BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0112.p (259 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 146 2e-36 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 145 5e-36 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 142 2e-35 gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydroge... 134 7e-33 gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herb... 130 8e-32 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 146 bits (368), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 94/249 (37%), Positives = 139/249 (55%), Gaps = 3/249 (1%) Query: 10 MTRPHDFSGRVALVTGAAAGIGLATSQLLARCGARLVLVDRS-DAVLQLARQL-GPDHAH 67 M R +D RVALVTG A GIG + SQ LA G +++ D + DA + A L H Sbjct: 1 MNR-NDSGARVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRA 59 Query: 68 LGLVLDVCDEAAIGRAIVQVEQHYGRIDVLVNNAGVALLQEAESATCTDWDRTMAINLKA 127 L +D+ +I A +E+ YGR DVLVNNAGVA +W +TM +N+ Sbjct: 60 APLQMDLGSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTG 119 Query: 128 PFLLSQAVFPLMQRQQGGRIINLASQASVVALERHAAYCASKAGLVALTRVLALEWARHG 187 L Q LM +QQ GRI+N++S + + A AY SKA ++ LTR +A+E A++G Sbjct: 120 VLLAGQHAARLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYG 179 Query: 188 ITANAISPTVVETELGKQAWAGAVGVAMRARIPVGRFAQPDEVAHLVAYLASPQAAMITG 247 IT N+++P V+T + + + + +P+ R+ DE+A V YLA+ ++A ITG Sbjct: 180 ITVNSVAPGPVDTPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITG 239 Query: 248 ENILIDGGY 256 I +DGG+ Sbjct: 240 HVIPVDGGF 248 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 145 bits (365), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 93/246 (37%), Positives = 139/246 (56%), Gaps = 6/246 (2%) Query: 14 HDFSGRVALVTGAAAGIGLATSQLLARCGARLVLVDRSD----AVLQLARQLGPDHAHLG 69 + G+VALVTGA+ GIG A + LAR GA+++ S+ A+ + LGP+ A G Sbjct: 4 QNLQGQVALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPE-AGRG 62 Query: 70 LVLDVCDEAAIGRAIVQVEQHYGRIDVLVNNAGVALLQEAESATCTDWDRTMAINLKAPF 129 VL+V D A + QV++ +G + +LVNNAG+ Q A +WD +A NL A Sbjct: 63 AVLNVNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVG 122 Query: 130 LLSQAVFPLMQRQQGGRIINLASQASVVALERHAAYCASKAGLVALTRVLALEWARHGIT 189 LS+AV M + + GRIIN+ S Y A+KAG+ ++R LA E IT Sbjct: 123 RLSRAVLRGMMKARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNIT 182 Query: 190 ANAISPTVVETELGKQAWAGAVGVAMRARIPVGRFAQPDEVAHLVAYLASPQAAMITGEN 249 N ++P ++T++ +A A+ +IP+GR +E+A+ VA+LASPQAA ITG Sbjct: 183 VNCVAPGFIDTDM-TRALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGTT 241 Query: 250 ILIDGG 255 + ++GG Sbjct: 242 LNVNGG 247 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 142 bits (358), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 92/250 (36%), Positives = 142/250 (56%), Gaps = 17/250 (6%) Query: 19 RVALVTGAAA--GIGLATSQLLARCGARLVLVD--RSDAVLQLARQLGPDHAHLGLVLDV 74 +VA++TG A G+G +T++L+A GAR+V++D R++ AR LG H LGLV DV Sbjct: 6 KVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAAR-LGAQH--LGLVADV 62 Query: 75 CDEAAIGRAIVQVEQHYGRIDVLVNNAGVALLQEAESATCTDWDRTMAINLKAPFLLSQA 134 D+AA A V + +GRID+LVNNAGV + T D+DR + ++L+ +SQA Sbjct: 63 TDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGTLYMSQA 122 Query: 135 VFPLMQRQQGGRIINLASQASVVALERHAA------YCASKAGLVALTRVLALEWARHGI 188 V P MQ QQ G I+ ++S V+ +R Y A+KAG++ L R +A E+ GI Sbjct: 123 VLPAMQAQQSGSIVCISS----VSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQGI 178 Query: 189 TANAISPTVVETELGKQAWAGAVGVAMRARIPVGRFAQPDEVAHLVAYLASPQAAMITGE 248 N I+P ++ T++ K + + IP+GR PD+V + +LAS + TG Sbjct: 179 RVNCITPGLISTDINKDILSAEKKAEIAQTIPLGRLGGPDDVGGVCVFLASDLSQYCTGV 238 Query: 249 NILIDGGYTV 258 + ++GG + Sbjct: 239 TLDVNGGMLI 248 >gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 241 Score = 134 bits (337), Expect = 7e-33, Method: Compositional matrix adjust. Identities = 91/246 (36%), Positives = 139/246 (56%), Gaps = 13/246 (5%) Query: 15 DFSGRVALVTGAAAGIGLATSQLLARCGARLVLVDRSDAVL-QLARQLGPDHAHLGLVLD 73 F+G+ ++TGA GIG A + LLA+ GA+++ + R+ + L +L ++G + +D Sbjct: 2 SFAGKTVIITGAGRGIGRACALLLAQRGAQVIALARTASDLARLQEEIGARV----IAVD 57 Query: 74 VCDEAAIGRAIVQVEQHYGRIDVLVNNAGVALLQEAESATCTDWDRTMAINLKAPFLLSQ 133 + D AA + G D L+N+AG+ +L+ + ++ + +NL+A + Q Sbjct: 58 LSDPAAA----RRAMAEAGTADFLINSAGINVLESVLEMSDEGYEAVLGVNLRAALITCQ 113 Query: 134 AVFPLMQRQQGG--RIINLASQASVVALERHAAYCASKAGLVALTRVLALEWARHGITAN 191 A F M+ QGG ++N+ S A E+H Y ASKAGL TRVLA E HGI N Sbjct: 114 A-FARMRIAQGGGGAMVNITSIAGHRGFEQHLCYAASKAGLEGATRVLARELGPHGIRVN 172 Query: 192 AISPTVVETELGKQAWAG-AVGVAMRARIPVGRFAQPDEVAHLVAYLASPQAAMITGENI 250 A++PT+ TEL + AW+ A M AR P RFA P++VA +A L SP AAM+ G + Sbjct: 173 AVAPTITLTELAEAAWSDPAKSGPMMARHPSARFASPEDVARSIAMLLSPDAAMLNGAVV 232 Query: 251 LIDGGY 256 +DGG+ Sbjct: 233 PVDGGF 238 >gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 130 bits (328), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 83/245 (33%), Positives = 134/245 (54%), Gaps = 12/245 (4%) Query: 19 RVALVTGAAAGIGLATSQLLARCGARLVLV-----DRSDAVLQLARQLGPDHAHLGLVLD 73 +VA V G + GIG A Q LA GA + D++ A++ + G L + D Sbjct: 18 KVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVSSVQAKG--GRALAIQAD 75 Query: 74 VCDEAAIGRAIVQVEQHYGRIDVLVNNAGVALLQEAESATCTDWDRTMAINLKAPFLLSQ 133 D AA+ +AI + +GR+D+LVNNAGV + + D DRT+A+N+++ F+ +Q Sbjct: 76 SADAAALQQAIRLAAETFGRLDILVNNAGVLAMGPLDQFKLEDLDRTLAVNVRSVFVATQ 135 Query: 134 AVFPLMQRQQGGRIINLAS-QASVVALERHAAYCASKAGLVALTRVLALEWARHGITANA 192 M +GGR+I++ S A + A Y SK+ LV LT+ LA + GIT N Sbjct: 136 EAARYM--GEGGRVISIGSTNADRIPFAGGAVYAMSKSALVGLTKGLARDLGPRGITVNN 193 Query: 193 ISPTVVETELGKQAWAGAVGVAMRARIPVGRFAQPDEVAHLVAYLASPQAAMITGENILI 252 + P V+T++ +G ++ + + R+ + +E+A VAYLA P++ ITG N++I Sbjct: 194 VQPGPVDTDM--NPASGDFAASLTGLMALPRYGKAEEIASFVAYLAGPESGYITGANLMI 251 Query: 253 DGGYT 257 DGG++ Sbjct: 252 DGGFS 256 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.133 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 813,599 Number of extensions: 33348 Number of successful extensions: 306 Number of sequences better than 1.0e-08: 50 Number of HSP's gapped: 193 Number of HSP's successfully gapped: 50 Length of query: 259 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 176 Effective length of database: 1,226,865 Effective search space: 215928240 Effective search space used: 215928240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)