BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0045.p (848 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309767|ref|YP_003773859.1| valyl-tRNA synthetase [Herbaspi... 1500 0.0 gi|300310736|ref|YP_003774828.1| isoleucyl-tRNA synthetase [Herb... 119 8e-28 gi|300309930|ref|YP_003774022.1| leucyl-tRNA synthetase [Herbasp... 85 3e-17 >gi|300309767|ref|YP_003773859.1| valyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 971 Score = 1500 bits (3883), Expect = 0.0, Method: Compositional matrix adjust. Identities = 720/754 (95%), Positives = 723/754 (95%), Gaps = 28/754 (3%) Query: 1 MELAKSFEPAEIEKFWREEWEKRGYFAATTDAEKPSFSIQLPPPNVTGTLHMGHAFNQTI 60 MELAKSFEPAEIEKFWREEWEKRGYFAATTDAEKPSFSIQLPPPNVTGTLHMGHAFNQTI Sbjct: 36 MELAKSFEPAEIEKFWREEWEKRGYFAATTDAEKPSFSIQLPPPNVTGTLHMGHAFNQTI 95 Query: 61 MDGLTRYHRMRGYNTAWIPGTDHAGIATQIVVERQLDAQKVSRHDLGREKFVEKVWEWKE 120 MDGLTRYHRMRGYNTAWIPGTDHAGIATQIVVERQLDAQKVSRHDLGREKFVEKVWEWKE Sbjct: 96 MDGLTRYHRMRGYNTAWIPGTDHAGIATQIVVERQLDAQKVSRHDLGREKFVEKVWEWKE 155 Query: 121 KSGSTITGQMRRMGASTDWAREYFTMDPKMSKAVTEVFVRLYEQGLIYRGKRLVNWDPVL 180 KSGSTITGQMRRMGASTDWAREYFTMDPKMSK VTEVFVRLYEQGLIYRGKRLVNWDPVL Sbjct: 156 KSGSTITGQMRRMGASTDWAREYFTMDPKMSKVVTEVFVRLYEQGLIYRGKRLVNWDPVL 215 Query: 181 GTAVSDLEVVSEEEDGSMWHIKY----------------------------PTDERYAAL 212 GTAVSDLEVVSEEEDGSMWHIKY PTDERYAAL Sbjct: 216 GTAVSDLEVVSEEEDGSMWHIKYPLADGSGHIVVATTRPETMLGDVAVAVDPTDERYAAL 275 Query: 213 VGKMIKLPLVGREIPIIKDEYVDKEFGTGCVKITPAHDFNDYQVGARHNLEMISVFTLKA 272 VGKMIKLPLVGREIPIIKDEYVDKEFGTGCVKITPAHDFNDYQVGARHNLEMISVFTLKA Sbjct: 276 VGKMIKLPLVGREIPIIKDEYVDKEFGTGCVKITPAHDFNDYQVGARHNLEMISVFTLKA 335 Query: 273 TINENSPEKYQGLDRFEARKQIVADLDALGLLELVKPHKLMVPRGDRTGVVIEPMLTDQW 332 TINEN+PEKYQGLDRFEARKQIVADLDALGLLE VKPHKLMVPRGDRTGVVIEPMLTDQW Sbjct: 336 TINENAPEKYQGLDRFEARKQIVADLDALGLLEQVKPHKLMVPRGDRTGVVIEPMLTDQW 395 Query: 333 FVAMSKPAPEGTFFPGKSIAETALEKVSSGEIKFVPENWTNTYNQWLNNIQDWCISRQLW 392 FVAMSKPAPEGTFFPGKSIAETALEKVSSGEIKFVPENWTNTYNQWLNNIQDWCISRQLW Sbjct: 396 FVAMSKPAPEGTFFPGKSIAETALEKVSSGEIKFVPENWTNTYNQWLNNIQDWCISRQLW 455 Query: 393 WGHQIPAWYDEDGGIYVARTEEEAKKQAGGKAVKRDEDVLDTWFSSALVPFSTLGWPEET 452 WGHQIPAWYDEDGGIYVARTEEEA+ QAGG+AVKRDEDVLDTWFSSALVPFSTLGWPEET Sbjct: 456 WGHQIPAWYDEDGGIYVARTEEEARAQAGGRAVKRDEDVLDTWFSSALVPFSTLGWPEET 515 Query: 453 PDYKLFLPSSVLVTGFDIIFFWVARMVMMTTHFTGKVPFDTVYVHGLVRDASGQKMSKSK 512 PDYKLFLPSSVLVTGFDIIFFWVARMVMMTTHFTGKVPFDTVYVHGLVRDASGQKMSKSK Sbjct: 516 PDYKLFLPSSVLVTGFDIIFFWVARMVMMTTHFTGKVPFDTVYVHGLVRDASGQKMSKSK 575 Query: 513 GNTLDPIDLIDGIGVDELVAKRTVGLMNPKQAASIEKATRKEFADGIPAFGTDALRFTFA 572 GNTLDPIDLIDGIGVDELVAKRTVGLMNPKQAASIEKATRKEFADGIPAFGTDALRFTFA Sbjct: 576 GNTLDPIDLIDGIGVDELVAKRTVGLMNPKQAASIEKATRKEFADGIPAFGTDALRFTFA 635 Query: 573 SLATLGRNINFDLNRCEGYRNFCNKLWNATRFVLMNTEGQDCGFDGHEKGVCDKEKLQFS 632 SLATLGRNINFDLNRCEGYRNFCNKLWNATRFVLMNTEGQDCGFDGHEKGVCDKEKLQFS Sbjct: 636 SLATLGRNINFDLNRCEGYRNFCNKLWNATRFVLMNTEGQDCGFDGHEKGVCDKEKLQFS 695 Query: 633 KADRWIVSLLQRTEAEVEKGFADYRFDNIATAIYKFVWDEYCDWYLEMAKAQIQSGNEAQ 692 KADRWIVSLLQRTEAEVEKGFADYRFDNIATAIYKFVWDEYCDWYLEMAKAQIQSGNEAQ Sbjct: 696 KADRWIVSLLQRTEAEVEKGFADYRFDNIATAIYKFVWDEYCDWYLEMAKAQIQSGNEAQ 755 Query: 693 QRATRRTLLRVLESVLRLAHPVLPFITEQLWQSV 726 QRATRRTLLRVLESVLRLAHPVLPFITEQLWQSV Sbjct: 756 QRATRRTLLRVLESVLRLAHPVLPFITEQLWQSV 789 >gi|300310736|ref|YP_003774828.1| isoleucyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 964 Score = 119 bits (299), Expect = 8e-28, Method: Compositional matrix adjust. Identities = 125/530 (23%), Positives = 218/530 (41%), Gaps = 88/530 (16%) Query: 224 REIPIIKDEYVDKEFGTGCVKITPAHDFNDYQVGARHNLEMISVFTLKATINENSPEK-- 281 R P+ EYV + GTG V +PA+ D+ H ++ + L + + Sbjct: 337 RLSPVFLGEYVTADSGTGIVHSSPAYGIEDFVSCKAHGMKDDDI--LNPVMGDGRYASWL 394 Query: 282 --YQGLDRFEARKQIVADLDALG-LLELVK-PHKLMVPRGDRTGVVIEPMLTDQWFVAMS 337 + GL +EA K I L+ +G L +L+ H M ++ ++ T QWF +M Sbjct: 395 PLFAGLTIWEASKPICDKLEEIGSLFKLIMFSHSYMHCWRHKSPIIYRA--TSQWFASMD 452 Query: 338 KPAPEGTFFPGKSIAETALEKVSSGEIKFVPENWTNTYNQWLNNIQDWCISRQLWWGHQI 397 +G G S+ +TAL+ + + F P + + N DW +SRQ WG + Sbjct: 453 NTPKDG----GPSLRQTALQGIE--DTAFFPGWGKARLHGMIANRPDWTLSRQRQWGVPM 506 Query: 398 PAWYDEDGGIYVARTEE--------------------EAKKQAGGKAVK--RDEDVLDTW 435 + ++ G RT E + K+ G +A + +++D LD W Sbjct: 507 AFFVHKETGELHPRTPELLEQIAQRIEQGGIEAWQALDPKELLGDEADQYLKNKDTLDVW 566 Query: 436 FSSALVPFSTLGWPEETPDYKLFLPSSVLVTGFDIIFFWVARMVMMTTHFTGKVPFDTVY 495 F S + L + +L P+ + + G D W ++ ++ G+ P+ + Sbjct: 567 FDSGSTHQTVL---RGSHRQELQFPADLYLEGSDQHRGWFHSSLLTSSMLNGRAPYKALL 623 Query: 496 VHGLVRDASGQKMSKSKGNTLDPIDLIDGIGVDELVAKRTVGLMNPKQAASIEKATRKEF 555 HG V D G+KMSKS N ++P + D +G + + + V + SI + K Sbjct: 624 THGFVVDGEGRKMSKSLKNGVEPQKVADSLGAE--ILRLWVASSDYSGEISISEEILK-- 679 Query: 556 ADGIPAFGTDALRFTFASLATLGRNINFDLNRCEGYRNFCNKLWNATRFVLMNTEGQDCG 615 R T E YR N L RF+L NT Sbjct: 680 ------------RVT------------------EAYRRIRNTL----RFLLANT----SD 701 Query: 616 FDGHEKGVCDKEKLQFSKADRWIVSLLQRTEAEVEKGFADYRFDNIATAIYKFVWDEYCD 675 FD + V E L+ DR+ ++ + R + EV F Y F + + + ++ Sbjct: 702 FDPAQHAVPMAELLEI---DRYAIANMARLQGEVLAHFETYEFHPVVAKLQMYCSEDLGG 758 Query: 676 WYLEMAKAQIQSG--NEAQQRATRRTLLRVLESVLRLAHPVLPFITEQLW 723 +YL++ K ++ + + A +R+ + + + +S+LRL P+L F E+ W Sbjct: 759 FYLDILKDRLYTSGVDSAARRSAQTAVWHITQSLLRLMAPILSFTAEEAW 808 Score = 76.3 bits (186), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 46/201 (22%), Positives = 92/201 (45%), Gaps = 8/201 (3%) Query: 10 AEIEKFWREEWEKRGYFAATTDAE--KPSFSIQLPPPNVTGTLHMGHAFNQTIMDGLTRY 67 A+ E W ++W+++ + A +P F + PP G +H+GHA N+ + D + + Sbjct: 39 AKREPQWVKQWQEKKVYERIRKASAGRPKFILHDGPPYANGEIHLGHAVNKILKDMIVKA 98 Query: 68 HRMRGYNTAWIPGTDHAGIATQIVVERQLDAQKVSRHDLGREKFVEKVWEWKEKSGSTIT 127 M G++ ++PG D G+ +I +E++ +L + K + + + Sbjct: 99 RNMAGFDAQYVPGWDCHGMPIEIQIEKKYG------KNLPVAEVQAKARAYANEQIALQK 152 Query: 128 GQMRRMGASTDWAREYFTMDPKMSKAVTEVFVRLYEQGLIYRGKRLVNWDPVLGTAVSDL 187 R+G +W Y TM+ ++ E+G +YRG + VNW G+A+++ Sbjct: 153 KDFIRLGVLGEWDNPYKTMNFSNEADELRALGKILEKGYVYRGLKPVNWCFDCGSALAEA 212 Query: 188 EVVSEEEDGSMWHIKYPTDER 208 EV +++ + +P E Sbjct: 213 EVEYQDKRDPAIDVGFPFAEH 233 >gi|300309930|ref|YP_003774022.1| leucyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 882 Score = 84.7 bits (208), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 66/239 (27%), Positives = 102/239 (42%), Gaps = 42/239 (17%) Query: 3 LAKSFEPAEIEKFWREEWEKRGYFAATTDA------EKPSFSIQLPPPNVTGTLHMGHAF 56 + + PAE+E+ ++ W + +A +K F P +G LHMGH Sbjct: 1 MQDKYSPAEVEQSAQQHWNAIDAYKTVENARDKQGRDKKKFYACSMLPYPSGKLHMGHVR 60 Query: 57 NQTIMDGLTRYHRMRGYNTAWIPGTDHAGIATQIVVERQLDAQKVSRHDLGREKFVEKVW 116 N TI D + RY RM GYN G D G+ E A V Sbjct: 61 NYTINDVMYRYLRMNGYNVLMPMGWDAFGMPA----ENAAMANGVPPA------------ 104 Query: 117 EWKEKSGSTITGQMRRMGASTDWAREYFTMDPKMSKAVTEVFVRLYEQGLIYRGKRLVNW 176 +W + + QM+ MG + DW+RE P+ K +F+++ E+G+IY+ VNW Sbjct: 105 QWTYSNIDYMKSQMQSMGLAIDWSREMTACRPEYYKWNQWMFLKMLEKGIIYKKTGTVNW 164 Query: 177 DPVLGTAVSDLEVVSEEEDGSMWHIKYPTDERYAALVGKMIKLPLVGREIPIIKDEYVD 235 DP+ T +++ +V+ DG W R A++ K REIP+ + D Sbjct: 165 DPIDQTVLANEQVI----DGRGW--------RSGAVIEK--------REIPMYYAKITD 203 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,567,312 Number of extensions: 104909 Number of successful extensions: 262 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 258 Number of HSP's successfully gapped: 4 Length of query: 848 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 755 Effective length of database: 1,179,515 Effective search space: 890533825 Effective search space used: 890533825 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 135 (56.6 bits)