BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0096.p (440 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirill... 851 0.0 gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmemb... 100 4e-22 gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysi... 90 3e-19 gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter prote... 74 3e-14 >gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 851 bits (2198), Expect = 0.0, Method: Compositional matrix adjust. Identities = 414/440 (94%), Positives = 429/440 (97%) Query: 1 LENILLIVAAFFLVALNGFFVAAEFSLVKLRQTRIRAIAKTQGMRGRILAVVHNQLDAYL 60 +ENILLIV AFFLVALNGFFVAAEFSLVKLRQTRIRAIAKTQGMRGRILAVVHNQLDAYL Sbjct: 1 MENILLIVVAFFLVALNGFFVAAEFSLVKLRQTRIRAIAKTQGMRGRILAVVHNQLDAYL 60 Query: 61 SACQLGITLASLGLGWIGEPAFARLLEPLFSLVGVTNQELIHGISFVFAFFVISFLHIVA 120 SACQLGITLASLGLGWIGEPAFAR+LEPLFSL GVTNQELIHG+SFVFAFFVISFLHIVA Sbjct: 61 SACQLGITLASLGLGWIGEPAFARILEPLFSLAGVTNQELIHGVSFVFAFFVISFLHIVA 120 Query: 121 GELAPKSMAIRSPEKLGLWCAVPLYGFYWGMYPLIWVLNASSNWLLRVAGLGAGHGHDAH 180 GELAPKSMAIRSPEKLGLWCA+PLYGFYWGMYPLIWVLNASSNWLLRVAGLGAGHGHDAH Sbjct: 121 GELAPKSMAIRSPEKLGLWCAMPLYGFYWGMYPLIWVLNASSNWLLRVAGLGAGHGHDAH 180 Query: 181 YSSDELKLILRAGSKSGKSGKFTRDEWNVLAQSLNFAELDVADIMRPASEIVSLGDDKSL 240 YSSDELKLILRAG+KSGK+GKFTRDEWNVL QSLNFAELDVADIMRPASEIV+LGDDKSL Sbjct: 181 YSSDELKLILRAGNKSGKNGKFTRDEWNVLTQSLNFAELDVADIMRPASEIVALGDDKSL 240 Query: 241 EENLDIIYRNRYSRYPYFDAERQQVLGLVHLKDVFLAQQDGRAIGNLKEYLRPVQFISPA 300 EENLDIIYRNRYSRYPY+DAERQQVLGLVHLKDVFLAQQDGRAI NLK+YLRPVQ+ISPA Sbjct: 241 EENLDIIYRNRYSRYPYYDAERQQVLGLVHLKDVFLAQQDGRAIANLKDYLRPVQYISPA 300 Query: 301 LPALDLLRRFRTGMPHFAIIGKKGQPPQGFITLDNMLSFLVGEIRDEFRHNTGEWSRQDD 360 LPALDLLRRFRTG PHFA+IGKKGQPP GFITLDNMLS LVGEIRDEFRHNTGEW+RQDD Sbjct: 301 LPALDLLRRFRTGSPHFAVIGKKGQPPAGFITLDNMLSLLVGEIRDEFRHNTGEWTRQDD 360 Query: 361 GTLLGKGSLPIVTLEHMLGIDIEYDDSIDSVGGLVMEKLGDLPKEGQKIEFAAFDVVIKR 420 GTLLGKGSLPIVTLE+MLGIDIEYDDSIDSVGGLVMEKLGDLPKEGQKI+F AFDVVIKR Sbjct: 361 GTLLGKGSLPIVTLENMLGIDIEYDDSIDSVGGLVMEKLGDLPKEGQKIDFPAFDVVIKR 420 Query: 421 MSGPKIVLVKVYPKGGDVRE 440 MSGPKIVLVKVYPK + RE Sbjct: 421 MSGPKIVLVKVYPKLQEARE 440 >gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 434 Score = 99.8 bits (247), Expect = 4e-22, Method: Compositional matrix adjust. Identities = 95/436 (21%), Positives = 193/436 (44%), Gaps = 37/436 (8%) Query: 7 IVAAFFLVALNGFFVAAEFSLVKLRQTRIRAIAKTQGMRGRILAVVHNQLDAYLSACQLG 66 ++A F L+ L+ FF E +L+ + R+R +AK QG R I + L Sbjct: 10 VLALFILILLSAFFSMTETALMAANRHRLRHLAK-QGNRAAITIL-----------WLLD 57 Query: 67 ITLASLGLGWIGEPAFARLLEPLFSLVGV----TNQELIHGISFVFAFFVISFLHIVAGE 122 T L L I A L L + + + +Q +I + A +I F E Sbjct: 58 RTERLLSLVLIANTAINALATALVTALAIAAFGNHQRVITAATACVAALLIIF-----AE 112 Query: 123 LAPKSMAIRSPEKLGLWCAVPLYGFYWGMYPLIWVLNASSNWLLRVAGLGAG-HGHDAHY 181 + PK + PE++ L+ L PLIW +N + +LR+ + G + Sbjct: 113 IVPKVIGATYPERISLFTGFILKPLMRVAKPLIWFVNLFVSAILRLLRIKTGARAAEQRI 172 Query: 182 SSDELK-LILRAGSKSGKSGKFTRDEWNVLAQSLNFAELDVADIMRPASEIVSLGDDKSL 240 S +EL+ ++L GS + K ++L + ++ V D+M P S++ +L + Sbjct: 173 SPEELRSIVLEGGSFIPQKHK------SILLNLFDLEKISVEDVMTPRSQVEALNLAAPV 226 Query: 241 EENLDIIYRNRYSRYPYFDAERQQVLGLVHLKDV--FLAQQDGRAIGNLKEYLRPVQFIS 298 ++ L + +++ P F+ E ++G++H++ +++ + +++E L ++ Sbjct: 227 DDILHQLATCYHNKLPVFEGEINHIVGILHVRKTVSLFRREEEISHDDIRELLSAPYYVP 286 Query: 299 PALPALDLLRRFRTGMPHFAIIGKKGQPPQGFITLDNMLSFLVGEIRDEFRHNTGE---W 355 L+ F+ ++ + QG +TL++++ ++GE ++GE W Sbjct: 287 VETDVFTQLQYFQENHERLGVVVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGSSGEGFGW 346 Query: 356 SRQDDGTLLGKGSLPIVTLEHMLGIDIEYDDSIDSVGGLVMEKLGDLPKEGQKIEFAAFD 415 + DG L +GS + + LG+ + D ++ GL++E L D+P+ ++ Sbjct: 347 DK--DGACLLEGSTTLRDINKRLGLQLPLDGP-KTINGLLLEYLQDIPEASVAVKMDGCV 403 Query: 416 VVIKRMSGPKIVLVKV 431 + I ++ I +VK+ Sbjct: 404 IEIVQVQNQAIRMVKL 419 >gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 90.1 bits (222), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 93/416 (22%), Positives = 180/416 (43%), Gaps = 18/416 (4%) Query: 17 NGFFVAAEFSLVKLRQTRIRAIAKTQGMRG--RILAVVHNQLDAYLSACQLGITLASLGL 74 + F AE S+ R+ R+ ++K +G+ R+L++ Q + + Q+G+ ++ Sbjct: 17 SAFLSCAEISIAASRKIRLEMMSK-EGVHNADRVLSL-QAQPGNFFTVVQIGLNAVAILG 74 Query: 75 GWIGEPAFARLLEPLFSLVGVTNQELIHGISFVFAFFVISFLHIVAGELAPKSMAIRSPE 134 G +GEPA + + S + + SF +F V++ I+ +L PK +A+ +PE Sbjct: 75 GVVGEPALTPHMREIISSFYIG--PWLENASFAMSFLVVTAFFILFADLMPKRLAMVAPE 132 Query: 135 KLGLWCAVPLYGFYWGMYPLIWVLNASSNWLLRVAGLGAGHGHDAHYSSDELKLILRAGS 194 + + P+ PL+W N+ S W+ + GL + +E+ ++ AG+ Sbjct: 133 RAAVALVKPMLLLETLFKPLVWFFNSLSKWIFTLFGL--PQVRQEVITPNEIHAMVDAGA 190 Query: 195 KSGKSGKFTRDEWNVLAQSLNFAELDVADIMRPASEIVSLGDDKSLEENLDIIYRNRYSR 254 ++G E +++ V M P IV S I + +SR Sbjct: 191 ---QAGVLLTQEHHLIENVFELESRYVPSAMTPRDSIVYFLLTDSEATIRQKIIDHPHSR 247 Query: 255 YPYFDAERQQVLGLVHLKDVF--LAQQDGRAIGNLKEYLRPVQFISPALPALDLLRRFRT 312 + D VLG + KDV L + + ++ + K + P I +L ++L R + Sbjct: 248 FLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLAD-KGLVHPPLSIPDSLNLWEVLERMKA 306 Query: 313 GMPHFAIIGKKGQPPQGFITLDNMLSFLVGEIRDEFRHNTGEWSRQDDGTLLGKGSLPIV 372 AI+ + G I+ ++ L+G + + R+D+ + L G P+ Sbjct: 307 MDEDLAIVVNEYALVVGIISTTDVTGVLMGNLL-AITEEEQQIVRRDENSWLMDGLTPLN 365 Query: 373 TLEHMLGIDIEYDDSI--DSVGGLVMEKLGDLPKEGQKIEFAAFDVVIKRMSGPKI 426 + L ID EY + + +++ G +M L +PK+ + FA F + + G +I Sbjct: 366 DVMSELDID-EYPNPVGYETLSGFIMYMLRKIPKKTDFVLFAEFKFEVVDIEGNRI 420 >gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter protein [Herbaspirillum seropedicae SmR1] Length = 292 Score = 73.6 bits (179), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 61/238 (25%), Positives = 117/238 (49%), Gaps = 13/238 (5%) Query: 205 DEWNVLAQSLNFAELDVADIMRPASEIVSLGDDKSLEENLDIIYRNRYSRYPYFDAERQQ 264 D +++ ++L V DIM P S++ ++ K +E + + +SR+P D ER + Sbjct: 52 DALSMIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVLATSHSRFPAVDGERDK 111 Query: 265 VLGLVHLKDV--FLAQQDGRAIGNLKEYLRPVQFISPALPALDLLRRFRTGMPHFAIIGK 322 V+G++ KD+ + A++ ++++ LRP FI + LLR FR H A++ Sbjct: 112 VVGILLAKDLLRYYAEESF----DVRDMLRPAIFIPESKRLNVLLRDFRANRNHMAVVVD 167 Query: 323 KGQPPQGFITLDNMLSFLVGEIRDEFRHNTGEWS--RQDDGTLLGKGSLPIVTLEHMLGI 380 + G IT++++L +VG+I DE+ + E + DG + + +T Sbjct: 168 EYGGVAGLITIEDVLEQIVGDIEDEYDFDEAEDNVLALKDGPHGARWRVKALTEIEQFNE 227 Query: 381 DIE---YDDSIDSVGGLVMEKLGDLPKEGQKIEFAA--FDVVIKRMSGPKIVLVKVYP 433 ++ DD D++GGLV +G +P++G + + F+V+ ++LV P Sbjct: 228 EVGSRFSDDDADTIGGLVANHVGRVPRKGDEFDIDGLHFEVLRADARQVHVLLVSQLP 285 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.142 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,333,419 Number of extensions: 57558 Number of successful extensions: 151 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 144 Number of HSP's successfully gapped: 4 Length of query: 440 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 352 Effective length of database: 1,203,190 Effective search space: 423522880 Effective search space used: 423522880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)