BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0081.p (206 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310406|ref|YP_003774498.1| glutathione S-transferase [Herb... 352 1e-98 gi|300312317|ref|YP_003776409.1| glutathione S-transferase [Herb... 76 2e-15 gi|300311872|ref|YP_003775964.1| glutathione S-transferase famil... 64 8e-12 gi|300313851|ref|YP_003777943.1| glutathione S-transferase [Herb... 58 5e-10 >gi|300310406|ref|YP_003774498.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 206 Score = 352 bits (903), Expect = 1e-98, Method: Compositional matrix adjust. Identities = 179/206 (86%), Positives = 187/206 (90%) Query: 1 MKIHHFSLSGHAHRAVLFASLLGLEAELIHVDLAAGAHKQPAFLTLNPFGQVPVLEDGDI 60 MKIHH LSGHAHRAVLFASLLGL AEL+ VDLAAGAHK+PAFL LNPFGQVPVLEDG++ Sbjct: 1 MKIHHHPLSGHAHRAVLFASLLGLPAELVEVDLAAGAHKKPAFLALNPFGQVPVLEDGEV 60 Query: 61 VIADSNAILVYLAKKAGRTDWLPEDAQGAAAVQRWLSVAAGELAYGPAAARLITVFGARF 120 VIADSNAILVYLAKKAGR+DWLPEDAQGAAAVQRWLSVAAGELAYGPAAARL+TVFGA F Sbjct: 61 VIADSNAILVYLAKKAGRSDWLPEDAQGAAAVQRWLSVAAGELAYGPAAARLVTVFGAAF 120 Query: 121 NPEEVIQRAHLLLGRMEQHLANRAWLAADHPTIADVAVYSYLVAAPEGNVDLSGYPSVLR 180 N EEVIQRAHLLLGR+EQHLA R WLAADHPTIADVAVYSYL APEGNVDL GYP V Sbjct: 121 NAEEVIQRAHLLLGRLEQHLAKRDWLAADHPTIADVAVYSYLARAPEGNVDLRGYPGVQG 180 Query: 181 LLQGIEALPGFVPFPRTAAGLRAAQA 206 LQ IEALPGFVPF +TAAGL AAQA Sbjct: 181 FLQRIEALPGFVPFAQTAAGLMAAQA 206 >gi|300312317|ref|YP_003776409.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 230 Score = 75.9 bits (185), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 55/190 (28%), Positives = 84/190 (44%), Gaps = 15/190 (7%) Query: 11 HAHRAVLFASLLGLEAELIHVDLAAGAHKQPAFLTLNPFGQVPVLEDGD------IVIAD 64 + H+ + GL +D+ AG P FL ++P ++P + D D I + + Sbjct: 11 NGHKVHIMLEECGLPYTAHAIDIGAGDQFTPEFLKISPNNKIPAIVDPDGPDGEPISLFE 70 Query: 65 SNAILVYLAKKAGRTDWLPEDAQGAAAVQRWLSVAAGELAYGPAAA-----RLITVFGAR 119 S AIL+YLA K GR +L E + V +W+ G L GP RL Sbjct: 71 SGAILIYLAGKTGR--FLGETTREKYEVLQWVMFQMGGL--GPMLGQAHHFRLYAPEQIE 126 Query: 120 FNPEEVIQRAHLLLGRMEQHLANRAWLAADHPTIADVAVYSYLVAAPEGNVDLSGYPSVL 179 + A L M++ L A+LA +H TIAD+A + + + +DL +P+V Sbjct: 127 YAVNRYTNEARRLYQVMDKQLGQHAYLAGEHYTIADIACFPWTRSHKNQGIDLDDFPNVK 186 Query: 180 RLLQGIEALP 189 R I A P Sbjct: 187 RWYDAISARP 196 >gi|300311872|ref|YP_003775964.1| glutathione S-transferase family protein [Herbaspirillum seropedicae SmR1] Length = 235 Score = 64.3 bits (155), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 60/191 (31%), Positives = 88/191 (46%), Gaps = 28/191 (14%) Query: 23 GLEAELIHVDLAAGAHKQPAFLTLNPFGQVPVLEDGD------IVIADSNAILVYLAKKA 76 GL E V P FL+LNP ++P + D + + + +S AIL+YLA+K Sbjct: 43 GLPYEAHLVSFETNDQMSPEFLSLNPNNKIPAIIDPNGPGGQPLPLFESGAILLYLAEKT 102 Query: 77 GRTDWLPEDAQGAAAVQRWLSVAAGELAYGPAAARLITVFGARFNPEEV---------IQ 127 G+ +LP DA V +W+ G + GP +L F RF +E + Sbjct: 103 GQ--FLPADAAARYQVIQWVMFQMGGV--GPMFGQL--GFFHRFAGKEYEDKRPRDRYVN 156 Query: 128 RAHLLLGRMEQHLANRAWLAADHPTIADVAVYSY---LVAAPEGNVDLSG---YPSVLRL 181 + LLG M++ LA WL D +IAD+A + + L+ E DL G +P V R Sbjct: 157 ESKRLLGVMDKRLAQSQWLGGDAYSIADIATFPWIRNLIGFYEA-ADLVGFQDFPHVARA 215 Query: 182 LQGIEALPGFV 192 L+ A P V Sbjct: 216 LEAFVARPAVV 226 >gi|300313851|ref|YP_003777943.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 207 Score = 58.2 bits (139), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 51/166 (30%), Positives = 81/166 (48%), Gaps = 10/166 (6%) Query: 1 MKIHHFSLSGHAHRAVLFASLLGLEAELIHVDLAAGAH--KQPAFLTLNPFGQVPVLEDG 58 +KI+ S + + + + LG+ E D AG +PAFL +NP VPV+E+ Sbjct: 2 LKIYGRDSSINVRKVLWACAELGIAFE--REDWGAGFRPTSEPAFLAMNPNAMVPVIEED 59 Query: 59 DIVIADSNAILVYLAKKAGRTDWLPEDAQGAAAVQRWLSVAAGEL--AYGPAAARLITVF 116 V+ +SNAI+ YLA + G P AQ A V +W+ A +L A+ A L+ Sbjct: 60 GFVLWESNAIIRYLANRHGGAALYPLQAQARARVDQWIDWQASDLNRAWSYAYLTLVRKS 119 Query: 117 GARFNPEEV---IQRAHLLLGRMEQHLANR-AWLAADHPTIADVAV 158 A N ++V I + +EQ L + ++A T+AD++V Sbjct: 120 PAHGNAQQVAASIADWTRFMEVLEQQLQHSGGYVAGAEFTLADISV 165 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 685,476 Number of extensions: 28148 Number of successful extensions: 103 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 101 Number of HSP's successfully gapped: 4 Length of query: 206 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 125 Effective length of database: 1,236,335 Effective search space: 154541875 Effective search space used: 154541875 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)