BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0150.p (479 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 759 0.0 gi|300313894|ref|YP_003777986.1| RND family efflux transporter M... 357 1e-99 gi|300310689|ref|YP_003774781.1| RND family efflux transporter M... 344 9e-96 gi|300310851|ref|YP_003774943.1| RND family efflux transporter M... 162 4e-41 gi|300309658|ref|YP_003773750.1| HlyD-family secretion protein [... 145 5e-36 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 759 bits (1960), Expect = 0.0, Method: Compositional matrix adjust. Identities = 384/402 (95%), Positives = 394/402 (98%) Query: 78 MSSVSRFTRLTMTVAVAAALVACGQKPAAQGGQMPPAEVSVVTIAPQRVAITNELPGRVE 137 MSSVSRFTRLTMTVA+AAALVACGQKPAAQGG MPP+EVSVVTIAPQRVAITNELPGRVE Sbjct: 1 MSSVSRFTRLTMTVAIAAALVACGQKPAAQGGPMPPSEVSVVTIAPQRVAITNELPGRVE 60 Query: 138 AFRVAQVRARVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQ 197 AFRVAQVRARVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQ Sbjct: 61 AFRVAQVRARVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQ 120 Query: 198 TSLTAQRYKPLVEVNAVSKQEYDNAVAAQKQAEADVAAGKAAVQTANLNLGYATVTSPIS 257 T+LTAQRYKPLVEVNAVSKQEYDNAVAAQKQAEADVAAGKAAVQTA+LNLGYATVTSPIS Sbjct: 121 TTLTAQRYKPLVEVNAVSKQEYDNAVAAQKQAEADVAAGKAAVQTASLNLGYATVTSPIS 180 Query: 258 GRIGRALVTEGALVGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQLKSVGDN 317 GRIGRALVTEGALVGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQLKSVGDN Sbjct: 181 GRIGRALVTEGALVGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQLKSVGDN 240 Query: 318 KASVTLVLENGQAYDQPGKLLFSDISVDESTGSVSLRAEFPNPKRLLLPGTYVRTKIEQA 377 KASVTL LENGQAY PGKLLFSDISVDE+TGSVSLRAEFPNPKRLLLPGTYVR KIEQA Sbjct: 241 KASVTLELENGQAYPLPGKLLFSDISVDENTGSVSLRAEFPNPKRLLLPGTYVRAKIEQA 300 Query: 378 VDEQALLVPQQAVVRDAGGSSVMVVNAESKVEARPVKTGGARGNNWQVIDGLKEGDRVIV 437 VDEQALLVPQQAVVRDAGGS+VMVVNAE+KVE RPVKTGGARGNNWQV+DGLKEGDRVIV Sbjct: 301 VDEQALLVPQQAVVRDAGGSTVMVVNAENKVEPRPVKTGGARGNNWQVLDGLKEGDRVIV 360 Query: 438 EGLQKVRPGAAVKPVQWNPAGAPAQAAGQPAKQDASAQQKAN 479 EGLQKVRPGA VKPVQWNPAGAPAQA+GQPAKQ+ASAQ KAN Sbjct: 361 EGLQKVRPGATVKPVQWNPAGAPAQASGQPAKQEASAQPKAN 402 >gi|300313894|ref|YP_003777986.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 428 Score = 357 bits (916), Expect = 1e-99, Method: Compositional matrix adjust. Identities = 187/373 (50%), Positives = 258/373 (69%), Gaps = 12/373 (3%) Query: 86 RLTMTVAVAAALVACGQ-----KPAAQGGQMPPAEVSVVTIAPQRVAITNELPGRVEAFR 140 RL +++ + A L AC + KP AQ AEV V + QR A+T ELPGR A+ Sbjct: 20 RLALSLILVAVLAACSRSGDQPKPTAQ------AEVGYVVVKAQRQALTTELPGRTTAYL 73 Query: 141 VAQVRARVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQTSL 200 A +R +V GI+QKR+FKEGS VK GQ L++IDPA Y+A+ +SA+A+L +A+A L + L Sbjct: 74 AADIRPQVGGIIQKRLFKEGSTVKAGQPLYQIDPASYQAAYDSARASLLKAEATLASSKL 133 Query: 201 TAQRYKPLVEVNAVSKQEYDNAVAAQKQAEADVAAGKAAVQTANLNLGYATVTSPISGRI 260 A+RY L +++AVSKQ+ D+A + +Q +ADV KAAVQTA +NL Y + SPISGRI Sbjct: 134 KAERYAELNKIDAVSKQDNDDAQSTLRQNQADVEVQKAAVQTAAINLQYTKILSPISGRI 193 Query: 261 GRALVTEGALVGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQLKSVGDNKAS 320 ++ VT GALV T L VQQ+DP+YV+VTQS+ DLL+L++ +A+GQ++ G+N A Sbjct: 194 EKSAVTPGALVTASQTTALTTVQQIDPIYVDVTQSTVDLLRLRRELASGQIRKSGENAAQ 253 Query: 321 VTLVLENGQAYDQPGKLLFSDISVDESTGSVSLRAEFPNPKRLLLPGTYVRTKIEQAVDE 380 V +VLE+G Y++PGKL F+ +SVD TG ++LRA PNP +LLPG YVR +E+ VD+ Sbjct: 254 VKIVLEDGSTYERPGKLEFTGLSVDTGTGMITLRAVVPNPDGVLLPGAYVRAVLEEGVDD 313 Query: 381 QALLVPQQAVVRD-AGGSSVMVVNAESKVEARPVKTGGARGNNWQVIDGLKEGDRVIVEG 439 ALL PQ+AV RD G ++ +V+ A+ KV+ R V A GNNW V GLKEGD++IV+G Sbjct: 314 HALLAPQKAVSRDQTGAATALVLGADGKVQQRLVSVARAVGNNWWVSRGLKEGDKLIVDG 373 Query: 440 LQKVRPGAAVKPV 452 LQKVR G A + + Sbjct: 374 LQKVRTGDAPRAI 386 >gi|300310689|ref|YP_003774781.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 400 Score = 344 bits (882), Expect = 9e-96, Method: Compositional matrix adjust. Identities = 184/367 (50%), Positives = 251/367 (68%), Gaps = 2/367 (0%) Query: 91 VAVAAALVACGQKPAAQGGQMPPAEVSVVTIAPQRVAITNELPGRVEAFRVAQVRARVAG 150 +A+ + L ACG+ P + VVT+ Q V + ELPGR +++A VR +V G Sbjct: 16 LAILSVLSACGKPPGGAPPAAGTPVLGVVTVHAQPVELHTELPGRTSPYQIADVRPQVNG 75 Query: 151 IVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQTSLTAQRYKPLVE 210 I++ R F+EGS+VK GQVL++I+PA Y+A+ +S+ A LA+A+A++ L A RYK LV Sbjct: 76 IIKARSFREGSDVKAGQVLYQINPASYQAAYDSSVAALAKAEASVRTARLKASRYKELVA 135 Query: 211 VNAVSKQEYDNAVAAQKQAEADVAAGKAAVQTANLNLGYATVTSPISGRIGRALVTEGAL 270 + AVS+Q+YD+AVA+ +AEADV + +A VQT+ +NL YA + +PISGRIG++ VT GAL Sbjct: 136 IKAVSQQDYDDAVASLGEAEADVGSARANVQTSRINLDYARLDAPISGRIGKSSVTPGAL 195 Query: 271 VGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQLKSVGDNKASVTLVLENGQA 330 V T L +QQLDP+YV+VTQSS L L++A+A GQL+ G N A V LVLENG+ Sbjct: 196 VTANQTTALTTIQQLDPIYVDVTQSSTTWLNLKEALANGQLEKSG-NGAKVRLVLENGRT 254 Query: 331 YDQPGKLLFSDISVDESTGSVSLRAEFPNPKRLLLPGTYVRTKIEQAVDEQALLVPQQAV 390 Y Q G L FSD++V++ TG+++LRA FPNPK LLPG YVR +++ V +Q LLVPQQAV Sbjct: 255 YAQEGTLQFSDVTVNQDTGAITLRAIFPNPKTDLLPGMYVRAVLQEGVRQQGLLVPQQAV 314 Query: 391 VRDAGGSSV-MVVNAESKVEARPVKTGGARGNNWQVIDGLKEGDRVIVEGLQKVRPGAAV 449 RD G V VVNA+ VE R + T A G+ W V GLKEGD+++V+G Q+ GA V Sbjct: 315 SRDGAGKPVAFVVNAQGVVEQRALVTDRAIGDQWLVSSGLKEGDQLVVDGQQRASAGAKV 374 Query: 450 KPVQWNP 456 K V W P Sbjct: 375 KVVPWTP 381 >gi|300310851|ref|YP_003774943.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 162 bits (411), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 110/327 (33%), Positives = 170/327 (51%), Gaps = 21/327 (6%) Query: 142 AQVRARVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQTSLT 201 A VRARV G + K +KEG VK G +L +IDP +A+L A+ LAR +A L L Sbjct: 60 ATVRARVDGQLMKLHYKEGQVVKAGDLLAEIDPRSLQAALTQAEGQLARDRALLASARLD 119 Query: 202 AQRYKPLVEVNAVSKQEYDNAVAAQKQAEADVAAGKAAVQTANLNLGYATVTSPISGRIG 261 +RY+ L+ ++++ Q+ D VA KQ E V A + V +A L L + VT+PISGR+G Sbjct: 120 LKRYQTLLAQDSIASQQVDTQVALVKQYEGTVKADEGNVASARLQLSFTRVTAPISGRLG 179 Query: 262 RALVTEGALVGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQLKSVGDNKASV 321 G V D L ++ QL P+ + ++ K+ Q + +G+ Sbjct: 180 LRQADVGNNVTTSDTNGLVIITQLQPITAIFSIPEDNIPKVLQQLQSGRKLP-------- 231 Query: 322 TLVLENGQAYDQ-------PGKLLFSDISVDESTGSVSLRAEFPNPKRLLLPGTYVRTKI 374 QA+D+ G LL D VD +TG+V L+A+FPN L P +V ++ Sbjct: 232 ------AQAWDREQKNKLADGVLLTIDNVVDATTGTVKLKAQFPNTDYALFPSQFVNIRL 285 Query: 375 EQAVDEQALLVPQQAVVRDAGGSSVMVVNAESKVEARPVKTGGARGNNWQVIDGLKEGDR 434 + ++ A ++P A+ R + G V VV +S V RPVKTG + + + DG+ G+ Sbjct: 286 QLNTEQGATVIPTAAIQRGSKGLFVYVVKDDSSVTVRPVKTGPVQDDLTVITDGVSAGET 345 Query: 435 VIVEGLQKVRPGAAVKPVQWNPAGAPA 461 V+++G+ ++R GA V+ V A PA Sbjct: 346 VVIDGIDRLREGAKVEAVARGGADDPA 372 >gi|300309658|ref|YP_003773750.1| HlyD-family secretion protein [Herbaspirillum seropedicae SmR1] Length = 399 Score = 145 bits (367), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 114/338 (33%), Positives = 179/338 (52%), Gaps = 9/338 (2%) Query: 116 VSVVTIAPQRVAITNELPGRVEAFRVAQVRARVAGIVQKRVFKEGSEVKEGQVLFKIDPA 175 V V + +++ GR+EA +VR V G + FK+G+ VK+G VLF IDP Sbjct: 51 VDVAEVVSRQIIDWQSYSGRIEAVDRVEVRPLVGGTLTAVHFKDGALVKKGDVLFTIDPR 110 Query: 176 QYKASLESAQATLARAQANLTQTSLTAQRYKPLVEVNAVSKQEYDNAVAAQKQAEADVAA 235 Y+A + A+A LA A+A T+ R + L+ NA++K++++ A ++A A+VAA Sbjct: 111 PYEAEVARAEAQLAGAEARAGYTASDLARGQRLLSDNAIAKRDFEEKQNASREASANVAA 170 Query: 236 GKAAVQTANLNLGYATVTSPISGRIGRALVTEGALVGQGDATQ-LALVQQLDPVYVNVTQ 294 +AA++ A LNL Y +T+P+SGRI RA VT G +V G A L + + VY + Sbjct: 171 ARAALRAARLNLEYTRITAPVSGRISRAEVTVGNVVNPGAANAPLTTLVSVAKVYASFDV 230 Query: 295 SSADLLK-LQQAMAAGQLKSVGDNKASVTLVLENGQAYDQPGKLLFSDISVDESTGSVSL 353 LK + A AAG V L L N Y + G++ D +D ++G++ + Sbjct: 231 DEQSFLKYVNPARAAGA-------TVPVELGLANEDGYSRKGRVGSVDNRLDTASGTIRV 283 Query: 354 RAEFPNPKRLLLPGTYVRTKIEQAVDEQALLVPQQAVVRDAGGSSVMVVNAESKVEARPV 413 RA F N L+PG Y R ++ QA+L+ ++A+ D VMV + ++ R V Sbjct: 284 RAIFDNANSQLVPGLYARVRLGGGEPHQAVLIDEKAIGTDQDKRFVMVADKDNHANYRQV 343 Query: 414 KTGGARGNNWQVIDGLKEGDRVIVEGLQKVRPGAAVKP 451 G + GL+ G+R++V GLQ++RPG A+ P Sbjct: 344 VVGAGHDGLVVIEKGLQPGERIVVNGLQRIRPGDAITP 381 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.127 0.355 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,089,717 Number of extensions: 35955 Number of successful extensions: 195 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 185 Number of HSP's successfully gapped: 11 Length of query: 479 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 390 Effective length of database: 1,198,455 Effective search space: 467397450 Effective search space used: 467397450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 132 (55.5 bits)