BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0074.p (490 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) ... 900 0.0 gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirill... 499 e-142 gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum s... 149 6e-37 gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum... 125 6e-30 gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herb... 86 5e-18 >gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) kinase [Herbaspirillum seropedicae SmR1] Length = 490 Score = 900 bits (2326), Expect = 0.0, Method: Compositional matrix adjust. Identities = 461/488 (94%), Positives = 470/488 (96%) Query: 1 MYLGIDLGTSEVKAVVIDETGALLALAGSTLTVSRPHPRWSEQSPADWWLATCATVDKLR 60 MYLGIDLGTSEVKAVVID G+L+ALAGSTL V+RPHPRWSEQ+PADWW ATC TV KLR Sbjct: 1 MYLGIDLGTSEVKAVVIDAQGSLVALAGSTLNVARPHPRWSEQAPADWWQATCDTVAKLR 60 Query: 61 EQLGSERFGRIRAIGLSGQMHGAVLMDAQDAVLRPAILWSDSRSAPECAELERRAPRLHG 120 QLGSERFG IRAIGLSGQMHGAVL+D +D VLRPAILWSDSRSAPECAELE RAPRLHG Sbjct: 61 TQLGSERFGSIRAIGLSGQMHGAVLLDERDEVLRPAILWSDSRSAPECAELESRAPRLHG 120 Query: 121 IAGNLAMPGFTAPKLLWVARHEPQLFARIATVLLPKDWLRFMMTGRKVCDPSDAAGTLWL 180 IAGNLAMPGFTAPKLLWVARHEPQLFARIATVLLPKDWLR MTGRKV DPSDAAGTLWL Sbjct: 121 IAGNLAMPGFTAPKLLWVARHEPQLFARIATVLLPKDWLRLKMTGRKVSDPSDAAGTLWL 180 Query: 181 DVEGRNWSDELLAASGMRRDQMPALVDGSAVSGTLLPEVAQAWGLRADVIVAGGAGDGAA 240 DVEGRNWSDELLAASGMRRDQMPALVDGSAVSG+LLPEVAQAWGLR+DVIVAGGAGDGAA Sbjct: 181 DVEGRNWSDELLAASGMRRDQMPALVDGSAVSGSLLPEVAQAWGLRSDVIVAGGAGDGAA 240 Query: 241 SAVGIGAVKPGDGFLSLGTSGVLFVVNDRFRPNPGRAIHAFCHTLPQRWHQMSVMLSAAS 300 SAVGIGAVKPGDGFLSLGTSGVLFVVNDRFRPNPGRAIHAFCHTLPQRWHQMSVMLSAAS Sbjct: 241 SAVGIGAVKPGDGFLSLGTSGVLFVVNDRFRPNPGRAIHAFCHTLPQRWHQMSVMLSAAS 300 Query: 301 CLRWFCRLCSVDEKSLLAEIEQLDEEARNNAPLFLPYLSGERTPHNDPYATGVFHGLTPE 360 CLRWFCRLCSVDEKSLLAEIEQLDE+A NNAPLFLPYLSGERTPHNDPYATGVFHGLTPE Sbjct: 301 CLRWFCRLCSVDEKSLLAEIEQLDEQACNNAPLFLPYLSGERTPHNDPYATGVFHGLTPE 360 Query: 361 HQRAALGYAVLEGVAFGMVDGLDALRAAGTDVAELSLVGGGARSAYWAQLLADALQVRIV 420 HQRAALGYAVLEGVAFGMVDGLDALRAAGTDVAELSLVGGGARSAYWAQLLADALQVRIV Sbjct: 361 HQRAALGYAVLEGVAFGMVDGLDALRAAGTDVAELSLVGGGARSAYWAQLLADALQVRIV 420 Query: 421 THVGSEAGGALGAARLAWLADGGDEAEVCRKPAQQALYQPDAARHAALQVRLQRYRAIYA 480 THVGSEAGGALGAARLAWLADGGDEAEVCRKPAQQALYQPD ARHAALQ RLQRYRAIYA Sbjct: 421 THVGSEAGGALGAARLAWLADGGDEAEVCRKPAQQALYQPDPARHAALQARLQRYRAIYA 480 Query: 481 PFAPLQGE 488 PFAPL GE Sbjct: 481 PFAPLHGE 488 >gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 499 bits (1285), Expect = e-142, Method: Compositional matrix adjust. Identities = 262/483 (54%), Positives = 323/483 (66%), Gaps = 2/483 (0%) Query: 2 YLGIDLGTSEVKAVVIDETGALLALAGSTLTVSRPHPRWSEQSPADWWLATCATVDKLRE 61 YLGIDLGTSEVK V+ DE ++A + L V PHP WSEQ+P WW AT + LR Sbjct: 3 YLGIDLGTSEVKLVLTDEDSNVIASTSARLRVDNPHPLWSEQAPQSWWNATLDAIAALRT 62 Query: 62 QLGSERFGRIRAIGLSGQMHGAVLMDAQDAVLRPAILWSDSRSAPECAELERRAPRLHGI 121 Q + F +R IGLSGQMHGA L+D VLRPAILW+D R+ EC ELE P I Sbjct: 63 Q-APQAFQALRGIGLSGQMHGATLLDRNGNVLRPAILWNDMRAHAECVELEALVPDAVAI 121 Query: 122 AGNLAMPGFTAPKLLWVARHEPQLFARIATVLLPKDWLRFMMTGRKVCDPSDAAGTLWLD 181 GN AMPGFTAPKLLW++++EP ++ I VLLPKD+L + +TG V + SDAAGTLWLD Sbjct: 122 TGNRAMPGFTAPKLLWLSKYEPAVYRAIDKVLLPKDFLGWKLTGEFVSEMSDAAGTLWLD 181 Query: 182 VEGRNWSDELLAASGMRRDQMPALVDGSAVSGTLLPEVAQAWGLRADVIVAGGAGDGAAS 241 V R+WS+ +L A+G+ R MP LV+GSAV+G L E+ + WG+ V+VAGGAGD AAS Sbjct: 182 VARRDWSERMLGATGLDRSHMPRLVEGSAVAGQLRDELRREWGISGPVVVAGGAGDNAAS 241 Query: 242 AVGIGAVKPGDGFLSLGTSGVLFVVNDRFRPNPGRAIHAFCHTLPQRWHQMSVMLSAASC 301 AVGIG ++ GD FLSLG+SGVLF PN + +HAFCH LP +WHQMSV+LSAA+ Sbjct: 242 AVGIGVIRAGDAFLSLGSSGVLFAATAHHAPNAQQGVHAFCHCLPGQWHQMSVILSAAAS 301 Query: 302 LRWFCRLCSVDEKSLLAEIEQLDEEARNNAPLFLPYLSGERTPHNDPYATGVFHGLTPEH 361 L W + + L++ E+L + APLFLPYL+GERTPHND A GV G+TP H Sbjct: 302 LHWLSGVTARPVAELVSGAERLSAGQQAQAPLFLPYLNGERTPHNDAAAKGVLFGMTPAH 361 Query: 362 QRAALGYAVLEGVAFGMVDGLDALRAAGTDVAELSLVGGGARSAYWAQLLADALQVRIVT 421 + A L YAV+EGVAF M DG AL+AAGT + + VGGG+RSA+W L A AL + + Sbjct: 362 ESAHLAYAVMEGVAFAMADGYAALQAAGTTLESAAFVGGGSRSAFWGVLCATALGIPLRR 421 Query: 422 HVGSEAGGALGAARLAWLA-DGGDEAEVCRKPAQQALYQPDAARHAALQVRLQRYRAIYA 480 H G+E G ALGAARL LA G D A VC P Y+PDAAR A LQ RL +YR +Y Sbjct: 422 HAGAEVGAALGAARLGRLARTGEDAASVCIPPEVLEWYRPDAARQAQLQRRLAQYRRLYR 481 Query: 481 PFA 483 A Sbjct: 482 SLA 484 >gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum seropedicae SmR1] Length = 520 Score = 149 bits (376), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 134/454 (29%), Positives = 201/454 (44%), Gaps = 26/454 (5%) Query: 3 LGIDLGTSEVKAVVIDETGALLALAGSTLTVSRPHPRWSEQSPADWWLATCATVDKLREQ 62 LG+D+GT+ K+VV G ++A V P +EQ P + +A + Sbjct: 11 LGVDIGTTSTKSVVFTLDGKVVAQHAEEYPVLCTEPGMAEQDP-EQIVAAALASIAGAVK 69 Query: 63 LGSERFGRIRAIGLSGQMHGAVLMDAQDAVLRPAILWSDSRSA--PECAELERRAPRLHG 120 I + S MH + +DA++ +L +I W D R++ E + E ++ Sbjct: 70 AAKAAPQEIALLSFSAAMHSVIALDAENRLLSNSITWGDIRASVWAERIKHEHDGNAIYR 129 Query: 121 IAGNLAMPGFTAPKLLWVARHEPQLFARIATVLLPKDWLRFMMTGRKVCDPSDAAGTLWL 180 G P KL+W+ +P +F R A + K++L + + G+ V D S A+ T Sbjct: 130 RTGTPVHPMSPLCKLMWMRHEKPDVFHRAARFVGIKEYLLYQLFGQWVVDHSIASATGMF 189 Query: 181 DVEGRNWSDELLAASGMRRDQMPALVDGSAVSGTLLPEVAQAWGLRADVIVAGGAGDGAA 240 ++ W LA G+R DQ+P V + L E+AQ GL D GA DG Sbjct: 190 NLRELAWDQGALALLGVRPDQLPTPVPTTHRLPALSSEMAQRLGLSVDTPFIIGANDGVL 249 Query: 241 SAVGIGAVKPGDGFLSLGTSGVLFVVNDRFRPNP-GRAIHAFCHTLPQR-WHQMSVMLSA 298 S +G+ A++ G +++GTSG + V D R +P GR FC+ L ++ W + + Sbjct: 250 SNLGVNAIEIGHVAVTIGTSGAMRTVIDEPRTDPQGR---LFCYALTEKHWVVGGPVNNG 306 Query: 299 ASCLRWF-----------CRLCSVDEKSLLAEIEQLDEEARNNAP--LFLPYLSGERTPH 345 + RW R VD L I E+ R A LF P+++GER P Sbjct: 307 GNIFRWVRDELATAEAAAAREEGVDPYEALTRIA---EKVRPGAEGLLFHPFMAGERAPL 363 Query: 346 NDPYATGVFHGLTPEHQRAALGYAVLEGVAFGMVDGLDALRAAGTDVAELSLVGGGARSA 405 + G F GL H + + A LEGV F + L AL + GG ARSA Sbjct: 364 WNADLRGSFFGLALHHGKHHMIRAALEGVIFNLYSILPALEELVGPTKRMMATGGFARSA 423 Query: 406 YWAQLLADALQVRIVTHVGSEAGGALGAARL-AW 438 W Q++AD +V E+ LGAA L AW Sbjct: 424 LWRQMMADIFNREVVVPESVES-SCLGAAVLGAW 456 >gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum seropedicae SmR1] Length = 495 Score = 125 bits (315), Expect = 6e-30, Method: Compositional matrix adjust. Identities = 135/499 (27%), Positives = 221/499 (44%), Gaps = 42/499 (8%) Query: 3 LGIDLGTSEVKAVVIDETGALLALAGSTLTVSRPHPRWSEQSPADWWLATCATVDKLREQ 62 L +D GTS ++++ D++GA++A A P P W E P D W + T K+ Q Sbjct: 5 LALDQGTSSSRSIIFDQSGAIVATAQQEFPQIFPQPGWVEHDPMDLWHSQLQTAQKVLAQ 64 Query: 63 LGSERFGRIRAIGLSGQMHGAVLMD-AQDAVLRPAILWSDSRSAPECAELERR--APRLH 119 G + + A+G++ Q V+ + A + A++W D R+ CA L R + Sbjct: 65 SGI-KASELTALGITNQRETTVVWERATGKPVYNAVVWQDRRAEVVCAALRERGLGEAIQ 123 Query: 120 GIAGNLAMPGFTAPKLLWVARHEPQLFAR-------IATVLLPKDWLRFMMTGRK--VCD 170 G + P F+ KL W+ H L AR TV WL + +TG + V D Sbjct: 124 KKTGLVLDPYFSGTKLRWILDHVDGLRARAERGELLFGTV---DSWLVWNLTGGRLHVSD 180 Query: 171 PSDAAGTLWLDVEGRNWSDELLAASGMRRDQMPALVDGSAVSGTLLPEVAQAWGLRADVI 230 S+A+ T+ ++ W +ELL G+ +P + + + G + A L A V Sbjct: 181 VSNASRTMLWNIHTGAWDEELLQWLGIPAAMLPQVQPSAHLYGEI-----DAQHLGAAVK 235 Query: 231 VAGGAGDGAASAVGIGAVKPGDGFLSLGTSGVLFV-VNDRF-RPNPGRAIHAFCHTLPQR 288 + G AGD ++ G V+PG + GT + + D+ + G A C Q+ Sbjct: 236 IGGIAGDQQSALFGQACVRPGMAKNTYGTGCFMLLHTGDQCATSHNGLISTAACQVDEQK 295 Query: 289 WHQM--SVMLSAASCLRWF-----CRLCSVDEKSLLAEIEQLDEEARNNAPLFLPYLSGE 341 + + SV + A ++W S D + L +E + +F+P +G Sbjct: 296 QYALEGSVFIGGA-VVQWLRDGLKAIKTSTDVEGLACSVED------SGGVVFVPSFTGL 348 Query: 342 RTPHNDPYATGVFHGLTPEHQRAALGYAVLEGVAFGMVDGLDAL-RAAGTDVAELSLVGG 400 P+ +P A G GL+ A + A LE +AF L+A+ R A + + EL + GG Sbjct: 349 GAPYWNPSAKGAIVGLSRGSTVAHIARAALESIAFQSTALLNAMVRDAVSPITELRVDGG 408 Query: 401 GARSAYWAQLLADALQVRIVTHVGSEAGGALGAARLAWLADG--GDEAEVCRKPAQQALY 458 A + Q AD L + ++ +E ALGAA LA +A G D +E+ + + + Sbjct: 409 AAANNLLLQFQADLLGIPVIRPQVTET-TALGAAYLAGIATGVYRDLSELSSQWRMEHTF 467 Query: 459 QPDAARHAALQVRLQRYRA 477 +P R A Q R+ + A Sbjct: 468 EPSIGRDQA-QARIHAWEA 485 >gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herbaspirillum seropedicae SmR1] Length = 548 Score = 86.3 bits (212), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 128/483 (26%), Positives = 182/483 (37%), Gaps = 46/483 (9%) Query: 3 LGIDLGTSEVKAVVIDETGALLALAGSTLTVSRPHPRWS-EQSPADWWLATCATVDKLRE 61 +GID+GT +A V D G LLA A + + R R EQS A W A C V Sbjct: 6 IGIDVGTGSARAGVFDPQGQLLASARHDIDLFRDERRARVEQSSAQIWEAVCQAVRAAVT 65 Query: 62 QLGSERFGRIRAIGLSGQMHGAVLMDAQDAVLRPA------ILWSDSRSAPECAELERRA 115 + G + + IG+ V+ A V P I+W D R+ + + R Sbjct: 66 KSGIDP-AAVAGIGVDATC-SLVVQGASAGVGDPGHPERDVIVWMDHRALEQAQRINRMG 123 Query: 116 PRLHGIAGNLAMPGFTAPKLLWVARHEPQLFARIATVLLPKDWLRFMMTG---RKVCDPS 172 + G + P PKLLW+ + P+++A A D+L + TG R C + Sbjct: 124 HAVLSYVGGVISPEMETPKLLWLKENLPEVYASAAHFFDLTDFLTWKATGSLQRSSCTVT 183 Query: 173 DAAGTL-----WLDVEGRNWSDELLAASGMRRDQMPALVDGSAVSGTLLPEVAQAWGLRA 227 L W R LA +G R + G+ ++G L AQA GLR Sbjct: 184 CKWTYLAHEAGWDASYFRQIGLGDLAEAGFARIGAEVVWPGTPLAGGLTQTAAQAMGLRP 243 Query: 228 DVIVAGGAGDGAASAVGIGAVKPGDGFLS------LGTSGVLFVVNDRFRPNPGRAIHAF 281 + VA G D A VG A + G G + GTS + PG + Sbjct: 244 GIAVAAGLIDAHAGGVGTVAARGGQGDAAACMAYVFGTSSCTMTSHADPVLVPGVWGPYY 303 Query: 282 CHTLPQRWHQMSVMLSAASCLRWFCRLCSVDEKS------------------LLAEIEQL 323 P W +A + + RL ++ LA ++ Sbjct: 304 KAMAPGMWLNEGGQSAAGAAIDHLLRLHPATPQAREEAARAQMELPQWLAQQALAAVDHP 363 Query: 324 DEEARNNAPL-FLPYLSGERTPHNDPYATGVFHGLTPEH---QRAALGYAVLEGVAFGMV 379 E L +P G R+P DP A GL EH AL A L + +G+ Sbjct: 364 SEAVWLAGQLTVVPEFLGNRSPLADPQARAALVGLGMEHDIDSLVALYVAGLCSLGYGLR 423 Query: 380 DGLDALRAAGTDVAELSLVGGGARSAYWAQLLADALQVRIVTHVGSEAGGALGAARLAWL 439 ++A A G V +S+ GG QLLADA + + E LG+A LA + Sbjct: 424 QIIEAQAACGVRVQSISVSGGAGVHPLTRQLLADATGMPVEITASPEP-VLLGSAMLAAV 482 Query: 440 ADG 442 A G Sbjct: 483 AAG 485 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,545,822 Number of extensions: 64359 Number of successful extensions: 230 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 220 Number of HSP's successfully gapped: 5 Length of query: 490 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 401 Effective length of database: 1,198,455 Effective search space: 480580455 Effective search space used: 480580455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)