BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0095.p (335 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310417|ref|YP_003774509.1| zinc-containing alcohol dehydro... 593 e-171 gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [H... 83 4e-17 gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [H... 73 3e-14 gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [H... 72 5e-14 gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [H... 72 5e-14 >gi|300310417|ref|YP_003774509.1| zinc-containing alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 593 bits (1528), Expect = e-171, Method: Compositional matrix adjust. Identities = 294/333 (88%), Positives = 308/333 (92%) Query: 1 MKAIITNPQAKAIYDAASLADTTLPDPVAGEHDLLVEVKAISVNPVDTKIRASAGEARVL 60 MKAI+T PQ+ I +L +TTLPDP GEHDLLVEVKAISVNPVDTKIRASA EARVL Sbjct: 1 MKAILTQPQSPLISAPDALIETTLPDPTPGEHDLLVEVKAISVNPVDTKIRASAKEARVL 60 Query: 61 GWDVSGVVRAVGPAVSLFAPGDEVFYAGSIVRPGANSELHVVDERIVGRKPASLGHADAA 120 GWDVSGVVRAVGPAV+LFAPGDEVFYAGSI RPGANSELHVVDERIVGRKP+SLGHADAA Sbjct: 61 GWDVSGVVRAVGPAVTLFAPGDEVFYAGSITRPGANSELHVVDERIVGRKPSSLGHADAA 120 Query: 121 AIPLTAITAWELLFDRIGVAEGAGQGKRLLIIGAAGGVGSILTQLARKLTQLTVIGTASR 180 AIPLTAITAWELLFDR+ VAEG GQG+ LLIIGAAGGVGS+LTQLARKLTQLTVIGTASR Sbjct: 121 AIPLTAITAWELLFDRVAVAEGGGQGQHLLIIGAAGGVGSMLTQLARKLTQLTVIGTASR 180 Query: 181 PETAQWVSELGAHHVIDHSQPFKPQLEQLGFPEVDIVISLTHTDQHYAQIIEALAPQGQL 240 PETA WV ELGAHHVIDHSQP KPQLE+LG PEV IVISLTHTDQHYAQII+ LAPQGQL Sbjct: 181 PETAHWVGELGAHHVIDHSQPLKPQLEKLGIPEVGIVISLTHTDQHYAQIIDVLAPQGQL 240 Query: 241 ALIDDPATLDAVPLKRKSISLHWELMFTRSMFQTADMIRQHELLQRVSQLLDEGVLRTTT 300 ALIDDPA+LDA+PLKRKSISLHWELMFTRSMFQT DMIRQHELLQRVSQLLD+GVLRTTT Sbjct: 241 ALIDDPASLDAMPLKRKSISLHWELMFTRSMFQTPDMIRQHELLQRVSQLLDQGVLRTTT 300 Query: 301 GEHYGRISAENLRRAHALLESGKAKGKIVLEGF 333 GEHYGRI A NLRRAH LLESGKAKGKIVLEGF Sbjct: 301 GEHYGRIDAANLRRAHGLLESGKAKGKIVLEGF 333 >gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 324 Score = 82.8 bits (203), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 95/320 (29%), Positives = 143/320 (44%), Gaps = 27/320 (8%) Query: 21 DTTLPDPVAGEHDLLVEVKAISVNPVDTKIRAS-------AGEARVLGWDVSGVVRAVGP 73 D + +P AGE + + A+ +N +D RA AG LG + +G + AVG Sbjct: 20 DVEVGEPGAGE--VRIRHAAVGLNFIDCYFRAGLYPQPLPAG----LGQEAAGTIEAVGA 73 Query: 74 AVSLFAPGDEVFYAGSIVRP-GANSELHVVDERIVGRKPASLGHADAAAIPLTAITAWEL 132 VS F GD V YAG RP GA +E V+ I+ + P ++ AAA+ L +T L Sbjct: 74 GVSGFKVGDRVAYAG---RPNGAYAEERVMPADILVKLPDAISFDTAAAMMLQGMTVQYL 130 Query: 133 LFDRIGVAEGAGQGKRLLIIGAAGGVGSILTQLARKLTQLTVIGTASRPETAQWVSELGA 192 L V G +L AAGG+G I Q AR L + +IGT S E A + GA Sbjct: 131 LRQTYAVQP----GDTILFHAAAGGIGLIACQWARALG-VNLIGTVSSDEKAALALQHGA 185 Query: 193 HHVIDH-SQPFKPQLEQL-GFPEVDIVISLTHTDQHYAQIIEALAPQGQLALIDDPATLD 250 HVI++ ++ F ++++L G V +V D ++ L P+G + + A+ Sbjct: 186 AHVINYKTENFVERVKELTGGRGVQVVYDSIGKDTFLGS-LDCLRPRGMMVSFGN-ASGP 243 Query: 251 AVPLKRKSISLHWELMFTRSMFQTADMIRQHELLQRVSQLLDEGVLRTTTGEHYGRISAE 310 P ++ L TR R EL L D + + E R + + Sbjct: 244 VAPFGVSELASRGSLFLTRPSLGNYTSTRA-ELDATAGDLFDMVLGNKISIEINQRYALK 302 Query: 311 NLRRAHALLESGKAKGKIVL 330 ++ +AHA LE K G +L Sbjct: 303 DVAQAHADLEGRKTTGSTIL 322 >gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 72.8 bits (177), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 91/326 (27%), Positives = 152/326 (46%), Gaps = 33/326 (10%) Query: 20 ADTTLPDPVAGEHDLLVEVKAISVNPVDTKIRAS-----AGEARVLGWDVSGVVRAVGPA 74 + +P+ AGE +L++V A VN D R G + + G +V+G + A Sbjct: 18 CERPVPELKAGE--VLIKVHAAGVNRPDVFQRTGNYPVPPGASDLPGLEVAGEIVAGDLG 75 Query: 75 VSLFAPGDEVFYAGSIVRPGANSELHVVDERIVGRKPASLGHADAAAIPLTAITAWELLF 134 S F GD V ++V+ G +E P L +AAA+P T W +F Sbjct: 76 DSGFKLGDLVC---ALVQGGGYAEFCAAPLAQCLPVPKGLSALEAAALPENYFTVWSNVF 132 Query: 135 DRIGVAEGAGQGKRLLIIGAAGGVGSILTQLARKLTQLTVIGTASRPETAQWVSELGAHH 194 DR + +G+ + LL+ G + G+G Q+AR L V TA E + ELGA Sbjct: 133 DR---GQLSGE-ETLLVQGGSSGIGVTAIQIARALGH-RVFATAGSAEKCRACEELGAER 187 Query: 195 VIDH-SQPFKPQLEQL-GFPEVDIVISLTHTDQHYAQIIEALAPQGQLALIDD----PAT 248 I++ ++ F+ +++L G VD+++ + D + + I+ALA G+L I Sbjct: 188 AINYKTEDFEAVVKELTGGRGVDVILDMVGGD-YLPREIKALADDGRLVFIAQLGGAKGQ 246 Query: 249 LDAVPLKRKSISLHWELMFTRSM-FQT--ADMIRQHELLQRVSQLLDEGVLRTTTGEHYG 305 LD + R+ +++ + R + F+ A +R+H V L++ G +R H+ Sbjct: 247 LDMGQVMRRRLTITGSTLRPRPVAFKAAIAAKLREH-----VWPLIEAGKIRPVI--HH- 298 Query: 306 RISAENLRRAHALLESGKAKGKIVLE 331 R E +AHA++E GKI+LE Sbjct: 299 RFPLEQAAQAHAMMEGSTHIGKIMLE 324 >gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 309 Score = 72.4 bits (176), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 88/306 (28%), Positives = 143/306 (46%), Gaps = 40/306 (13%) Query: 36 VEVKAISVNPVDTKIRASAGE-------ARVLGWDVSGVVRAVGPAVSLFAPGDEVFYAG 88 V ++A V PVDTK+RA + +V G D +GV+ A+G V G+ V Sbjct: 32 VAMQAAGVAPVDTKLRAGRLQHLVALDLPKVPGRDGAGVITALGEGVHTLQVGEAVCVMP 91 Query: 89 SIVRPGAN-SELHVVDERIVGRKPASLGHADAAAIPLTAITAWELLFDRIGVAEGAGQGK 147 S+ G +EL + E++V RKP L AAA+ A +AW L +A G Sbjct: 92 SMAGRGTTLAELVLPQEQVV-RKPDVLSMQQAAALLQPACSAWIALMQTAPIAA----GM 146 Query: 148 RLLIIGAAGGVGSILTQLARKLTQLTVIGTASRPETAQWVSELGAHHVIDHSQPFKPQLE 207 ++ I +G VGS++ QLA L + + R ++V+ LGA V+ + + + Sbjct: 147 KVFIHAGSGAVGSLMVQLAAHLG--AEVTASCRSSNVEYVAALGASRVLAYDR------D 198 Query: 208 QLG-FPEVDIVISLTHTDQHYAQIIEALAPQGQLALIDDPATLDAVPLKRKSISLHWELM 266 +LG + D+VI L H Q L GQL L A P++ + Sbjct: 199 ELGRVRDQDVVIDLVGGAAH-DQSYRMLRRGGQLVW------LVAAPIRERGEE------ 245 Query: 267 FTRSMFQTADMIRQHELLQRVSQLLDEGVLRTTTGEHYGRISAENLRRAHALLESGK-AK 325 F S+ + A ++ +LQ+V+ L +GV R G + + + RAH +E+G+ + Sbjct: 246 FGVSV-RRALIVEDQSILQQVAALAQQGVWRPAVS---GVLPMQEIARAHQRIEAGEVTR 301 Query: 326 GKIVLE 331 G++VL+ Sbjct: 302 GRLVLD 307 >gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 72.4 bits (176), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 94/340 (27%), Positives = 144/340 (42%), Gaps = 57/340 (16%) Query: 21 DTTLPDPVAGEHDLLVEVKAISVNPVDTKIR-----------ASAGEARVLGWDVSGVVR 69 D +P+ GE +LV V A+ +NP D +R + +LG D+SGVV Sbjct: 24 DAPVPELQPGE--VLVRVHAVGLNPPDWYLREGYKMLPPEWQPTVAFPLILGTDISGVVE 81 Query: 70 AVGPAVSLFAPGDEVFYAGSIVR--------PGANSELHVVDERIVGRKPASLGHADAAA 121 AV V FA GDEV+ S+VR A ++ V + KP + HA AAA Sbjct: 82 AVADDVKDFAVGDEVY---SMVRFPSGLAGGSRAYAQYVSVAASEIALKPQRISHAQAAA 138 Query: 122 IPLTAITAWELLFDRIGVAEGAGQGKR----------LLIIGAAGGVGSILTQLAR-KLT 170 P++ +TAW+ + + + Q R +++ GAAGGVG QLA+ K Sbjct: 139 APMSLLTAWQFMIELGHDIQNPLQPHRHEPVPLKDKTVVVNGAAGGVGHFALQLAKWKGA 198 Query: 171 QLTVIGTASRPETAQWVSELGAHHVIDHSQPFKPQLEQLGFPEVDIVISLTHTDQHYAQI 230 ++ IG+ ++ +LGA ID+++ + +VD+VI + Sbjct: 199 RVIAIGSGGH---EAFLRDLGADEFIDYTRSAAEDV----LSDVDLVIDAVGGPAS-GRF 250 Query: 231 IEALAPQGQLALIDDPATLDAVPLKRKSISLHWELMFTRSMFQTADMIRQHELLQRVSQL 290 + L G L I PL L S Q Q LQ+++ L Sbjct: 251 LRTLKRGGALFPI--------FPLGFSGAEEAARLGVNVSTTQVRSSGAQ---LQQLAGL 299 Query: 291 LDEGVLRTTTGEHYGRISAENLRRAHALLESGKAKGKIVL 330 LD GV+R + + ++ +AH G +GKIVL Sbjct: 300 LDSGVVRVAIDSTF---ALQDAGKAHERAAQGHIRGKIVL 336 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,124,042 Number of extensions: 50516 Number of successful extensions: 227 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 217 Number of HSP's successfully gapped: 5 Length of query: 335 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 249 Effective length of database: 1,212,660 Effective search space: 301952340 Effective search space used: 301952340 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)