BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0005.p (459 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309729|ref|YP_003773821.1| biotin carboxylase [Herbaspiril... 932 0.0 gi|300313399|ref|YP_003777491.1| acyl-CoA carboxylase biotin sub... 403 e-113 gi|300313973|ref|YP_003778065.1| acetyl/propionyl-CoA carboxylas... 375 e-105 gi|300313753|ref|YP_003777845.1| urea amidolyase [Herbaspirillum... 317 1e-87 >gi|300309729|ref|YP_003773821.1| biotin carboxylase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 932 bits (2408), Expect = 0.0, Method: Compositional matrix adjust. Identities = 452/459 (98%), Positives = 457/459 (99%) Query: 1 MFEKILIANRGEIALRIQRACRELGIKTVVVHSEADREAKYVKLADESVCIGPAPSALSY 60 MFEKILIANRGEIALRIQRACRELGIKTVVVHSEADREAKYVKLADESVCIGPAPS LSY Sbjct: 1 MFEKILIANRGEIALRIQRACRELGIKTVVVHSEADREAKYVKLADESVCIGPAPSTLSY 60 Query: 61 LNMPAIISAAEVTDAQAIHPGYGFLSENADFAERVEQSGFVFIGPRAENIRMMGDKVSAK 120 LNMPAIISAAEVTDAQAIHPGYGFLSENADFAERVE+SGFVFIGPRAENIRMMGDKVSAK Sbjct: 61 LNMPAIISAAEVTDAQAIHPGYGFLSENADFAERVEKSGFVFIGPRAENIRMMGDKVSAK 120 Query: 121 QAMIRAGVPCVPGSEGALPDNPKEIVQIARKIGYPVIIKAAGGGGGRGMRVVHTEAALIN 180 QAMIRAGVPCVPGS+GALPDNPKEIVQIARKIGYPVIIKAAGGGGGRGMRVVHTEAALIN Sbjct: 121 QAMIRAGVPCVPGSDGALPDNPKEIVQIARKIGYPVIIKAAGGGGGRGMRVVHTEAALIN 180 Query: 181 AVTMTKTEAGAAFGNPEVYMEKYLENPRHVEIQILADEHKQAVWLGERDCSMQRRHQKVI 240 AVTMTKTEAGAAFGNPEVYMEKYLENPRHVEIQILADEHKQA+WLGERDCSMQRRHQKVI Sbjct: 181 AVTMTKTEAGAAFGNPEVYMEKYLENPRHVEIQILADEHKQAIWLGERDCSMQRRHQKVI 240 Query: 241 EEAPAPGIPRKIIEKIGERCAEACRKMNYRGAGTFEFLYENEEFYFIEMNTRVQVEHPVT 300 EEAPAPGIPRKIIEKIGERCAEACRKMNYRGAGTFEFLYENEEFYFIEMNTRVQVEHPVT Sbjct: 241 EEAPAPGIPRKIIEKIGERCAEACRKMNYRGAGTFEFLYENEEFYFIEMNTRVQVEHPVT 300 Query: 301 EMITGVDIVQEQIRIAAGEKLRYRQRDIELKGHAIECRINAEDPFKFIPSPGRITAWHVP 360 EMITGVDIVQEQIRIAAGEKLRYRQRDIELKGHAIECRINAEDPFKFIPSPGRITAWHVP Sbjct: 301 EMITGVDIVQEQIRIAAGEKLRYRQRDIELKGHAIECRINAEDPFKFIPSPGRITAWHVP 360 Query: 361 GGPGIRVDSHAYSGYFVPPNYDSMVGKVIAYGATREQAIRRMQIALSEMVVEGISTNIPL 420 GGPGIRVDSHAYSGYFVPPNYDSMVGKVIAYGATREQAIRRMQIALSEMVVEGISTNIPL Sbjct: 361 GGPGIRVDSHAYSGYFVPPNYDSMVGKVIAYGATREQAIRRMQIALSEMVVEGISTNIPL 420 Query: 421 HRELMVDARFFEGGTNIHYLEHKLSERPTAPEADKSIKK 459 HRELMVDARFFEGGTNIHYLEHKLSERP +PEADK+IKK Sbjct: 421 HRELMVDARFFEGGTNIHYLEHKLSERPASPEADKAIKK 459 >gi|300313399|ref|YP_003777491.1| acyl-CoA carboxylase biotin subunit [Herbaspirillum seropedicae SmR1] Length = 462 Score = 403 bits (1036), Expect = e-113, Method: Compositional matrix adjust. Identities = 220/449 (48%), Positives = 301/449 (67%), Gaps = 5/449 (1%) Query: 2 FEKILIANRGEIALRIQRACRELGIKTVVVHSEADREAKYVKLADESVCIGPAPSALSYL 61 K+LIANRGEIA+RI RA + LGI TV S+AD ++ ++ADE IGPA + SYL Sbjct: 5 LRKLLIANRGEIAVRIIRAAQALGITTVAACSDADTDSLAARMADEVQRIGPARADRSYL 64 Query: 62 NMPAIISAAEVTDAQAIHPGYGFLSENADFAERVEQSGFVFIGPRAENIRMMGDKVSAKQ 121 N+ A++ AA + A A+HPGYGFLSENA FAE V +G +F+GP+A+ IR MGDK A++ Sbjct: 65 NVDALLKAARDSGADALHPGYGFLSENAAFAEAVNAAGLIFVGPQADTIRRMGDKAEARR 124 Query: 122 AMIRAGVPCVPGSEGALPDNPKEIVQIARKIGYPVIIKAAGGGGGRGMRVVHTEAALINA 181 AGVP VPGS G L D + A +IGYP++IKA+ GGGGRG+R+ L Sbjct: 125 TAAAAGVPVVPGSAGELEDLATALA-CADEIGYPLLIKASAGGGGRGIRMARDATELARE 183 Query: 182 VTMTKTEAGAAFGNPEVYMEKYLENPRHVEIQILADEHKQAVWLGERDCSMQRRHQKVIE 241 + + EA AAFG+ VY+E+++ RH+E+QIL D + AV L ER+CS+QRR QKV+E Sbjct: 184 FPLAQAEAQAAFGSAAVYLERFIRRARHIEVQILGDGER-AVHLFERECSLQRRRQKVLE 242 Query: 242 EAPAPGIPRKIIEKIGERCAEACRKMNYRGAGTFEFLYENE--EFYFIEMNTRVQVEHPV 299 EAP+P + + + + +++YRGAGT E+L+++E EF+FIEMNTR+QVEHPV Sbjct: 243 EAPSPALTPAQRQALCDSAVRLAERLHYRGAGTLEYLFDDESGEFFFIEMNTRIQVEHPV 302 Query: 300 TEMITGVDIVQEQIRIAAGEKLRYRQRDIELKGHAIECRINAEDPFK-FIPSPGRITAWH 358 TEMITG+D+VQ +RIA GE L +Q DI ++G A+E RINAEDP + F P PG + Sbjct: 303 TEMITGIDLVQAMLRIAGGEPLGLQQSDIRMQGAALEMRINAEDPERNFFPCPGTVAELQ 362 Query: 359 VPGGPGIRVDSHAYSGYFVPPNYDSMVGKVIAYGATREQAIRRMQIALSEMVVEGISTNI 418 P G GIRV+SH Y+GY +PP YDS++ K++ +G R QAI R Q A+ + G++T + Sbjct: 363 WPQGEGIRVESHLYAGYRIPPYYDSLLAKLVVHGKDRAQAIARAQAAVLATRITGMATTL 422 Query: 419 PLHRELMVDARFFEGGTNIHYLEHKLSER 447 LH+ L+ DAR + LE L+ER Sbjct: 423 SLHQWLLADARVQSARFDTGALETWLAER 451 >gi|300313973|ref|YP_003778065.1| acetyl/propionyl-CoA carboxylase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 671 Score = 375 bits (963), Expect = e-105, Method: Compositional matrix adjust. Identities = 229/472 (48%), Positives = 299/472 (63%), Gaps = 19/472 (4%) Query: 1 MFEKILIANRGEIALRIQRACRELGIKTVVVHSEADREAKYVKLADESVCIGPAPSALSY 60 MF KILIANRGEIA R+ R LGI TV V+S+AD AK+V DESV +GPAP+ SY Sbjct: 1 MFTKILIANRGEIACRVAATARRLGIATVAVYSDADARAKHVAACDESVRLGPAPARESY 60 Query: 61 LNMPAIISAAEVTDAQAIHPGYGFLSENADFAERVEQSGFVFIGPRAENIRMMGDKVSAK 120 L II+AA T AQAIHPGYGFLSENA FA+ Q+G VFIGP A I MG K +AK Sbjct: 61 LCAEKIIAAALATGAQAIHPGYGFLSENAGFAQACAQAGLVFIGPPASAIEAMGSKAAAK 120 Query: 121 QAMIRAGVPCVPGSEGALPDNPKEIVQIARKIGYPVIIKAAGGGGGRGMRVVHTEAALIN 180 M +A VP VPG G ++ Q A +IGYPV++KA+ GGGG+GMRVV Sbjct: 121 ALMEQAAVPLVPGYHGEEQESGLLHSQ-ADRIGYPVLLKASAGGGGKGMRVVERSEDFDA 179 Query: 181 AVTMTKTEAGAAFGNPEVYMEKYLENPRHVEIQILADEHKQAVWLGERDCSMQRRHQKVI 240 A+ K EA A+FG+ V +EKYL PRH+EIQ+ AD H V+L ERDCS+QRRHQKV+ Sbjct: 180 ALASCKREAIASFGDDRVLVEKYLTRPRHIEIQVFADGHGHCVYLFERDCSVQRRHQKVL 239 Query: 241 EEAPAPGIPRKIIEKIGERCAEACRKMNYRGAGTFEFLYENE-EFYFIEMNTRVQVEHPV 299 EEAPAPG+ + +GE A R + Y GAGT EF+ + FYF+EMNTR+QVEHPV Sbjct: 240 EEAPAPGMSEERRAAMGEAAVAAARAVGYVGAGTVEFIANQDGSFYFMEMNTRLQVEHPV 299 Query: 300 TEMITGVDIVQEQIRIAAGEKLRYRQRDIELKGHAIECRINAEDPFK-FIPSPGRIT--- 355 TEMITG+D+V+ Q+R+AAG+ L +Q + + GHAIE RI AE+P K F+P+ G +T Sbjct: 300 TEMITGLDLVEWQLRVAAGQALPLKQEQLRIHGHAIEARIYAENPEKGFLPAIGTLTRMR 359 Query: 356 ---------AWHVPGGPG-IRVDSHAYSGYFVPPNYDSMVGKVIAYGATREQAIRRMQIA 405 PG P +R+D+ G + P YD M+ K+I +G R+ A+R MQ A Sbjct: 360 SAAAVEFRLGQSAPGDPAHVRIDAGVREGDAISPFYDPMIAKLIVWGPDRDAALRNMQRA 419 Query: 406 LSEMVVEGISTNIPLHRELMVDARFFEGGTNIHYLE-HK--LSERPTAPEAD 454 L++ V G++TNI + L+ F + +E H+ L P++P+ + Sbjct: 420 LAQYQVVGLATNIAFLQRLVAGQAFSTAALDTGLIERHRDTLFPPPSSPDTE 471 >gi|300313753|ref|YP_003777845.1| urea amidolyase [Herbaspirillum seropedicae SmR1] Length = 1207 Score = 317 bits (812), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 183/449 (40%), Positives = 274/449 (61%), Gaps = 9/449 (2%) Query: 1 MFEKILIANRGEIALRIQRACRELGIKTVVVHSEADREAKYVKLADESVCIGPAPSALSY 60 MF+ +LIANRG IA RI R R L + V V+SEAD + +V AD ++ +G P+A +Y Sbjct: 1 MFDHLLIANRGAIACRILRTLRTLDVTGVCVYSEADLGSLHVLQADRAISLGEGPAAQTY 60 Query: 61 LNMPAIISAAEVTDAQAIHPGYGFLSENADFAERVEQSGFVFIGPRAENIRMMGDKVSAK 120 L + I++AA + AQAIHPGYGFLSENA FAE E +G F+GP +R+ G K +A+ Sbjct: 61 LAVDKILAAARDSGAQAIHPGYGFLSENAAFAEACEAAGIAFVGPTPAQLRIFGLKHTAR 120 Query: 121 QAMIRAGVPCVPGSEGALPDNPKEIVQIARKIGYPVIIKAAGGGGGRGMRVVHTEAALIN 180 GVP + G++ L ++ + + A ++GYPV++K+ GGGG GMRV ++A L + Sbjct: 121 ALAREQGVPMLEGTD--LLESIDDALAAASRVGYPVMLKSTAGGGGIGMRVCRSDAELAD 178 Query: 181 AVTMTKTEAGAAFGNPEVYMEKYLENPRHVEIQILADEHKQAVWLGERDCSMQRRHQKVI 240 A + F + V++EKY+E RH+E+Q+ D + + LG RDCS+QRR+QKV+ Sbjct: 179 AFDSVRRLGQNNFSDAGVFIEKYIERARHLEVQVFGDGRGEVIALGVRDCSVQRRNQKVL 238 Query: 241 EEAPAPGIPRKIIEKIGERCAEACRKMNYRGAGTFEFLYEN--EEFYFIEMNTRVQVEHP 298 EE PAP +P + E + + + +NYR AGT EF+Y++ +FYF+E+NTR+QVEH Sbjct: 239 EETPAPNLPDGMAEALCAAAIKLAKAVNYRSAGTVEFVYDSLAGQFYFLEVNTRLQVEHG 298 Query: 299 VTEMITGVDIVQEQIRIAAGE--KLRYRQRDIELKGHAIECRINAEDPFK-FIPSPGRIT 355 VTE + GVD+V+ I +AAG+ L ++ +GHAI+ R+ AEDP + F P PG +T Sbjct: 299 VTEQVWGVDLVRWMIELAAGDLPPLAQLAAGLQPRGHAIQARLYAEDPGRQFQPCPGLLT 358 Query: 356 AWHVP--GGPGIRVDSHAYSGYFVPPNYDSMVGKVIAYGATREQAIRRMQIALSEMVVEG 413 A P G +R+D+ +G + P +D M+ K+I++ TRE A + AL ++ G Sbjct: 359 AVDFPPVDGQALRIDTWVEAGCEIAPYFDPMIAKLISWQPTREAARLALDAALRATLLYG 418 Query: 414 ISTNIPLHRELMVDARFFEGGTNIHYLEH 442 + TN R+++ D F G LE Sbjct: 419 VETNQHYLRQILADVPFASGQPWTRCLEQ 447 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,360,750 Number of extensions: 53392 Number of successful extensions: 163 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 154 Number of HSP's successfully gapped: 4 Length of query: 459 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 371 Effective length of database: 1,203,190 Effective search space: 446383490 Effective search space used: 446383490 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)