BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0184.p (249 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 443 e-126 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 407 e-115 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 153 1e-38 gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herba... 144 5e-36 gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 138 4e-34 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 443 bits (1140), Expect = e-126, Method: Compositional matrix adjust. Identities = 227/249 (91%), Positives = 240/249 (96%) Query: 1 MLLKNKVAVITGGAGLNGLGFSTARLMAAQGARVVILDLERAEPSTAAARLGEGHLGLVA 60 MLLK+KVA+ITGGAG NGLGFSTARLMAAQGAR+VILDLERAEP+ AAARLG HLGLVA Sbjct: 1 MLLKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAARLGAQHLGLVA 60 Query: 61 DVTDKASCEAAAQAILQQFGRIDILVNNAGITQPAKFLDISGKDYDRILDVSLRGTLYMS 120 DVTDKA+CEAAA A+L+QFGRIDILVNNAG+TQPAK L+I+GKDYDRILDVSLRGTLYMS Sbjct: 61 DVTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGTLYMS 120 Query: 121 QAVLPAMRAQQGGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGADGIRV 180 QAVLPAM+AQQ GSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFG GIRV Sbjct: 121 QAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQGIRV 180 Query: 181 NCVTPGLISTDINKDILSAEKKAEIAQTIPLGRLGGPDDVGGVCVFLASDLSQYCTGITL 240 NC+TPGLISTDINKDILSAEKKAEIAQTIPLGRLGGPDDVGGVCVFLASDLSQYCTG+TL Sbjct: 181 NCITPGLISTDINKDILSAEKKAEIAQTIPLGRLGGPDDVGGVCVFLASDLSQYCTGVTL 240 Query: 241 DVNGGMLIH 249 DVNGGMLIH Sbjct: 241 DVNGGMLIH 249 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 407 bits (1045), Expect = e-115, Method: Compositional matrix adjust. Identities = 209/249 (83%), Positives = 226/249 (90%) Query: 1 MLLKNKVAVITGGAGLNGLGFSTARLMAAQGARVVILDLERAEPSTAAARLGEGHLGLVA 60 MLLK+KVAVITGGAG+NGLG++TARLMA QGARVV+LDL +A+P+ AAAR+G HLGLVA Sbjct: 1 MLLKDKVAVITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAARIGSQHLGLVA 60 Query: 61 DVTDKASCEAAAQAILQQFGRIDILVNNAGITQPAKFLDISGKDYDRILDVSLRGTLYMS 120 DVTDKASCE AA AILQQFGRIDILVNNAGITQP K L+I+G DYDRILDVSLRGTLYMS Sbjct: 61 DVTDKASCETAAAAILQQFGRIDILVNNAGITQPVKTLEITGADYDRILDVSLRGTLYMS 120 Query: 121 QAVLPAMRAQQGGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGADGIRV 180 QAVLPAMRAQQ GSI+CISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFG +GIRV Sbjct: 121 QAVLPAMRAQQNGSIICISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPEGIRV 180 Query: 181 NCVTPGLISTDINKDILSAEKKAEIAQTIPLGRLGGPDDVGGVCVFLASDLSQYCTGITL 240 N +TPGLI TDI K L+ EKKAEIA+TIPL RLG DD+GG CVFL SDLSQYCTGITL Sbjct: 181 NSITPGLIGTDIIKGKLTEEKKAEIAETIPLARLGRADDIGGACVFLGSDLSQYCTGITL 240 Query: 241 DVNGGMLIH 249 DVNGGMLIH Sbjct: 241 DVNGGMLIH 249 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 153 bits (386), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 100/252 (39%), Positives = 148/252 (58%), Gaps = 13/252 (5%) Query: 3 LKNKVAVITGGAGLNGLGFSTARLMAAQGARVVILDLERAEPSTAA-----ARLG-EGHL 56 L+ +VA++TG + G+G + A +A QGA+V+ +E AA A LG E Sbjct: 6 LQGQVALVTGAS--RGIGRAIATELARQGAKVI--GTATSESGAAAITEYLAALGPEAGR 61 Query: 57 GLVADVTDKASCEAAAQAILQQFGRIDILVNNAGITQPAKFLDISGKDYDRILDVSLRGT 116 G V +V D + A + + + FG + ILVNNAGITQ + + +++D ++ +L Sbjct: 62 GAVLNVNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAV 121 Query: 117 LYMSQAVLPAMRAQQGGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGAD 176 +S+AVL M + G I+ I+SV + G G +Y+AAKAGV G++RA+ARE G+ Sbjct: 122 GRLSRAVLRGMMKARTGRIINITSVVGEAGNP--GQMNYAAAKAGVAGMSRALAREIGSR 179 Query: 177 GIRVNCVTPGLISTDINKDILSAEKKAEIAQTIPLGRLGGPDDVGGVCVFLASDLSQYCT 236 I VNCV PG I TD+ + L+ ++ A I Q IPLGRLG +++ FLAS + Y T Sbjct: 180 NITVNCVAPGFIDTDMTR-ALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYIT 238 Query: 237 GITLDVNGGMLI 248 G TL+VNGGM + Sbjct: 239 GTTLNVNGGMYM 250 >gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 253 Score = 144 bits (364), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 79/191 (41%), Positives = 121/191 (63%), Gaps = 3/191 (1%) Query: 60 ADVTDKASCEAAAQAILQQFGRIDILVNNAG-ITQPAKFLDISGKDYDRILDVSLRGTLY 118 ADV D ++ AA + Q GRID+L+NNAG + + A ++ + +D I++++ R + Sbjct: 63 ADVRDTSAINAAVAKAVAQMGRIDVLINNAGALVKRAPLETVTDELFDDIININARSVVA 122 Query: 119 MSQAVLPAMRAQQGGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGADGI 178 ++AV+P MRAQ GG+I+ ++SV+A+ GGG G Y+A+K V L R MA+E AD I Sbjct: 123 FTRAVIPVMRAQGGGNIINVTSVAARHGGG-PGALIYAASKGFVSTLTRGMAKELLADKI 181 Query: 179 RVNCVTPGLISTDINKDILSAEKKAEIAQTIPLGRLGGPDDVGGVCVFLASD-LSQYCTG 237 RVN V PG+I T + + E+ +TIP+GR+G PD+ G ++LAS+ +S Y TG Sbjct: 182 RVNAVAPGVIMTPFQERFTTPEQLEGFRKTIPMGRIGEPDECSGAFLYLASEQMSGYVTG 241 Query: 238 ITLDVNGGMLI 248 +DVNGG + Sbjct: 242 QIIDVNGGQYM 252 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 138 bits (348), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 92/247 (37%), Positives = 135/247 (54%), Gaps = 10/247 (4%) Query: 3 LKNKVAVITGGAGLNGLGFSTARLMAAQGARVVILDLERAEPSTAAARLGEGHLGLVADV 62 ++KV V+TGG+ G+G +TA+L AA+GARV + + A +G G +G+ D Sbjct: 7 FEDKVVVVTGGS--EGIGLATAKLFAAEGARVYVSGRRADKLEQAVREIGNGAVGVPGDA 64 Query: 63 TDKASCEAAAQAILQQFGRIDILVNNAGI-TQPAKFLDISG-KDYDRILDVSLRGTLYMS 120 + + GR+D+L NAG + AK L + G +D+D + ++++RG L+ Sbjct: 65 AKLDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFNLNVRGLLFSV 124 Query: 121 QAVLPAMRAQQGGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGADGIRV 180 Q LP M QGG+IV SV+ + G G Y+A+KA V AR+ + GIRV Sbjct: 125 QKALPLM--SQGGAIVLNGSVAGVK--GFPGQSLYNASKAAVRSFARSWTADLKERGIRV 180 Query: 181 NCVTPGLISTDINKDILSAEKKAE--IAQTIPLGRLGGPDDVGGVCVFLASDLSQYCTGI 238 N V PG T + +D L A E + Q +PLGRLG PD++G +FLAS S Y G+ Sbjct: 181 NVVAPGGTETRLMRDYLEARPGMEDVLKQVVPLGRLGEPDEIGRAVLFLASAESSYIAGV 240 Query: 239 TLDVNGG 245 L V+GG Sbjct: 241 ELFVDGG 247 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 811,497 Number of extensions: 35414 Number of successful extensions: 307 Number of sequences better than 1.0e-08: 50 Number of HSP's gapped: 195 Number of HSP's successfully gapped: 50 Length of query: 249 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 166 Effective length of database: 1,226,865 Effective search space: 203659590 Effective search space used: 203659590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)