLENPLPYPRD 241 NL+ EL+ +W D +I VTH+IEEA+ +A RIVV+ RPG + + LP RD Sbjct: 178 NLQDELLRIW---DEFRKTIVFVTHSIEEAIYLADRIVVMTYRPGTVKRDMLVNLPRLRD 234 Query: 242 SKSPEFQRL 250 PEF L Sbjct: 235 PADPEFNAL 243 >gi|300312685|ref|YP_003776777.1| nitrate/sulfonate/bicarbonate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 275 Score = 170 bits (431), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 99/267 (37%), Positives = 152/267 (56%), Gaps = 10/267 (3%) Query: 7 QQRPAVAELCAVSKSYNTAGGHELKVLDNIDLAVHEGELLALLGQSGSGKSTILRCLTGL 66 Q+ PA+ EL VS + TA G L + ++V GE +A++G +G GKST L +TGL Sbjct: 17 QETPAI-ELRNVSCRFITADGRATVALRDFSMSVARGEFVAVVGPTGCGKSTTLSLITGL 75 Query: 67 VEPSSGHTMAAGKQLNGVNRDASVVFQTFALYPWLTVEQNVAVGLMSHNPTREERDAAVD 126 ++P+ G G+ +NG++ VFQ A++PW +V +NVA G + + + A D Sbjct: 76 LKPTLGEVRVMGQPVNGIDPRIGFVFQNDAVFPWRSVRENVAAGPLFRGQPKAQAHALAD 135 Query: 127 RAIELIGLGNYHSAYPRELSGGMRQRVGIARALVSKPKLLCLDEAFSALDVLTAENLRQE 186 I +GL + YP +LSGGMR+RV +A+ ++ P++L +DE FSALD+ T ++ E Sbjct: 136 EWIRRVGLDKFGDHYPHQLSGGMRKRVALAQTFINSPEILLMDEPFSALDMQTRTLMQDE 195 Query: 187 LISLWRAPDSGLTSIFMVTHNIEEAVEMATRIVVVFPRPGRLGLVLENPLPYPRDSKSPE 246 L+ LW A SG S+ VTH++EEA+ +A ++ V+ RP L V LP PR + Sbjct: 196 LLRLWSA-HSG--SVVFVTHDLEEAIALADKVYVLTARPATLKSVYPIDLPRPRVMEEVR 252 Query: 247 F-QRLVNIIHEAITTLSLPDLPPEVHV 272 + QR ++I DL EVH+ Sbjct: 253 YEQRFIDISRRI-----WADLREEVHI 274 >gi|300309802|ref|YP_003773894.1| nitrate/sulfonate/bicarbonate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 278 Score = 163 bits (413), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 102/248 (41%), Positives = 140/248 (56%), Gaps = 13/248 (5%) Query: 18 VSKSYNTAGGHELKVLDNIDLAVHEGELLALLGQSGSGKSTILRCLTGLVEPSSGHTMAA 77 V K+Y G H LD + L + GE ++LLG SG GKST+L+ GL EPS GH Sbjct: 25 VEKTYRN-GTH---ALDEVRLDIGRGEFVSLLGPSGCGKSTLLKMFAGLEEPSHGHIRWW 80 Query: 78 GKQLNGV---NRDASVVFQTFALYPWLTVEQNVAVGLMSHNPTREERDAAVDRAIELIGL 134 G L V R ++VFQ L PW TVEQNV + L + R + DA V +++ +GL Sbjct: 81 GGNLATVGTPERTLAMVFQEATLMPWATVEQNVRLPLDLRHVPRAQADARVAASLKRVGL 140 Query: 135 GNYHSAYPRELSGGMRQRVGIARALVSKPKLLCLDEAFSALDVLTAENLRQELISLWRAP 194 ++H+ PRELSGGM+ R IARAL + P LL +DE F ALD T L +L +W Sbjct: 141 ESFHAVLPRELSGGMQMRASIARALATSPNLLLMDEPFGALDEFTRNKLDADLRDIWSRQ 200 Query: 195 DSGLTSIFMVTHNIEEAVEMATRIVVVFPRPGRLGLVLENPLPYPRDSK---SPEFQRLV 251 D LT +F VTH+I EAV +++R++V+ RPGR+ + P PRD S F + Sbjct: 201 D--LTVVF-VTHSIYEAVFLSSRVIVMAARPGRVIADVAIDAPGPRDEAFRMSAPFMQYC 257 Query: 252 NIIHEAIT 259 + + +T Sbjct: 258 KTLSDYLT 265 >gi|300310542|ref|YP_003774634.1| nitrate transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 272 Score = 159 bits (403), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 92/223 (41%), Positives = 137/223 (61%), Gaps = 11/223 (4%) Query: 33 LDNIDLAVHEGELLALLGQSGSGKSTILRCLTGLVEPSSGHTMAAGKQLNGVNRDASVVF 92 L I+L V +GE + L+G SG GKST+L + GL++PS G + A +++ G + +VVF Sbjct: 30 LREINLTVAKGEFVTLIGHSGCGKSTLLNLIAGLLKPSEGVLLCANREIGGPAPERAVVF 89 Query: 93 QTFALYPWLTVEQNVAVGLMSHNPTREERDAAVDR---AIELIGLGNYHSAYPRELSGGM 149 Q +L PWL+ +N+ +G+ E R DR A+ L+GL + P E+SGGM Sbjct: 90 QNHSLLPWLSCYENIYLGVERVFGATENRTQLRDRTRAALALVGLSAAENKRPNEISGGM 149 Query: 150 RQRVGIARALVSKPKLLCLDEAFSALDVLTAENLRQELISLWRAPDSGLTSIFMVTHNIE 209 +QRVGIARAL +PK+L +DE F ALD LT +L+ EL+ + DS ++ MVTH+++ Sbjct: 150 KQRVGIARALAMEPKVLLMDEPFGALDALTRAHLQDELLKIVAKTDS---TVVMVTHDVD 206 Query: 210 EAVEMATRIVVVFPRP-GRLGLVLENPLPYPRD----SKSPEF 247 EAV ++ RIV++ P +G VLE L PRD ++ PE+ Sbjct: 207 EAVLLSDRIVMMTNGPAATIGEVLEVKLARPRDRVVLAQDPEY 249 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,521,274 Number of extensions: 62384 Number of successful extensions: 584 Number of sequences better than 1.0e-08: 124 Number of HSP's gapped: 387 Number of HSP's successfully gapped: 133 Length of query: 500 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 411 Effective length of database: 1,198,455 Effective search space: 492565005 Effective search space used: 492565005 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)