BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0016.p (670 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrog... 1153 0.0 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 283 3e-77 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 261 9e-71 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 257 2e-69 gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrog... 251 9e-68 >gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 793 Score = 1153 bits (2982), Expect = 0.0, Method: Compositional matrix adjust. Identities = 590/658 (89%), Positives = 619/658 (94%), Gaps = 3/658 (0%) Query: 13 YYAGWAQLQESEFPDHVPVGVVGQIVPWNFPLLMLAWKIAPALALGNTVVLKPAEDTSLT 72 +YAGWAQLQESEFPDHVPVGVVGQIVPWNFPLLMLAWKIAPALALGNTVVLKPAE+TSLT Sbjct: 139 HYAGWAQLQESEFPDHVPVGVVGQIVPWNFPLLMLAWKIAPALALGNTVVLKPAENTSLT 198 Query: 73 ALLFAELAQQAGLPAGVLNIVTGDGSTGAAIVAHPGIQKIAFTGSTEVGRLIRAQTAGSG 132 ALLFAELAQQAGLPAGVLNIVTGDG+TGAA+VAHPGIQKIAFTGSTEVGRLIR QTAGSG Sbjct: 199 ALLFAELAQQAGLPAGVLNIVTGDGATGAAVVAHPGIQKIAFTGSTEVGRLIREQTAGSG 258 Query: 133 KSLTLELGGKSPFIVFDDADIDAAIEGVVDAIWFNQGQVCCAGSRLLVQEGIHDLFIARL 192 KSLTLELGGKSPFIVF+DADIDAAIEGVVDAIWFNQGQVCCAGSRLLVQEGIHDLFIARL Sbjct: 259 KSLTLELGGKSPFIVFEDADIDAAIEGVVDAIWFNQGQVCCAGSRLLVQEGIHDLFIARL 318 Query: 193 KKRMEKLKVGAPLDKCSDMGALISPVQLERVRRLVEQGVREGAQCHQVALDVPPGGCFYP 252 K RM+KLKVGAPLDKCSDMGALISPVQLERVR LVEQGVREGAQCHQV LD PPGGCFYP Sbjct: 319 KTRMQKLKVGAPLDKCSDMGALISPVQLERVRSLVEQGVREGAQCHQVVLDTPPGGCFYP 378 Query: 253 PTLLTGVHPAATVATEEIFGPVLVAMSFRTPDEAVQLANNSRYGLAASVWSETIGLALGV 312 PTLLTGVHPAATVA+EEIFGPVLVAMSFRTPDEAVQLANNSRYGLAAS+WSETIGLALGV Sbjct: 379 PTLLTGVHPAATVASEEIFGPVLVAMSFRTPDEAVQLANNSRYGLAASIWSETIGLALGV 438 Query: 313 APQLKAGVVWINSTNQFDAAVGFGGVRESGYGREGGREGCYEYLKPRPRLNRAQRNTASA 372 APQLKAGVVWINSTNQFDAAVGFGGVRESGYGREGGREGCYEYLKPR R SA Sbjct: 439 APQLKAGVVWINSTNQFDAAVGFGGVRESGYGREGGREGCYEYLKPR-RQQAGSLRQPSA 497 Query: 373 TPAVYARATYFPPLQGGLVDRTAKLYIGGKQVRPDGEVSMPCHAASGERLEDVGLGNRKD 432 A+ RA PLQGG +DRTAKLYIGGKQVRPDGEVSM CH+ GERLEDVGLGNRKD Sbjct: 498 RRAI--RADADSPLQGGAIDRTAKLYIGGKQVRPDGEVSMACHSTQGERLEDVGLGNRKD 555 Query: 433 IRNAVAAAVKAGGWSSATAHRRAQGLYYIAENLSARADEFARRIALTTGVSAEAARAEVD 492 IRNAVAAAVKAGGW+SA+ HRRAQGLYYIAENLSAR +EFARRIA TTGV+A+ ARAEVD Sbjct: 556 IRNAVAAAVKAGGWTSASPHRRAQGLYYIAENLSARTEEFARRIASTTGVTADEARAEVD 615 Query: 493 AAIKRLFSYGAWADKYDGAVHQPPLRGVALAMPEAIGVVGVVCPEEQPLLSFISLVAPLI 552 A+IKRLF+YGAWADK++GAVHQPP+RGVALAMPEAIGVVGVVCPEE+PLL+ ISLVAPLI Sbjct: 616 ASIKRLFTYGAWADKFEGAVHQPPMRGVALAMPEAIGVVGVVCPEEKPLLALISLVAPLI 675 Query: 553 AMGNRVVAVPSQQAALVATDLYQVLDTSDLPGGVINIVTGKSAELLTVLAEHDEVDALWV 612 AMGNRVV VPS+QA LVATDLYQVLDTSDLP GVINIVTGKSAELL VLAEHDEV+ALWV Sbjct: 676 AMGNRVVVVPSEQAPLVATDLYQVLDTSDLPAGVINIVTGKSAELLPVLAEHDEVEALWV 735 Query: 613 RGPAEFSATAERLSVGNLKRCFVDHGEVTDWHDAAQAEGSEYLRQATHVKNIWIPYGE 670 RGPAEFSATAERLS GNLKRCFVDHG VTDWHDAAQ EG++YLRQATH+KN+WIPYGE Sbjct: 736 RGPAEFSATAERLSTGNLKRCFVDHGYVTDWHDAAQGEGAQYLRQATHIKNVWIPYGE 793 Score = 67.8 bits (164), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 55/197 (27%), Positives = 83/197 (42%), Gaps = 16/197 (8%) Query: 445 GWSSATAHRRAQGLYYIAENLSARADEFARRIALTTGVSAEAAR-AEVDAAIKRLFSYGA 503 GW + H RA+ LY +A + A A L G +R +V + + Y Sbjct: 83 GWQALGGHGRARHLYALARTVQRHARLLAVLETLDNGKPLRESRDVDVPLVARHFYHYAG 142 Query: 504 WADKYDGAV--HQPPLRGVALAMPEAIGVVGVVCPEEQPLLSFISLVAPLIAMGNRVVAV 561 WA + H P +GVVG + P PLL +AP +A+GN VV Sbjct: 143 WAQLQESEFPDHVP------------VGVVGQIVPWNFPLLMLAWKIAPALALGNTVVLK 190 Query: 562 PSQQAALVATDLYQVLDTSDLPGGVINIVTGKSAELLTVLAEHDEVDALWVRGPAEFSAT 621 P++ +L A ++ + LP GV+NIVTG A V+A H + + G E Sbjct: 191 PAENTSLTALLFAELAQQAGLPAGVLNIVTGDGATGAAVVA-HPGIQKIAFTGSTEVGRL 249 Query: 622 AERLSVGNLKRCFVDHG 638 + G+ K ++ G Sbjct: 250 IREQTAGSGKSLTLELG 266 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 283 bits (725), Expect = 3e-77, Method: Compositional matrix adjust. Identities = 161/367 (43%), Positives = 214/367 (58%), Gaps = 15/367 (4%) Query: 8 ISRARYYAGWAQLQESE--------------FPDHVPVGVVGQIVPWNFPLLMLAWKIAP 53 IS R AGWA E + P+GVVG I+PWN PL++ +WK+A Sbjct: 110 ISHLRAAAGWANQMEGRTIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAA 169 Query: 54 ALALGNTVVLKPAEDTSLTALLFAELAQQAGLPAGVLNIVTGDG-STGAAIVAHPGIQKI 112 LA G TVV+KPAE+T +AL A LAQ+AG P GV+N+VTG G S G A+ HP + KI Sbjct: 170 LLAAGCTVVIKPAEETPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKI 229 Query: 113 AFTGSTEVGRLIRAQTAGSGKSLTLELGGKSPFIVFDDADIDAAIEGVVDAIWFNQGQVC 172 +FTGS E GR I+ K +TLELGGKSP IVFDDA + A+ G ++ NQGQVC Sbjct: 230 SFTGSPEAGRAIQRIAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVC 289 Query: 173 CAGSRLLVQEGIHDLFIARLKKRMEKLKVGAPLDKCSDMGALISPVQLERVRRLVEQGVR 232 AGSR+LVQ + F L + + VG P MG + Q ERV R ++QG+ Sbjct: 290 AAGSRILVQRSLAQRFAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLD 349 Query: 233 EGAQCHQVALDVPPGGCFYPPTLLTGVHPAATVATEEIFGPVLVAMSFRTPDEAVQLANN 292 EGA + P G F PT+ + ++A EEIFGPV +SF T +EA+ LAN+ Sbjct: 350 EGATLLAGGVSRPDKGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALAND 409 Query: 293 SRYGLAASVWSETIGLALGVAPQLKAGVVWINSTNQFDAAVGFGGVRESGYGREGGREGC 352 S+YGLAA+VW+ + A VA +K G V +N + DA + +GG+++SG GREGG G Sbjct: 410 SKYGLAATVWTTNLARAHRVAAGVKVGAVGVNCWSPLDANLPWGGIKDSGMGREGGLAGA 469 Query: 353 YEYLKPR 359 Y + + Sbjct: 470 LAYTEEK 476 Score = 70.5 bits (171), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 59/206 (28%), Positives = 93/206 (45%), Gaps = 11/206 (5%) Query: 441 VKAGGWSSATAHRRAQGLYYIAENLSARADEFARRIALTTGVS-AEAARAEVDAAIKRLF 499 ++ G WS + +R Q L+ +A + D A A G S E +V AI L Sbjct: 55 LEGGAWSKLSGLQRGQLLHKLAALVERDTDLLADMDARAIGRSPMEPRMMDVPNAISHLR 114 Query: 500 SYGAWADKYDGA-------VHQPPLRGVALAMPEAIGVVGVVCPEEQPLLSFISLVAPLI 552 + WA++ +G + +P L + + E IGVVG + P PL+ VA L+ Sbjct: 115 AAAGWANQMEGRTIPTAGYMGKPTL---SYTVREPIGVVGAILPWNAPLMITSWKVAALL 171 Query: 553 AMGNRVVAVPSQQAALVATDLYQVLDTSDLPGGVINIVTGKSAELLTVLAEHDEVDALWV 612 A G VV P+++ A L ++ + P GVIN+VTG + L EH +V + Sbjct: 172 AAGCTVVIKPAEETPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISF 231 Query: 613 RGPAEFSATAERLSVGNLKRCFVDHG 638 G E +R++ KR ++ G Sbjct: 232 TGSPEAGRAIQRIAGEQFKRVTLELG 257 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 261 bits (668), Expect = 9e-71, Method: Compositional matrix adjust. Identities = 143/327 (43%), Positives = 205/327 (62%), Gaps = 1/327 (0%) Query: 31 VGVVGQIVPWNFPLLMLAWKIAPALALGNTVVLKPAEDTSLTALLFAELAQQAGLPAGVL 90 +GV I PWNFP M+ K PALA G +VLKPAE T +AL A LA++AG+PAGV Sbjct: 148 IGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALALAVLAERAGIPAGVF 207 Query: 91 NIVTGD-GSTGAAIVAHPGIQKIAFTGSTEVGRLIRAQTAGSGKSLTLELGGKSPFIVFD 149 ++VTG G + ++P ++KI+FTGST VG+L+ Q+A S K L+LELGG +PFIVFD Sbjct: 208 SVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKKLSLELGGNAPFIVFD 267 Query: 150 DADIDAAIEGVVDAIWFNQGQVCCAGSRLLVQEGIHDLFIARLKKRMEKLKVGAPLDKCS 209 DAD+DAA+EG + + + N GQ C +R+ VQ+G++D F A+L + ++K KVG ++ Sbjct: 268 DADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVEAVKKFKVGQGTEEGV 327 Query: 210 DMGALISPVQLERVRRLVEQGVREGAQCHQVALDVPPGGCFYPPTLLTGVHPAATVATEE 269 G LI+ +++V + V V +GA+ G F+ PT+L V PA VA EE Sbjct: 328 TQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALGHSFFEPTVLADVTPAMQVAREE 387 Query: 270 IFGPVLVAMSFRTPDEAVQLANNSRYGLAASVWSETIGLALGVAPQLKAGVVWINSTNQF 329 FGP+ F+T +E + LAN++ +GLA+ +S IG VA L++G+V IN+ Sbjct: 388 TFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRVAEGLESGMVGINTGLIS 447 Query: 330 DAAVGFGGVRESGYGREGGREGCYEYL 356 + FGGV++SG GREG G +YL Sbjct: 448 NEVAPFGGVKQSGLGREGSHYGIDDYL 474 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 257 bits (656), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 157/351 (44%), Positives = 203/351 (57%), Gaps = 17/351 (4%) Query: 13 YYAGWAQLQESEFPDHVPVGVVGQIVPWNFPLLMLAWKIAPALALGNTVVLKPAEDTSLT 72 Y AG+ L E P GV G IVPWN+P+ + + ALA GNT V+KPAED L+ Sbjct: 125 YQAGYTVLTLRE-----PHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLS 179 Query: 73 ALLFAELAQQAGLPAGVLNIVTGDG-STGAAIVAHPGIQKIAFTGSTEVGRLIRAQTAGS 131 L AELA + G P G LNIVTG G G +V HP + I+FTGS VG+L+ A Sbjct: 180 ILRVAELAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVNHISFTGSPNVGKLVVRAAAEH 239 Query: 132 GKSLTLELGGKSPFIVFDDADIDAAIEGVVDAIWFNQGQVCCAGSRLLVQEGIHDLFIAR 191 +TLELGGKSP IVF+DAD++A + VV+AI N GQ C AGSRLLVQ G ++ + R Sbjct: 240 HTPVTLELGGKSPQIVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQVLER 299 Query: 192 LKKRMEKLKVGAPLDKCSDMGALISPVQLERVRRLVEQGVREG---AQCHQVALDVPPGG 248 L +L+VG+ D G LI Q ERV + R+G A ++A VP GG Sbjct: 300 LSGLFSQLQVGSSQMDL-DCGPLIRKTQQERVAGFLSDAQRDGIALAAQGKIAAGVPTGG 358 Query: 249 CFYPPTLLTGVHPAATVATEEIFGPVLVAMSFRTPDEAVQLANNSRYGLAASVWSETIGL 308 + P LL V P +A EE+FGPVL AM F A+ LAN + YGL ASVW+ G Sbjct: 359 FYQAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVASVWTRDGGR 418 Query: 309 ALGVAPQLKAGVVWINSTNQFDAAVG----FGGVRESGYGREGGREGCYEY 355 + +A +++G V+IN+ + A G FGGV+ SG+GRE G E Y + Sbjct: 419 QMRLARAIRSGQVFINN---YGAGGGVELPFGGVKASGHGREKGFEALYGF 466 Score = 74.3 bits (181), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 62/220 (28%), Positives = 94/220 (42%), Gaps = 3/220 (1%) Query: 398 YIGGKQVRPDGEVSMPC-HAASGERLEDVGLGNRKDIRNAVAAAVKA--GGWSSATAHRR 454 +I + V P S+P + G+ + + GN DI AV AA A G W +A R Sbjct: 5 FIANRWVAPTSGESIPVLDPSDGQPFDAIARGNATDIDQAVRAARLAYEGAWGRLSAAER 64 Query: 455 AQGLYYIAENLSARADEFARRIALTTGVSAEAARAEVDAAIKRLFSYGAWADKYDGAVHQ 514 ++ L +A L +E A+ G + ARA+ A ++ Y ADK G Sbjct: 65 SRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARADAAAIVRYFEFYAGAADKLHGDTIP 124 Query: 515 PPLRGVALAMPEAIGVVGVVCPEEQPLLSFISLVAPLIAMGNRVVAVPSQQAALVATDLY 574 L + E GV G + P P+ F V +A GN V P++ A L + Sbjct: 125 YQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSILRVA 184 Query: 575 QVLDTSDLPGGVINIVTGKSAELLTVLAEHDEVDALWVRG 614 ++ P G +NIVTG E +L +H +V+ + G Sbjct: 185 ELAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVNHISFTG 224 >gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 504 Score = 251 bits (642), Expect = 9e-68, Method: Compositional matrix adjust. Identities = 149/377 (39%), Positives = 205/377 (54%), Gaps = 13/377 (3%) Query: 13 YYAGWAQLQESE----------FPDHVPVGVVGQIVPWNFPLLMLAWKIAPALALGNTVV 62 YYAGWA E + P G V Q+ PWN P W++ PALA GN V+ Sbjct: 129 YYAGWADKFYGEVIPVPSSHLNYTRREPYGTVLQMTPWNAPAFTCGWQLGPALATGNAVL 188 Query: 63 LKPAEDTSLTALLFAELAQQAGLPAGVLNIVTGDGSTGA-AIVAHPGIQKIAFTGSTEVG 121 LKP+E T ++L A L +QAGLPAG++N++ G G T +A ++K+ F GS G Sbjct: 189 LKPSELTPFSSLAIARLGEQAGLPAGLVNVLAGLGQTMVPQAMATWTVKKVIFVGSPATG 248 Query: 122 RLIRAQTAGSGKSLTLELGGKSPFIVFDDADIDAAIEGVVDAIWFNQGQVCCAGSRLLVQ 181 LI A LELGGKS I+F+DAD+ A G AI+ GQ C AGSRLLVQ Sbjct: 249 ALIAKAAAARVMPCVLELGGKSANIIFEDADLRLAAFGAQAAIFSGAGQSCVAGSRLLVQ 308 Query: 182 EGIHDLFIARLKKRMEKLKVGAPLDKCSDMGALISPVQLERVRRLVEQGVREGAQCHQVA 241 ++D F+ + EK+++GAPLD +++G + + Q E ++R+V +G+ GA Sbjct: 309 RKVYDRFVETVAAGAEKIRLGAPLDDSTEVGPINNRKQYEHIQRMVARGLEAGATLAAGH 368 Query: 242 LDVPPGGCFYPPTLLTGVHPAATVATEEIFGPVLVAMSFRTPDEAVQLANNSRYGLAASV 301 G F PTLL A VA EIFGPV VA+ F +EA+ +AN+S +GLA +V Sbjct: 369 TRYGEEGYFVRPTLLAHASNAMEVARSEIFGPVAVAIPFEDEEEAIAIANDSEFGLAGAV 428 Query: 302 WSETIGLALGVAPQLKAGVVWINSTNQFDAAVGFGGVRESGYGREGGREGCYEYLKPRPR 361 W+ + A VA + AG W+NS + A FGG SGYGR G E Y+Y + + Sbjct: 429 WTRDVARAHRVAASVNAGTFWVNSYKTINVASPFGGFNRSGYGRSSGMEALYDYTQTKSV 488 Query: 362 LNRAQRNTASATPAVYA 378 + + + ATP YA Sbjct: 489 W--VETSASPATPFGYA 503 Score = 59.7 bits (143), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 38/147 (25%), Positives = 67/147 (45%) Query: 446 WSSATAHRRAQGLYYIAENLSARADEFARRIALTTGVSAEAARAEVDAAIKRLFSYGAWA 505 W + + R + LY + + A A+ AR A+++G RAE+ + Y WA Sbjct: 75 WWALSHAARGRALYAVGAVIRAEAEPLARLEAISSGKPIRDCRAEMQKVAEMFEYYAGWA 134 Query: 506 DKYDGAVHQPPLRGVALAMPEAIGVVGVVCPEEQPLLSFISLVAPLIAMGNRVVAVPSQQ 565 DK+ G V P + E G V + P P + + P +A GN V+ PS+ Sbjct: 135 DKFYGEVIPVPSSHLNYTRREPYGTVLQMTPWNAPAFTCGWQLGPALATGNAVLLKPSEL 194 Query: 566 AALVATDLYQVLDTSDLPGGVINIVTG 592 + + ++ + + LP G++N++ G Sbjct: 195 TPFSSLAIARLGEQAGLPAGLVNVLAG 221 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,152,810 Number of extensions: 93705 Number of successful extensions: 412 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 361 Number of HSP's successfully gapped: 34 Length of query: 670 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 578 Effective length of database: 1,184,250 Effective search space: 684496500 Effective search space used: 684496500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)