BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0071.p (243 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310398|ref|YP_003774490.1| ribitol 2-dehydrogenase [Herbas... 485 e-138 gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 95 4e-21 gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogena... 93 2e-20 gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 87 2e-18 gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogena... 86 3e-18 >gi|300310398|ref|YP_003774490.1| ribitol 2-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 243 Score = 485 bits (1249), Expect = e-138, Method: Compositional matrix adjust. Identities = 234/243 (96%), Positives = 238/243 (97%) Query: 1 MPQSLAGKIAVITGAASGIGLATTEKLLANGVTVVMVDWNAKALDELAARLGPQAIPQVT 60 M QSLAGKIAVITGAASGIGLATTEKLLANGVTVVMVDWNAKALDELAARLGPQ IPQVT Sbjct: 1 MTQSLAGKIAVITGAASGIGLATTEKLLANGVTVVMVDWNAKALDELAARLGPQVIPQVT 60 Query: 61 NLLDADSCAAMVPEILAKVDHIDILYCNAGTYIGGELVDTTPEAIDKMLNLNVNAVMKNV 120 NLLDA+SCAAM PEIL KVDHIDILYCNAGTYIGGELVDTTPEAIDKMLNLNVNAVMKNV Sbjct: 61 NLLDAESCAAMAPEILKKVDHIDILYCNAGTYIGGELVDTTPEAIDKMLNLNVNAVMKNV 120 Query: 121 HAVAPHMIARKTGDIIVTCSVAGHFPTYWEPVYAGSKWAITCFVQTMRRQMIPHGVRVGQ 180 HAVAPHM+ RK+GDIIVTCSVAGHFPTYWEPVYAGSKWAITCFVQTMRRQMIPHGVRVGQ Sbjct: 121 HAVAPHMMERKSGDIIVTCSVAGHFPTYWEPVYAGSKWAITCFVQTMRRQMIPHGVRVGQ 180 Query: 181 VSPGPVISALLADWPEENLRKAKESGSLIEPSEVADAIEYMLTRNRNVTIRDMLVLPTNF 240 VSPGPVISALLADWPEENLRKAKESGSLIEPSEVADA+EYMLTRNRNVTIRDMLVLPTNF Sbjct: 181 VSPGPVISALLADWPEENLRKAKESGSLIEPSEVADAVEYMLTRNRNVTIRDMLVLPTNF 240 Query: 241 DRV 243 DRV Sbjct: 241 DRV 243 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 95.1 bits (235), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 69/206 (33%), Positives = 100/206 (48%), Gaps = 16/206 (7%) Query: 5 LAGKIAVITGAASGIGLATTEKLLANGVTVVMVDWNAKALDELAARLGPQAIPQVTNLLD 64 LAGK +IT AA GIG A+TE G V+ D + LDELA G + T+LLD Sbjct: 8 LAGKTVLITAAAQGIGRASTELFAREGARVIATDISKPHLDELAGIAGVE-----THLLD 62 Query: 65 ADSCAAMVPEILAKVDHIDILYCNAGTYIGGELVDTTPEAIDKMLNLNVNAVMKNVHAVA 124 AA + ++AK+ ID+L+ AG G +++ +A D NLN A+ + AV Sbjct: 63 VTDDAA-IKALVAKIGTIDVLFNCAGYVAAGNILECDDKAWDFSFNLNAKAMFHTIRAVL 121 Query: 125 PHMIARKTGDIIVTCSVAGHFPTYWEPV-YAGSKWAITCFVQTMRRQMIPHGVRVGQVSP 183 P M+A+K G I+ S A Y SK A+ +++ + G+R + P Sbjct: 122 PGMLAKKAGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVAQGIRCNAICP 181 Query: 184 GPVISALLADWPEENLR---KAKESG 206 G + S P N R +AKE+G Sbjct: 182 GTIES------PSLNQRISTQAKETG 201 >gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 248 Score = 92.8 bits (229), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 74/244 (30%), Positives = 117/244 (47%), Gaps = 8/244 (3%) Query: 1 MPQSLAGKIAVITGAASGIGLATTEKLLANGVTVVMVDWNAKALDELAARL---GPQAIP 57 M ++ K+ VITGA+SG+G T L + G +V+ + L++L A + G QAI Sbjct: 1 MSNNIKDKVVVITGASSGLGETTARHLASLGAKLVLGARRTERLEKLVADITAAGGQAIA 60 Query: 58 QVTNLLDADSCAAMVPEILAKVDHIDILYCNAGTYIGGELVDTTPEAIDKMLNLNVNAVM 117 T++ D A+V + ID+L NAG + E D+M+++NV V+ Sbjct: 61 VTTDVARRDDVEALVAKGEQHFGRIDVLVNNAGIMPLAPMAKLKVEEWDRMIDVNVKGVL 120 Query: 118 KNVHAVAPHMIARKTGDIIVTCSVAG-HFPTYWEPVYAGSKWAITCFVQTMRRQMIPHGV 176 V A P A+ +G II SVAG VY+ +K+A+ + +R + P GV Sbjct: 121 YGVAAALPRFAAQSSGHIINVSSVAGIKVFAGMGTVYSATKFAVRALTEGLRTEA-PDGV 179 Query: 177 RVGQVSPGPVISALLADWPEENLRKAK---ESGSLIEPSEVADAIEYMLTRNRNVTIRDM 233 R +SPG V S L ++ +A + I VA AI Y + + NV I ++ Sbjct: 180 RTTIISPGAVDSELKTHSSDKETSEALLAWYEANQIPSDSVARAIAYAIEQPANVDISEV 239 Query: 234 LVLP 237 ++ P Sbjct: 240 VLRP 243 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 86.7 bits (213), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 65/237 (27%), Positives = 107/237 (45%), Gaps = 22/237 (9%) Query: 1 MPQSLAGKIAVITGAASGIGLATTEKLLANGVTVVMVDWNAKALDELAARLGPQAIPQVT 60 M + K+ V+TG + GIGLAT + A G V + A L++ +G A+ Sbjct: 3 MAKRFEDKVVVVTGGSEGIGLATAKLFAAEGARVYVSGRRADKLEQAVREIGNGAVGVPG 62 Query: 61 NLLDADSCAAMVPEILAKVDHIDILYCNAGTYIGGELVDTTP------EAIDKMLNLNVN 114 + D + + A +D+L+ NAGT + P E D + NLNV Sbjct: 63 DAAKLDDLDRLYARVQADHGRLDVLFANAGT----ASTEAKPLGMIGEEDFDGLFNLNVR 118 Query: 115 AVMKNVHAVAPHMIARKTGDIIVTCSVAG--HFPTYWEPVYAGSKWAITCFVQTMRRQMI 172 ++ +V P M + G I++ SVAG FP + +Y SK A+ F ++ + Sbjct: 119 GLLFSVQKALPLM--SQGGAIVLNGSVAGVKGFP--GQSLYNASKAAVRSFARSWTADLK 174 Query: 173 PHGVRVGQVSPGPVISALLADWPEEN------LRKAKESGSLIEPSEVADAIEYMLT 223 G+RV V+PG + L+ D+ E L++ G L EP E+ A+ ++ + Sbjct: 175 ERGIRVNVVAPGGTETRLMRDYLEARPGMEDVLKQVVPLGRLGEPDEIGRAVLFLAS 231 >gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 85.5 bits (210), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 69/237 (29%), Positives = 108/237 (45%), Gaps = 26/237 (10%) Query: 1 MPQSLAGKIAVITGAASGIGLATTEKLLANGVTVVMVDWNAKALDELAARL-----GPQA 55 M + L GKIA++TG SGIGLAT ++L A G V + LD A + G +A Sbjct: 1 MNKQLNGKIALVTGGTSGIGLATAQELAAQGAQVFITGRRQAELDAAVASIGAAATGIRA 60 Query: 56 IPQVTNLLDADSCAAMVPEILAKVDHIDILYCNAGTYIGGELV---DTTPEAIDKMLNLN 112 V + LD + +I +DIL+ NAG GG+++ T E D++ N Sbjct: 61 DASVLSELD-----TVYAQIAKSAGRLDILFANAG---GGDMMPLGAITEEHFDRIFGTN 112 Query: 113 VNAVMKNVHAVAPHMIARKTGDIIVTCSVAGHFPTYWEPVYAGSKWAITCFVQTMRRQMI 172 V V+ V P ++ +I+T S T VY+ SK A+ F ++ + Sbjct: 113 VRGVLFTVQKALPLLV--DGASVILTSSTTSVLGTANFSVYSASKAAVRNFARSWALDLK 170 Query: 173 PHGVRVGQVSPGPVISALLADWPEENLRKAKES--------GSLIEPSEVADAIEYM 221 +RV VSPGP+ + L + ++ R+ G L EP E+ A+ ++ Sbjct: 171 DRAIRVNVVSPGPIRTPGLGELVPDDARQGLFDYLASQVPLGRLGEPQEIGKAVAFL 227 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 766,065 Number of extensions: 31723 Number of successful extensions: 228 Number of sequences better than 1.0e-08: 38 Number of HSP's gapped: 192 Number of HSP's successfully gapped: 38 Length of query: 243 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 160 Effective length of database: 1,226,865 Effective search space: 196298400 Effective search space used: 196298400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)