BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0074.p (241 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydroge... 391 e-110 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 123 2e-29 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 120 1e-28 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 112 3e-26 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 105 4e-24 >gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 241 Score = 391 bits (1005), Expect = e-110, Method: Compositional matrix adjust. Identities = 204/241 (84%), Positives = 220/241 (91%) Query: 1 MEFSGTSVIITGAGKGIGRACARLMAQRGAEVIALSRTASDLASLREEIGSRSVQVDLAD 60 M F+G +VIITGAG+GIGRACA L+AQRGA+VIAL+RTASDLA L+EEIG+R + VDL+D Sbjct: 1 MSFAGKTVIITGAGRGIGRACALLLAQRGAQVIALARTASDLARLQEEIGARVIAVDLSD 60 Query: 61 PAAARRAMEQAGTADFLINSAGINVLQSSTEMTDAGYEAVLGVNLRAALITCQAFARARI 120 PAAARRAM +AGTADFLINSAGINVL+S EM+D GYEAVLGVNLRAALITCQAFAR RI Sbjct: 61 PAAARRAMAEAGTADFLINSAGINVLESVLEMSDEGYEAVLGVNLRAALITCQAFARMRI 120 Query: 121 AAGGGGAIVNITSIAGHRGFAEHLCYAASKAGLEGATRVLAKELGAHGIRVNAVAPTITL 180 A GGGGA+VNITSIAGHRGF +HLCYAASKAGLEGATRVLA+ELG HGIRVNAVAPTITL Sbjct: 121 AQGGGGAMVNITSIAGHRGFEQHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTITL 180 Query: 181 TELAEAAWSDPAKSAPMMVRHPLQRFASAEDVARSIAMLLSADAAMLTGAVVPVDGGFLA 240 TELAEAAWSDPAKS PMM RHP RFAS EDVARSIAMLLS DAAML GAVVPVDGGFLA Sbjct: 181 TELAEAAWSDPAKSGPMMARHPSARFASPEDVARSIAMLLSPDAAMLNGAVVPVDGGFLA 240 Query: 241 V 241 V Sbjct: 241 V 241 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 123 bits (308), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 85/247 (34%), Positives = 137/247 (55%), Gaps = 13/247 (5%) Query: 2 EFSGTSVIITGAGKGIGRACARLMAQRGAEVIALSRTASDLASLREEIGS------RSVQ 55 G ++TGA +GIGRA A +A++GA+VI + + S A++ E + + R Sbjct: 5 NLQGQVALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPEAGRGAV 64 Query: 56 VDLADPAAARRAMEQA----GTADFLINSAGINVLQSSTEMTDAGYEAVLGVNLRAALIT 111 +++ D A+ +EQ G+ L+N+AGI Q + M D +++V+ NL A Sbjct: 65 LNVNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRL 124 Query: 112 CQAFARARIAAGGGGAIVNITSIAGHRGFAEHLCYAASKAGLEGATRVLAKELGAHGIRV 171 +A R + A G I+NITS+ G G + YAA+KAG+ G +R LA+E+G+ I V Sbjct: 125 SRAVLRGMMKARTG-RIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNITV 183 Query: 172 NAVAPTITLTELAEAAWSDPAKSAPMMVRHPLQRFASAEDVARSIAMLLSADAAMLTGAV 231 N VAP T++ A + +SA ++ + PL R +AE++A ++A L S AA +TG Sbjct: 184 NCVAPGFIDTDMTRAL--NEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGTT 241 Query: 232 VPVDGGF 238 + V+GG Sbjct: 242 LNVNGGM 248 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 120 bits (301), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 89/241 (36%), Positives = 126/241 (52%), Gaps = 13/241 (5%) Query: 3 FSGTSVIITGAGKGIGRACARLMAQRGAEVIA--LSRTASDLASLREEIGSRS--VQVDL 58 G + ++TG +GIG A AR + GA VIA L A + E GS V++++ Sbjct: 8 LKGKTALVTGGTQGIGAAVARQLVALGARVIAAGLPPFAHE-----EFAGSEVTLVEMNV 62 Query: 59 ADPAAARRAMEQAGTADFLINSAGINVLQSSTEMTDAGYEAVLGVNLRAALITCQAFARA 118 A + R A+ D ++N AGI ++ E+ +E V+ VNL + C A AR Sbjct: 63 ASEESVRAALAGLTQLDIVVNCAGI--IKRGAELETEVFEQVIAVNLTGTMRVCAA-AR- 118 Query: 119 RIAAGGGGAIVNITSIAGHRGFAEHLCYAASKAGLEGATRVLAKELGAHGIRVNAVAPTI 178 + G IVN S+ G Y+ASK G+ T+ LA A GIRVNAVAP Sbjct: 119 ELLRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPGW 178 Query: 179 TLTELAEAAWSDPAKSAPMMVRHPLQRFASAEDVARSIAMLLSADAAMLTGAVVPVDGGF 238 T L +A DP ++ P++ R PL R+ EDV + +A L S A+ +TGA+VPVDGG+ Sbjct: 179 IATPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGAIVPVDGGY 238 Query: 239 L 239 L Sbjct: 239 L 239 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 112 bits (280), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 86/244 (35%), Positives = 133/244 (54%), Gaps = 15/244 (6%) Query: 7 SVIITGAG-KGIGRACARLMAQRGAEVIALSRTASDLASLREEIGSRSVQV--DLADPAA 63 +VI GAG G+G A ARLMA++GA V+ L +D A+ IGS+ + + D+ D A+ Sbjct: 8 AVITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAARIGSQHLGLVADVTDKAS 67 Query: 64 ARRA----MEQAGTADFLINSAGINVLQSSTEMTDAGYEAVLGVNLRAALITCQAFARAR 119 A ++Q G D L+N+AGI + E+T A Y+ +L V+LR L QA A Sbjct: 68 CETAAAAILQQFGRIDILVNNAGITQPVKTLEITGADYDRILDVSLRGTLYMSQAVLPA- 126 Query: 120 IAAGGGGAIVNITSIAGHRG----FAEHLCYAASKAGLEGATRVLAKELGAHGIRVNAVA 175 + A G+I+ I+S++ RG H Y+A+KAG+ G R +A+E G GIRVN++ Sbjct: 127 MRAQQNGSIICISSVSAQRGGGILGGPH--YSAAKAGVLGLARAMAREFGPEGIRVNSIT 184 Query: 176 PTITLTELAEAAWSDPAKSAPMMVRHPLQRFASAEDVARSIAMLLSADAAMLTGAVVPVD 235 P + T++ + ++ K A + PL R A+D+ + L S + TG + V+ Sbjct: 185 PGLIGTDIIKGKLTEE-KKAEIAETIPLARLGRADDIGGACVFLGSDLSQYCTGITLDVN 243 Query: 236 GGFL 239 GG L Sbjct: 244 GGML 247 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 105 bits (261), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 81/245 (33%), Positives = 132/245 (53%), Gaps = 19/245 (7%) Query: 9 IITGAGKGIGRACARLMAQRGAEVI-------ALSRTASDLASLREEIGSRS--VQVDLA 59 ++TG GIG + ++ +A +G V+ A S+TA L + G R+ +Q+DL Sbjct: 12 LVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAA----GHRAAPLQMDLG 67 Query: 60 DP---AAARRAMEQ-AGTADFLINSAGINVLQSSTEMTDAGYEAVLGVNLRAALITCQAF 115 P AAA +E+ G D L+N+AG+ S + + + VN+ L+ Q Sbjct: 68 SPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAGQHA 127 Query: 116 ARARIAAGGGGAIVNITSIAGHRGFAEHLCYAASKAGLEGATRVLAKELGAHGIRVNAVA 175 AR + G IVNI+SI+G R A Y SKA + G TR +A EL +GI VN+VA Sbjct: 128 ARLMVKQQWG-RIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSVA 186 Query: 176 PTITLTELAEAAWSDPAKSAPMMVRHPLQRFASAEDVARSIAMLLSADAAMLTGAVVPVD 235 P T + +A S+ + + + P++R+ + +++A ++ L + ++A +TG V+PVD Sbjct: 187 PGPVDTPMTQAMHSEQTRESYYRLV-PMRRYGTTDEIASAVCYLAAEESAYITGHVIPVD 245 Query: 236 GGFLA 240 GGF+A Sbjct: 246 GGFVA 250 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.130 0.359 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 655,145 Number of extensions: 23224 Number of successful extensions: 325 Number of sequences better than 1.0e-08: 51 Number of HSP's gapped: 236 Number of HSP's successfully gapped: 51 Length of query: 241 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 158 Effective length of database: 1,226,865 Effective search space: 193844670 Effective search space used: 193844670 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)