BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0192.p (522 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309852|ref|YP_003773944.1| SAM-dependent methyltransferase... 895 0.0 gi|300310113|ref|YP_003774205.1| SAM-dependent methyltransferase... 197 1e-51 >gi|300309852|ref|YP_003773944.1| SAM-dependent methyltransferase [Herbaspirillum seropedicae SmR1] Length = 525 Score = 895 bits (2314), Expect = 0.0, Method: Compositional matrix adjust. Identities = 450/524 (85%), Positives = 485/524 (92%), Gaps = 3/524 (0%) Query: 1 LTNWNDGYVVDIAYTYGYYPGMNPARLPLAFAFHGLQAPAIQTACELGFGQGLSTNFHAA 60 + NWNDGYVVDIAYTYGYYPGMNPARLPLAFAFHGLQAPA+QTACELGFGQGLS+N HAA Sbjct: 1 MNNWNDGYVVDIAYTYGYYPGMNPARLPLAFAFHGLQAPAVQTACELGFGQGLSSNIHAA 60 Query: 61 ASTTRWWGNDFNPLQAAFAQELAAASGSGAQLEDSSFSHFCRRDDLPDFDFIALHGIWSW 120 ASTTRWWGNDFNP QAAFAQELAAASGSGAQLEDSSFS FCRRDDLPDFDFIALHGIWSW Sbjct: 61 ASTTRWWGNDFNPRQAAFAQELAAASGSGAQLEDSSFSRFCRRDDLPDFDFIALHGIWSW 120 Query: 121 VSEDNREIILDFIDRKLKPGGVLYLSYNTQPGWSAFMPLRDLLVQHTQLAGNEERGLPTA 180 VS++NR+II DFIDRKLKPGGVLY+SYNTQPGWSAFMPLR+LLVQHTQ +GNEE+GLPTA Sbjct: 121 VSQENRDIIFDFIDRKLKPGGVLYISYNTQPGWSAFMPLRELLVQHTQQSGNEEQGLPTA 180 Query: 181 ARIEAALAFATRLFETDPVYAQTNPFMRDRLKMLQQQSRSYLAHEYFNRDWHAEPFAEIH 240 ARI+AALAFA RLFE DPVYAQ NPFMRDRLK+LQQQSRSYLAHEYFNRDWH E FA++H Sbjct: 181 ARIDAALAFAARLFEADPVYAQANPFMRDRLKVLQQQSRSYLAHEYFNRDWHVESFADMH 240 Query: 241 RRFSQAGLQFACPAHYIDHLDMANLTPAQRQCLAEIDDPVLYQSTRDFMVNQTFRRDYWV 300 RRFS AGL+FACPAHY+DHLDMANLTPAQRQ L EI DPVL QS RDFMVNQ FRRDYWV Sbjct: 241 RRFSGAGLEFACPAHYVDHLDMANLTPAQRQQLYEITDPVLQQSVRDFMVNQQFRRDYWV 300 Query: 301 RGGQVLDERQRVQALALQSVVLLTEPDKVPLTIQNVAAEITLNRLIYEPILQALSDYQPH 360 RG Q LD+RQRVQALALQSV+LLTEPDKVPLTIQNVA+EITLNRLIYEPILQALSDYQPH Sbjct: 301 RGAQALDDRQRVQALALQSVILLTEPDKVPLTIQNVASEITLNRLIYEPILQALSDYQPH 360 Query: 361 TLVDLAASLAHRNLNLSQVIDSALMLIGTGHAAPLPPELDAATQAQIARRTADLNAYLLQ 420 TLVDLAASLAHRNLNL+QV+DS LMLIGTGHAAPLP E DAAT+AQI RRT DLNA+LLQ Sbjct: 361 TLVDLAASLAHRNLNLAQVLDSVLMLIGTGHAAPLPAEPDAATRAQIERRTGDLNAWLLQ 420 Query: 421 RAALVLP---ESQEGDIAHLVSPLTGGGVKVDRVEQLFLLAREVCGDDVEAWPAQAWQHI 477 RAAL P + +EG+I HL SP+ GGGV+VDRVEQLFLLAR+ CGDDV+AWPAQ W++I Sbjct: 421 RAALASPAGEQGEEGEITHLSSPVIGGGVRVDRVEQLFLLARQSCGDDVDAWPAQVWRYI 480 Query: 478 AAQGKRLVRDGVRLEAEDENLAELATQARLFARTRLPLLRALQV 521 AAQGKRL+R+GVRLE E ENLAELATQARLFARTRLPLL+AL V Sbjct: 481 AAQGKRLLREGVRLEGEAENLAELATQARLFARTRLPLLKALGV 524 >gi|300310113|ref|YP_003774205.1| SAM-dependent methyltransferase [Herbaspirillum seropedicae SmR1] Length = 523 Score = 197 bits (502), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 163/533 (30%), Positives = 247/533 (46%), Gaps = 32/533 (6%) Query: 3 NWNDGYVVDIAYTYGYYPGMNPARLPLAFAFHGLQAPAIQ---TACELGFGQGLSTNFHA 59 W+ GYV I Y G++P +P L A G++ + T ELG GQGL++ A Sbjct: 2 QWSSGYVHGIDYPVGFFPEQSPVHLSFACLAQGVEPVPLDREFTYMELGCGQGLTSTVLA 61 Query: 60 AAS-TTRWWGNDFNPLQAAFAQELAAASG-SGAQLEDSSFSHFCRRD-DLPDFDFIALHG 116 AA+ R++ DF P Q AQ++A+A+G + + SF R + DLP FDF+ ++G Sbjct: 62 AANPQGRFYAVDFLPTQVRTAQDMASAAGIDNLTVLECSFEQMARGEIDLPAFDFVTMYG 121 Query: 117 IWSWVSEDNREIILDFIDRKLKPGGVLYLSYNTQPGWSAFMPLRDLLVQHTQLAGNEERG 176 ++SWV NR ILDF+ R LKPGG++YL+YN PGWS+ + L+ +L + ++ Sbjct: 122 VYSWVDRTNRGHILDFLRRYLKPGGIVYLNYNALPGWSSALSLQRML---REFGRHDMCD 178 Query: 177 LPTAARIEAALAFATRLFETDPVYAQTNPFMRDRLKMLQQQSRSYLAHEYFNRDWHAEPF 236 AL A A +R RL+ L+ YLAHEY N+ W A Sbjct: 179 RQAQLHQGGALVRALSQAGAAAFGAAEATGLRRRLRALESDEPGYLAHEYLNQGWDALFS 238 Query: 237 AEIHRRFSQAGLQFACPAHYIDHLDMANLTPAQRQCLAEIDDPVLYQSTRDFMVNQTFRR 296 ++ + +QA L + A L+ AQ+ LA+ + + + D + N +FR Sbjct: 239 IDVIQDLAQAKLDYVGSAELAYGFPELYLSHAQQSLLAQQPNAGMRELVWDQICNSSFRE 298 Query: 297 DYWVRGGQVLDERQRVQALALQSVVLLTEPDKV----PLTIQNVAAEITLNRLIYEPILQ 352 D +VRG + + QR L + L P ++ PL I NV A+ Y+P++ Sbjct: 299 DIYVRGARRMSPHQRDAWLERCGLALTEAPSRITLELPLAIGNVRADAH----TYQPVMD 354 Query: 353 ALSDYQPHTLVDLAA--SLAHRNLNLSQVIDSALMLIGTGHAAPLPPELDAATQAQIARR 410 AL +PH+ +LAA +L + L ++ A++L + AAP + AQ + Sbjct: 355 ALCQ-RPHSFAELAALPALRKQGRGLREIASMAILLSASRQAAP----FFLSAAAQPRQA 409 Query: 411 TADLNAYLLQRAALVLPESQEGDIAH-LVSPLTGGGVKVDRVEQLFLLAREVCGDDVEAW 469 LN L+ S E D L SPL G G+ ++++ E W Sbjct: 410 AQRLN-------RLIAGWSAESDCFQALASPLLGNGLPSGLLQRVAFDQLSQAQSQPEQW 462 Query: 470 PAQAWQHIAAQGKRLVRDGVRLEAEDENLAELATQARLFARTRLPLLRALQVA 522 Q I QG L R G L +E AEL A+ RLP+ L +A Sbjct: 463 VLPMAQAIHRQGTHLQRGGAPLSTREEIHAELLALAQAICTQRLPVWANLNMA 515 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,606,048 Number of extensions: 65341 Number of successful extensions: 199 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 194 Number of HSP's successfully gapped: 2 Length of query: 522 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 432 Effective length of database: 1,193,720 Effective search space: 515687040 Effective search space used: 515687040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)