BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0104.p (173 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herb... 258 2e-70 gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspir... 83 1e-17 gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 62 3e-11 gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldeh... 60 9e-11 >gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 258 bits (660), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 117/129 (90%), Positives = 124/129 (96%) Query: 1 MQQGLVIHAPHDLRIQSLESAPLQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMV 60 MQQGLVIHAPHDLRIQ+L++ LQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMV Sbjct: 1 MQQGLVIHAPHDLRIQALDTGALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMV 60 Query: 61 LGHEVSGQIAEVGSAITDIAPGTRIAISPSRPCGVCKYCQEGKQNHCLDMRFYGSAMRTP 120 LGHEVSGQIAEVG+A+TDIAPGTRIAISPSRPCG CKYCQEGKQNHCLDMRFYGSAMRTP Sbjct: 61 LGHEVSGQIAEVGTAVTDIAPGTRIAISPSRPCGACKYCQEGKQNHCLDMRFYGSAMRTP 120 Query: 121 HVQGSVRKS 129 HVQG+ R+ Sbjct: 121 HVQGAFRQE 129 >gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 82.8 bits (203), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 47/129 (36%), Positives = 72/129 (55%), Gaps = 7/129 (5%) Query: 3 QGLVIHAPHDLRIQSLESAPLQ--PQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMV 60 Q LV+ A +L+++ ++ P Q Q +++++ GICGSDLHYY HG G +++ PMV Sbjct: 2 QALVLEATRELKLREID-LPQQMGAQDVRIRIHTVGICGSDLHYYTHGSIGPFKVEAPMV 60 Query: 61 LGHEVSGQIAEVGSAITDIAPGTRIAISPSRPCGVCKYCQEGKQNHCLDMRFYGSAMRTP 120 LGHE SG + EVGSA++ + G R+ + P P G N +RF+ TP Sbjct: 61 LGHEASGTVIEVGSAVSHLKVGDRVCMEPGIPRLDSPATLRGMYNLDPAVRFWA----TP 116 Query: 121 HVQGSVRKS 129 + G + S Sbjct: 117 PIHGCLTGS 125 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 61.6 bits (148), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 35/95 (36%), Positives = 50/95 (52%), Gaps = 4/95 (4%) Query: 13 LRIQSLESAPLQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVLGHEVSGQIAEV 72 L+I+ L+ P P ++ V++KA G+C SDL GV PM LGHE S ++ EV Sbjct: 23 LKIEELDLDPPGPGEVLVRMKAAGLCHSDLSVIT----GVRPRPVPMALGHEASAEVVEV 78 Query: 73 GSAITDIAPGTRIAISPSRPCGVCKYCQEGKQNHC 107 GS + D+ G + + CG C C EG+ C Sbjct: 79 GSGVNDLRAGDLVVLVFVPSCGHCLPCMEGRPALC 113 >gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 60.1 bits (144), Expect = 9e-11, Method: Compositional matrix adjust. Identities = 33/100 (33%), Positives = 52/100 (52%), Gaps = 4/100 (4%) Query: 3 QGLVIHAPHDLRIQSLESAPLQP-QQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVL 61 + L+ H+ HD+R++++ +Q + +KV A ICGSDLH Y + K +L Sbjct: 2 RALLYHSAHDVRVETVPDPIIQEADDVILKVTATAICGSDLHLYRGK---IPATKAGDIL 58 Query: 62 GHEVSGQIAEVGSAITDIAPGTRIAISPSRPCGVCKYCQE 101 GHE G + E G AI + G R+ + CG C +C + Sbjct: 59 GHEFMGVVVEAGPAIKRLKKGDRVVVPFVIACGQCFFCDQ 98 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.135 0.464 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 576,193 Number of extensions: 22082 Number of successful extensions: 75 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 74 Number of HSP's successfully gapped: 5 Length of query: 173 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 94 Effective length of database: 1,245,805 Effective search space: 117105670 Effective search space used: 117105670 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 127 (53.5 bits)