BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0122.p (545 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310432|ref|YP_003774524.1| glucose-6-phosphate isomerase [... 892 0.0 gi|300311812|ref|YP_003775904.1| glucose-6-phosphate isomerase [... 509 e-145 >gi|300310432|ref|YP_003774524.1| glucose-6-phosphate isomerase [Herbaspirillum seropedicae SmR1] Length = 554 Score = 892 bits (2306), Expect = 0.0, Method: Compositional matrix adjust. Identities = 443/567 (78%), Positives = 480/567 (84%), Gaps = 37/567 (6%) Query: 1 MHTTALTDTAAFQALQAHHATAVQWHLRDLFAQQPDRFERMHVRAAGLLLDYSKNRLNAD 60 MHTTALTDTAAF+ALQ HHA AV WH+R+LFAQQPDRFERMHVRAAGLLLDYSKNR+ ++ Sbjct: 1 MHTTALTDTAAFKALQHHHAKAVTWHMRELFAQQPDRFERMHVRAAGLLLDYSKNRIESE 60 Query: 61 TLSLLMSLARERGVETLREAMFSGEKINFTEQRAVLHTALRAPSDDRTAQVDGQPVAQDV 120 TL+LLMSLARERGVE LREAMF GEKINFTE RAVLHTALRAP+ AQVDGQPV+Q+V Sbjct: 61 TLALLMSLARERGVEALREAMFRGEKINFTEHRAVLHTALRAPAQACDAQVDGQPVSQEV 120 Query: 121 HAVLQHIRAFSERVRSGQWRGYTGREITDIVNIGIGGSFLGPQMVCTALRDYRHARLTAH 180 HAVL HIR FSERVRSG WRGYTGREITDIVNIGIGGSFLGPQMVCTALR+YRHARLT H Sbjct: 121 HAVLDHIRDFSERVRSGAWRGYTGREITDIVNIGIGGSFLGPQMVCTALRNYRHARLTTH 180 Query: 181 FVSNVDGHDLDEVLARIDPETTLFIVASKTFTTQETMLNAKSARAWFLQHGKSEDLAKHF 240 FVSNVDGHDLDEVL+R+DPETTLFIVASKTFTTQETMLNAKSARAWFL+HG+ DLA+HF Sbjct: 181 FVSNVDGHDLDEVLSRVDPETTLFIVASKTFTTQETMLNAKSARAWFLRHGQQADLARHF 240 Query: 241 VAVSTNAKDVSAFGIDTENMXFP-----------------------PPPNFAQFLAGAHA 277 VAVSTNA +V +FGID +NM FP +F+ FLAGAHA Sbjct: 241 VAVSTNAAEVESFGIDPDNM-FPFWDWVGGRYSVWSAIGLPIALLIGFEHFSAFLAGAHA 299 Query: 278 MDRHFREAPLESNMPVIQALVGIWNRNFLDSASLTIAPYHQWLRFFPAYLQQLDMESNGK 337 MDRHFR+APLE+NMPVIQAL+GIWNRNF +S SL++APYHQWLR+FPAYLQQLDMESNGK Sbjct: 300 MDRHFRDAPLEANMPVIQALIGIWNRNFFNSESLSVAPYHQWLRYFPAYLQQLDMESNGK 359 Query: 338 RITRDGTPVSVQTCPAIWGDVGTNGQHAYFQLLHQGTDITPIDFIAVLKPSHAIAEHQEA 397 RITRDGTPV V TCP IWGDVGTNGQHA+FQLLHQGT++TPIDFIAVLKPSH I EHQ+ Sbjct: 360 RITRDGTPVGVATCPFIWGDVGTNGQHAFFQLLHQGTNVTPIDFIAVLKPSHGIDEHQDV 419 Query: 398 LLANCFAQSEAFMRGKDADEVRADLQAQGVPEAELAALIPHKTFDGNRPSNTLLLPELSP 457 LLANCFAQSEAFMRGK ADEVRADL+ QGV AELAAL+PHKTFDGNRPSNTLLLPELSP Sbjct: 420 LLANCFAQSEAFMRGKQADEVRADLERQGVDAAELAALLPHKTFDGNRPSNTLLLPELSP 479 Query: 458 HALGAMIALYEHKTFVQGAIWGINSFDQWGVELGKVLAKTILAELKSGTAPVAARFXQDH 517 HALGAMIALYEHKTFVQGAIWGINSFDQWGVELGKVLAKTI EL+S P H Sbjct: 480 HALGAMIALYEHKTFVQGAIWGINSFDQWGVELGKVLAKTIHGELRSEPNPAL------H 533 Query: 518 PGRTEVRHRTGCALIALAREAARTGQQ 544 T ALIALAR+A + QQ Sbjct: 534 DASTN-------ALIALARQARQADQQ 553 >gi|300311812|ref|YP_003775904.1| glucose-6-phosphate isomerase [Herbaspirillum seropedicae SmR1] Length = 570 Score = 509 bits (1310), Expect = e-145, Method: Compositional matrix adjust. Identities = 263/529 (49%), Positives = 340/529 (64%), Gaps = 33/529 (6%) Query: 5 ALTDTAAFQALQAHHAT-AVQWHLRDLFAQQPDRFERMHVRAAGLLLDYSKNRLNADTLS 63 +LT +A+++LQ H+ A + L LFA R E + A G+ LDYSKN + ++T++ Sbjct: 21 SLTGKSAWRSLQEHYRDRARHFQLARLFAADDKRGESLTAEAVGIYLDYSKNLVTSETMA 80 Query: 64 LLMSLARERGVETLREAMFSGEKINFTEQRAVLHTALRAPSDDRTAQVDGQPVAQDVHAV 123 LLM LAR+ G+ EAMF G++IN TE R+VLHTALR P+ +R VDGQ V +V V Sbjct: 81 LLMQLARQCGLPDRIEAMFRGDRINLTENRSVLHTALRKPASERLV-VDGQDVVAEVQQV 139 Query: 124 LQHIRAFSERVRSGQWRGYTGREITDIVNIGIGGSFLGPQMVCTALRDYRHARLTAHFVS 183 L+ + F+ +VRSGQW G+TG I +VNIGIGGS LGP M ALR Y H LT HFVS Sbjct: 140 LRRMEGFARQVRSGQWLGHTGLPIRHVVNIGIGGSDLGPMMAYEALRPYSHDDLTLHFVS 199 Query: 184 NVDGHDLDEVLARIDPETTLFIVASKTFTTQETMLNAKSARAWFLQH-GKSEDLAKHFVA 242 +VDG + + + + E TLFIV SKTFTT ET+ NA +ARAW +Q G E +A+HFV Sbjct: 200 DVDGAAMLDTVRGLPAEQTLFIVCSKTFTTLETITNATAARAWLVQQLGAEEAVARHFVG 259 Query: 243 VSTNAKDVSAFGIDTENM----------------------XFPPPPNFAQFLAGAHAMDR 280 V+TN ++ FGID +NM P +F L G HAMD Sbjct: 260 VTTNIEEAQRFGIDADNMFGFWDWVGGRYSMTSAIGLSTMVAIGPEHFRAMLEGFHAMDE 319 Query: 281 HFREAPLESNMPVIQALVGIWNRNFLDSASLTIAPYHQWLRFFPAYLQQLDMESNGKRIT 340 HFR APLESN+PVI ++ +W NF +AS + PY Q L+ PAYLQQL MESNGK IT Sbjct: 320 HFRSAPLESNLPVILGMLTVWYTNFFGAASQAVLPYSQHLKRLPAYLQQLTMESNGKHIT 379 Query: 341 RDGTPVSVQTCPAIWGDVGTNGQHAYFQLLHQGTDITPIDFIAVLKPSHAIAEHQEALLA 400 +G PV QT P WGD G NGQH+++Q+LHQGT + P DFI + +P A+ + E L+A Sbjct: 380 VNGEPVDYQTSPVYWGDNGINGQHSFYQMLHQGTQLVPCDFIGLCQPQQALGQQHELLMA 439 Query: 401 NCFAQSEAFMRGKDADEVRADLQAQGVPEAELAALIPHKTFDGNRPSNTLLLPELSPHAL 460 N FAQSEA GKDAD+VR + G PE AL+PH+ F+GNRPSN++LL +L+P++L Sbjct: 440 NMFAQSEALAFGKDADQVRQE----GTPE----ALVPHRVFEGNRPSNSMLLQQLTPYSL 491 Query: 461 GAMIALYEHKTFVQGAIWGINSFDQWGVELGKVLAKTILAELKSGTAPV 509 G ++ALYEH F QG +W I+SFDQWGVELGK LAK EL P+ Sbjct: 492 GTLVALYEHSVFTQGVVWNIDSFDQWGVELGKQLAKRTARELIDHQQPL 540 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.133 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,736,713 Number of extensions: 74404 Number of successful extensions: 262 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 257 Number of HSP's successfully gapped: 3 Length of query: 545 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 455 Effective length of database: 1,193,720 Effective search space: 543142600 Effective search space used: 543142600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)