BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0179.p (975 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309839|ref|YP_003773931.1| metal-dependent phosphohydrolas... 1508 0.0 gi|300309652|ref|YP_003773744.1| HD-GYP domain-containing protei... 153 7e-38 gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protei... 71 4e-13 gi|300310565|ref|YP_003774657.1| response regulator containing a... 70 8e-13 gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent p... 70 1e-12 >gi|300309839|ref|YP_003773931.1| metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 975 Score = 1508 bits (3903), Expect = 0.0, Method: Compositional matrix adjust. Identities = 760/968 (78%), Positives = 833/968 (86%), Gaps = 19/968 (1%) Query: 8 LFDPSDDDATVPGLPVRFGISALVMAGMVLLAVVLVSVGWWGARQVLLDMAERSAEDAGR 67 L PS D + GLPVRFGISALVMAGM+LLAVVLV+VGWWGARQVLLDMAERSA+DAGR Sbjct: 15 LHAPSRDLRST-GLPVRFGISALVMAGMILLAVVLVTVGWWGARQVLLDMAERSAQDAGR 73 Query: 68 IMTERSRRIIDPAVATLRQLAFDPITQATQLDERLTRIAAFSIDLVANPLVSAVYVGYPN 127 IMTERSRRIIDPAVATLRQLAFDPITQA +LD RL RIAAFS DLVANPLVSAVYVGYPN Sbjct: 74 IMTERSRRIIDPAVATLRQLAFDPITQAGELDTRLARIAAFSADLVANPLVSAVYVGYPN 133 Query: 128 GDFLLVRSLRGADQRAFFSAPAASTFLVQSIVRDADGTQHGQYRFYDAELKLLALRDEAD 187 GDFLLVR+LRGA+QR+FF AP ++ FLVQ+I R+A+G +HGQYRFYD +L+LL+LR++ + Sbjct: 134 GDFLLVRALRGAEQRSFFRAPESARFLVQTITRNANGQEHGQYRFYDDDLRLLSLREQPE 193 Query: 188 YLFDPRTRPWYATADRSLVPSISAPYVFFTTRQIGISLSQRARDGEAVVAIDMTLDDLAA 247 Y FDPRTRPWYA+A+R+L+PSISAPYVFFTTRQIGISLSQRA EAVVAIDMTLDDLA Sbjct: 194 YHFDPRTRPWYASANRALMPSISAPYVFFTTRQIGISLSQRAHASEAVVAIDMTLDDLAT 253 Query: 248 SLGNLRLTPNSELALVDEHQRVIAYSDMARVLEQEGESFRFRSLKDLEVPSLSQLSARAL 307 SLGNLRLTP+SELALVDE +VIAYSDM RVLEQ+G+SFRFRSL +L P+LS L+ RA Sbjct: 254 SLGNLRLTPHSELALVDEAGKVIAYSDMNRVLEQDGDSFRFRSLDELNFPTLSLLARRAR 313 Query: 308 AGDTSALYSVNGREWLGLSLPFDVIDGHALRLLIAAPGDELLGQLDRNRKTLVLVAFLVV 367 +G T+ALY G+EWLGLSLPF+VID H L LLIAAPGDELLGQL+RNRKT+VL+A +V Sbjct: 314 SGQTNALYHAGGQEWLGLSLPFNVIDDHPLHLLIAAPGDELLGQLERNRKTMVLIALFLV 373 Query: 368 LAFLPLGWWAGRAIGRSMERLAVRAGRMSRFDFRTQDRRRSTLREINMLTDMVDHVSLTV 427 +AFLPLGWWAGRAIGRSMERLA RA RMSRFDFR Q + S LRE+NMLT+MVD+VSLTV Sbjct: 374 IAFLPLGWWAGRAIGRSMERLAARARRMSRFDFREQGKTASMLREVNMLTEMVDNVSLTV 433 Query: 428 EAFLDISAILSTETHVEEMLQQVLEKFVQATRCDGGAVYLWQREDGQMQRAAAYGQQCGL 487 EAFLDISAILSTETHVE+MLQQVLEKF+QATRC GAVYLWQRE GQMQRAA YG GL Sbjct: 434 EAFLDISAILSTETHVEQMLQQVLEKFIQATRCQAGAVYLWQRESGQMQRAAGYGADHGL 493 Query: 488 TDSMAYDAQGTGAGAADTIAARETDDGRMRVELDLRGRNGSLEGLLVLMHAHDSVHEDPR 547 +S A+D Q T + R DG +RVELDLRGRNGSLEGLLVL+HAHDS H DPR Sbjct: 494 AESFAHDRQHTPSS-----NTRALPDGSLRVELDLRGRNGSLEGLLVLVHAHDSAHGDPR 548 Query: 548 FLAFARRLSGMLAVAIETRQLIAAQKALLESLIRLMADAIDAKSPYTGGHCERVPHLAIQ 607 FLAFARRLSGMLAVAIETRQLIAAQKALLESLIRLMADAIDAKSPYTGGHCERVPHLAI Sbjct: 549 FLAFARRLSGMLAVAIETRQLIAAQKALLESLIRLMADAIDAKSPYTGGHCERVPHLAIM 608 Query: 608 LVDRLAQESEGRYAGFTLNEDQRYAFQLAALLHDCGKVTSPEHIVDKATKLEVIHNRIHE 667 LV+RLAQE EG YAGF+LNED+RYAF+LAALLHDCGKVTSPEHIVDKATKLEVIHNRIHE Sbjct: 609 LVERLAQEREGPYAGFSLNEDERYAFRLAALLHDCGKVTSPEHIVDKATKLEVIHNRIHE 668 Query: 668 VRMRFEVLWRDAELDLLRELYATLPAALAESWRAELERRRHQLREDFAFVAQCNVGGEAM 727 VRMRFEVLWRDAEL LL + LP AL+E LE +R +LREDFAFVAQCNVG EAM Sbjct: 669 VRMRFEVLWRDAELALLHQ----LPQALSEQQAQALEAQRARLREDFAFVAQCNVGSEAM 724 Query: 728 SEEAIARLRQIAMQTWQRHFDDSLG------LADEESRRLAAAAIAHXPPDTETLLADKP 781 SEEAIARL +IA QTW+RHFDDSLG +R+ AA + P TE LLAD+P Sbjct: 725 SEEAIARLARIATQTWERHFDDSLGLAAEEARRLAATRQGQAAQVL---PVTEPLLADRP 781 Query: 782 EHKVAWEDGHRPPVERGDPRNVLGFDMQLPQVRQNMGELHNLTVRRGTLTEEDRFAINNH 841 EH V W++ HRP VERGDPRN LGFDM LP+ RQNMGELHNL +RRGTLT EDRFAINNH Sbjct: 782 EHIVPWDEEHRPAVERGDPRNTLGFDMTLPRYRQNMGELHNLGIRRGTLTPEDRFAINNH 841 Query: 842 IVQTLVMLKSLPWPDSLKSVPDTAANHHEKMDGTGYPRRLRASGLPLQDRVMALADVFEA 901 IVQTLVMLKSLPWP+SLKSVPDTAANHHEKMDGTGYPRRL+AS LPLQDRVMALADVFEA Sbjct: 842 IVQTLVMLKSLPWPESLKSVPDTAANHHEKMDGTGYPRRLKASELPLQDRVMALADVFEA 901 Query: 902 LTAADRPYKPAKTLSESLRIMARMCREQHLDTELFRYFLHSGLWRTYADELLRPGQRDAV 961 LTAADRPYKPAKTLSESLRIMA MCRE+HLDTELFRYFL S LW YA ELL+ GQ DAV Sbjct: 902 LTAADRPYKPAKTLSESLRIMANMCRERHLDTELFRYFLRSRLWEVYAGELLKEGQIDAV 961 Query: 962 DIAAIEDL 969 DI IE L Sbjct: 962 DIVEIEAL 969 >gi|300309652|ref|YP_003773744.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 552 Score = 153 bits (386), Expect = 7e-38, Method: Compositional matrix adjust. Identities = 98/226 (43%), Positives = 130/226 (57%), Gaps = 8/226 (3%) Query: 527 GSLEGLLVLMHAHDSVHEDPRFLA-----FARRLSGMLAVAIETRQLIAAQKALLESLIR 581 G L G+L L++A D P + F L+ A+A+ +QLI + L ESLIR Sbjct: 161 GELVGVLQLINAIDPATGAPLPFSETDQHFIEALASQAAMAMTNQQLIQRLEQLFESLIR 220 Query: 582 LMADAIDAKSPYTGGHCERVPHLAIQLVDRLAQESEGRYAGFTLNEDQRYAFQLAALLHD 641 L+ I KSP+TG HCE+VP LA+ L + +EG A F ++E R LAALLHD Sbjct: 221 LINIGIGEKSPHTGRHCEQVPELAMMLAEAAHNATEGPLADFHMSEADRRELWLAALLHD 280 Query: 642 CGKVTSPEHIVDKATKLEVIHNRIHEVRMRFEVLWRDAELDLLRE---LYATLPAALAES 698 CGK+++P HIV+KATKLE I +RIH V R EVL RDAE+ +L+E L A L A A Sbjct: 281 CGKISTPTHIVEKATKLETIFDRIHLVETRIEVLKRDAEIRVLQEKLALGAALDAQRAAE 340 Query: 699 WRAELERRRHQLREDFAFVAQCNVGGEAMSEEAIARLRQIAMQTWQ 744 A L QL + AF+ + NVG E M E R+ IA ++W+ Sbjct: 341 LDAGLAAELAQLDLERAFLRKINVGTEGMRPEDQQRVLDIAQRSWR 386 Score = 149 bits (377), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 69/145 (47%), Positives = 97/145 (66%) Query: 822 NLTVRRGTLTEEDRFAINNHIVQTLVMLKSLPWPDSLKSVPDTAANHHEKMDGTGYPRRL 881 NL +R GTL + +R INNHIV T+ ML+SLPWP L V + A HHE+MDG GYP+ L Sbjct: 403 NLRIRAGTLNDAERQIINNHIVITIKMLESLPWPKQLARVTEFAGGHHERMDGKGYPKGL 462 Query: 882 RASGLPLQDRVMALADVFEALTAADRPYKPAKTLSESLRIMARMCREQHLDTELFRYFLH 941 + +Q R+MA+AD+FEA+TAADRPYK TL+E++ I+A H+D +LF F+ Sbjct: 463 TREQMSVQARIMAVADIFEAVTAADRPYKRGNTLNEAVEILAGFKARDHIDPDLFDLFVR 522 Query: 942 SGLWRTYADELLRPGQRDAVDIAAI 966 ++ YA++ + P Q D VD++ I Sbjct: 523 ERIYEKYAEKFMHPSQIDEVDLSRI 547 >gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 71.2 bits (173), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 52/165 (31%), Positives = 88/165 (53%), Gaps = 17/165 (10%) Query: 804 LGFDMQLPQVRQNMGELHN---LTV------RRGTLTEEDRFAINNHIVQTLVMLKSLPW 854 LG Q Q G LH+ +TV + LTEE+ ++ +H + +L+ LP Sbjct: 183 LGLSAQERQQAGMAGLLHDIGKMTVPPSILNKPSKLTEEEFVSVQSHPLAGHRLLQELP- 241 Query: 855 PDSLKSVP-DTAANHHEKMDGTGYPRRLRASGLPLQDRVMALADVFEALTAADRPYKPAK 913 + P D + +HHEKMDGTGYP +L + + R+ A+ DV++A+T +DRPYK Sbjct: 242 --GIGDAPLDVSLHHHEKMDGTGYPYKLSGRDISMTARMGAICDVYDAIT-SDRPYKSGW 298 Query: 914 TLSESLRIMARMCREQHLDTELFRYFLHS-GLWRTYADELLRPGQ 957 S S++ MA+ + H D ++ + F+ + G++ + L++ G Sbjct: 299 DPSRSIQHMAQ--SKGHFDFDVLQAFVKAVGIYPVGSCVLMQTGH 341 >gi|300310565|ref|YP_003774657.1| response regulator containing a CheY-like receiver domain and an HD-GYP domain [Herbaspirillum seropedicae SmR1] Length = 348 Score = 70.1 bits (170), Expect = 8e-13, Method: Compositional matrix adjust. Identities = 48/149 (32%), Positives = 75/149 (50%), Gaps = 18/149 (12%) Query: 804 LGFDMQLPQVRQNMGELHNL---------TVRRGTLTEEDRFAINNHIVQTLVMLKSLPW 854 +G D Q ++ N +H++ ++ G L EE+ + H +L + P Sbjct: 189 MGLDSQQQELLLNAAPMHDIGKMGIPDHILLKPGRLDEEELKVMRMHARIGADILVASPS 248 Query: 855 PDSLKSVPDTAANHHEKMDGTGYPRRLRASGLPLQDRVMALADVFEALTAADRPYKPAKT 914 P L++ A HHEK DG+GYP L+ +PL R++A+ADVF+ALT++ RPYKP + Sbjct: 249 P-LLQTASQIALTHHEKWDGSGYPNGLKGEQIPLYGRIVAVADVFDALTSS-RPYKPGWS 306 Query: 915 LSESLRIMARMCREQ---HLDTELFRYFL 940 L + ++ REQ H D F Sbjct: 307 LERA----SQYLREQSGAHFDPRCVEAFF 331 >gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 69.7 bits (169), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 46/126 (36%), Positives = 65/126 (51%), Gaps = 13/126 (10%) Query: 821 HNLTVRRGTLTEEDRFAINNHIVQTLVMLKSLPWPDSLKSVPDTA----ANHHEKMDGTG 876 H L + G L++E+ I H L P +VPD A +HHEK+DG G Sbjct: 203 HELLNKPGKLSDEEFSQIKKHPELGYKYLSQDP------NVPDYARDVCLHHHEKIDGNG 256 Query: 877 YPRRLRASGLPLQDRVMALADVFEALTAADRPYKPAKTLSESLRIMARMCREQHLDTELF 936 YP LRA + L R+ A+ DV++ALT +DRPYK A +E++ MA H D +F Sbjct: 257 YPEGLRAGAITLYSRMAAICDVYDALT-SDRPYKRAWDPAEAISAMASWT--GHFDRAMF 313 Query: 937 RYFLHS 942 F+ + Sbjct: 314 LAFVKT 319 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,939,296 Number of extensions: 120333 Number of successful extensions: 432 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 425 Number of HSP's successfully gapped: 12 Length of query: 975 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 881 Effective length of database: 1,174,780 Effective search space: 1034981180 Effective search space used: 1034981180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 135 (56.6 bits)