BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0014.p (320 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310353|ref|YP_003774445.1| ribokinase [Herbaspirillum sero... 565 e-162 gi|300311612|ref|YP_003775704.1| sugar kinase [Herbaspirillum se... 150 9e-38 gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family ... 86 3e-18 gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase [Herbasp... 79 6e-16 >gi|300310353|ref|YP_003774445.1| ribokinase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 565 bits (1457), Expect = e-162, Method: Compositional matrix adjust. Identities = 295/319 (92%), Positives = 303/319 (94%) Query: 1 MTSLSHKTGVAVLGIYVADLAFRAPGMPGLGQTIAGTGFAMGPGGKGSNQAVAAARVGAP 60 MTS HK GVAVLGIYVADLAFRAP MPGLGQTIAGTGFAMGPGGKGSNQAVAAARVGAP Sbjct: 1 MTSFPHKQGVAVLGIYVADLAFRAPNMPGLGQTIAGTGFAMGPGGKGSNQAVAAARVGAP 60 Query: 61 VRFISAIGKDSFGDFALSLWRQETIAPHVRVIEDAPTGAAFIYVNDASGDNAIIVVPGAA 120 VRFISAIGKD+FGDFALSLWR+ETIAPHVRVI+ APTGAAFIYVNDASGDNAIIVVPGAA Sbjct: 61 VRFISAIGKDAFGDFALSLWRRETIAPHVRVIDGAPTGAAFIYVNDASGDNAIIVVPGAA 120 Query: 121 SQLSTQDVEREHQAIEAASVFVTQLEQPPEAALTGLQIARAAGTTTVFNPAPALDFPPQI 180 SQLS QDVERE QAIEAA VFVTQLEQPPEAAL GLQIARAAGTTTVFNPAPAL FP Q+ Sbjct: 121 SQLSEQDVERERQAIEAAKVFVTQLEQPPEAALAGLQIARAAGTTTVFNPAPALAFPAQV 180 Query: 181 YGLCDFITPNEHEAALLTGIEIHTVDDARRAADVLLERGVGCALITLGAQGALLHSRSQS 240 YGLCDFITPNEHEAALLTGIEIH +DDARRAADVLLERGVGCALITLGAQG+LLHSRSQS Sbjct: 181 YGLCDFITPNEHEAALLTGIEIHHIDDARRAADVLLERGVGCALITLGAQGSLLHSRSQS 240 Query: 241 LHLPALCAGTVVETAGAGDAFNGAFAAGLAEGMSAEQAARLATAVAAISVTRPGTAPSMP 300 LHLPALCAGTVVETAGAGDAFNGAFAAGLAEGMSAE+AARLATAVAAISVTRPGTAPSMP Sbjct: 241 LHLPALCAGTVVETAGAGDAFNGAFAAGLAEGMSAEEAARLATAVAAISVTRPGTAPSMP 300 Query: 301 SRQEALALLQANAGALAQA 319 SRQEALALL +A LAQA Sbjct: 301 SRQEALALLDTHASVLAQA 319 >gi|300311612|ref|YP_003775704.1| sugar kinase [Herbaspirillum seropedicae SmR1] Length = 299 Score = 150 bits (380), Expect = 9e-38, Method: Compositional matrix adjust. Identities = 113/306 (36%), Positives = 158/306 (51%), Gaps = 18/306 (5%) Query: 10 VAVLGIYVADLAFRAPGMPGLGQTIAGTGFAMGPGGKGSNQAVAAARVGAP---VRFISA 66 + ++G DL R P MP G+T+AG F PGGKG+NQAVA AR+ AP V ++ Sbjct: 2 IVIIGSVNMDLVLRVPRMPLPGETLAGDRFMTIPGGKGANQAVACARLAAPGTRVAMVAC 61 Query: 67 IGKDSFGDFALSLWRQETIAPHV--RVIEDAP---TGAAFIYVNDASGDNAIIVVPGAAS 121 +G D+FG RQ A + R I++ TG A I V DA+ N+I++ GA Sbjct: 62 VGDDAFG----GQMRQSITACGIDDRYIDEVAGEATGIASIMV-DANAQNSIVIAAGANG 116 Query: 122 QLSTQDVEREHQAIEAASVFVTQLEQPPEAALTGLQIARAAGTTTVFNPAPALDFPPQIY 181 +L + +ER IE AS+ + QLE P + +++A A G T V NPAPA P + Sbjct: 117 RLDVERIERARALIEQASIVLLQLEVPMATVIHSIELAHALGKTVVLNPAPAQALPRALL 176 Query: 182 GLCDFITPNEHEAALLTGIEIHTVDDARRAADVLLERGVGCALITLGAQGAL-LHSRSQS 240 D++ NE EAA+L +D A L + G ++TLG +G + Q Sbjct: 177 QKIDYLILNEIEAAMLAE---EQSEDIPMLARKLHDLGARNVVVTLGEKGVYGSFADGQQ 233 Query: 241 LHLPALCAGTVVETAGAGDAFNGAFAAGLAEGMSAEQAARLATAVAAISVTRPGTAPSMP 300 HLPA V+T AGD F G F +A+G +A A A AA+SVTR G S+P Sbjct: 234 RHLPARKV-QAVDTTAAGDTFIGGFIGAIAQGRDQFEAIAYAQAAAALSVTRVGAQTSIP 292 Query: 301 SRQEAL 306 +R E + Sbjct: 293 TRDEVV 298 >gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 86.3 bits (212), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 97/292 (33%), Positives = 136/292 (46%), Gaps = 24/292 (8%) Query: 39 FAMGPGGKGSNQAVAAARVGAPVRFISAIGKDSFGDFALSLWRQETIAPHVRVIEDAPTG 98 F G GG SN A+AAAR GA ++SA+G D FG L+LW++E + VR + AP G Sbjct: 25 FLQGYGGDTSNFAIAAARQGAASAYLSAVGDDHFGQDLLALWQREEV--DVRHVAIAPGG 82 Query: 99 AAFIY--VNDASGDNAIIVVPG-AASQLSTQDVERE----HQAIEAASVFVTQLEQPPEA 151 + IY +DA+G + G AAS + + E A+ + + + E EA Sbjct: 83 STGIYFVTHDAAGHHFHYRRAGSAASAYQAKALPLEAIAAAGALHLSGISLAISESACEA 142 Query: 152 ALTGLQIARAAGTTTVFNPAPALDFPP---------QIYGLCDFITPNEHEAALLTGIEI 202 L + ARA+G TT + L P + + LCD P+ + +LLTG+E Sbjct: 143 GLAAMAHARASGVTTSLDTNLRLRLWPLERARARMEEAFRLCDICLPSWEDVSLLTGLE- 201 Query: 203 HTVDDARRAADVLLERGVGCALITLGAQGALLHSRSQSLHLPALCAGTVVETAGAGDAFN 262 D D LL GV LG +G + + Q + A ++ GAGD F Sbjct: 202 ----DRDAIVDRLLSYGVKLVAFKLGKEGCYVATSDQR-RMVAPYPVDSLDATGAGDCFG 256 Query: 263 GAFAAGLAEGMSAEQAARLATAVAAISVTRPGTAPSMPSRQEALALLQANAG 314 GAF A L G A AAR A AA+S T G +P R + A+L +G Sbjct: 257 GAFMAELMAGRDAFAAARYANVAAALSTTGYGAVEPIPRRAQVEAVLAKTSG 308 >gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase [Herbaspirillum seropedicae SmR1] Length = 313 Score = 78.6 bits (192), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 83/279 (29%), Positives = 116/279 (41%), Gaps = 26/279 (9%) Query: 45 GKGSNQAVAAARVGAPVRFISAIGKDSFGDFALSLWRQETIAPHVRVIEDAPTGAAFIYV 104 G +N A+ AR+G V + S +G D+FG F QE + RVI D A Sbjct: 37 GAETNVAIGLARLGLKVGWASRVGNDAFGRFIRQRVAQEGVEVS-RVITDMEFRTAIQLK 95 Query: 105 NDASG--DNAIIVVP--GAASQLSTQDVEREH----QAIEAASVFVTQLEQPPEAALTGL 156 A G D AI AAS LS D + + + + A + A + Sbjct: 96 AKAVGGADPAIEYYRKGSAASHLSVDDFDAGYFGAARHLHATGIAPALSATTMAFAHQAM 155 Query: 157 QIARAAGTTTVFNP--APALDFPPQ---------IYGLCDFITPNEHEAALLTGIEIHTV 205 R G T F+P P L +P Q + D++ P E +LTG H Sbjct: 156 DFMRGQGKTISFDPNLRPML-WPSQEVMAQQLNALAFKADWVLPGLSEGKILTG---H-- 209 Query: 206 DDARRAADVLLERGVGCALITLGAQGALLHSRSQSLHLPALCAGTVVETAGAGDAFNGAF 265 DDAR A LERGV +I LGA+GA + + + VV+T GAGD F Sbjct: 210 DDAREIAGFYLERGVKLVVIKLGAEGAYWRNGQGEGRVAGVPVKEVVDTVGAGDGFAVGV 269 Query: 266 AAGLAEGMSAEQAARLATAVAAISVTRPGTAPSMPSRQE 304 +G+ EG+ QA + A ++ G +P+R E Sbjct: 270 ISGMLEGLPVPQAVMRGNRIGAFAIQVVGDMEGLPTRAE 308 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.131 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 904,403 Number of extensions: 35728 Number of successful extensions: 192 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 186 Number of HSP's successfully gapped: 5 Length of query: 320 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 235 Effective length of database: 1,217,395 Effective search space: 286087825 Effective search space used: 286087825 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)