BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0124.p (488 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase ... 860 0.0 gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 176 3e-45 gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 169 6e-43 gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 148 1e-36 gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 136 4e-33 >gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 623 Score = 860 bits (2222), Expect = 0.0, Method: Compositional matrix adjust. Identities = 444/504 (88%), Positives = 458/504 (90%), Gaps = 33/504 (6%) Query: 13 DVAVFARRFDAALYLGVCDKIVMSHNMFDAALYLGVCDKIVPGLLIGALHFGHLPAVFVP 72 ++++F+R DA + + I +SHNMFDAALYLGVCDKIVPGLLIGALHFGHLPAVFVP Sbjct: 125 ELSLFSR--DA---IAMATAIALSHNMFDAALYLGVCDKIVPGLLIGALHFGHLPAVFVP 179 Query: 73 AGPMTSGMSNSDKVKIRQLYTAGKIGRAELLEAESKSYHGAGTCTFYGTANSNQMLMEAM 132 AGPMTSGMSNSDKVKIRQLYTAGKIGRAELLEAESKSYHGAGTCTFYGTANSNQMLMEAM Sbjct: 180 AGPMTSGMSNSDKVKIRQLYTAGKIGRAELLEAESKSYHGAGTCTFYGTANSNQMLMEAM 239 Query: 133 GLHLPGAAFITPNTPLRDALTAAAAKQAAKITDLGSQYTPVGRMIDEKSIVNAIVALHAT 192 GLHLPGAAFITPNTPLRDALTAAAAKQAAKITDLG+QYTPVGRMIDEKSIVNAIVALHAT Sbjct: 240 GLHLPGAAFITPNTPLRDALTAAAAKQAAKITDLGTQYTPVGRMIDEKSIVNAIVALHAT 299 Query: 193 GGSTNHTLHLVAIAKAAGIVIDWDDFDKLSAVVPLITKIYPNGTADVNHFHAAGGTGFVL 252 GGSTNHTLHLVAIAKAAGIVIDWDDFDKLSAVVPLITKIYPNGTADVNHFHAAGGTGFVL Sbjct: 300 GGSTNHTLHLVAIAKAAGIVIDWDDFDKLSAVVPLITKIYPNGTADVNHFHAAGGTGFVL 359 Query: 253 RELIDAGLMHDDVTTILGQGLRQHCKEPMLAAEGENGRPGIVTWHDAPATSGDEGVLGTV 312 RELIDAGLMHDDVTTILGQGLRQHCKEPMLAAEGENGRPGIVTWHDAPA SGDEGVLGTV Sbjct: 360 RELIDAGLMHDDVTTILGQGLRQHCKEPMLAAEGENGRPGIVTWHDAPAQSGDEGVLGTV 419 Query: 313 AKPFAADGGLKLLQGNLGRAVIKISAVKPEHRVVEAPAVVFHSQEAFMAAFKAGELDRDF 372 AKPFAADGGLKLLQGNLGRAVIKISAVKPEHRVVEAPAVVFHSQEAFMAAFKAG+LDRDF Sbjct: 420 AKPFAADGGLKLLQGNLGRAVIKISAVKPEHRVVEAPAVVFHSQEAFMAAFKAGQLDRDF 479 Query: 373 VAVITFQGPRANGMPELHSLTPALGILQDKGRHVALVTDGRMSGASGKVPAAIHVTPEVL 432 VAVITFQGPRANGMPELH+LTPALGILQDKGRHVALVTDGRMSGASGKVPAAIHVTPEVL Sbjct: 480 VAVITFQGPRANGMPELHALTPALGILQDKGRHVALVTDGRMSGASGKVPAAIHVTPEVL 539 Query: 433 GGGPLGRVRTGDIIRLDADAGTLQAKVEAAEWEAPPWDX--------------------- 471 GGGPLGRVR GDIIRLDA+AGTL+AKV+A EW A D Sbjct: 540 GGGPLGRVRDGDIIRLDAEAGTLEAKVDATEWAARSQDSSDLSGNQHGMGRELFSLFRNA 599 Query: 472 -------GATFGLPPIFEKEKVTV 488 GATFGLPPIFEKEKVTV Sbjct: 600 VGTAEEGGATFGLPPIFEKEKVTV 623 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 176 bits (446), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 146/441 (33%), Positives = 214/441 (48%), Gaps = 43/441 (9%) Query: 38 NMFDAALYLGVCDKIVPGLLIGALHFGHLPAVFVPAGPMTSGMSNSDKV-------KIRQ 90 N DA + L CDK P LL+GA +PA+ V GPM +G + ++ + Sbjct: 117 NPIDAVVLLTGCDKTTPALLMGAASC-DVPAIVVTGGPMLNGKHQGRDIGSGTVVWQLSE 175 Query: 91 LYTAGKIGRAELLEAESKSYHGAGTCTFYGTANSNQMLMEAMGLHLPGAAFITPNTPLRD 150 AG+I + + AE+ AGTC GTA++ + E++G+ LP A I P D Sbjct: 176 QVKAGEITIHDFMAAEAGMSRSAGTCNTMGTASTMACMAESLGVSLPHNAAI----PAVD 231 Query: 151 ALTAAAAKQAA-KITDLGSQYTPVGRMIDEKSIVNAIVALHATGGSTNHTLHLVAIAKAA 209 A A + +I D+ + + +++ K+ NAI A GGSTN +HL AIA Sbjct: 232 ARRYVLAHLSGMRIVDMVWEGLTLSKILTRKAFENAIRTNAAIGGSTNAVIHLKAIAGRI 291 Query: 210 GIVIDWDDFDKLSAVVPLITKIYPNGTADVNHFHAAGGTGFVLRELIDAGLM-HDDVTTI 268 G+ ++ +D+ ++ P I + P+G + F+ AGG VLR L +A L+ H D T+ Sbjct: 292 GVDLELEDWTRIGRGTPTIVDLQPSGRYLMEEFYYAGGLPAVLRRLGEADLLPHKDALTV 351 Query: 269 LGQGLRQHCKEPMLAAEGENGRPGIVTWHDAPATSGDEGVLGTVAKPFAADGGLKLLQGN 328 GQ + + K DAP + DE V+ +AKP DGG+ +L+GN Sbjct: 352 NGQTMWDNVK-------------------DAPIYN-DE-VVRPLAKPLIEDGGICILRGN 390 Query: 329 LGR--AVIKISAVKPEHRVVEAPAVVFHSQEAFMAAFKAGELDRDFVAVITFQ--GPRAN 384 L AV+K SA PE AVVF + +LD D V+ + GP+ Sbjct: 391 LAPRGAVLKPSAATPELMKHRGRAVVFEDFNHYKERINDPDLDVDASCVLVMKNVGPKGY 450 Query: 385 -GMPELHSLTPALGILQDKGRHVALVTDGRMSG-ASGKVPAAIHVTPEVLGGGPLGRVRT 442 GM E+ ++ +L + + ++D RMSG A G V +HV PE GGPLG V+ Sbjct: 451 PGMAEVGNMGLPPKVLATGVKDMVRISDARMSGTAYGTV--ILHVAPEAAAGGPLGIVQD 508 Query: 443 GDIIRLDADAGTLQAKVEAAE 463 GD I LDA AG LQ + E Sbjct: 509 GDFIELDAYAGKLQLDISEEE 529 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 169 bits (427), Expect = 6e-43, Method: Compositional matrix adjust. Identities = 142/466 (30%), Positives = 215/466 (46%), Gaps = 57/466 (12%) Query: 38 NMFDAALYLGVCDKIVPGLLIGALHFGHLPAVFVPAGPMTSGMSNSDKV-------KIRQ 90 N D + L CDK P LL+GA +P + V GPM SG ++ ++ + Sbjct: 115 NPLDGVVLLMGCDKTTPALLMGAASV-DVPTIGVSGGPMLSGKYRGRELGSGTGVWQMSE 173 Query: 91 LYTAGKIGRAELLEAESKSYHGAGTCTFYGTANSNQMLMEAMGLHLPGAAFITPNTPLRD 150 AGK+ + E EAES + G C GTA++ ++EA+G+ LP A I R+ Sbjct: 174 DVRAGKMTQEEFFEAESCMHRSHGHCMTMGTASTMASMVEALGVGLPHNAAIPAVDARRN 233 Query: 151 ALTAAAAKQAAKITDLGSQYTPVGRMIDEKSIVNAIVALHATGGSTNHTLHLVAIAKAAG 210 L A + +I + + + +++ ++ NAI A GGSTN +HL+AIA G Sbjct: 234 VLARNAGR---RIVQMVEEDLVLSKILTRQAFENAIRVNAAIGGSTNAVIHLLAIAGRIG 290 Query: 211 IVIDWDDFDKLSAVVPLITKIYPNGTADVNHFHAAGGTGFVLRELIDAGLMHDDVTTILG 270 I +D D+D + +P + + P+G + F+ AGG V+R+L ++ T G Sbjct: 291 IKLDLKDWDDIGQQLPCLLDLQPSGKFLMEDFYYAGGLPAVIRQL--ESVIDKTALTANG 348 Query: 271 QGLRQHCKEPMLAAEGENGRPGIVTWHDAPATSGDEGVLGTVAKPFAADGGLKLLQGNLG 330 + L ++C+ DAP + +E V+ + KPF G+ +L+GNL Sbjct: 349 KTLWENCQ-------------------DAP--NWNEEVIRSFDKPFKEAAGIAILKGNLA 387 Query: 331 R--AVIKISAVKPEHRVVEAPAVVFHSQEAFMAAFKAGELDRDFVAVITFQ--GPRAN-G 385 AVIK SA P AVVF + + LD D V+ + GP+ G Sbjct: 388 PDGAVIKPSAATPALLKHRGRAVVFENSDDLHKRIDDENLDVDETCVLVLKNCGPKGYPG 447 Query: 386 MPELHSLTPALGILQDKGRHVALVTDGRMSG-ASGKVPAAIHVTPEVLGGGPLGRVRTGD 444 M E ++ IL+ + V+D RMSG A G V +HV+PE GGPL V+ GD Sbjct: 448 MAEAGNMPLPPKILRKGITDMVRVSDARMSGTAYGTV--VLHVSPEAAAGGPLAVVQNGD 505 Query: 445 IIRLDADAGTLQAKV-------EAAEWEAPPWDXGATFGLPPIFEK 483 I LD +A L V A+W+ P LPP ++ Sbjct: 506 FIELDVEARKLHLDVSDEELARRRAQWQKPE--------LPPQMQR 543 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 148 bits (373), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 141/489 (28%), Positives = 217/489 (44%), Gaps = 45/489 (9%) Query: 11 HGDVAVFARRFDAALYLGVCDKIVMSHNMFDAALYLGVCDKIVPGLLIGALHFGHLPAVF 70 HGD + L + +V +H DA + + CDKI PG+L+ A+ ++P VF Sbjct: 85 HGDARMLYSLPSRDLIADSVEYMVNAH-CADAMVCISNCDKITPGMLMAAMRL-NIPVVF 142 Query: 71 VPAGPMTSG----MSNSDKVKIR-----QLYTAG--KIGRAELLEAESKSYHGAGTCTFY 119 V GPM +G N+DK I+ + AG I A++ E E + G+C+ Sbjct: 143 VSGGPMEAGKVIKTVNTDKKVIKLDLVDAMIQAGDKNISDADVAEVERSACPTCGSCSGM 202 Query: 120 GTANSNQMLMEAMGLHLPGAAFITPNTPLRDALTAAAAK---QAAKITDLGSQYTPVGRM 176 TANS L E +GL LPG I R L A + + AK Y+ + R Sbjct: 203 FTANSMNCLTEVLGLSLPGNGTIVATHADRKELFLRAGRLIVELAKRHYEQDDYSILPRN 262 Query: 177 IDEKSI-VNAIVALHATGGSTNHTLHLVAIAKAAGIVIDWDDFDKLSAVVPLITKIYP-N 234 I K NA+ + GGSTN LHL+A A A + D D++S VP + K+ P Sbjct: 263 ICTKETWENAMTLDVSMGGSTNTVLHLLAAAHEAKVEFTMADIDRISRRVPCLCKVAPMT 322 Query: 235 GTADVNHFHAAGGTGFVLRELIDAGLMHDDVTTI----LGQGLRQH----CKEPML---- 282 + H AGG +L EL AGL+ T+ LG+ + + +P + Sbjct: 323 NKYHIEDVHRAGGIISILGELARAGLLDTSRPTVHSKTLGEAIEKFDIRVSSDPAVHKLF 382 Query: 283 -AAEGENGRPGIVTWHDAPATSGDE-----GVLGTVAKPFAADGGLKLLQGNLGR--AVI 334 AA G G P V + ++ G + ++ DGGL +L GN+ ++ Sbjct: 383 RAAPG--GVPTQVAFSQEERFEANDLDRANGCIRDREHAYSQDGGLAVLYGNIAEKGCIV 440 Query: 335 KISAVKPEHRVVEAPAVVFHSQEAFMAAFKAGELDRDFVAVITFQGPRANGMPELHSLTP 394 K + V A VF SQ+A + ++ V ++ ++GP+ G P + + Sbjct: 441 KTAGVDESILKFSGTARVFESQDAAVEGILGDKVKAGDVVIVRYEGPK--GGPGMQEMLY 498 Query: 395 ALGILQDK--GRHVALVTDGRMSGASGKVPAAIHVTPEVLGGGPLGRVRTGDIIRLDADA 452 ++ K G+ AL TDGR SG S + H +PE GG +G V GD+I +D Sbjct: 499 PTSYIKSKGLGKACALFTDGRFSGGSSGLVIG-HASPEAAEGGAIGLVEEGDMIDIDIPN 557 Query: 453 GTLQAKVEA 461 T+ ++ + Sbjct: 558 RTINLRISS 566 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 136 bits (343), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 127/473 (26%), Positives = 206/473 (43%), Gaps = 55/473 (11%) Query: 18 ARRFDAAL-----YLGVCDKIVMSHNMFDAALYLGVCDKIVPGLLIGALHFGHLPAVFVP 72 RR AA+ YLG+ + ++ DA + CDK P ++ A +PA+ + Sbjct: 96 CRRPTAAIDRNLAYLGLVE--ILHGYPIDAVVLTTGCDKTTPSQIMAAATV-DIPAIVLS 152 Query: 73 AGPMTSGMSNSDKV-------KIRQLYTAGKIGRAELLEAESKSYHGAGTCTFYGTANSN 125 GPM G + + V K R+L +AG I + LE + S +G C GTA++ Sbjct: 153 GGPMLDGWMDGELVGSGSAIWKGRKLLSAGSIDNEKFLEIAAASAPSSGHCNTMGTASTM 212 Query: 126 QMLMEAMGLHLPGAAFITPNTPLRDALTAAAAKQAAKITDLGSQYTPVGRMIDEKSIVNA 185 + EA+G+ L G + I P R+ A + +I + + ++ + ++A Sbjct: 213 NAMAEALGMSLTGCSAIP--APYRER-GQMAYETGRRIVGMAYEDLRPSAILTRDAFLDA 269 Query: 186 IVALHATGGSTNHTLHLVAIAKAAGIVIDWDDFDKLSAVVPLITKIYPNGTADVNHFHAA 245 IV A GGSTN H++A+A+ AG+ + +D+ K VPL+ + P G FH A Sbjct: 270 IVVNAAIGGSTNAQPHIMAMARHAGVELQSEDWMKYGYDVPLLLNMQPAGKYLGERFHRA 329 Query: 246 GGTGFVLRELIDAGLMHDDVTTILGQGLRQHCKEPMLAAEGENGRPGIVTWHDAPATSGD 305 GG ++ EL AG + + T G+ + AE GR S D Sbjct: 330 GGVPAIMWELQQAGKLRAERITATGKTM----------AENLQGR-----------ASND 368 Query: 306 EGVLGTVAKPFAADGGLKLLQGNL-GRAVIKISAVKPEHR-----------VVEAPAVVF 353 ++ A P G +L+GNL A++K S + R + E AVVF Sbjct: 369 REMIYPFAAPLRERAGFLVLKGNLFDFAIMKTSVISETFRERYLSTPGQENIFECRAVVF 428 Query: 354 HSQEAFMAAFKAGELDRDFVAVITFQGPRANGMP---ELHSLTPALGILQDKGRHVALVT 410 + + A L D ++ +G G P E+ ++ P +++ + + Sbjct: 429 DGSDDYHARINDPALKIDENTLLAIRGAGPVGWPGSAEVVNMQPPDALIKRGVSTLPTLG 488 Query: 411 DGRMSGASGKVPAAIHVTPEVLGGGPLGRVRTGDIIRLDADAGTLQAKVEAAE 463 DGR SG S P+ ++ +PE GG L +R GD +R+D + G V E Sbjct: 489 DGRQSGTSDS-PSILNASPESAVGGGLAYLRDGDRVRIDLNTGECNMLVSEEE 540 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,700,405 Number of extensions: 78732 Number of successful extensions: 235 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 214 Number of HSP's successfully gapped: 5 Length of query: 488 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 399 Effective length of database: 1,198,455 Effective search space: 478183545 Effective search space used: 478183545 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)