BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0002.0144.p
         (337 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309817|ref|YP_003773909.1| UDP-glucose 4-epimerase [Herbas...   413   e-117
gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydra...    60   2e-10
gi|300313890|ref|YP_003777982.1| nucleoside-diphosphate-sugar ep...    55   6e-09

>gi|300309817|ref|YP_003773909.1| UDP-glucose 4-epimerase
           [Herbaspirillum seropedicae SmR1]
          Length = 320

 Score =  413 bits (1062), Expect = e-117,   Method: Compositional matrix adjust.
 Identities = 206/316 (65%), Positives = 242/316 (76%)

Query: 14  MRILVTGASGFVGSALLRHLAPRPDLALRAALRTAASGGLLPAGVEVVETGDLGPQTNWH 73
           M ILVTGASGFVG ALL  L       LR A R ++   +    +E      L P+T+W 
Sbjct: 1   MHILVTGASGFVGRALLSRLDSEGQHLLRGAFRPSSETVMDRERIERTVISGLDPETDWS 60

Query: 74  AALTGIDTVVHCAARVHVMNETAGDPLSLFRRVNVEGSLALAQQARDAGVKRLVFLSSIK 133
           AAL  I+ VVHCAARVHVMNETAGDPL+ FRR+N+EGSLALA+QA+ AGV+R +FLSSIK
Sbjct: 61  AALHNIEAVVHCAARVHVMNETAGDPLAEFRRINLEGSLALARQAQQAGVQRFIFLSSIK 120

Query: 134 VNGEQTAPGRPFTADDTPAPVDAYGISKMEAEQALMALAAEGGMEVVVIRPVLVYGPGVK 193
           VNGEQT+ GRPFTADD PAP D YGISKMEAEQAL+ L   G M+VV+IRPVLVYGPGVK
Sbjct: 121 VNGEQTSLGRPFTADDKPAPTDPYGISKMEAEQALLELGRTGKMQVVIIRPVLVYGPGVK 180

Query: 194 ANFRSMMRWLQKGIPLPLGAITGNRRSLVALDNLVDLMTLCMHHPAAANEIFLVSDGEDL 253
           ANF SMMRWL KG+PLPLG IT NRRSLVALDNLVDL+T+C+ HPAAA ++FL SDGEDL
Sbjct: 181 ANFLSMMRWLDKGMPLPLGGITHNRRSLVALDNLVDLITVCLAHPAAAGQVFLASDGEDL 240

Query: 254 STTELLRRMGQALGSPARLIPVPARVLEWGARLLGKGAVAQRLCGSLQVEIGKTRSVLDW 313
           STT +L+R+G+AL     L+PVPA  L   ARL+GK  +AQRLCGSLQV+I K R +L W
Sbjct: 241 STTGMLQRLGRALDKSPTLLPVPASWLTLAARLMGKKGIAQRLCGSLQVDISKNRELLGW 300

Query: 314 SPPVSTDVALRRAASA 329
           SPP++ D A R+   A
Sbjct: 301 SPPITVDAAFRKTVDA 316


>gi|300313483|ref|YP_003777575.1| NAD_dependent
           epimerase/dehydratase [Herbaspirillum seropedicae SmR1]
          Length = 333

 Score = 60.5 bits (145), Expect = 2e-10,   Method: Compositional matrix adjust.
 Identities = 57/188 (30%), Positives = 82/188 (43%), Gaps = 22/188 (11%)

Query: 15  RILVTGASGFVGSALLRHLAPRP-------DLALRAALRTAASGGLLPAGVEVVETGDLG 67
           +I+V G +G +GS  + HL           D  LR + R      L    V++ + G   
Sbjct: 7   KIVVIGGAGLIGSHTVDHLVKEDVKEILIYDNFLRGS-RENLQNALKDPRVKIYDVGGDI 65

Query: 68  PQTN-WHAALTGIDTVVHCAA----RVHVMNETAGDPLSLFRRVNVEGSLALAQQARDAG 122
            QT+   +AL G D V H AA    + H    +A D       VNV G+  + +     G
Sbjct: 66  MQTDILQSALEGADGVFHLAALWLLQCHEYPRSAFD-------VNVRGTFNVMEACVAKG 118

Query: 123 VKRLVFLSSIKVNGEQTAPGRPFTADDTPAPVDAYGISKMEAEQALMALAAEGGMEVVVI 182
           VKRLV+ SS  V G+  A   P   D      + YG +K+  E  L +     G+  V +
Sbjct: 119 VKRLVYSSSASVYGD--AVREPMDEDHPYNNKNFYGATKIAGETMLRSFHHRYGLNYVGL 176

Query: 183 RPVLVYGP 190
           R + VYGP
Sbjct: 177 RYMNVYGP 184


>gi|300313890|ref|YP_003777982.1| nucleoside-diphosphate-sugar
           epimerase [Herbaspirillum seropedicae SmR1]
          Length = 284

 Score = 55.5 bits (132), Expect = 6e-09,   Method: Compositional matrix adjust.
 Identities = 55/191 (28%), Positives = 79/191 (41%), Gaps = 9/191 (4%)

Query: 13  FMRILVTGASGFVGSALLRHLAPRPDLALRAALRTAASGGLLPAGVEVVETGDLGPQTNW 72
           F R+L+TGA+G VG  L   LA   ++   A L +A +     A  E     DL  +   
Sbjct: 15  FSRLLLTGAAGGVGKQLRARLASFAEVVRVADLASAMAAVDPAAAHEEALGCDLADRAAV 74

Query: 73  HAALTGIDTVVHCAARVHVMNETAGDPLSLFRRVNVEGSLALAQQARDAGVKRLVFLSSI 132
            A + G      C A +H+   +   P       N++G   + + AR  GVKR++F SS 
Sbjct: 75  DAMVAG------CEAIIHLGGVSVERPFEEILEANIKGVFHIYEAARRHGVKRVIFASSN 128

Query: 133 KVNGEQTAPGRPFTADDTPAPVDAYGISKMEAEQALMALAAEGGMEVVVIRPVLVYGPGV 192
            V G      R   A D   P   YG+SK   E          G+E V IR   ++    
Sbjct: 129 HVTGFYGQDER-IDARDMKRPDGYYGLSKSYGEDMAQFYFDRYGVETVSIRIGSIFPEAT 187

Query: 193 KANFRSMMRWL 203
             N R +  W+
Sbjct: 188 --NRRMLASWM 196


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.320    0.135    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 962,031
Number of extensions: 37934
Number of successful extensions: 162
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 167
Number of HSP's successfully gapped: 3
Length of query: 337
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 251
Effective length of database: 1,212,660
Effective search space: 304377660
Effective search space used: 304377660
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 131 (55.1 bits)