BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0087.p (524 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 887 0.0 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 456 e-129 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 369 e-103 gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 335 4e-93 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 297 1e-81 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 887 bits (2292), Expect = 0.0, Method: Compositional matrix adjust. Identities = 431/463 (93%), Positives = 447/463 (96%) Query: 2 LQQLKDPSLLRQQAYLNGQWCDADQGGKHEVYNPANGSLLGSVPMMGAAETRRAIEAANA 61 + QLKDP+LLRQQA++NGQWCDADQG + V+NPANG LLG VP+MGAAETRRAIEAANA Sbjct: 1 MLQLKDPALLRQQAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANA 60 Query: 62 AWPAWKKKTAKERSVILRRWYELMMANADDLALIMTAEQGKPLAEAKGEIGYAASFIEWF 121 AWPAWKKKTAKERS ILRRWYELM+AN DDLALIMTAEQGKPLAEA+GEIGYAASFIEWF Sbjct: 61 AWPAWKKKTAKERSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWF 120 Query: 122 AEEGKRTYGDTIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVL 181 AEEGKRTYGDTIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVL Sbjct: 121 AEEGKRTYGDTIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVL 180 Query: 182 KPAEATPFSALALAVLAERAGIPAGVFSVVTGSAKDIGGEMTSNPIVRKISFTGSTGVGK 241 KPAEATPFSALALAVLAERAGIPAGVFSVVTG+ K IGGEMTSNPIVRKISFTGSTGVGK Sbjct: 181 KPAEATPFSALALAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGK 240 Query: 242 LLMEQSASSIKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRLYVQD 301 LLM+QSASSIKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANR+YVQD Sbjct: 241 LLMQQSASSIKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQD 300 Query: 302 GVYDAFAAKLVEAVKKLKVGDGMENGVTQGPLINEQAVKKVEQHIADAVSKGARVLLGGK 361 GVYDAFAAKLVEAVKK KVG G E GVTQGPLINEQAV+KVEQH+ADAV+KGARVLLGGK Sbjct: 301 GVYDAFAAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGK 360 Query: 362 RHPLGHSFFEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEAVALANDTEFGLASYFY 421 RH LGHSFFEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEE +ALANDTEFGLASYFY Sbjct: 361 RHALGHSFFEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFY 420 Query: 422 SRDIGRIWRVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGR 464 SRDIGRIWRVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGR Sbjct: 421 SRDIGRIWRVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGR 463 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 456 bits (1173), Expect = e-129, Method: Compositional matrix adjust. Identities = 253/467 (54%), Positives = 333/467 (71%), Gaps = 7/467 (1%) Query: 5 LKDPSLLRQQAYLNGQWCDADQGGKHEVYNPANGSLLGSVPMMGAAETRRAIEAANAAWP 64 L+ L+ + W A G + +V +PA G + SVP GAA+ R A+EAA AA+ Sbjct: 6 LQRQDLMPGAQLIGADWRGAADGRQLDVSDPATGQVFASVPDGGAADARAAVEAAVAAFA 65 Query: 65 AWKKKTAKERSVILRRWYELMMANADDLALIMTAEQGKPLAEAKGEIGYAASFIEWFAEE 124 AW+ AK+R+ I++RW +L++A+ DDL +++ EQGKPLAEAKGE+ YAAS++EWF EE Sbjct: 66 AWRATPAKQRAGIIKRWNDLLLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEE 125 Query: 125 GKRTYGDTIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPA 184 R GD IP+P R++ +KE +GV AAITPWNFPAAMI RK PALAAGC +V KPA Sbjct: 126 ATRANGDIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPA 185 Query: 185 EATPFSALALAVLAERAGIPAGVFSVVTGSAK---DIGGEMTSNPIVRKISFTGSTGVGK 241 E TP ++LAL LA+ AG+P GV ++VT S + ++ ++ VRKISFTGST VGK Sbjct: 186 EDTPLTSLALVRLAQEAGVPVGVINIVTASRERTPEVVDVWLADGRVRKISFTGSTAVGK 245 Query: 242 LLMEQSASSIKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRLYVQD 301 L SA ++KKLSLELGGNAPFIVFDDAD+DAA++G +A+K+RN GQTCV NR+YVQ+ Sbjct: 246 HLARHSADTLKKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNGGQTCVSPNRIYVQE 305 Query: 302 GVYDAFAAKLVEAVKKLKVGDGMENGVTQGPLINEQAVKKVEQHIADAVSKGARVLLGGK 361 VYDAF KL V LKVG + GP+IN +A+ K++QH+ DA+++GARV+ GGK Sbjct: 306 KVYDAFVDKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIARGARVITGGK 365 Query: 362 RHP----LGHSFFEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEAVALANDTEFGLA 417 R +++ PTVLADVT AMQ + EETFGP+AP+ RF T++E +A AN T FGLA Sbjct: 366 RLQGPGFGSDNYYAPTVLADVTGAMQCSCEETFGPVAPITRFATEDEVIAAANATPFGLA 425 Query: 418 SYFYSRDIGRIWRVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGR 464 +YFYS D+ RI RV + LESG+VG+N G ++ E APFGGVK+SG GR Sbjct: 426 AYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGR 472 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 369 bits (946), Expect = e-103, Method: Compositional matrix adjust. Identities = 199/459 (43%), Positives = 285/459 (62%), Gaps = 2/459 (0%) Query: 14 QAYLNGQWCDADQGGKHEVYNPANGSLLGSVPMMGAAETRRAIEAANAAWPAWKKKTAKE 73 Q ++NG+W + G +V NPA ++G + A+ RA+EAA+ + W+ +A E Sbjct: 6 QLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFE 65 Query: 74 RSVILRRWYELMMANADDLALIMTAEQGKPLAEAKGEIGYAASFIEWFAEEGKRTYGDTI 133 RS I+RR +L+ A+++A +MT EQGKPLAEAK E AA I+WFAEE +RTYG + Sbjct: 66 RSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLV 125 Query: 134 PSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALA 193 P+ P +VIKE +G AA TPWNFP + RK ALAAGC +++K E TP S Sbjct: 126 PARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAE 185 Query: 194 LAVLAERAGIPAGVFSVVTGSAKDIGGEMTSNPIVRKISFTGSTGVGKLLMEQSASSIKK 253 L AG+PAGV ++V G +I + +P+++KISFTGST VGK L + +K+ Sbjct: 186 LIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMKR 245 Query: 254 LSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRLYVQDGVYDAFAAKLVE 313 ++ELGG+AP +VFDDAD+D A + +K+RNAGQ CV R VQ GV++AF K V Sbjct: 246 ATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFVT 305 Query: 314 AVKKLKVGDGMENGVTQGPLINEQAVKKVEQHIADAVSKGARVLLGGKRHPLGHSFFEPT 373 ++LKVG+G+E G+T GP+ N++ + +E I DAVS+GA++ GGKR FF+PT Sbjct: 306 YAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGYFFQPT 365 Query: 374 VLADVTPAMQVAREETFGPMAPLFRFKTDEEAVALANDTEFGLASYFYSRDIGRIWRVAE 433 VL ++ + EE FGP+A + F+T +E + AN +GLA+Y +++ +A Sbjct: 366 VLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTKHALAA 425 Query: 434 GLESGMVGIN-TGLISNEVAPFGGVKQSGLGRXVGTNFV 471 +ESGM+ IN GL E+ PFGG+K SG G GT + Sbjct: 426 RVESGMLTINHLGLALPEL-PFGGIKDSGYGSEGGTEAI 463 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 335 bits (860), Expect = 4e-93, Method: Compositional matrix adjust. Identities = 184/444 (41%), Positives = 275/444 (61%) Query: 14 QAYLNGQWCDADQGGKHEVYNPANGSLLGSVPMMGAAETRRAIEAANAAWPAWKKKTAKE 73 Q Y++G++ + EV +PA G +LG +P A+ AI+AA+ A+ W++ + E Sbjct: 6 QLYIDGRFHGVEGRRYQEVRDPATGDVLGRLPWAERADIEEAIQAAHRAFQQWRQVSPLE 65 Query: 74 RSVILRRWYELMMANADDLALIMTAEQGKPLAEAKGEIGYAASFIEWFAEEGKRTYGDTI 133 RS ILRR L A +A +T + GKPLA+A EI AA ++W AEEG+R YG + Sbjct: 66 RSDILRRAAALARERAQAMAPAITMDNGKPLADAVAEIINAAEHLDWHAEEGRRLYGRVV 125 Query: 134 PSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALA 193 + P R +V++E +GVCAAI+PWNFP +K A+AAGC +VLK +P + + Sbjct: 126 GARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESPAAIVL 185 Query: 194 LAVLAERAGIPAGVFSVVTGSAKDIGGEMTSNPIVRKISFTGSTGVGKLLMEQSASSIKK 253 +A L AG+PAGVF++V+G + +I + +P+VR ISFTGS VG L Q+A +K+ Sbjct: 186 MAQLFHDAGVPAGVFNIVSGVSSEISQHLIESPLVRNISFTGSVPVGSKLAAQAALHMKR 245 Query: 254 LSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRLYVQDGVYDAFAAKLVE 313 +S+ELGG+AP +VFDDA+++ A K RNAGQ CV +R YVQ G+Y F E Sbjct: 246 MSMELGGHAPVLVFDDAEVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIYARFVTAFAE 305 Query: 314 AVKKLKVGDGMENGVTQGPLINEQAVKKVEQHIADAVSKGARVLLGGKRHPLGHSFFEPT 373 A+ ++KVG G E GV GPLI+ + + V+ I DA+++G+ ++ GG R F+ PT Sbjct: 306 ALLQIKVGPGHEPGVQMGPLIHGRRFEAVQGLIDDALAQGSELVTGGARLGQRGHFYAPT 365 Query: 374 VLADVTPAMQVAREETFGPMAPLFRFKTDEEAVALANDTEFGLASYFYSRDIGRIWRVAE 433 ++ V P+ +V +EE FGP+A + F EEA+A+AN GLASY +++ + RV+ Sbjct: 366 LITGVPPSARVMQEEPFGPIAAVTPFSQAEEAIAMANALPLGLASYVFTQSLDTAHRVSR 425 Query: 434 GLESGMVGINTGLISNEVAPFGGV 457 L++GMV IN +++ PFGG+ Sbjct: 426 DLQAGMVNINHSGMAHPELPFGGI 449 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 297 bits (760), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 171/460 (37%), Positives = 255/460 (55%), Gaps = 14/460 (3%) Query: 16 YLNGQWCDADQGGKHEVYNPANGSLLGSVPMMGAAETRRAIEAANAAWPAWKKKTAKERS 75 Y++G+W + V PA + + + AA+ +A++AA A PAW + ER+ Sbjct: 19 YIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIPAWAATSPLERA 78 Query: 76 VILRRWYELMMANADDLALIMTAEQGKPLAEAKGEIGYAASFIEWFAEEGKRTYGDTI-- 133 +L R + L++ ++ A +T E G P I YA + A E R D + Sbjct: 79 ALLGRVHALLLERSELFAQALTLEMGAP-------ISYARTAHVPLAAEHLRVARDNLAD 131 Query: 134 -PSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSAL 192 P P +++E IGVCA ITPWN+P IT K GPALAAGC +VLKP+E +P SAL Sbjct: 132 YPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSAL 191 Query: 193 ALAVLAERAGIPAGVFSVVTGSAKDIGGEMTSNPIVRKISFTGSTGVGKLLMEQSASSIK 252 A + AG+PAGVF++V GS ++G ++++P V IS TGST G L+ + +A ++K Sbjct: 192 LFAEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVTVK 251 Query: 253 KLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRLYVQDGVYDAFAAKLV 312 +++ ELGG +P +V DADL+ A+ +A+ RN GQ+C RL V + Sbjct: 252 RVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVHELAL 311 Query: 313 EAVKKLKVGDGMENGVTQGPLINEQAVKKVEQHIADAVSKGARVLLGGKRHPLGHS---F 369 + ++KVGD + T GPL N +V+Q I + GAR+L GG P G + + Sbjct: 312 ATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDGLAQGYY 371 Query: 370 FEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEAVALANDTEFGLASYFYSRDIGRIW 429 PT+ ++V M +A+EE FGP+ + + T EEA+A+ANDT +GL ++ RD ++ Sbjct: 372 ARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRDKDQVR 431 Query: 430 RVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGRXVGTN 469 VA ++SG V +N + APFGG KQSG GR G Sbjct: 432 AVAARIQSGQVHLNYPAWDPQ-APFGGYKQSGNGREYGVE 470 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,532,934 Number of extensions: 62232 Number of successful extensions: 300 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 255 Number of HSP's successfully gapped: 21 Length of query: 524 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 434 Effective length of database: 1,193,720 Effective search space: 518074480 Effective search space used: 518074480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)