BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0149.p (197 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309819|ref|YP_003773911.1| acriflavin resistance transmemb... 359 e-101 gi|300313244|ref|YP_003777336.1| multidrug efflux system transme... 222 1e-59 gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump pr... 187 4e-49 gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump pr... 182 1e-47 gi|300309657|ref|YP_003773749.1| cation/multidrug efflux pump pr... 114 4e-27 >gi|300309819|ref|YP_003773911.1| acriflavin resistance transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 359 bits (921), Expect = e-101, Method: Compositional matrix adjust. Identities = 177/180 (98%), Positives = 179/180 (99%) Query: 1 MGKFFIDRPVFAWVLAIFIMLGGALAITGLPVAQYPSIAPPTIVVTATYPGATAQTLDDS 60 MGKFFIDRPVFAWVLAIFIML GALAITGLPV+QYP+IAPPTIVVTATYPGATAQTLDDS Sbjct: 1 MGKFFIDRPVFAWVLAIFIMLAGALAITGLPVSQYPNIAPPTIVVTATYPGATAQTLDDS 60 Query: 61 VTSLIEQEMNGADGLQYIESQSQANGQVQITITFSPETNPDLAAVDVQNRLKRVEARLPA 120 VTSLIEQEMNGADGLQYIESQSQANGQVQITITFSPETNPDLAAVDVQNRLKRVEARLPA Sbjct: 61 VTSLIEQEMNGADGLQYIESQSQANGQVQITITFSPETNPDLAAVDVQNRLKRVEARLPA 120 Query: 121 AVTQQGVQVTKSRSNFLLFIGLSSTDGKLDPVALGDYLSRNVLNEIKRVPGVGQAQLFGT 180 AVTQQGVQVTKSRSNFLLFIGLSSTDGKLDPVALGDYLSRNVLNEIKRVPGVGQAQLFGT Sbjct: 121 AVTQQGVQVTKSRSNFLLFIGLSSTDGKLDPVALGDYLSRNVLNEIKRVPGVGQAQLFGT 180 >gi|300313244|ref|YP_003777336.1| multidrug efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1033 Score = 222 bits (566), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 100/179 (55%), Positives = 138/179 (77%) Query: 1 MGKFFIDRPVFAWVLAIFIMLGGALAITGLPVAQYPSIAPPTIVVTATYPGATAQTLDDS 60 M +FFIDRP+FAWV+A+FI+L G +A++ LP+A+YPS+APPT+ + ATYPGAT QT++D Sbjct: 1 MPRFFIDRPIFAWVVALFIVLFGVIAMSQLPIARYPSVAPPTVSINATYPGATPQTMNDG 60 Query: 61 VTSLIEQEMNGADGLQYIESQSQANGQVQITITFSPETNPDLAAVDVQNRLKRVEARLPA 120 V SLIE+E++G L Y ES + +G QI++TF P T+P+LA VDVQN+LK +EARLP Sbjct: 61 VISLIERELSGVKNLLYFESSADTSGTAQISVTFKPGTDPELAQVDVQNKLKAIEARLPQ 120 Query: 121 AVTQQGVQVTKSRSNFLLFIGLSSTDGKLDPVALGDYLSRNVLNEIKRVPGVGQAQLFG 179 V Q GV V + S+FLL + L+S DG+ D V L DYL+RN++ E++R+ GVG+ QLFG Sbjct: 121 TVRQNGVTVESAASSFLLMVSLTSVDGRYDEVRLNDYLARNIVEELRRMEGVGRVQLFG 179 >gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 187 bits (476), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 89/180 (49%), Positives = 129/180 (71%) Query: 1 MGKFFIDRPVFAWVLAIFIMLGGALAITGLPVAQYPSIAPPTIVVTATYPGATAQTLDDS 60 M +FFIDRP+FAWV+AI IMLGG ++I L + QYP IAPP + + ATY GA+A+TL+DS Sbjct: 1 MARFFIDRPIFAWVIAIIIMLGGMMSIRSLSLEQYPDIAPPKVNIKATYTGASAKTLEDS 60 Query: 61 VTSLIEQEMNGADGLQYIESQSQANGQVQITITFSPETNPDLAAVDVQNRLKRVEARLPA 120 VT +IEQ+M G D LQY+ + S + G +++TF+ TNPD+A + VQN+L++V ARLP Sbjct: 61 VTQVIEQQMKGLDNLQYMSASSSSAGSATVSLTFTAGTNPDVAQMQVQNKLQQVTARLPQ 120 Query: 121 AVTQQGVQVTKSRSNFLLFIGLSSTDGKLDPVALGDYLSRNVLNEIKRVPGVGQAQLFGT 180 AV GV VTK+ ++ L+ + L+S D ++ + +GD++S + ++I RV GVG FGT Sbjct: 121 AVQNNGVTVTKASTDILMVLSLTSDDPRITSIDIGDFISSTLTDQISRVEGVGDVTAFGT 180 >gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1049 Score = 182 bits (463), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 96/180 (53%), Positives = 127/180 (70%) Query: 1 MGKFFIDRPVFAWVLAIFIMLGGALAITGLPVAQYPSIAPPTIVVTATYPGATAQTLDDS 60 M +FFIDRP+FAWVLAI +ML G +++ LP+AQYP+IAPP I +TA YPGA+A+TL+D+ Sbjct: 1 MARFFIDRPIFAWVLAIIVMLAGIVSVLTLPIAQYPAIAPPAIAITANYPGASAKTLEDT 60 Query: 61 VTSLIEQEMNGADGLQYIESQSQANGQVQITITFSPETNPDLAAVDVQNRLKRVEARLPA 120 VT +IEQ+M G D L Y+ S S+++G V IT+TF TNPD A V VQN+L LP+ Sbjct: 61 VTQIIEQKMKGLDRLSYMASTSESSGSVTITLTFENGTNPDTAQVQVQNKLSLATPLLPS 120 Query: 121 AVTQQGVQVTKSRSNFLLFIGLSSTDGKLDPVALGDYLSRNVLNEIKRVPGVGQAQLFGT 180 V QQG+ VTKS +NFL + +S DG + L DY++ NV + I RV GVG LFG+ Sbjct: 121 EVQQQGITVTKSATNFLNVLAFTSEDGSMSGSDLSDYVAANVQDAISRVEGVGDTTLFGS 180 >gi|300309657|ref|YP_003773749.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1071 Score = 114 bits (286), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 71/180 (39%), Positives = 115/180 (63%) Query: 1 MGKFFIDRPVFAWVLAIFIMLGGALAITGLPVAQYPSIAPPTIVVTATYPGATAQTLDDS 60 + KFFIDRP+FA VL++ ++LGG LA+ LP+++YP + PP++VV A YPGA + + ++ Sbjct: 3 ISKFFIDRPIFAGVLSVLLLLGGVLAMLQLPISEYPEVVPPSVVVRAQYPGANPKVIAET 62 Query: 61 VTSLIEQEMNGADGLQYIESQSQANGQVQITITFSPETNPDLAAVDVQNRLKRVEARLPA 120 V S +E+++NG + + Y++SQ+ ++G + IT+ F +PD A VQNR+ + RLP Sbjct: 63 VASPLEEQINGVENMLYMQSQANSDGNLTITVNFRLGVDPDKAQQLVQNRVSQALPRLPE 122 Query: 121 AVTQQGVQVTKSRSNFLLFIGLSSTDGKLDPVALGDYLSRNVLNEIKRVPGVGQAQLFGT 180 V + GV KS + + L+S D + D L +Y NV + + R+ GVG+ LFG+ Sbjct: 123 DVQRLGVTTLKSSPTLTMVVHLTSPDNRYDTTYLRNYAVLNVKDRLARIQGVGEVGLFGS 182 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 620,366 Number of extensions: 23711 Number of successful extensions: 105 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 103 Number of HSP's successfully gapped: 8 Length of query: 197 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 117 Effective length of database: 1,241,070 Effective search space: 145205190 Effective search space used: 145205190 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)