BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0181.p (667 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum s... 1180 0.0 gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum s... 566 e-162 gi|300309645|ref|YP_003773737.1| transketolase N-terminal subuni... 117 4e-27 gi|300310439|ref|YP_003774531.1| transketolase alpha subunit [He... 106 7e-24 gi|300310438|ref|YP_003774530.1| transketolase, C-terminal subun... 61 3e-10 >gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 1180 bits (3053), Expect = 0.0, Method: Compositional matrix adjust. Identities = 571/659 (86%), Positives = 611/659 (92%) Query: 1 MTPHPLQPLAHAIRFLSIDAIVRATEGHQGVPLGMAEIATALYARHLRFHSADPTWPDRD 60 M HPLQPLAHAIRFLSIDAIVRATEGHQGVPLGMAEIATALYARHLRF+ A PTW DRD Sbjct: 1 MNEHPLQPLAHAIRFLSIDAIVRATEGHQGVPLGMAEIATALYARHLRFNPAAPTWWDRD 60 Query: 61 RVVLSNGHGSMLLYSLLYLTGYEHITLDQVRNFREFGSHCAGHPEYDPQLGIEVTTGPLG 120 RVVLSNGHGSMLLY LLYLTGYEHITL+QVR+FREFGSHCAGHPEY P+LGIEVTTGPLG Sbjct: 61 RVVLSNGHGSMLLYCLLYLTGYEHITLEQVRSFREFGSHCAGHPEYAPELGIEVTTGPLG 120 Query: 121 QGIANAFGMAVAEAYLNARFGDGIVNHRTYAFVGDGCLQEGIGQEMISLAGHLRLAKLTL 180 QGIANAFGMAVAEAYLNARFGD IVNHRTYAFVGDGCLQEGIGQEMISLAGHLRL KLTL Sbjct: 121 QGIANAFGMAVAEAYLNARFGDDIVNHRTYAFVGDGCLQEGIGQEMISLAGHLRLHKLTL 180 Query: 181 LWDDNQITDDGATELSISENVPERFRVAGWHVVEVDGHDIEAVSRALDAARASDRPSMIA 240 LWDDNQITDDG TELSISENV ERFRVAGWHVVEVDGHDI+AVSRALDAA+ SDRPSMIA Sbjct: 181 LWDDNQITDDGKTELSISENVAERFRVAGWHVVEVDGHDIQAVSRALDAAQGSDRPSMIA 240 Query: 241 CRTIIGRGIPRVQGQRGGHSARLHAEDAQAARDLLQWPHAPFEIPQDILTPWRNFGQRNR 300 CRT+IGRGIPRVQGQRGGHSARL+ +DAQAAR+LLQWPHAPF++PQDIL WR+FGQRN Sbjct: 241 CRTVIGRGIPRVQGQRGGHSARLYEDDAQAARELLQWPHAPFDVPQDILAQWRSFGQRND 300 Query: 301 DEHAAWHQRLQALPPTQRAEFERVLRGDLPDGWQDVLLQFKRTAHEPAQAQGGILISAAL 360 +H W R+QALP QRAEFERV+RG+LP GWQ+VLL FKR+AHEP QAQGGILISAA+ Sbjct: 301 AQHQQWQARVQALPAAQRAEFERVMRGELPSGWQEVLLPFKRSAHEPEQAQGGILISAAI 360 Query: 361 NQLLAEVIPERMVGCADLEAPTAHKGSLKAFTASDRSGAYVHCGVREHVMGAMANGMAAH 420 N+LLAEV+PERMVGCADLEAPTAHKG L AFTA DRSGAYVHCGVREH+MGAMANGMAAH Sbjct: 361 NKLLAEVMPERMVGCADLEAPTAHKGGLHAFTADDRSGAYVHCGVREHLMGAMANGMAAH 420 Query: 421 GGVIPLAVTYLAFSDYERPAMRMAALMGLPVKFVFSHDSIGIGKNGPTHQPVEILASLRA 480 GGVI L+VTYLAFSDYERPAMRM+ALMGLPVK+VFSHDSIGIGKNGPTHQPVEILASLRA Sbjct: 421 GGVIALSVTYLAFSDYERPAMRMSALMGLPVKYVFSHDSIGIGKNGPTHQPVEILASLRA 480 Query: 481 MPNMLVMRPADASEAVECWEVAMQHRSGPVSLVFARQSLPPVRKRFEVSNLSARGGYVLE 540 MPNMLVMRPADA+EAVECWE+AMQHRSGPVS+VFARQ+LPPVR+++E N ARGGYVL Sbjct: 481 MPNMLVMRPADATEAVECWEIAMQHRSGPVSMVFARQALPPVRRQYEAENRCARGGYVLH 540 Query: 541 EAQCGPRQVSLLATGSEVAIACAARDLLEARGVATAVVSLPCWELFDQQDSRYRDAVLGE 600 EAQCG RQV+LLATGSEVA+ACAAR LEA G+ATAVVSLPCWELFDQQD+ YR VLGE Sbjct: 541 EAQCGARQVTLLATGSEVALACAARAQLEAAGIATAVVSLPCWELFDQQDTAYRQQVLGE 600 Query: 601 DTVRVGVEAAVRFGWDRYLGARGGFVGMVGFGAPGPADVLYRHFGITPEAVVAQALQLL 659 TVRVGVEAA+RFGWD YLGARGGFVGM GFGAPGPADVLYRHF IT EAVVA+A +LL Sbjct: 601 QTVRVGVEAALRFGWDHYLGARGGFVGMPGFGAPGPADVLYRHFNITAEAVVAEARRLL 659 >gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 566 bits (1458), Expect = e-162, Method: Compositional matrix adjust. Identities = 299/663 (45%), Positives = 417/663 (62%), Gaps = 13/663 (1%) Query: 5 PLQPLAHAIRFLSIDAIVRATEGHQGVPLGMAEIATALYARHLRFHSADPTWPDRDRVVL 64 P +A+AIR L++DA+ +A GH G+P+GMAEIA AL+A+H R + A+P W +RDR VL Sbjct: 6 PTDKMANAIRALAMDAVQKANSGHPGMPMGMAEIAVALWAKHYRHNPANPHWANRDRFVL 65 Query: 65 SNGHGSMLLYSLLYLTGYEHITLDQVRNFREFGSHCAGHPEYDPQLGIEVTTGPLGQGIA 124 SNGHGSML Y+LL+LTGY+ ++++ +++FR+ S AGHPE G+E TTGPLGQGI Sbjct: 66 SNGHGSMLQYALLHLTGYD-LSMEDIKSFRQLHSKTAGHPEVHITPGVETTTGPLGQGIT 124 Query: 125 NAFGMAVAEAYLNARF---GDGIVNHRTYAFVGDGCLQEGIGQEMISLAGHLRLAKLTLL 181 NA GMA+AE L A F G +V+H TYAFVGDGCL EGI E SLAG L L+KL +L Sbjct: 125 NAVGMALAEKLLAAEFNKPGLAVVDHYTYAFVGDGCLMEGISHEACSLAGVLNLSKLIVL 184 Query: 182 WDDNQITDDGATELSISENVPERFRVAGWHVV-EVDGHDIEAVSRALDAARASDRPSMIA 240 +DDN I+ DG E ++ P+RF GW+V+ VDGH +EAVS A+ A+ +D+P++I Sbjct: 185 YDDNGISIDGHVEGWFKDDTPKRFEAYGWNVIPAVDGHSVEAVSAAIHQAKLADKPTLIC 244 Query: 241 CRTIIGRGIPRVQGQRGGHSARLHAEDAQAARDLLQWPHAPFEIPQDILTPWRNFGQRNR 300 CRT+IG+G P + G H A L ++ A R+ L W APFEIP D+ + W + Sbjct: 245 CRTVIGKGSPNLAGTDKVHGAALGDKEIAAVREALGWTSAPFEIPADVYSAW-DAKATGA 303 Query: 301 DEHAAWHQRLQ---ALPPTQRAEFERVLRGDLPDGWQDVLLQFKRTAHEPAQAQGGILIS 357 D A+W Q A P++ AE R L+G+LP ++ + + + E + S Sbjct: 304 DFEASWKQTFDTYAAQYPSEAAELLRRLKGELPAAFESTVTAYINSTLEKKETIATRKAS 363 Query: 358 AALNQLLAEVIPERMVGCADLEAPTAHKGSLKAFTASDRSGAYVHCGVREHVMGAMANGM 417 Q A+ +PE + G ADL +D+ G +++ GVRE M A+ NG+ Sbjct: 364 QNAIQAYAQALPEFLGGSADLTGSNLTNWKESVAVRADQRGNHINYGVREFGMSAIMNGI 423 Query: 418 AAHGGVIPLAVTYLAFSDYERPAMRMAALMGLPVKFVFSHDSIGIGKNGPTHQPVEILAS 477 A HGG +P T+L FSDY R A+RMAALM + FVF+HDSIG+G++GPTHQ VE ++S Sbjct: 424 ALHGGYLPFGATFLTFSDYSRNALRMAALMKIRALFVFTHDSIGLGEDGPTHQSVEHVSS 483 Query: 478 LRAMPNMLVMRPADASEAVECWEVAMQHRSGPVSLVFARQSLP-PVRKRFEVSNLSARGG 536 LR +PN+ RP D +E W +++ GP +L+F+RQ+LP R +V ++ ARGG Sbjct: 484 LRLIPNLDNWRPCDTTETAVAWAESIKRHDGPSTLIFSRQNLPFQERTDAQVKDI-ARGG 542 Query: 537 YVLEEAQCGPRQVSLLATGSEVAIACAARDLLEARGVATAVVSLPCWELFDQQDSRYRDA 596 YVL +A +V L+ATGSE+ +A A D L A G+A VVS+P ++FD+QD+ Y+ + Sbjct: 543 YVLRDAPNA--KVILMATGSEIELATKAADALTAEGIAVRVVSMPSTDVFDRQDAAYKAS 600 Query: 597 VLGEDTVRVGVEAAVRFGWDRYLGARGGFVGMVGFGAPGPADVLYRHFGITPEAVVAQAL 656 VL RV +EA V W +Y+G G VG+ FG PA VL++HFG T E VVA+A Sbjct: 601 VLTRGVPRVAIEAGVTAFWHKYVGLEGAVVGIDTFGESAPAGVLFKHFGFTVENVVAKAK 660 Query: 657 QLL 659 +L Sbjct: 661 AVL 663 >gi|300309645|ref|YP_003773737.1| transketolase N-terminal subunit [Herbaspirillum seropedicae SmR1] Length = 272 Score = 117 bits (292), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 88/236 (37%), Positives = 119/236 (50%), Gaps = 16/236 (6%) Query: 23 RATEGHQGVPLGMAEIATALY-ARHLRFHSADPTWPDRDRVVLSNGHGSMLLYSLLYLTG 81 RA E L +I ALY LRF+ ADP RDR V+S GHGS+ +Y +L G Sbjct: 25 RAPETRLASSLSSIDIFVALYYGGVLRFNPADPRDAGRDRCVISKGHGSICMYPILADLG 84 Query: 82 YEHITLDQVRNFREFGSHCAGHPEYDPQL-GIEVTTGPLGQGIANAFGMAVAEAYLNARF 140 Y ++++++ E GS G P DP + G E G LG G+ A GMA+ R Sbjct: 85 Y--FPMEELQHVCEAGSFLGGIP--DPVIPGYETVNGSLGHGLGVATGMALGL----KRS 136 Query: 141 GDGIVNHRTYAFVGDGCLQEGIGQEMISLAGHLRLAKLTLLWDDNQITDDGATELSISE- 199 G + + GDG L EG E I A +L L L+ DDN+I+ G TE +S Sbjct: 137 GS---DRSVFVVAGDGELHEGANWEAIMFASQHKLDNLHLIVDDNRISMLGYTEDIVSHG 193 Query: 200 NVPERFRVAGWHVVEVDGHDIEAVSRAL--DAARASDRPSMIACRTIIGRGIPRVQ 253 + R GW +EVDGHD+ AV AL A A+ +P I RT+ G G+P ++ Sbjct: 194 ELATRLSAFGWDCMEVDGHDVLAVQAALLQQKAHAAGKPKAIIARTLKGHGVPGLE 249 >gi|300310439|ref|YP_003774531.1| transketolase alpha subunit [Herbaspirillum seropedicae SmR1] Length = 281 Score = 106 bits (264), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 81/258 (31%), Positives = 125/258 (48%), Gaps = 22/258 (8%) Query: 26 EGHQGVPLGMAEIATALYARHLRFHSADPTWPDRDRVVLSNGHGSMLLYSLLYLTGYEHI 85 +G+ G LG A++ Y + DP W RDR +LS+GH ++ LY+ L G I Sbjct: 28 QGYIGQALGWADVLAVAYKHAMSLRPDDPQWSGRDRFLLSHGHYAIALYAALIEAG---I 84 Query: 86 TLDQVRNFREFGSH-----CAGHPEYDPQLGIEVTTGPLGQGIANAFGMAVAEAYLNARF 140 D+ +GS +G Y P G+E++ G LGQG+A GMA+ Y N Sbjct: 85 VPDE--ELDSYGSDDSRLPMSGMATYTP--GMEISGGSLGQGLAIGVGMALGLRYNNNP- 139 Query: 141 GDGIVNHRTYAFVGDGCLQEGIGQEMISLAGHLRLAKLTLLWD-DNQITDDGATELSISE 199 Y + DG L EG E A H +L L L D +NQ D + ++ E Sbjct: 140 ------AFVYNSMSDGELDEGSTWEAAMGAAHHQLGNLICLVDINNQQADGPSGKIMGFE 193 Query: 200 NVPERFRVAGWHVVEVDGHDIEAVSRALDAAR--ASDRPSMIACRTIIGRGIPRVQGQRG 257 + +++ GW+V VDG+D+ AV +A D AR +P +I T++G+G+P ++ + Sbjct: 194 PLADKWAAFGWYVQRVDGNDLAAVVQAFDNARNLKDAKPRVILFDTLMGKGVPFLEQREK 253 Query: 258 GHSARLHAEDAQAARDLL 275 H R+ + Q A +L Sbjct: 254 NHFIRVDPPEWQQALSIL 271 >gi|300310438|ref|YP_003774530.1| transketolase, C-terminal subunit [Herbaspirillum seropedicae SmR1] Length = 334 Score = 61.2 bits (147), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 61/215 (28%), Positives = 97/215 (45%), Gaps = 16/215 (7%) Query: 400 YVHCGVREHVMGAMANGMAAHGGVIPLAVTYLAFSDYERPAM--RMAALMGLPVKFVFSH 457 + G+ E ++ A A GMA G ++P A TY F+ ++ A L VK + Sbjct: 70 FFQMGMAEQLLMAAAGGMAKEG-LVPFATTYAVFATRRAYDFIHQVIAEENLNVKICCAL 128 Query: 458 DSIGIGKNGPTHQPVEILASLRAMPNMLVMRPADASEAVECWEVAMQHRSGPVSLVFARQ 517 + G GP+HQ E LA +RA+P M ++ P DA + + H+ GPV + AR Sbjct: 129 PGLTTG-YGPSHQATEDLAMMRAIPGMTIVDPCDALDLEQAVPQIAAHQ-GPVYMRLARG 186 Query: 518 SLPPVRKRFEVSNLSARGGYVLEEAQCGPRQVSLLATGSEVAIACAARDLLEARGVATAV 577 ++P V ++ R +E + G V ++++G A LEA V+ AV Sbjct: 187 NVPLVLDEYDYRFELGRA----KEIRDG-NDVLIISSGFMTMRALEVAKALEADKVSVAV 241 Query: 578 VSLPCWELFD------QQDSRYRDAVLGEDTVRVG 606 + P + FD Q + R V+ E+ VG Sbjct: 242 LHCPTIKPFDEATVVAQVKKKNRLVVVAENHSVVG 276 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,175,531 Number of extensions: 95735 Number of successful extensions: 306 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 294 Number of HSP's successfully gapped: 5 Length of query: 667 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 575 Effective length of database: 1,184,250 Effective search space: 680943750 Effective search space used: 680943750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)