BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0224.p (318 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309872|ref|YP_003773964.1| major facilitator superfamily p... 462 e-131 gi|300314020|ref|YP_003778112.1| major facilitator superfamily p... 184 6e-48 gi|300312966|ref|YP_003777058.1| major facilitator superfamily p... 75 5e-15 gi|300311185|ref|YP_003775277.1| major facilitator superfamily p... 73 3e-14 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 67 2e-12 >gi|300309872|ref|YP_003773964.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 493 Score = 462 bits (1189), Expect = e-131, Method: Compositional matrix adjust. Identities = 270/318 (84%), Positives = 292/318 (91%) Query: 1 LLAALGWEWIFLINVPLGIVAVLLSWSAIPETRKPAQAPFDVAGALWSALAMGAIIMAAD 60 LLA LGWEWIF INVPLG++AV L+W+A+P+TRKPAQA FD+AGALWSALAMGA+IMAAD Sbjct: 176 LLAMLGWEWIFAINVPLGVLAVWLAWNAVPDTRKPAQAAFDLAGALWSALAMGALIMAAD 235 Query: 61 TCARFAEPGQGTHAMLEAAAYALVALLAGAAFVRGQRRVLEPLLPLDIFANARFSLAALT 120 TCARFAEPGQG+ A AAAYA+ ALLAG AFVRGQRR +PLLPLDIFAN RFSLAALT Sbjct: 236 TCARFAEPGQGSGAAFTAAAYAVTALLAGLAFVRGQRRAADPLLPLDIFANPRFSLAALT 295 Query: 121 SLASFVGQGIAFVALPFLFQNGYGYTAFESAALFTPWPLGIVLVAPHAGKLADRHSPALL 180 SLASFVGQGIAFVALPFLFQN YGYTAFESAALFTPWPLGIVLVAPHAG+LADRHSPALL Sbjct: 296 SLASFVGQGIAFVALPFLFQNAYGYTAFESAALFTPWPLGIVLVAPHAGRLADRHSPALL 355 Query: 181 SSLGLALFTVGLALLALLPDHAQAWDIGWRALVCGMGFGFFQSPNNREMLSNVARERSGN 240 SSLGLALF +GL LLALLPD AQ WDIGWRALVCGMGFGFFQSPNNREML NV+RERSGN Sbjct: 356 SSLGLALFALGLVLLALLPDQAQPWDIGWRALVCGMGFGFFQSPNNREMLGNVSRERSGN 415 Query: 241 ASGVLAIMRTFGQCLGAALLGTVLAIYTAGAVAQGEMRMSAAQDGEAIRLALWIAVLATA 300 ASGVLAIMRTFGQCLGAALLG +LA+ TA AVAQGE++M+ QDGEAIR+ALW+A ATA Sbjct: 416 ASGVLAIMRTFGQCLGAALLGVILAVRTAAAVAQGELQMNPVQDGEAIRIALWLAAAATA 475 Query: 301 LATAVSASRLRRKAQSPA 318 LATAVSASRLRRKA+SPA Sbjct: 476 LATAVSASRLRRKAESPA 493 >gi|300314020|ref|YP_003778112.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 475 Score = 184 bits (468), Expect = 6e-48, Method: Compositional matrix adjust. Identities = 108/260 (41%), Positives = 162/260 (62%), Gaps = 8/260 (3%) Query: 1 LLAALGWEWIFLINVPLGIVAVLLSWSAIPETRKPAQAPFDVAGALWSALAMGAIIMAAD 60 +LA W W+F +NVPLG++AV+L+ ++P T A+ FD+ AL +A A G +I+A Sbjct: 178 ILAIAPWPWLFAVNVPLGVLAVVLAVPSLPHTSL-ARHAFDLRSALLNAAAFGLLILAIG 236 Query: 61 TCARFAEPGQGTHAMLEAAAYALVALLAGAAFVRGQRRVLEPLLPLDIFANARFSLAALT 120 A A Q + LE AA A+L G ++ + P+LP+D+F + FSL+A+T Sbjct: 237 EGAHQA---QASVVGLELAA----AILCGLFLLKRELSHPAPMLPVDLFRHPMFSLSAVT 289 Query: 121 SLASFVGQGIAFVALPFLFQNGYGYTAFESAALFTPWPLGIVLVAPHAGKLADRHSPALL 180 ++ SF QG+AFV+LPF F + G + E L TPWP+ + ++AP AG+++DR+ +L Sbjct: 290 AVCSFAAQGLAFVSLPFFFHHDLGRSQVEIGLLMTPWPVAVAIMAPIAGRMSDRYPVGML 349 Query: 181 SSLGLALFTVGLALLALLPDHAQAWDIGWRALVCGMGFGFFQSPNNREMLSNVARERSGN 240 +GLAL GL + +P W+I WR L+CGMGFGFFQSPN + ++++ RSG Sbjct: 350 GGVGLALLCAGLLSMTFMPASPSVWEISWRMLLCGMGFGFFQSPNLKALMTSAPPHRSGG 409 Query: 241 ASGVLAIMRTFGQCLGAALL 260 ASG++A R GQ +GAAL+ Sbjct: 410 ASGIVATARLLGQSVGAALV 429 >gi|300312966|ref|YP_003777058.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 544 Score = 75.5 bits (184), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 80/264 (30%), Positives = 117/264 (44%), Gaps = 12/264 (4%) Query: 5 LGWEWIFLINVPLGIVAVLLSWSAIPETRKPAQAPFDVAGALWSALAMGAIIMAADTCAR 64 LGW W F +N+P ++ + W +PE+R P P D G L S+ A+ + T A Sbjct: 188 LGWRWAFAVNLPACLLLAVAVWRHVPESRNPLPRPLDPLGILSSSSAVFLL-----TWAL 242 Query: 65 FAEPGQGTHAMLEAAAYALVALLAGAAFVRGQRRVLEPLLPLDIFANARFSLAALTSLAS 124 P G + + A L L F+ QRR P+L L +F +A F A A Sbjct: 243 ITGPEHGWTS-VACVARGLGGALLLVLFIAWQRRAAHPMLELSLFRHAGFVAAVAAMFAY 301 Query: 125 FVGQGIAFVALPFLFQNGYGYTAFESAALFTPWPLGIVLVAPHAGKLADRHSPALLSSLG 184 + LP QN G +A+++ P+ L ++L+ +LA S + +LG Sbjct: 302 AASAQVMASLLPLFLQNARGLSAWQTGLAMLPFALAMLLLPQVGSRLARWLSSPQMLALG 361 Query: 185 LALFTVGLALLALLPDHAQAWDIGWRAL---VCGMGFGFFQSPNNREMLSNVARERSGNA 241 L + +G L+ A + W AL V G G G + ++ NV RER+G A Sbjct: 362 LGVTALGNLLMI---QAALSGAAAWTALGMAVLGSGGGLLNGQTQKAIMGNVPRERAGMA 418 Query: 242 SGVLAIMRTFGQCLGAALLGTVLA 265 SG+ R G G A LG VLA Sbjct: 419 SGISTTARFTGVLAGFAGLGAVLA 442 >gi|300311185|ref|YP_003775277.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 532 Score = 73.2 bits (178), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 82/279 (29%), Positives = 133/279 (47%), Gaps = 20/279 (7%) Query: 1 LLAALGWEWIFLINVPLGIVAVLLSWSAIPETRKPAQA---PFDVAGALWSALAMGAIIM 57 L LGW W+F N+P+G++++ W +P R A D GAL +L++G++ + Sbjct: 169 LTQGLGWRWVFYCNLPIGVISLFFVWRFVPHIRHMQHAGKMRLDWPGALLISLSLGSLQL 228 Query: 58 AADTCARFAEPGQG-THAMLEAAAYALVALLAGAAFVRGQRRVLEPLLPLDIFAN-ARFS 115 + P +G T ML A ++ A A + ++R +P+LP ++ + A S Sbjct: 229 LVELL-----PKRGVTSGMLVLLAVSVAAFY---ALWKWEQRAAQPILPFEMLRDRALAS 280 Query: 116 LAALTSLASFVGQGIAFVALPFLFQNGYGYTAFESAALFTPWPLGIVLVAPHAGKLADR- 174 L L+ LA F + A P LFQ G+G + E+ L TP I + + G++ R Sbjct: 281 LFLLSVLAGFAMFSMLMYA-PLLFQGGFGLSPREAGLLITPLVACITVASIVNGRIITRI 339 Query: 175 HSPALLSSLGLALFTVGLALLALLPDHAQAWDIGWRALVCGMGFGFFQSPNNREMLSN-- 232 +P L+ +LG AL L +L D ++ G GFG N + + Sbjct: 340 PNPNLMMNLGFALIAAS-CLGIVLCDRVTGKGFLLAVMLAG-GFGLGLVLPNLTVFAQQA 397 Query: 233 VARERSGNASGVLAIMRTFGQCLGAALLGTVLA-IYTAG 270 ARE G A+ +L +R G LG A+ GT+++ +Y AG Sbjct: 398 AAREHLGIATALLQSLRMIGGMLGTAITGTLVSQMYGAG 436 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 67.0 bits (162), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 49/151 (32%), Positives = 66/151 (43%), Gaps = 14/151 (9%) Query: 7 WEWIFLINVPLGIVAVLLSWSAIPETRKPAQAPFDVAGALWSALAMGAIIMAADTCARFA 66 W WIF IN+P+ I+ + L+ IP+ + AP D+ G L SAL ++M T R Sbjct: 140 WRWIFFINIPISILGLYLATRFIPDLKAEQVAPLDLKGFLLSALGCSLLMMGLATAGRHL 199 Query: 67 EPGQGTHAMLEAAAYALVALLAG----AAFVRGQRRVLEPLLPLDIFANARFSLAALTSL 122 G+ AL +LAG AA+V RR PL+ L + Sbjct: 200 VSGE----------VALTCVLAGLVFLAAYVWHARRASAPLIDLALLKIPTLRAGVFGGS 249 Query: 123 ASFVGQGIAFVALPFLFQNGYGYTAFESAAL 153 VG G LP + Q G+GYT F S L Sbjct: 250 FYRVGIGAIPFLLPLMLQLGFGYTPFHSGML 280 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.136 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 898,220 Number of extensions: 34508 Number of successful extensions: 254 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 245 Number of HSP's successfully gapped: 11 Length of query: 318 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 233 Effective length of database: 1,217,395 Effective search space: 283653035 Effective search space used: 283653035 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)