BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0146.p (483 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 912 0.0 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 436 e-123 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 406 e-114 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 382 e-107 gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipo... 241 8e-65 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 912 bits (2357), Expect = 0.0, Method: Compositional matrix adjust. Identities = 453/483 (93%), Positives = 479/483 (99%) Query: 1 MANLSLPRSPRLLQLCAALMVAGVLGGCSLAPTYDRPQAPVETAYPADAAGRAELQAVNL 60 MANLS+PRSPRLL +CAAL++AGVLGGCSLAPTY+RPQAPVE++YPADAAGRAELQAVNL Sbjct: 1 MANLSMPRSPRLLHMCAALVMAGVLGGCSLAPTYERPQAPVESSYPADAAGRAELQAVNL 60 Query: 61 GWRQFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNVNGSAARTR 120 GWRQFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLN++G+AARTR Sbjct: 61 GWRQFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAARTR 120 Query: 121 TAASQSLTGQPFISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLI 180 TAASQSLTGQP ISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISL+ Sbjct: 121 TAASQSLTGQPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLV 180 Query: 181 AQVAQAYLSERSYAEQLQLANDTLKSRESNLDLAQKRFDVGASSALDFRLTQTLVESARV 240 AQVAQAYL+ERSYAEQL+LA++TLKSR+SNLDLAQKR DVGAS+ALDFRLTQTLVESARV Sbjct: 181 AQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESARV 240 Query: 241 SKSQLERQRAQAENALTLLVGKKVGDLPPAQLLSDEKIVTDIPAGLPSDLLTNRPDIRSA 300 +K+QLERQRAQAENALTLLVGKK+G+LPPAQLLSDEKIVTDIPAGLPSDLLTNRPDIRSA Sbjct: 241 AKTQLERQRAQAENALTLLVGKKIGELPPAQLLSDEKIVTDIPAGLPSDLLTNRPDIRSA 300 Query: 301 EQSLLAANANIGAARAAFFPRISLTGSYGSASSTLGGLFESGSGAWSFGPSLSLPIFDFG 360 EQSLLAANANIGAARAAFFPRISLTG++GSASSTLGGLFESGSGAWSFGPSLSLPIFDFG Sbjct: 301 EQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFDFG 360 Query: 361 RNSANLDLAEVRKNKAVVTYEKTIQTAFREVADALVARGTLDDQVKAQEAFLAAQQERLK 420 RNSANLDLAEVRKNKAVVTYEK IQTAFREVADALVARG+LD+QVKAQEAFLAAQQERLK Sbjct: 361 RNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAAQQERLK 420 Query: 421 LTELRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLNENTVSVA 480 LT+LRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLNENTVSVA Sbjct: 421 LTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLNENTVSVA 480 Query: 481 AKQ 483 AKQ Sbjct: 481 AKQ 483 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 436 bits (1121), Expect = e-123, Method: Compositional matrix adjust. Identities = 252/487 (51%), Positives = 319/487 (65%), Gaps = 17/487 (3%) Query: 8 RSPRLLQLCAALMVAGVLGGCSLAPTYDRPQAPVETAYPA----DAAGRAELQAV-NLGW 62 RSP +L + +M+A +LGGC+L P Y RP APV + +AAG + V ++GW Sbjct: 8 RSP-VLPMMLPVMLAMLLGGCTLMPDYHRPDAPVAAHFAGADSKNAAGASSAPVVADIGW 66 Query: 63 RQFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNVNGSAARTRTA 122 R F D LQQ+IA ALENNRDLR AVLNIE+ARAQY + RA L P++ S +RT Sbjct: 67 RDVFTDPSLQQVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAATSETASRTP 126 Query: 123 ASQSLTGQPFISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLIAQ 182 S TG S SY L AYELD FGRVR+LK+ AL S+LST EA++S QISLIA+ Sbjct: 127 GDLSSTGSALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQISLIAE 186 Query: 183 VAQAYLSERSYAEQLQLANDTLKSRESNLDLAQKRFDVGASSALDFRLTQTLVESARVSK 242 VA A+L+ S ++L+LA DTL+S+ S+ L Q+ F++G+SSAL R QT VESARV Sbjct: 187 VATAWLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTSVESARVDV 246 Query: 243 SQLERQRAQAENALTLLVGKKVGDLPPAQL---LSDEKI-----VTDIPAGLPSDLLTNR 294 Q Q NAL LLVGK DLP A L L D ++ + IP GLPSDLL R Sbjct: 247 ESYTAQVDQDRNALVLLVGK---DLPTALLPQGLPDTRLAAASPLATIPPGLPSDLLQRR 303 Query: 295 PDIRSAEQSLLAANANIGAARAAFFPRISLTGSYGSASSTLGGLFESGSGAWSFGPSLSL 354 DI AE+ L AANANIGAARAAF+P I+LT + G+AS+ L GLF+ GSG+WSF P +SL Sbjct: 304 ADIVQAERDLRAANANIGAARAAFYPSITLTVNAGTASARLAGLFKGGSGSWSFVPQISL 363 Query: 355 PIFDFGRNSANLDLAEVRKNKAVVTYEKTIQTAFREVADALVARGTLDDQVKAQEAFLAA 414 PIFD G N ANLD+A V ++ +V YEK IQTAFREV+DAL R TL Q++AQEA + A Sbjct: 364 PIFDGGANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQEALVQA 423 Query: 415 QQERLKLTELRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLNE 474 E +L++ R+ +G+ S L LD+QR L+SAQ+ LI + R N + YK LGGG E Sbjct: 424 SDEAFRLSQARYHSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYKTLGGGWVE 483 Query: 475 NTVSVAA 481 T + AA Sbjct: 484 RTGTPAA 490 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 406 bits (1044), Expect = e-114, Method: Compositional matrix adjust. Identities = 225/462 (48%), Positives = 303/462 (65%), Gaps = 12/462 (2%) Query: 27 GCS-LAPTYDRPQAPVETAYPADAAGR----------AELQAVNLGWRQFFPDQRLQQLI 75 GC+ LAP Y +P APV ++ + AA + E+ A +LGWR+FF D RLQQ+I Sbjct: 27 GCANLAPDYTQPAAPVPASWTSQAAAQPAVGSAGPAAGEIDADHLGWREFFLDPRLQQVI 86 Query: 76 AAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNVNGSAARTRTAASQSLTGQPFISN 135 AAAL+NNRDLR A LNIE+ARAQY+I A+ P+++ G+++ +RT A S TG +S+ Sbjct: 87 AAALDNNRDLRVAALNIEKARAQYRITDANRYPSISATGTSSASRTPAELSTTGSAAVSH 146 Query: 136 SYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLIAQVAQAYLSERSYAE 195 V + V AYELDFFGR++NL DAAL SY E ++S ISL+A+VA A+L+ S + Sbjct: 147 QQSVTVGVSAYELDFFGRLKNLSDAALESYQYQVETRRSTHISLVAEVATAWLTLASDRD 206 Query: 196 QLQLANDTLKSRESNLDLAQKRFDVGASSALDFRLTQTLVESARVSKSQLERQRAQAENA 255 L+LA DT S+ L+Q+ D+G S +D + VESAR ++ Q Q NA Sbjct: 207 LLKLAQDTYASQSRTFALSQRSHDIGTVSGVDLASQEATVESARADVARYASQVEQDLNA 266 Query: 256 LTLLVGKKV-GDLPPAQLLSDEKIVTDIPAGLPSDLLTNRPDIRSAEQSLLAANANIGAA 314 L LLVG + L PA L ++ PAGLPS +L RPD+ +AE +L +ANA+IGAA Sbjct: 267 LRLLVGADIDAALLPAGLPDTASVLPATPAGLPSQVLLRRPDVLAAEHTLKSANADIGAA 326 Query: 315 RAAFFPRISLTGSYGSASSTLGGLFESGSGAWSFGPSLSLPIFDFGRNSANLDLAEVRKN 374 RAAFFP ISLT + G+AS TLGGLF++GSG+W+F PS++LPIF+ G A+LD A++ ++ Sbjct: 327 RAAFFPSISLTANGGTASRTLGGLFKAGSGSWTFSPSINLPIFNAGSLRASLDSAKITRD 386 Query: 375 KAVVTYEKTIQTAFREVADALVARGTLDDQVKAQEAFLAAQQERLKLTELRFKNGIASSL 434 V YEK IQTAFREVADAL R TLD ++ AQ AA ++ +L++ R++NG+ S L Sbjct: 387 IDVANYEKAIQTAFREVADALAVRATLDQRLDAQGKATAASEKAFRLSDARYRNGVDSYL 446 Query: 435 DQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLNENT 476 L AQR L+SAQQ LI R +N I LYK LGGG +E T Sbjct: 447 TTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVLGGGWSEQT 488 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 382 bits (981), Expect = e-107, Method: Compositional matrix adjust. Identities = 220/451 (48%), Positives = 297/451 (65%), Gaps = 9/451 (1%) Query: 28 C-SLAPTYDRPQAPVETAYPADAAGRAELQAVN------LGWRQFFPDQRLQQLIAAALE 80 C SLAP Y RP APV YP A Q N L WR +F D LQ LI ALE Sbjct: 25 CQSLAPHYQRPAAPVAAVYPDGPVNEAAHQVANEAASPPLPWRDYFTDPPLQALITQALE 84 Query: 81 NNRDLRTAVLNIEEARAQYQIARADLLPNLNVNGSAARTRTAASQSLTGQPFISNSYQVG 140 N+RDLRTA L ++EA+A Y I ADL P + R+RT A +LT +P + ++YQ G Sbjct: 85 NSRDLRTAALRVQEAQALYGIGHADLFPTIGAQAGLDRSRTPADLNLTRRPLVGSAYQAG 144 Query: 141 LAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLIAQVAQAYLSERSYAEQLQLA 200 L + ++E+D +GR+R+L DAAL S +++ + + +SL+AQVA A+L E+L++A Sbjct: 145 LGLSSWEIDLWGRLRSLDDAALESARASQATRSAVALSLMAQVADAWLELAELDERLEIA 204 Query: 201 NDTLKSRESNLDLAQKRFDVGASSALDFRLTQTLVESARVSKSQLERQRAQAENALTLLV 260 N T+ SR+ + + +R +VGA+S L+ +TL+ A+ +QL++QRAQ NALTLLV Sbjct: 205 NQTIASRQESFRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNALTLLV 264 Query: 261 GKKVGDLPPAQLLSD-EKIVTDIPAGLPSDLLTNRPDIRSAEQSLLAANANIGAARAAFF 319 G V LP Q + + + + GLP+ LLT RPD+ +AE L+AANANIGAARAAFF Sbjct: 265 GSPV-VLPVGQARAGLDAVFAPLQPGLPALLLTRRPDVAAAEHRLMAANANIGAARAAFF 323 Query: 320 PRISLTGSYGSASSTLGGLFESGSGAWSFGPSLSLPIFDFGRNSANLDLAEVRKNKAVVT 379 P ISLTG+ G+AS+ L GLF GS AW+F PS++LPIF GR NL L+EVR++ AV Sbjct: 324 PSISLTGNVGTASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVRRDLAVAA 383 Query: 380 YEKTIQTAFREVADALVARGTLDDQVKAQEAFLAAQQERLKLTELRFKNGIASSLDQLDA 439 YEKT+QTAFREVADAL AR L Q++ ++ + Q+ER +L+ LR+ NG A LD LDA Sbjct: 384 YEKTVQTAFREVADALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAPFLDVLDA 443 Query: 440 QRDLFSAQQALIQARQLRLNNAIDLYKALGG 470 QRDL SAQQ L+Q R+ L++++ LY ALGG Sbjct: 444 QRDLLSAQQQLVQIRRALLSSSVALYAALGG 474 >gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 481 Score = 241 bits (616), Expect = 8e-65, Method: Compositional matrix adjust. Identities = 172/469 (36%), Positives = 256/469 (54%), Gaps = 11/469 (2%) Query: 24 VLGGCSLAP-TYDRPQAPVE--TAYP----ADAAGRAELQAVNLGWRQFFPDQRLQQLIA 76 L C+ AP T P + ++ T YP DA A A +GW+ +FP LQ LIA Sbjct: 14 TLALCACAPLTAPLPPSALDLPTHYPRTLAEDAPANAAPTASAIGWQDYFPQAPLQALIA 73 Query: 77 AALENNRDLRTAVLNIEEARAQYQIARADLLPNLNVNGSAARTRTAASQSLTGQPFISNS 136 AL +N DLR L +++ +AQ++I R+ LP +N+ A R+R +G + Sbjct: 74 EALRHNPDLRDIRLRLQQVQAQWRIQRSTGLPTVNLEAQARRSRGPLEPPESGPAASTRE 133 Query: 137 YQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLIAQVAQAYLSERSYAEQ 196 Y+VGL ++ELDF+GR+ +L +AAL ++++ A+++ ISLI QVA A+ S R E+ Sbjct: 134 YRVGLGASSWELDFWGRIASLNEAALQEWMASRAAEQALVISLIGQVAAAWTSLRELDER 193 Query: 197 LQLANDTLKSRESNLDLAQKRFDVGASSALDFRLTQTLVESARVSKSQLERQRAQAENAL 256 L L + +L L Q R D G + A ++ + R +++L++ RA + L Sbjct: 194 LHLLETLQAATAESLALMQARHDAGRADAAALAQQRSELAQMRAEQAELQQMRASQRSTL 253 Query: 257 TLLVGKKVGDLPPAQLLSDEKIVTD--IPAGLPSDLLTNRPDIRSAEQSLLAANANIGAA 314 +L G + LP A + I+ + GLPS LL RPDI +AE L A+ A + AA Sbjct: 254 EMLAG--LTQLPAALEPAPTTILLPFALAPGLPSTLLAARPDIIAAEHRLQASAAQVAAA 311 Query: 315 RAAFFPRISLTGSYGSASSTLGGLFESGSGAWSFGPSLSLPIFDFGRNSANLDLAEVRKN 374 RAAFFPRISLT G AS+ L LF+ S AW+F P +SLP+ D G + D+A + + Sbjct: 312 RAAFFPRISLTAFGGVASTALQDLFKGPSRAWNFVPEISLPLLDGGLRQSRYDIAALDQQ 371 Query: 375 KAVVTYEKTIQTAFREVADALVARGTLDDQVKAQEAFLAAQQERLKLTELRFKNGIASSL 434 +A++ YE+T+Q AFREVA L L +Q++ + + Q +R +R + G AS L Sbjct: 372 RALLDYERTVQNAFREVAQRLEEAHWLKEQLRLRREVVQQQAQRQAQMHMRLRYGRASRL 431 Query: 435 DQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLNENTVSVAAKQ 483 D L+++RD AQQ L N I LY ALGGG + S ++Q Sbjct: 432 DVLESERDHLRAQQRQHMLEHALLRNQIALYVALGGGTQALSASSPSQQ 480 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.129 0.348 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,205,193 Number of extensions: 44131 Number of successful extensions: 304 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 248 Number of HSP's successfully gapped: 18 Length of query: 483 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 394 Effective length of database: 1,198,455 Effective search space: 472191270 Effective search space used: 472191270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)