BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0175.p (228 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310791|ref|YP_003774883.1| solute binding periplasmic prot... 92 3e-20 >gi|300310791|ref|YP_003774883.1| solute binding periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 262 Score = 92.0 bits (227), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 73/234 (31%), Positives = 116/234 (49%), Gaps = 16/234 (6%) Query: 1 VLAAGAIAPIVRTVAADFERRSGIHVTVDN------ATGGVVDKRVRSGAAADVVVAPDG 54 V+++G A +T+A +FE+++G H + T + R++ G DVV+ Sbjct: 33 VVSSGGFAAAYKTLAPEFEKKTG-HKLISGWGPSMGETPQAIPNRLQRGEHIDVVIMVGD 91 Query: 55 VLRGLGKDGYLNLASERPIARVGIAVAVRQGAPSPAIATVAQFRKALLDAPSVAVIDPKA 114 L L G ++ + +A I +AV+ GAP P I+ + F++ LL A SV D Sbjct: 92 SLDKLVAAGKVSKTEHKLLALSRIGLAVKAGAPRPDISNLDAFKRTLLTAHSVVYSD--- 148 Query: 115 GGSSGIYLE-KLFERLGIASQMHAKEVLVPGGLVAEKLVDGSAALALHQQSEILLVKGAK 173 +SG++L KLF+RLGI QM K ++P V + + G A + L Q SE+ VKG Sbjct: 149 -SASGVFLSTKLFKRLGIDQQMAYKGRMIPAEPVGQVVARGDAEIGLQQISELKPVKGID 207 Query: 174 LVGPLPADIQNYTVYVG--AVGARTASGATGRAFLTMFGEETARRAMVEHGLLP 225 ++GP+P + Q T + VGA A A + + A+ A+ GL P Sbjct: 208 IIGPIPEEAQQLTPFSAGVVVGAHEAEAA--KELVHFLASPEAQAAIKASGLDP 259 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 646,272 Number of extensions: 25223 Number of successful extensions: 105 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 105 Number of HSP's successfully gapped: 1 Length of query: 228 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 146 Effective length of database: 1,231,600 Effective search space: 179813600 Effective search space used: 179813600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)