BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0094.p (267 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309803|ref|YP_003773895.1| nitrate/sulfonate/bicarbonate A... 483 e-138 gi|300313548|ref|YP_003777640.1| nitrate/sulfonate/bicarbonate A... 116 2e-27 gi|300312968|ref|YP_003777060.1| nitrate/sulfonate/bicarbonate A... 81 8e-17 gi|300312684|ref|YP_003776776.1| nitrate/sulfonate/bicarbonate A... 75 4e-15 gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate A... 73 3e-14 >gi|300309803|ref|YP_003773895.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 267 Score = 483 bits (1244), Expect = e-138, Method: Compositional matrix adjust. Identities = 257/267 (96%), Positives = 260/267 (97%) Query: 1 MKPNTDPLVNRPGFWRVVAPLSIGIALLLMWQIVCTLLEVPPYLVPTPALIAKTLVADWG 60 MKPNTDPLVNRPGFWRV APL IGIALLLMWQIVCTLLEVPPYLVPTPALI KTL ADW Sbjct: 1 MKPNTDPLVNRPGFWRVAAPLIIGIALLLMWQIVCTLLEVPPYLVPTPALIVKTLAADWS 60 Query: 61 MLMESLLVTLKITFLAFVLAVILGVSIAFVFVQSRFIEVSLFPYAIILQVTPIVAIAPLI 120 MLM SLLVTLKITFLAF LAV+LGV+IAFVFVQSRFIE+SLFPYAIILQVTPIVAIAPLI Sbjct: 61 MLMASLLVTLKITFLAFALAVVLGVAIAFVFVQSRFIEISLFPYAIILQVTPIVAIAPLI 120 Query: 121 IIWVKDTTAALTVCATIMALFPIISNTTLGLRSVNPGLLNLFRLNKATRWQTLRRLRIPS 180 IIWVKDTTAALTVCATIMALFPIISNTTLGLRSVNPGLLNLFRLNKATRWQTLRRLRIPS Sbjct: 121 IIWVKDTTAALTVCATIMALFPIISNTTLGLRSVNPGLLNLFRLNKATRWQTLRRLRIPS 180 Query: 181 ALPYFFGGLRISSGLALIGAVVAEFVAGTGGTGSGLAYQILQAGFQLNIPRLFAALLLIT 240 ALPYFFGGLRISSGLALIGAVVAEFVAGTGGTGSGLAYQILQAGFQLNIPRLFAALLLIT Sbjct: 181 ALPYFFGGLRISSGLALIGAVVAEFVAGTGGTGSGLAYQILQAGFQLNIPRLFAALLLIT 240 Query: 241 LTGILLFWLMSALSRAMLAGWHESEMA 267 LTGILLFWLMSALSRAMLAGWHESEMA Sbjct: 241 LTGILLFWLMSALSRAMLAGWHESEMA 267 >gi|300313548|ref|YP_003777640.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 259 Score = 116 bits (290), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 67/248 (27%), Positives = 131/248 (52%), Gaps = 6/248 (2%) Query: 19 APLSIGIALLLMWQIVCTLLEVPPYLVPTPALIAKTLVADWGMLMESLLVTLKITFLAFV 78 AP + + +L +WQ +C++ +V ++ P+P I + +V G + E L T +T + F Sbjct: 17 APWFLLLVILGVWQFICSVWQVSDFIFPSPGSILQAMVDYAGPITEHALSTFWVTMVGFA 76 Query: 79 LAVILGVSIAFVFVQSRFIEVSLFPYAIILQVTPIVAIAPLIIIWVKDTTAALTVCATIM 138 +AV++GV++ FV S + + +P P A P++++W + A ++ Sbjct: 77 IAVVVGVTLGFVIGSSPMLYAAAYPLMTAFNALPKAAFVPVLVVWFGIGAGPAILTAFLI 136 Query: 139 ALFPIISNTTLGLRSVNPGLLNLFRLNKATRWQTLRRLRIPSALPYFFGGLRISSGLALI 198 + FPI+ N GL ++ P L ++ R+ A R L ++ +P ++PYFF L+++ LA + Sbjct: 137 SFFPIMVNIATGLATLEPELEDVLRVLGARRMDILLKVGLPRSMPYFFASLKVAITLAFV 196 Query: 199 GAVVAEFVAGTGGTGSGLAYQILQAGFQLNIPRLFAALLLITLTGILLFWLMSALSRAML 258 G+ V+E A + G+ Y ++ AG + +P FA L++I + ++ L + + + Sbjct: 197 GSTVSEMNA----SNQGIGYLLVSAGSSMKMPLAFAGLVVIGAMAMAMYELFAIIEKRT- 251 Query: 259 AGW-HESE 265 GW H + Sbjct: 252 TGWAHRGK 259 >gi|300312968|ref|YP_003777060.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 283 Score = 81.3 bits (199), Expect = 8e-17, Method: Compositional matrix adjust. Identities = 58/185 (31%), Positives = 92/185 (49%), Gaps = 4/185 (2%) Query: 62 LMESLLVTLKITFLAFVLAVILGVSIAFVFVQSRFIEVSLFPYAIILQVTPIVAIAPLII 121 + LL+TL T LAFV+ + G++I S L PY P V +AP+ Sbjct: 79 IYSHLLITLTETVLAFVIGTVFGLAIGLWLALSPTASAILDPYVKAANSMPRVILAPIFA 138 Query: 122 IWVKDTTAALTVCATIMALFPIISNTTLGLRSVNPGLLNLFRLNKATRWQTLRRLRIPSA 181 +W + A + F + N G++ V+P +L R+ A Q LR + +PSA Sbjct: 139 MWFGLGIWSKVALAVTLVFFIVFFNVYQGVKEVSPVVLANARMLGANARQLLRTVYLPSA 198 Query: 182 LPYFFGGLRISSGLALIGAVVAEFVAGTGGTGSGLAYQILQAGFQLNIPRLFAALLLITL 241 + F L S GLA +GAVV E++ G+ G+ Y ILQA +I +FA ++++T+ Sbjct: 199 TSWVFSSLHTSIGLAFVGAVVGEYL----GSARGVGYLILQAEGTFDINTVFAGIVVLTI 254 Query: 242 TGILL 246 ++L Sbjct: 255 FALVL 259 >gi|300312684|ref|YP_003776776.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 284 Score = 75.5 bits (184), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 64/235 (27%), Positives = 110/235 (46%), Gaps = 12/235 (5%) Query: 31 WQIVCTLLEVPPYLVPTPALIAKTLVADW-------GMLMESLLVTLKITFLAFVLAVIL 83 W++ + + P+ P+LI + DW G L +LVTL+ T L F++ + Sbjct: 48 WEVCARIGWIDPFFFSQPSLIVAQIY-DWMVEGTSQGPLWTQVLVTLEETVLGFLIGSVA 106 Query: 84 GVSIAFVFVQSRFIEVSLFPYAIILQVTPIVAIAPLIIIWVKDTTAALTVCATIMALFPI 143 GV V +++ + Y I P V + + +I A+ A +M F + Sbjct: 107 GVIAGIVLGRNKLLSDVFSLYIQIANSIPRVVLGSIFVIAFGLGMASKVALAVVMVFFVV 166 Query: 144 ISNTTLGLRSVNPGLLNLFRLNKATRWQTLRRLRIPSALPYFFGGLRISSGLALIGAVVA 203 +N G+R + ++ ++ A+R Q + IPSAL + L +S G AL+GAVV Sbjct: 167 FANAFQGVREADRYMIANAQILGASRRQVTMAVVIPSALSWILASLHVSFGFALVGAVVG 226 Query: 204 EFVAGTGGTGSGLAYQILQAGFQLNIPRLFAALLLITLTGILLFWLMSALSRAML 258 EF+ G+ G+ I A N +FAA++++ + + WL+ AL R +L Sbjct: 227 EFL----GSKQGIGLLISTAQGAFNASGVFAAMIVLAVVALAADWLLHALERRLL 277 >gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 278 Score = 72.8 bits (177), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 64/214 (29%), Positives = 100/214 (46%), Gaps = 9/214 (4%) Query: 3 PNTDPLVNRPG-FWRVVAPLSIGIALLLMWQIVCTLLEVPPYLVPTPALIAKTL--VADW 59 P+ D RPG F + PL A LL+ + L +P L+P P+ +A TL +A Sbjct: 16 PHDD--AYRPGRFTGWIVPL----AFLLLVEAGARLELIPARLLPPPSELAHTLWSLAQR 69 Query: 60 GMLMESLLVTLKITFLAFVLAVILGVSIAFVFVQSRFIEVSLFPYAIILQVTPIVAIAPL 119 G L+ + +L FV+ L V++ + SR E L P L+ P +A PL Sbjct: 70 GELLPHIAASLARVAAGFVIGSALAVALGLLVGISRRAEALLEPSFQALRAIPSLAWIPL 129 Query: 120 IIIWVKDTTAALTVCATIMALFPIISNTTLGLRSVNPGLLNLFRLNKATRWQTLRRLRIP 179 +++W+ V I A FP+ N G+R ++ L+ + + + + +P Sbjct: 130 LLLWLGIDETPKVVLIAIGAFFPVYLNLVAGIRDIDHKLIEVGTTSGLNTQALIGHILLP 189 Query: 180 SALPYFFGGLRISSGLALIGAVVAEFVAGTGGTG 213 ++LPY F GLR LA + V AE +A T G G Sbjct: 190 ASLPYLFAGLRGGLSLAWMFLVAAELIAATKGVG 223 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.142 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 788,795 Number of extensions: 31488 Number of successful extensions: 221 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 220 Number of HSP's successfully gapped: 14 Length of query: 267 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 183 Effective length of database: 1,222,130 Effective search space: 223649790 Effective search space used: 223649790 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)