BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0049.p (258 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310380|ref|YP_003774472.1| short-chain alcohol dehydrogena... 494 e-141 gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herb... 99 3e-22 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 98 5e-22 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 96 3e-21 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 93 2e-20 >gi|300310380|ref|YP_003774472.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 494 bits (1272), Expect = e-141, Method: Compositional matrix adjust. Identities = 237/258 (91%), Positives = 249/258 (96%) Query: 1 VDLNLQDKVVIVTGGASGIGGAISLQLAAEGAIPVVFARSEPDPQFWARLTGLQPRAALF 60 +DLNLQDKVVIVTGGASGIG AISLQLAAEGAIPVVFARS P+ QFW RLT +QPRAALF Sbjct: 1 MDLNLQDKVVIVTGGASGIGAAISLQLAAEGAIPVVFARSAPESQFWERLTAVQPRAALF 60 Query: 61 QLELQDEARCGEAVAETVRRFGRLDGLVNNAGVNDSVGLDAGRNEFVASLERNLIHYYVM 120 QLELQD+ARC EAVA T+ RFGRLDGLVNNAGVNDSVGL+AGR+EFVASLERNLIHYYVM Sbjct: 61 QLELQDDARCREAVAGTIERFGRLDGLVNNAGVNDSVGLEAGRDEFVASLERNLIHYYVM 120 Query: 121 AHYCVPHLKATRGAILNVSSKTALTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNA 180 AHYCVPHLKATRGAI+NVSSKTA+TGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNA Sbjct: 121 AHYCVPHLKATRGAIINVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNA 180 Query: 181 LIPAEVMTPLYEKWIATFENPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSGRSSHTT 240 LIPAEVMTPLYEKWIATF++PQEKLDAITSKIPLGKRFTTSEEMADMAVFLLS RSSHTT Sbjct: 181 LIPAEVMTPLYEKWIATFDHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSERSSHTT 240 Query: 241 GQWVFVDGGYTHLDRALT 258 GQW+FVDGGYTHLDRALT Sbjct: 241 GQWIFVDGGYTHLDRALT 258 >gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 369 Score = 99.0 bits (245), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 82/250 (32%), Positives = 123/250 (49%), Gaps = 13/250 (5%) Query: 5 LQDKVVIVTGGASGIGGAISLQLAAEGA-IPVVFARSEPDP-QFWARLTGLQPRAALFQL 62 L KV ++TGG SGIG A++L A EGA + +V+ E D Q + L L Sbjct: 123 LAGKVALITGGDSGIGRAVALAYAREGARLAIVYLDEEEDARQTQKDVQALDAPCLLLPG 182 Query: 63 ELQDEARCGEAVAETVRRFGRLDGLVNNAGVNDSVGL--DAGRNEFVASLERNLIHYYVM 120 ++ C AV + +GRLD LVNNA + + + + N+ + M Sbjct: 183 DVGSPEFCRSAVRAVMDAYGRLDVLVNNAAQQYPAQSIEEISQAQLEHTFRTNIFSMFYM 242 Query: 121 AHYCVPHLKATRGA-ILNVSSKTALTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVN 179 +P L TRGA I+N +S TA G + Y A+KGA ++ TR + L G+ VN Sbjct: 243 VKEAMPFL--TRGARIINTTSVTAYRGSKHLVDYAATKGAIVAFTRSLSQQLASAGIHVN 300 Query: 180 ALIPAEVMTPLYEKWIATFENPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSGRSSHT 239 A+ P + TPL A+F E++ + +PLG R +E+A VFL + +S+ Sbjct: 301 AVAPGPIWTPLIP---ASFS--AEEVASFGKNVPLG-RPGQPDEVAPAYVFLATEGASYM 354 Query: 240 TGQWVFVDGG 249 TGQ + +GG Sbjct: 355 TGQVLHPNGG 364 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 98.2 bits (243), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 74/246 (30%), Positives = 123/246 (50%), Gaps = 9/246 (3%) Query: 8 KVVIVTGGASGIGGAISLQLAAEG-AIPVVFARSEPDPQFWARLTGLQPRAALFQLELQD 66 +V +VTGGA GIG +IS QLAA+G + V + + L RAA Q++L Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAAPLQMDLGS 68 Query: 67 EARCGEAVAETVRRFGRLDGLVNNAGVNDSVG-LDAGRNEFVASLERNLIHYYVMA-HYC 124 A A + +GR D LVNNAGV + LD ++ ++ N+ + H Sbjct: 69 PESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAGQHAA 128 Query: 125 VPHLKATRGAILNVSSKTALTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNALIPA 184 +K G I+N+SS + + + Y SK A + LTR+ A L G+ VN++ P Sbjct: 129 RLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSVAPG 188 Query: 185 EVMTPLYEKWIATFENPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSGRSSHTTGQWV 244 V TP+ + + ++ ++ +P+ +R+ T++E+A +L + S++ TG + Sbjct: 189 PVDTPMTQAM-----HSEQTRESYYRLVPM-RRYGTTDEIASAVCYLAAEESAYITGHVI 242 Query: 245 FVDGGY 250 VDGG+ Sbjct: 243 PVDGGF 248 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 95.9 bits (237), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 82/247 (33%), Positives = 119/247 (48%), Gaps = 9/247 (3%) Query: 5 LQDKVVIVTGGASGIGGAISLQLAAEGAIPVVFARSEPDPQFWARLTGLQP-RAALFQLE 63 + KVVIVTG ASGIG A + + + EGA ++ R L P R A + + Sbjct: 12 FEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAA--ALGKVFDSLPPERTAARETD 69 Query: 64 LQDEARCGEAVAETVRRFGRLDGLVNNAGVNDSVGL-DAGRNEFVASLERNLIHYYVMAH 122 + + + V + RFG+LD LV++AGV + + ++ N+ + A Sbjct: 70 VSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATNVNGVFYGAR 129 Query: 123 YCVPHLKATRGAILNVSSKTALTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNALI 182 +PHL+ TRG I+NV+S + L N S Y ASKGA +LTR A GVR+NA+ Sbjct: 130 EALPHLEKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGVRINAVC 189 Query: 183 PAEVMTPLYEKWIATFENPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSGRSSHTTGQ 242 P+ T + A + LD +I LG R E+A + FL S +S G Sbjct: 190 PSLTHTAM----TADMADDPPLLDKFAERIALG-RGADPLEIAAVITFLASPDASFVNGV 244 Query: 243 WVFVDGG 249 + VDGG Sbjct: 245 NLPVDGG 251 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 93.2 bits (230), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 80/246 (32%), Positives = 116/246 (47%), Gaps = 15/246 (6%) Query: 5 LQDKVVIVTGGASGIGGAISLQLAAEGAIPVVFARSEPDPQFWARLTGLQPRAALFQLEL 64 L+ K +VTGG GIG A++ QL A GA V A P P G + L ++ + Sbjct: 8 LKGKTALVTGGTQGIGAAVARQLVALGA--RVIAAGLP-PFAHEEFAGSE--VTLVEMNV 62 Query: 65 QDEARCGEAVAETVRRFGRLDGLVNNAGVNDSVGLDAGRNEFVASLERNLIHYYVMAHYC 124 E E+V + +LD +VN AG+ G + F + NL + Sbjct: 63 ASE----ESVRAALAGLTQLDIVVNCAGIIKR-GAELETEVFEQVIAVNLTGTMRVCAAA 117 Query: 125 VPHLKATRGAILNVSSKTALTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNALIPA 184 L+ +RG I+N +S + G G GY ASKG LT+ A A DG+RVNA+ P Sbjct: 118 RELLRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPG 177 Query: 185 EVMTPLYEKWIATFENPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSGRSSHTTGQWV 244 + TPL + ++ + I ++ PLG R+ E++ + FL S +S TG V Sbjct: 178 WIATPLTQ----ALQDDPVRAGPILARTPLG-RWGQPEDVGQVVAFLCSPAASFMTGAIV 232 Query: 245 FVDGGY 250 VDGGY Sbjct: 233 PVDGGY 238 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 776,652 Number of extensions: 30535 Number of successful extensions: 219 Number of sequences better than 1.0e-08: 40 Number of HSP's gapped: 160 Number of HSP's successfully gapped: 40 Length of query: 258 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 175 Effective length of database: 1,226,865 Effective search space: 214701375 Effective search space used: 214701375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)